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Citations for R&D Systems :
101 - 150 of 328 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... Samples were assayed into a Human ACE-2 DuoSet ELISA assay (R&D systems), loading equal amounts of protein in duplicate for all samples.
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bioRxiv - Cell Biology 2022Quote: ... the samples were treated with human recombinant LOXL3 (RhLOXL3, R&D systems, 6069-AO) or LOXL2 (RhLOXL2 ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated anti rat IgG for mouse samples (R&D Systems Cat#VC005-125), Rabbit Linker (Agilent Cat#GV80911-2) ...
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bioRxiv - Molecular Biology 2024Quote: ... Stool samples were analyzed for Lipocalin-2 levels using ELISA (R&D Systems, #DY1857).
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bioRxiv - Immunology 2024Quote: ... Samples were then probed with antibodies specific for IL-1𝛼 (R&D Systems MAB200), IL-1β (R&D Systems MAB201) ...
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bioRxiv - Cell Biology 2024Quote: ... Standard curve samples were prepared by diluting recombinant human GALC protein (R&D Systems) in culture supernatant from KO cells to 0.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Additional reagents that were used are 2N Sulfuric Acid Stop Solution (Catalog no. DY994, R&D Systems, Minneapolis, MN), Triphenylphosphine (TPP ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of a solution containing 0.001 mg/mL of the detection biotinylated polyclonal antibody (BAF1559; R&D Systems) raised against NOTCH3 ECD and 2.5% BSA was added and was used for detection followed by agitation for 2h at RT ...
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bioRxiv - Synthetic Biology 2024Quote: ... CHO-derived rhBMP2 standard and streptavidin-HRP working solution from the hBMP2 DuoSet ELISA system (DY355, R&D systems) were used for all experiments ...
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bioRxiv - Immunology 2024Quote: ... and anti-B220 (Rat anti-mouse; monoclonal: RA3-6B2 clone; dilution 1:100 in blocking solution; R&D systems). Secondary antibody staining was performed for 50min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Colonoids were passaged every 10-14 days by harvesting in Cultrex Organoid Harvesting Solution (R&D Systems, Minneapolis, MN) at 4°C with shaking for 30’ ...
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bioRxiv - Cancer Biology 2023Quote: ... in 2% BSA/PBS solution and the appropriate fluorescently-conjugated secondary antibody (R&D Systems catalog #NL006, 1:200). Coverslips were mounted onto slide with DAPI-containing ProLong Gold media ...
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bioRxiv - Cancer Biology 2024Quote: ... Each microwell was then overlaid with 2 μL of organoid digestion mix including Organoid Harvesting Solution (R&D Systems) with 10 mg/mL B ...
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bioRxiv - Immunology 2024Quote: ... were suspended in 200 μL of a 125 nM solution of recombinant human SIRPα-Fc protein (R&D Systems), the cognate isotype control recombinant human IgG1 protein (Sino Biological) ...
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bioRxiv - Physiology 2021Quote: ... incubation buffer and TB4 antibody prediluted at 1:4000 (AF6796; R&D Systems, Minneapolis, MN,) were added to sterile borosilicate tubes and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Secreted IFN-β in samples were compared to a purified IFN-β (R&D Systems) standard curve using a BioTek EL312e microplate reader (450 nm).
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bioRxiv - Developmental Biology 2022Quote: ... Samples were diluted at 2 μg/ml using Calibrator Diluent RD5-18 (R&D Systems). Hyaluronan quantification was then performed using the Hyaluronan Quantikine™ ELISA kit from R&D Systems (DHYAL0).
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bioRxiv - Cancer Biology 2022Quote: ... SDF-1α levels were determined in duplicate for each sample by ELISA (R&D Systems). Optical density was measured at 450 nm and 570 nm (correction ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were then incubated with primary antibodies against PDGFRα (Goat IgG, R&D systems AF1062), Collagen I (Rabbit IgG ...
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bioRxiv - Microbiology 2021Quote: ... according to manufacturer’s instructions and utilizing a Sample Activation Kit 1 (R&D Systems #DY010) and Human TGF-β1 Controls (R&D Systems #QC01-1) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then analyzed through immunoblotting probing against TYRO3 (1:1000, R&D Systems, MAB859100), TIM (TIM (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... whole blood samples were incubated with biotinylated antibodies against CD45 (R&D Systems, clone 2D1), CD66b (AbD Serotec ...
