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Citations for R&D Systems :
51 - 100 of 328 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... were embedded in a Bmp7 solution (R&D systems, Recombinant Human BMP7 #354-BP) in PBS ...
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bioRxiv - Physiology 2023Quote: ... before plates were incubated with 3,3′,5,5′-tetramethylbenzidine (TMB) substrate solution (R&D Systems). Enzymatic reactions were quantified by measuring absorbance at 450 nm using a standard plate reader (Biotek Instrument).
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bioRxiv - Neuroscience 2023Quote: cleaved caspase 3 (Cat. No. MAB835, 1:200 in blocking solution, R&D Systems), dsDNA (Cat No ...
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bioRxiv - Biochemistry 2024Quote: ... All samples were deglycosylated with PNGase F (R&D Systems) for 16h at 37°C.
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bioRxiv - Bioengineering 2024Quote: ... Samples were subsequently treated with primary (R&D Systems; AF1997) and secondary (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with lysis buffer 17 (R&D Systems, 895943) and protein quantified using bicinchoninic acid protein assay (BCA ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10x E3 Ligase Reaction Buffer (R&D systems B-71). Addition of Rik1-TAP complex purified from S ...
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bioRxiv - Physiology 2022Quote: ... Cells were thereafter lysed with Lysis Buffer #6 (R&D Systems) and assayed for the expression levels of phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187 ...
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bioRxiv - Cancer Biology 2023Quote: ... homogenized and digested in Cell Lysis Buffer #2 (R&D Systems) containing protease inhibitors on ice for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... SK1 reaction buffer contained 100nM SK1 (R&D systems, Minneapolis, Minnesota), 25uM NBD-Sph (Cayman Chemical Company ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed with lysis buffer 17 (R&D Systems; 895943) supplemented with protease cocktail (Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... was mixed with coating solution (1X; 100μL; cat. no. 3455-096-03, R&D systems) was placed into Falcon™ Cell Culture Inserts (0.4 μm pore size ...
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bioRxiv - Neuroscience 2023Quote: A solution of 10 ng interferon-β (IFN-β, R&D Systems, 8234-MB-010) diluted in PBS (2μl ...
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bioRxiv - Developmental Biology 2024Quote: ... 50 µl solution of FGF8 recombinant mouse protein (424-FC-025/CF, R&D systems) at a concentration of 1 µg/ml was dropped by a pipette on the ventral surface of the embryo.
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bioRxiv - Immunology 2022Quote: ... 50 μL/well of a 1:1 solution of H2O2 (R&D Systems, part #895000) and tetramethylbenzidine (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... the samples and standards (Trem2, R&D Systems, 1729-T2-050) were incubated for 2 hrs at RT with shaking ...
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bioRxiv - Cancer Biology 2023Quote: The caspase inhibitor sample pack (R&D systems-Cat No FMKAP01); which contained Z-VAD (general caspase inhibitor) ...
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bioRxiv - Genetics 2020Quote: Cells were washed with Flow Cytometry Staining Buffer (FC001, R&D Systems) and then stained in 50ul of Staining Buffer for one hour on ice with the following conjugated antibodies multiplexed at the indicated dilutions ...
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bioRxiv - Cancer Biology 2021Quote: Cells were lysed in ice-cold Lysis Buffer 6 (R&D systems) supplemented with 10 μL/mL of protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with FACS buffer (R&D Systems, Minneapolis, MN, USA) thrice and centrifuged at 300 ×g and 4 °C for 5 min ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Sections were first incubated with an antigen retrieval solution (Antigen Retrieval Reagent-Basic, R&D Systems) at 80°C for 30 minutes followed by blocking (2% non-fat dry milk ...
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bioRxiv - Bioengineering 2021Quote: ... the slides were incubated in 5 μg/mL solution of anti-SOX2 antibodies (R&D Systems) or 5 μg/mL solution of anti-Sox17 (SantaCruz ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% BSA-containing DBPS in Cultrex rat Collagen I solution (R&D Systems, 3440-005-01) 170 ul was loaded in the center chamber ...
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bioRxiv - Bioengineering 2023Quote: ... Pcsk9 from the plasma samples were analyzed by ELISA (R&D systems) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: For analysis of murine plasma samples an OPN (#DY441, R&D Systems), a neutrophil elastase (ELA2 ...
