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Citations for R&D Systems :
3001 - 3050 of 3214 citations for 8 1 Piperazinyl imidazo 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 0.1% NaN3 in D-PBS) containing optimal concentration determined by antibody titration of the Alexa Fluor® 647-conjugated IGDCC3 extracellular antibody (1:20; R&D Systems; Cat. No. FAB8559R). After a 30 min incubation in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
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bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Genetics 2024Quote: The concentration of IGF-1 in the liver was quantified using the Mouse/Rat IGF-I/IGF1 Quantikine ELISA Kit (MG100, R&D Systems, Inc., Minneapolis, MN, USA). Liver protein lysates were diluted at a ratio of 1:6 in calibrator diluent and the assay was performed following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... Organoid basal growth media was supplemented with epidermal growth factor (EGF) (100 ng/mL, 10 ng/mL, 1 ng/mL R&D Systems Cat#236-EG-01M) or Epiregulin (EREG ...
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bioRxiv - Neuroscience 2024Quote: Immunohistochemical staining for ADIPOR1 and MFRP was conducted on tissues that were fixed in formalin and embedded in paraffin. The antibodies were rabbit anti-Adiponectin Receptor 1 (IBL, Catalog No. 18993, Japan) and goat anti-mouse MFRP (R&D Systems, Catalog No. BAF3445, Minnesota) respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary incubation was 1 hour at RT in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, HAF007 and HAF008, respectively). The suspension for blocking and antibody incubations was 1x Tris buffered saline (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Recombinantly expressed TIM-4 in HEK293 cells (Q96H15) and podocalyxin-like protein 1 recombinantly expressed in NS0 cells (AAB61574.1) were purchased from R&D Systems (9407-TM, 1658-PD-050). Recombinantly expressed LAMP-4 in HEK293 cells (P34810 ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution). After incubation with primary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... lymph node tissue sections derived from mice bearing pancreatic tumors were incubated overnight at RT with the following primary antibodies: NKp46 (R&D systems AF2225, used at 1:25), Ki67 (Abcam ab15580 ...
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bioRxiv - Molecular Biology 2020Quote: ... SH-SY5Y and SK-N-AS cells were pre-treated for 16 h with 1000 Units/ml Interferon Type 1 (Interferon α/β, R&D Systems (Minneapolis, MN, USA)) followed by 2,5 μM BV6 (Selleckchem ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution), followed by incubation with primary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were embedded in Cultrex® RGF BME Type 2 (cat no. 3533-005-02, R&D systems) and placed in a 37°C incubator for 20 min ...
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bioRxiv - Genomics 2019Quote: ... the medium was changed to STEMdif APEL 2 with 200 ng/mL FGF9 (R&D Systems, Minneapolis, MN) and 1 mg/mL heparin (StemCell Technologies ...
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bioRxiv - Immunology 2019Quote: Myeloperoxidase (MPO) and Lipocalin-2 were measured using ELISAs and following the manufacturers’ recommendations (from R&D Systems).
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bioRxiv - Microbiology 2021Quote: ... and FABP-2 were measured from plasma using standard ELISA kits from R&D Systems (DC140, DC1630 &DFBP20). Positive testing controls for each ELISA kit were also included (R&D Systems QC20 ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... or recombinant human cathepsin L (CatL, 2 ng/ul, pH 5.5, 37°C for 1h; R&D Systems) as described previously (5) ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse tumors were homogenized with a tissue homogenizer in 700μL of Cell Lysis Buffer 2 (R&D Systems) containing 1x Halt Protease Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: Mature adipocytes were cultured in complete medium supplemented with AZA (R&D Systems; 5, 10, 20µM; 2 days) and rhPDGF-AB (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2022Quote: ... PBS was removed and 50 μl of cell lysis buffer (Cell Lysis Buffer 2, R&D Systems, # 895347) were added ...
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bioRxiv - Cancer Biology 2022Quote: For recombinant E6 proteins and sdAbs: 2 µg of E6 (R&D Systems Inc., Cat. # AP-120-025), MBP (Abnova ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were maintained in proliferating state using 10 ng/ml fibroblast growth factor 2 (FGF2; R&D Systems) and passaged twice before usage in the experiments ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were grown in DMEM/F12 (HyClone, SH30023.02) supplemented with 2 %v/v N21 (R&D system, AR008), 20 ng/mL EGF (R&D system ...
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bioRxiv - Immunology 2022Quote: ... all necessary diluents and buffers were from the DuoSet® Ancillary Reagent Kit 2 (R&D Systems, #DY008). The assays were performed at RT unless otherwise noted ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA), and in the presence or the absence of CH-223191 or FICZ ...
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bioRxiv - Neuroscience 2022Quote: ... Human TDP-43 DuoSET ELISA kit DuoSet Ancillary Reagent Kit 2 (#DY008, R&D Systems, Minneapolis, MN, USA), and tetraspanin exosome marker CD81 (CSB-EL004960HU ...