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bioRxiv - Cancer Biology 2023Quote: ... ELISA assays on frozen human serum samples were performed using Luminex Assay (R&D Systems) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used overnight in 1% skimmed milk solution: anti-UCP1 (1:750, R&D Systems, MAB6158), anti-pCREB (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Drugs used for flow cytometry experiments were QVD-Oph (#OPH001, R&D Systems, 10 mmol/L stock solution in DMSO), S63-845 (Chemieteck ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed by lysis buffer provided in the kit (R&D Systems, #895943). The supernatant was collected after centrifugation at 14,000xg for 5 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 100 nM HsUBA1 (E1) and 10 mM ATP in ubiquitin conjugation reaction buffer (R&D systems).
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bioRxiv - Cell Biology 2024Quote: ... and the reaction buffer components of the Ubiquitin conjugation initiation kit (K-995, R&D systems) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag mouse monoclonal antibody (MAB050) and the basic-based antigen unmasking solution (CTS013) from R&D Systems (Minneapolis, MN); anti-FLAG M2 mouse monoclonal antibody (F1804) ...
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bioRxiv - Immunology 2021Quote: ... In-Cell ELISA was then developed using DY999 substrate solution according to the manufacturer’s recommendations (R&D Systems, Minneapolis, MN USA) and measured at 450 nm (and at 630 nm to assess the background ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were resuspended in an antibody solution containing 5μL DYKDDDDK Epitope Tag Alexa Fluor 647-conjugated Antibody (R&D Systems #IC8529R) and 45μL DBPS + 10% FBS and incubated in the dark at room temperature for one hour ...
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bioRxiv - Molecular Biology 2023Quote: ... were prepared to obtain solutions of TGF-β1 (10 ng/mL and 25 ng/mL, R&D Systems 240-B-002), palmitic acid (PA ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were immuno-precipitated with 2-5 µg of either anti-GFI1 (AF3540, R&D systems), anti-H3K4me1 (ab8895 ...
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bioRxiv - Neuroscience 2021Quote: Plasma samples were assayed for FGF-21 levels using an ELISA kit from R&D Systems (MF2100 ...
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bioRxiv - Physiology 2020Quote: ... samples were analyzed according to manufacturer instructions using the Phospho-MAPK Array (R&D Systems, #893909). For western blots ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were treated with recombinant human PD-1 Fc protein (1086-PD-050, R&D System) at 4 °C overnight ...
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bioRxiv - Pathology 2020Quote: ... An anti-TLR4 capture antibody (100 ng/well in carbonate-bicarbonate buffer; R&D Systems, Minneapolis, MN) with demonstrable specificity for TLR in vitro (see Western blot ...
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bioRxiv - Immunology 2022Quote: ... and all steps were followed by washing using 200 μL/well of wash buffer (R&D Systems) three times ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with primary antibody buffer mix consisting of gtPDGFRß (1:100; AF1042, R&D Systems), rbCollagen IV (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min each time and subsequently incubated with blocking buffer (5% goat serum [R&D Systems, Minneapolis ...
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bioRxiv - Cancer Biology 2021Quote: ... one ml solution of 250 ng/ml type I collagen with or without 250 ng/ml POSTN (R&D Systems, Minneapolis, MN) was added to each well followed by one-hour incubation at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked with a 10% Bovine Serum Albumin solution in PBS for 20 minutes and then incubated with the Alexafluor 647-conjugated antibody for Human ACE-2 (R&D Systems). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40; AF3075; R&D Systems) and rabbit polyclonal antibodies against GFAP (1:250 ...
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bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
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bioRxiv - Developmental Biology 2024Quote: ... Organoids were retrieved from Cultrex by removing culture media and adding 500uL Cultrex Organoid Harvesting Solution (R&D Systems; 3700-100-01) to the wells of a 48 well plate ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels (n=3) were incubated for 4 hours in a solution of FBS-free DMEM supplemented with 10% Resazurin dye (AR002, R&D Systems) at 37°C ...
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bioRxiv - Bioengineering 2024Quote: The cerebral organoids in Matrigel were isolated from the pillar plate using Cultrex organoid harvesting solution (3700-100-01, R&D Systems) according to the manufacturer’s recommended protocol ...
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bioRxiv - Bioengineering 2023Quote: The cerebral organoids in Matrigel were isolated from the pillar plate using Cultrex organoid harvesting solution (3700-100-01, R&D Systems) according to the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2023Quote: E13.5 small intestinal tissues were harvested in Calcium/Magnesium free (CMF) PBS and washed several times in CMF PBS before being placed in 3mL of Cultrex Organoid Harvesting Solution (R&D Systems), a non-enzymatic basement membrane depolymerization solution ...