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Citations for R&D Systems :
251 - 300 of 328 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... basal media samples were collected for quantification of soluble CCSP via a premade Magnetic Luminex Performance Assay kit (Uteroglobin/SCGB1A1, R&D Systems). Culture media was collected from the bottom chamber of each tissue device and immediately frozen at −20°C until use ...
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bioRxiv - Cell Biology 2020Quote: ... An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Supernatant samples were collected and pre-diluted 5-fold before quantification of cytokines by ELISA assays targeting IL-1β (R&D systems) or IL-18 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal homogenate samples were used to determine NRG1 levels with the Human NRG1-β1 ELISA (DY377, R&D Systems, Minneapolis MN, USA). Samples were diluted in kit assay buffer (1:6 dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: All samples were assessed by direct-sandwich enzyme-linked immunosorbent assay (ELISA) under the manufacturer’s (R&D System Europe Ltd, Abingdon, UK) according to the National Institute for Biological Standards and Controls (Blanche Lane ...
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bioRxiv - Cell Biology 2020Quote: Plasma samples from 22 ZZ-AATD and 21 non-AATD controls were analyzed for CX3CL1/ Fractalkine using Duoset kit (R&D systems, Minneapolis ...
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bioRxiv - Microbiology 2022Quote: ... then stained with 0.1 µg/sample Alexa Fluor 647-conjugated mouse-anti SARS-CoV-2 Spike S1 (R&D Systems, FAB105403R-100UG) at 4°C for 30 min in the dark ...
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bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... For TGF-β1 detection in condition media from HPC treated with different experimental conditions and in urine samples from mice and human were analyzed by TGF-β1 ELISA commercial kit (R&D Systems) according to the man-ufacturer’s protocol.
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... The following antibodies were purchased and used for immunostaining of human sample using staining protocols similar to those used in mouse sections: CD31 (AF806, R&D systems), CXCR4 (2B11 ...
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bioRxiv - Cancer Biology 2020Quote: Human S100A8/S100A9 heterodimer amounts in human plasma samples were quantified using S100A8/S100A9 heterodimer Quantikine ELISA kit (R&D System, DS8900) following the manufacture protocol ...
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bioRxiv - Microbiology 2023Quote: The IL-1β levels in supernatant samples were measured using the Mouse IL-1β/IL-1F2 ELISA kit (R&D Systems, DY401), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... sterile filtered and used in a Milliplex assay to measure cytokines and chemokines in each sample using a 5- and 25plex customized kit (Millipore and R&D Systems). Data were analyzed by calculating the average value of the biological duplicates and then the ratio of Mtb treated / control ...
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bioRxiv - Immunology 2023Quote: ... Unpurified transient transfection supernatant samples (50 µl) were pre-incubated with biotinylated SARS-CoV-2 spike protein (R&D Systems cat# BT10549) (50 µl ...
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bioRxiv - Immunology 2024Quote: ... Total THP levels of a subset of urine samples (diluted 1:10) was determined by ELISA (catalog no. DY5144-05, R&D Systems) per manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... of medium samples were analysed by SDS-PAGE under reducing conditions and underwent Western blotting using anti-Wnt5a antibody (MAB6452, R&D systems). Immune signals for exogenously added Wnt5a in the medium and cell lysate were quantified using ImageJ ...
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bioRxiv - Cell Biology 2023Quote: The amount of VE-Cad and Rad51 protein in the conditioned media (CM) of PMVECs or human and rat plasma samples containing EDTA was quantitated by using ELISA kit (DCADV0, R&D systems for VE-Cad and custom made by MyBiosource for Rad51 ...
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bioRxiv - Microbiology 2023Quote: The levels of mouse TNF-α and IL-1β were quantified from the supernatant of homogenised skin samples using Mouse TNF-alpha DuoSet ELISA (DY410) or Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401) (R&D Systems) following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... and apurinic or apyrimidinic (AP or abasic) sites in the samples were performed using HT 8-oxo-dG ELISA Kit (R&D Systems) and AP Sites Quantitation Kit (Cell Biolabs) ...
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bioRxiv - Cancer Biology 2022Quote: Human cytokine secretion was assessed in mouse plasma samples using the Human XL Cytokine Luminex Performance Panel Premixed Kit (#FCSTM18, R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... The samples were further processed following the Human Phospho-Receptor Tyrosine Kinase Kit (R&D Systems Europe, Ltd., Abingdon OX14 3NB, UK) manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine serum samples were analyzed using an R&D systems Murine Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN, USA) and a Luminex 200 (RRID ...
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bioRxiv - Cancer Biology 2022Quote: Freshly sorted m-LSCs from primary AMLs or prepared CD34+ HSPCs isolated from normal mobilized peripheral samples were plated in human methylcellulose (R&D systems) at about 100K/ml and 2K/ml ...