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bioRxiv - Pathology 2024Quote: ... Cell surface protein expression was performed by labeling 2×105 cells for KDR-PE (R&D Systems, FAB357P) + PDGFRα-APC (Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... we detected C5a with 2 μg/ml anti-C5a detection antibody (C5a DuoSet ELISA kit, R&D systems), followed by an incubation of 1:5000 streptavidin-HRP (Southern Biotech) ...
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bioRxiv - Immunology 2023Quote: About 200 colonic crypts were seeded in 25 μL Cultrex BME Type 2 (R&D systems, Minneapolis, MN) domes and cultured in Advanced DMEM/ F-12 supplemented with N2 ...
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bioRxiv - Immunology 2023Quote: ... Using a SARS-CoV-2 spike RBD mAb of known concentration (MAB10580; R&D Systems, Minneapolis, Minnesota, USA) as a molecular standard ...
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bioRxiv - Immunology 2024Quote: ... NK cells were cultured in RP10 supplemented with 300 IU/ml recombinant human IL-2 (R&D Systems) for 72 h unless otherwise noted ...
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bioRxiv - Developmental Biology 2024Quote: ... were washed in 1X PBS then incubated in goat anti-GAS1 (2 mg, R&D Systems; Cat#AF2644) antibody diluted in 1X PBS for 2 h at room temperature on a rotating platform ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were stimulated with 2 ng/mL recombinant human TGF-β1 (R&D Systems, cat. # 240-B) diluted in starvation medium for varying durations of 30 ...
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bioRxiv - Physiology 2024Quote: ... Protein expression was visualised using immunofluorescence staining of mesenteric artery rings and the following antibodies: anti-CD31 (PECAM, # AF3628, R&D Systems, 1:1000 dilution, raised in goat); anti-TRPV4R (# ACC-034 ...
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bioRxiv - Cancer Biology 2023Quote: To measure IFNγ secretion by the T cells co-cultured with the tumor-derived macrophages in the media of the T cell suppression assay we used Quantikine™ ELISA Mouse IFN-γ Immunoassay (R&D Systems, catalog number #MIF00-1) according to manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... Dimeric IgA in BAL and nasal swabs was measured using DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) as described before20 ...
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bioRxiv - Molecular Biology 2020Quote: ... FGF-2 in the CM was assayed using a human FGF basic DuoSet ELISA Development Kit (R&D Systems) according to the manufacturer’s procedure.
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bioRxiv - Immunology 2021Quote: ... half-medium exchange was performed and 10 IU/mL IL-2 (R&D Systems; Cat no 202-IL-500) added ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 5000 RU of Recombinant Human Desmoglein-2 Fc Chimera Protein (R&D Systems, Catalogue number 947-DM-100) was amine coupled to a CM5 sensor chip at a slow flow-rate of 10 μl/min to ensure uniform distribution on the chip surface.
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bioRxiv - Immunology 2022Quote: ... T cells then were washed and further incubated with recombinant human IL-2 (10 ng/ml; R&D systems) for up to 2 weeks at 37°C incubator with medium change every 3 days ...
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bioRxiv - Immunology 2021Quote: ... mice were injected subcutaneously with IL-4 complex (IL-4c) comprised of rmIL-4 (2 μg, R&D systems) combined with anti-IL-4 antibody 10 μg ...
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bioRxiv - Immunology 2020Quote: ... 2-incubation with 0.5 μg/mL primary anti mouse-TLR4 (recombinant monoclonal rabbit IgG, clone #1203B, R&D Systems) in microscopy buffer for 1 h at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... An ELISA for mouse lipocalin-2 was performed on feces (protocol according to the manufacturer; R&D Systems kit) to determine the inflammatory state of the gut.
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bioRxiv - Immunology 2022Quote: ... S proteins used in the study were SARS-CoV-2 S GCN4-IZ-2P (10561-CV, R&D Systems), SARS-CoV-2 B.1.1.529 S GCN4-IZ-2P (11061-CV ...
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bioRxiv - Molecular Biology 2022Quote: ... CH12F3-2 cells were incubated in culture medium supplemented with10ng/mL IL-4 (R&D Systems #404-ML-050), 1ng/mL TGFβ (R&D Systems #7666-MB-005 ...
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bioRxiv - Microbiology 2024Quote: ... was measured from the resulting supernatant using the Duoset murine Lcn-2 ELISA kit (R&D Systems, Minneapolis, MN). Measuring lipocalin-2 (Lcn-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Both PDO lines were maintained in growth factor reduced type 2 Cultrex (Biotechne/R&D Systems, 3533-010-02) with phenol red-free DMEM/F-12 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated with compounds for 2 hours prior to TGF-β1 stimulation (1ng/ml, R&D systems. After 48 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and the medium was supplemented with 10 ng/ml rhFGF-2 and 20 ng/ml VEGF (R&D Systems) for EC expansion ...