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Citations for R&D Systems :
3051 - 3100 of 3214 citations for 8 1 Piperazinyl imidazo 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and the medium was supplemented with 10 ng/ml rhFGF-2 and 20 ng/ml VEGF (R&D Systems) for EC expansion ...
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... 10 ng/mL of IL-2 and 10 ng/mL of IL-12 (R&D systems, 419-ML-010)) ...
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bioRxiv - Immunology 2023Quote: ... MRT organoids were cultured in droplets of growth factor-reduced basement membrane extract (BME) Type 2 (R&D Systems) in kidney organoid medium (KOM ...
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bioRxiv - Cell Biology 2023Quote: ... starved for 2 hours and treated with 10 ng/ml rat recombinant EGF (R&D Systems, Minneapolis, MN, USA) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... to small clumps and plated on a layer of BME type 2 (R&D Systems, cat. no. 3533-001), incubated overnight in culture medium supplemented with 10 μg/mL polybrene (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2023Quote: ... mouse IFNβ (catalog # 42400-2) cytokine levels were measured using ELISA kits from R&D Systems (Minneapolis, MN, USA). Human IFNβ levels were measured using an ELISA kit (PBL Assay Science ...
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bioRxiv - Neuroscience 2023Quote: ... and the growth factors FGF-2 (20 ng/ml, Alomone Labs) and EGF (20 ng/mL, R&D systems). NSC differentiation was induced withdrawal of these growth factors.
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bioRxiv - Cancer Biology 2024Quote: ... Each microwell was then overlaid with 2 μL of organoid digestion mix including Organoid Harvesting Solution (R&D Systems) with 10 mg/mL B ...
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bioRxiv - Immunology 2024Quote: ... The binding kinetics for the interaction between hmAbs and SARS-CoV-2 Spike protein (R&D Systems, 10549-cv) was determined by injecting two to four concentrations of SARS-CoV-2 RBD (50 nM highest concentration) ...
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bioRxiv - Neuroscience 2023Quote: ... A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat # 280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were kept in 1.5ml Eppendorf tube and stimulated by adding 50nM CCL19 (R&D System 440-M3) and 50nM CCL21 (R&D System 457-6C ...
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bioRxiv - Neuroscience 2020Quote: ... The growth medium was replaced with DMEM supplemented with 2 ng/mL murine M-CSF (R&D Systems; Minneapolis, MN) and cultured in Nx for 3 or 6 days prior to LPS treatment.
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bioRxiv - Immunology 2022Quote: ... cells were washed at day 7 and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 250 U/ml IFN-α (R&D Systems) ...
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bioRxiv - Developmental Biology 2022Quote: ... For the 2-day protocol in which cells were switched from Wnt3a to Activin A (R&D Systems, Minneapolis, MN), the samples were washed with PBS +/+ before adding fresh medium ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... organoids were grown embedded in a mix of 90% ice-cold RGF BME Type 2 PathClear (Cultrex, R&D Systems) and 10% Dulbecco’s modified Eagle’s Medium/Ham’s F12 (Gibco) ...
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bioRxiv - Immunology 2019Quote: ... Transduced cells were selected for in the presence of 2 µg/ml puromycin (R&D Systems Inc, Minneapolis, MN, USA), live cells harvested by ficoll gradient and flow cytometry used to determine purity based on expression of eBFP indicative of transduction.
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Immunology 2019Quote: ... cover glasses were coated for 2 h at 37°C with 100 nM recombinant ICAM1-FC (796IC, R&D Systems) and washed 2 times with PBS ...
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bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
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bioRxiv - Bioengineering 2021Quote: ... Virus infection was detected with mouse mAb to SARS-CoV- 2 nucleocapsid (N) (R&D Systems, Minneapolis, MN, Cat#MAB10474) and counterstained with rabbit anti-mouse IgG-HRP (Rockland Immunochemicals ...
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bioRxiv - Developmental Biology 2022Quote: ... the differentiation media consisting of aRB27 with 2% Geltrex or Cultrex (+BME overlay) and 10ng/ml BMP4 (R&D Systems) was added to the cells [or with variations ...
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bioRxiv - Neuroscience 2023Quote: ... Culture media consists of N2B27 base medium supplemented with 20 ng/mL FGF-2 (R&D Systems, 233-FB-010), and 50 units/mL-50ug/mL Penicillin-Streptomycin (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... DIV12 hippocampal neurons were treated with either 2 ug/mL of recombinant Nptx2 (CF 7816-NP-050, R&D Systems) or BSA as control ...