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bioRxiv - Physiology 2023Quote: Circulating TNFR1 and TNFR2 were measured in human or mouse samples using enzyme-linked immunosorbent assay (ELISA) kits (DY255, DY726, DY425 and DY426 respectively, all from R&D Systems) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: The concentration of ELANE in the patient plasma samples was measured using a Human Neutrophil Elastase/ELA2 DuoSet ELISA kit (DY9167, R&D Systems). ELANE expression in mouse corpus callosum areas and plasma samples was measured using commercial ELISA kits (MELA20 ...
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bioRxiv - Cancer Biology 2024Quote: ... Each human plasma sample was loaded independently on a membrane of the Proteome Profiler Human XL Cytokine Array (ARY022B, R&D systems) following the manufacturer’s recommendations and background noise removal was performed identically.
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bioRxiv - Biochemistry 2024Quote: ... and manufacturer’s kit instructions were followed for analysis using undiluted samples (Mouse S100A8/S100A9 Heterodimer DuoSet ELISA #DY8596-05 and DuoSet Ancillary Reagent Kit #DY008B, R&D Systems). Results were reported as calprotectin (pg)/total fecal protein (µg).
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bioRxiv - Cell Biology 2021Quote: ... intracellular staining was done using the permeabilization buffer in the FIX & PERM kit with PE-conjugated anti-mouse α-SMA (clone: 1A4, R&D Systems) at 1:20 in the permeabilization buffer ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and stained with goat anti-ACE2 (R&D Systems, #AF933) at a 1:50 dilution or rabbit anti-LRRC15 (abcam ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 5 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of IL-1β conjugate (R&D Systems, UK). Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of detection antibody (R&D Systems, UK). Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP ...
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bioRxiv - Immunology 2020Quote: ... cells were pelleted and resuspended in 100 µL FACS buffer (2% FBS in DPBS) containing 0.2 µg goat-α-human Ace-2 (R&D Systems #AF933). After 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... paraformaldehyde-fixed frozen sections were treated with citrate buffer (pH 7.0, 121°C, 5 min) before immunostaining with sheep anti-mouse Spi-B polyclonal Ab (R&D Systems, Abingdon, UK). CCL20 ...
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bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed twice with cold FACS buffer and consequently incubated with anti-mouse F(ab)2 IgG (H+L) PE-conjugated antibody (R&D systems, F0102B) for 1h in the dark at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... was used after viability and extracellular staining and cells were incubated in Perm/Wash buffer with Arginase-1 (APC, R&D systems #IC5868A) for 30 minutes at 4℃ ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then incubated with the first primary antibody (an anti-IL-1R1 antibody, 1: 100, MAB269, R&D Systems, Minneapolis, USA). Negative controls were incubated in the same solution without the primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... 100 µl of supernatant samples and standards (recombinant mouse TREM2-FC, 1729-T2; R&D Systems; 0-20 ng/ml in BSA-PBST) were then incubated for 2 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were blocked in 2% goat serum for 45 mins at room temperature and incubated with anti-Snail (R&D Systems, Minneapolis, MN), anti-Zeb1 (Invitrogen ...
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bioRxiv - Pathology 2020Quote: ... IGF-1 was measured in the samples using a R&D Systems Mouse 1-Plex Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Apical washes and basolateral media samples were clarified by centrifugation and analyzed using the Magnetic Luminex® Human Discovery Assay (R&D Systems). Custom configured targets include ...
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bioRxiv - Physiology 2023Quote: The concentration of osteopontin in human milk samples was determined in triplicate using the Human Osteopontin ELISA Kit (R&D Systems, Minneapolis, MN). Samples were diluted 1:100,000 in the provided buffer and analyzed following the manufacturer’s instructions.
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bioRxiv - Pathology 2023Quote: ... We quantified OCN content of the EDTA-solubilized samples by an enzyme-linked immunosorbent assay (Bio-Techne R&D Systems, Minneapolis, MN, USA). OCN content was normalized to protein content and expressed as ng OCN/mg protein.
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bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
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bioRxiv - Pathology 2023Quote: All mouse serum samples were analyzed by a custom R&D systems Murine Premixed Multi- Analyte Kit (R&D Systems, Inc., Minneapolis, MN). The panel was run on a Luminex 200 Instrument (Luminex Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then subjected to western blot analysis detailed above to detect TAOK2 autophosphorylation by rabbit antibody against pS181 (R&D Systems, PPS037).
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bioRxiv - Immunology 2023Quote: Levels of cytokines and chemokines were assessed in serum samples by sandwich ELISA using commercial kits (DuoSet R&D Systems, Minneapolis, MN, USA) following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: TNF-α and IL-6 in BAL fluids and serum samples were measured using ELISA kit (TNF-α: MTA00B; IL-6: M6000B-1) from R&D Systems, according to the recommended protocols from the manufacturer.
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bioRxiv - Neuroscience 2024Quote: Phosphorylation levels were measured in remaining hDRG protein samples using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems, CAT# ARY003C). The commercial protocol was followed ...
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bioRxiv - Pathology 2024Quote: ... Urine samples were stored at −20°C and when ready were processed using a Creatinine Parameter Assay kit (R&D systems, cat# KGE005) and a mouse Albumin ELISA kit (Bethyl Laboratories ...