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bioRxiv - Immunology 2022Quote: ... Lastly the samples were incubated with IL-15-AlexaFluor700 (R&D Systems), Ccl19-AlexaFluor488 (Bioss ...
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bioRxiv - Immunology 2023Quote: ... Samples were analysed using the human IL-33 duoset (R&D Systems) and the IL33004-IL330425-biotin assays (AstraZeneca) ...
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bioRxiv - Immunology 2021Quote: ... Cells were sequentially challenged by addition of the following agonist solutions: SLIGRL (100 μM; R&D Systems), then either rmIL-31 (300 nM ...
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bioRxiv - Developmental Biology 2022Quote: ... every 7 days Matrigel was lysed with Cultrex™ Organoid Harvesting Solution (R&D Systems™, MN), and isolated mGO ...
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bioRxiv - Developmental Biology 2023Quote: ... heating at 95°C for 5 min in antigen retrieval solution (CTS045, R&D Systems, Minneapolis, MN). After blocking non-specific binding in 2% non-fat milk in 0.1 M PBS containing 0.1% Triton X-100 (PBS/MT ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were solubilized using lysis buffer provided by the vendor (R&D Systems) and rocked for 30 minutes at 4°C ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... cell lysates were prepared by homogenization in lysis buffer 16 (R&D systems) and incubated on ice for 30 min ...
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bioRxiv - Immunology 2022Quote: ... The membranes were washed three times with 1× Wash Buffer (R&D Systems) to remove unbound proteins ...
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bioRxiv - Neuroscience 2024Quote: ... An additional 250 µL of Lysis Buffer 17 (R&D Systems, Minneapolis, MN) and 2.5 µL of 100X Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... and buffered with 1X E3 Ligase Reaction Buffer (R&D systems, B-71). Substrates and Z-VAD-FMK (UBPBio ...
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bioRxiv - Bioengineering 2021Quote: ... the medium was treated with Sample Activation Kit (DY010; R&D Systems™). TGF-β1 and GDF-15 ELISAs were performed using Substrate Reagent Pack (DY999 ...
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bioRxiv - Cell Biology 2021Quote: ... all samples were diluted 1:12 in reagent diluent (R&D systems, DY995). 50 pg/mL MMP1 was detected in 2% human serum and this value was subtracted from all serum-treated samples.
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bioRxiv - Immunology 2020Quote: ... Samples were analyzed with a mouse XL cytokine array kit (R&D Systems), according to the manufacturer’s instructions [49] ...
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bioRxiv - Cell Biology 2023Quote: ... samples were subjected to immunoblotting with sheep anti-DGKε antibody (R&D Systems) (Supplemental Table S2).
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bioRxiv - Developmental Biology 2024Quote: ... Each sample was then incubated with anti-Wnt5a antibody (AF645, R&D systems) and anti-LRP1 antibody (ab92544 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were probed with mouse anti-Nitrotyrosine (R&D Systems, MAB3248, 1:20) or mouse anti-Malondialdehyde (Thermo Fisher Sci ...
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bioRxiv - Immunology 2024Quote: ... the samples were developed with 3,3′,5,5′-tetramethylbenzidine (TMB; R&D Systems, #DY999) solution ...
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bioRxiv - Immunology 2024Quote: ... Samples were also evaluated for S100A9 concentration by ELISA (R&D Systems DY5578). All cytokines ...
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bioRxiv - Neuroscience 2024Quote: ... 50µL of lavage sample or standard (R&D systems, 406-ML-005/CF) was added to the blocked wells for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The lysed LVV solution was assessed using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems) as described previously76 ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 µg mL-1 biotin Polyclonal Goat anti-Fibroblast Growth Factor IgG solution (R&D Systems, USA), for the generation of anti-FGF-2 functionalized beads ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg mL-1 biotin Polyclonal Goat anti-Vascular Endothelial Growth Factor IgG solution (R&D Systems, USA), for the generation of anti-VEGF functionalized beads ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 hour with blocking buffer (Reagent Diluent, R&D Systems, UK). Plates were washed 3 times with wash buffer and 100 ul of samples and standards were added (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in Flow Cytometry Staining Buffer (R&D Systems, Minneapolis, MN, USA) and were analyzed using a BD Biosciences LSR II (RRID ...
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bioRxiv - Genomics 2020Quote: ... one sample was stained with mouse IgG isotype control (clone 11711, R&D Systems) diluted 1:5 in FACS buffer ...