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bioRxiv - Microbiology 2022Quote: ... and MIP-2 (CXCL2) concentrations were evaluated in the lung homogenate supernatants by DuoSet ELISA Development Systems (R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Immunology 2024Quote: ... and were kept overnight in complete RPMI media with 50 ng/ml of recombinant human IL-2 (R&D Systems). NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ and IL-2 production were measured using a quantitative ELISA per the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Pathology 2023Quote: ... The supernatant was analyzed using the mouse Lipocalin-2 enzyme-linked immunosorbent assay (ELISA) Duoset (DY1857-05, R&D Systems) according to the manufacturer’s instruction in serial dilutions.
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bioRxiv - Cancer Biology 2023Quote: ... The strained cell pellet was resuspended in >80% ice cold Cultrex growth factor reduced BME type 2 (R&D Systems) in PDAC organoid medium ...
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bioRxiv - Immunology 2023Quote: ... mouse TNF-alpha DuoSet ELISA (DY410) and DuoSet ELISA Ancillary Reagent Kit 2 (DY008) were purchased from R&D system.
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bioRxiv - Cell Biology 2023Quote: ... A feeding layer of 2 ml RPMI growth medium supplemented with 10 ng/ml PDGF-BB ligand (R&D Systems) and/or 0.01 uM siRNA (see below ...
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bioRxiv - Cancer Biology 2024Quote: ... The dissociated tissue pellet was resuspended in cold Cultrex Reduced Growth Factor BME type 2 (Cultrex BME) (R&D systems) and 25 mL drops per well were plated on pre-warmed 48-well plates ...
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...
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bioRxiv - Cell Biology 2019Quote: ... C2C12 and cardiac fibroblasts were serum starved for 24 h before adding medium containing 5 ng/ml recombinant TGFβ1 for 2 days (R&D Systems, Minneapolis ...
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 cells/ well in a 24 well plate were seeded on 10ug/ml fibronectin-coated cover slips (R&D systems) in 500ul 20ng/ml macrophage colony stimulating factor (MCSF ...
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bioRxiv - Immunology 2021Quote: ... In another experiment mice were treated intravenously 2 days post infection with 12.5 μg/kg recombinant murine IL-23 (1887-ML-010/CF, R&D Systems) for 3 consecutive days.
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bioRxiv - Cell Biology 2021Quote: Isolated cardiac fibroblasts at Passage (P)2 were treated with 10 ng/mL TGF-β1 (R&D Systems Cat #240-B) in Fibroblast Growth Medium 3 for four days to assess myofibroblast differentiation ...
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bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Fibers were incubated in plating medium consisting of 15% Fetal Bovine Serum (Hyclone) and 2 ng/ml bFGF (R&D Systems) in DMEM.
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bioRxiv - Immunology 2020Quote: ... Levels of cytokines in culture supernatants were assessed using mouse IL-2 and IFN-gamma DuoSet ELISA kits (R&D Systems), according to manufacturers’ instructions.
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bioRxiv - Neuroscience 2022Quote: ... Group 2 ligands were derived from human transcriptomic data and included: TGFβ2 (R&D Systems, Cat. 302-B2-002, 5ng/mL), NLGN1 (Sino Biological ...
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bioRxiv - Pathology 2022Quote: Antibody responses were measured against recombinant beta-SARS-CoV-2 B.1.351 spike protein (S) and its receptor binding domain (RBD) (10777-CV and 10735-CV, R&D Systems) by ELISA as previously described [23] ...
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bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed three times with PBST and incubated with 15 μl of DAB (3,3’-Diaminobenzidine) chromogen (2 ml of DAB chromogen (860001, R&D Systems) diluted in DAB chromogen buffer (860005 ...
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bioRxiv - Microbiology 2020Quote: ... Fecal lysates were analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2022Quote: ... were determined by the CXCL2/MIP-2 DuoSet enzyme linked immunosorbent assay (ELISA) kit to the manufacturer’s instructions (R&D Systems). Horseradish peroxidase-catalyzed color reaction were initiated by addition of the TMB Substrate Kit (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: Human or rhesus iPSCs were dissociated using Accutase and seeded onto Matrigel coated plates at a density of 50,000/cm2 in cold mTESR1 supplemented with 2% Cultrex (R&D Systems), 10 µM Y-27632 ...