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Citations for R&D Systems :
2751 - 2800 of 3214 citations for 8 1 Piperazinyl imidazo 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... followed by incubation with an anti-goat IgG horseradish peroxidase-conjugated secondary antibody at a 1:1000 dilution (HAF107; R&D Systems). As a control ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropili-1 inhibition studies were repeated as described above for EG00229 using a goat polyclonal anti-NRP-1 blocking antibody (AF566; R&D Systems) at 1 µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was thoroughly washed with PBS containing 0.05 % Tween-20 (0.05 % PBS-T) and then incubated for 1 h at room temperature with Anti-Mouse IgG HRP-conjugated antibodies (R&D Systems), diluted 1:1000 in the blocking buffer ...
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bioRxiv - Microbiology 2023Quote: The levels of mouse TNF-α and IL-1β were quantified from the supernatant of homogenised skin samples using Mouse TNF-alpha DuoSet ELISA (DY410) or Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401) (R&D Systems) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no. 39649, lot 02617020, used 1:250; SOX2, R&D Systems, AF2018 ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine levels in BMEF were then measured using mouse CXCL12/SDF-1 alpha (MCX120) and SCF (MCK00) Quantikine ELISA kits (R&D Systems) according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used in this study: Goat IgG against VE-cadherin (1:100, AF1002, R&D Systems, Minneapolis, MN), pY658 and pY685 VEC (1:40 ...
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... membranes were incubated with horseradish peroxidase⍰conjugated secondary antibody (hCD55 and hCD39, rabbit IgG, 1:5000 (Cat. no. HaF008, R&D Systems); hTBM and β-actin ...
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bioRxiv - Immunology 2024Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of protein from mock and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... or various doses (100 ng/mL, 200 ng/mL, 400 ng/mL) of recombinant mouse IGF-1 (catalog #: 791-MG-050; R&D Systems) for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems).
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies at 4 °C overnight (14-20 h) at the following dilutions: anti-PDGFRα (AF1062-SP, goat IgG, 1:250; R&D Systems), anti-APC (Ab-7 ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary incubation was 1 h at ambient temperature in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, MN ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910), or MCP-1 (MAB679, clone 23007) obtained from R&D Systems. For patient sample analysis using the immunostorm chip 10 µL of diluted human plasma (1:10 dilution with 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-6 (MAB9540, clone 973132), IL-12p70 (MAB611R, clone 24945R), and MCP-1 (AF-279, polyclonal) obtained from R&D Systems. Surface enhanced Raman scattering (SERS ...
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bioRxiv - Immunology 2024Quote: ... After overnight incubation (16-18h), cells were treated with vehicle (PBS + 1% BSA) or rmAreg (200, 500, or 1000 ng/ml) (R&D Systems). After 4h of incubation ...
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bioRxiv - Cell Biology 2020Quote: ... during 2 hours and stimulated with human recombinant VEGF165 (25ng/ml, R&D System) during 30 minutes.
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bioRxiv - Immunology 2021Quote: ... and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems) were performed according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... NK cells were activated for 72h in 300IU/ml rhIL-2 (R&D Systems). CD4 T cells were simultaneously activated and infected as described above ...
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bioRxiv - Bioengineering 2019Quote: ... 0.004 % of recombinant human FGF basic (FGF-2, R&D Systems, Abingdon, United Kingdom) was added ...
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bioRxiv - Bioengineering 2020Quote: ... and recombinant human IL-2 (50 U/mL, R&D Systems, Minneapolis, MN, USA). Single cell suspensions were activated with 1 μg/ml Concavalin A (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% 2-Mercaptoethanol and 10 ng/mL basic FGF (R&D Systems, 233-FB). For MyoD+F/R/C condition ...
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bioRxiv - Bioengineering 2021Quote: ... 100M PBMCs were thawed in 100 mL X-VIVO™ 10 media from Lonza (#04-380Q) with recombinant human IL-2 protein from R&D Systems (#202-IL). After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems). For the TGFβ2 assay ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 30 U/mL recombinant human IL-2 (R&D Systems #202-IL), and 55 μM beta- mercaptoethanol (Thermo # 21985023) ...
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bioRxiv - Developmental Biology 2019Quote: ... After 2 days EBs were treated with 100 ng/ml Noggin (R&D Systems) and 10 mM SB431542 (Tocris ...
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bioRxiv - Immunology 2019Quote: ... IL-1β (PBMC: 2 ng/ml, whole blood: 20 ng/ml, R&D Systems), (5 ...
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bioRxiv - Immunology 2019Quote: ... overnight before addition of 500 IU/ml recombinant human IL-2 (R&D Systems).
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bioRxiv - Immunology 2019Quote: ... MN) in combination with hrIFNβ at 2 ng/ml (R&D Systems, Minneapolis, MN) or when indicated hrISG15 at 0.115 μg/ml (Biotechne ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human MMP-2 (902-MP-010) was purchased from R&D systems (USA). Recombinant human MMP-3 (SRP7783 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems). For the TGFβ2 assay ...
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bioRxiv - Pathology 2021Quote: ... Samples were assayed into a Human ACE-2 DuoSet ELISA assay (R&D systems), loading equal amounts of protein in duplicate for all samples.
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bioRxiv - Immunology 2021Quote: ... Recombinant SARS-CoV-2 Spike glycoprotein was purchased from R&D Systems (Minneapolis, MN). Bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 B.1.1.529 S GCN4-IZ-2P (11061-CV, R&D Systems), MERS-CoV S GCN4-IZ-2P (R&D Systems) ...
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bioRxiv - Bioengineering 2022Quote: ... or 150 ng/ml recombinant human LOX homolog like 2 (LOXL2, R&D Systems), with media changed every 2-3 days ...
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bioRxiv - Immunology 2022Quote: ... 200 IU/ml of recombinant human IL-2 (R&D Systems, 202-IL-050) and expanded for 10 – 14 days ...
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bioRxiv - Neuroscience 2022Quote: ... and 100 ng/mL OX-2 membrane glycoprotein (CD200) (R&D systems, cat.no. 27712CD050) growth factors in addition to the previously described growth factors.
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bioRxiv - Bioengineering 2023Quote: ... 10 μM Y-27632 and 2 ng/mL human recombinant BMP4 (R&D Systems) and then cultured in ultra-low attachment 6 well plate (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... Media was supplemented with 2% fetal bovine serum (FBS) (R&D Systems, Minneapolis, MN), 1% penicillin-streptomycin 10,000U/mL (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... we used the Mouse IL-2 DuoSet ELISA kit (R&D Systems; DY402-05) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 20 ng/mL of recombinant mouse interleukin (IL)- 2 (R&D systems), 8% CTS Immune Cell Serum Replacement (Thermo Fisher) ...
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Low-dose cytokine immunotherapy of solid cancers enabled by phagocytic-competent protein co-crystalsbioRxiv - Cancer Biology 2023Quote: ... 3 and 6 days and subsequently tested by IL-2 ELISA (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... purified sheep IgG (2 mg/mL for LRIG1 R&D Systems #5-001-A), and mouse IgG1-PE conjugate (10 uL for LGR5 ...
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bioRxiv - Neuroscience 2024Quote: ... and proximal colon and plasma lipocalin 2 (LCN2) (#MLCN20, R&D Systems Minneapolis, MN) were also performed according with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and 100 μM of dynole31-2 (negative control) (R&D Systems, Minneapolis, MN, USA) before or after illumination ...