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Citations for R&D Systems :
151 - 200 of 328 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Culture media on the top of the matrix was removed and washed in cold PBS and the matrix was depolymerized with 200ul of Cultrex organoid harvesting solution (R&D systems) at 4°C for 2 h and then the isolated cell spheroids were lysed with 1% NP-40 cell lysis buffer containing protease and phosphatase inhibitor cocktail (Thermo-Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... cells from each well were harvested and resuspended in 100 µL of cell resuspension solution provided with human methylcellulose enriched media (R&D systems). Each cell suspension was added to 2.5 mL methylcellulose enriched media ...
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs from each AggreWell were transferred to an Anti-Adherence Rinsing Solution treated well of a 12-well plate in media with 10 ng/ml BMP4 (R&D Systems), 5 ng/ml FGF2 (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... The non-biotinylated solution analyte proteins used in binding assays were either H1 protein or IGF2R domains 11-13 (R&D Systems), as applicable ...
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bioRxiv - Bioengineering 2024Quote: ... Brain organoids embedded in Matrigel on the 36PillarPlate were isolated using the Cultrex organoid harvesting solution (R&D Systems, 3700-100-01), which facilitates the non-enzymatic depolymerization of Matrigel ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were run in duplicates using the Mouse Magnetic Luminex Assay for IGF-1 (R&D System) and Milliplex Mouse Pituitary Magnetic Assay for GH (Merck ...
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bioRxiv - Cell Biology 2021Quote: ... and TNF-α) were quantified in the supernatants from hiPSC-CMs samples by ELISA (R&D Systems) following manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... plasma samples were analyzed using Quantikine ELISA Mouse GDF15 Immunoassay (ELISA, R&D systems, catalog no. MGD150). The ELISA assays were used according to the protocol provided by the manufacturer.
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bioRxiv - Cancer Biology 2022Quote: ... Samples were assayed immediately after using the Quantikine® Human TGF-β1 Immunosasay Kit (R&D Systems), the Human TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Physiology 2020Quote: ... plasma samples were analyzed using Quantikine ELISA Mouse GDF15 Immunoassay (ELISA, R&D systems, catalog no. MGD150). The ELISA assays were used according to the protocol provided by the manufacturer.
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bioRxiv - Cancer Biology 2020Quote: ... Supernatant samples (1 ml) and a Proteome Profiler Mouse XL Cytokine Array Kit (#ARY028, R&D Systems) were used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... for mouse samples or mouse anti-CD31 antibody (1:200, #BBA7, R&D systems, Minneapolis, MN, USA) for human samples ...
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bioRxiv - Bioengineering 2022Quote: Samples were diluted as needed and cytokine concentrations were determined via DuoSet ELISA Kits (R&D Systems): IL-6 (DY406) ...
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bioRxiv - Immunology 2024Quote: ... samples were pre-treated for 1h at 37°C with 0.1 mM TAPI-2104 (R&D Systems) prior to subsequent incubation with gp33 peptide as detailed above in the continuous presence of TAPI-2 ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated with goat anti-mouse CD45 antibody (1:50, AF114, R&D Systems, MN, USA) followed by horseradish peroxidase-conjugated rabbit anti-goat IgG (1:250 ...
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bioRxiv - Physiology 2023Quote: ... plasma samples were analyzed using Quantikine ELISA Mouse GDF15 Immunoassay (ELISA, R&D systems, catalog no. MGD150). The ELISA assays were used according to the protocol provided by the manufacturer.
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bioRxiv - Developmental Biology 2023Quote: ... the samples were incubated with the primary antibodies against TAG1 (1/500, R&D Systems, lot #CDSO0219101) and or DsRed (1/1000 ...
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bioRxiv - Cancer Biology 2023Quote: Primary AML specimens or normal mobilized peripheral blood samples were plated in human methylcellulose (R&D systems) at cell concentrations indicated in respective figures ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse tumors were homogenized with a tissue homogenizer in 700μL of Cell Lysis Buffer 2 (R&D Systems) containing 1x Halt Protease Inhibitor Cocktail (ThermoFisher Scientific) ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... Cell pellets were lysed in 1ml lysis buffer from Human phospho-kinase array kit (R&D systems, ARY003C) supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Immunology 2022Quote: ... all necessary diluents and buffers were from the DuoSet® Ancillary Reagent Kit 2 (R&D Systems, #DY008). The assays were performed at RT unless otherwise noted ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets from the peritoneal lavage and bone marrow were lysed using Lysis 2 buffer (R&D systems), containing HALT protease inhibitors (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... PBS was removed and replaced with 200 µL of blocking solution (dPBS with 2% (v/v) BSA) containing α-TIM1(1:100, AF1750, R&D Systems) or α - AXL antibody (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... in culture medium with recombinant mouse TNF-α (10 ng/ml from a stock solution of 100 μg/ml made up in 0.2 % bovine serum albumin and sterile PBS, R&D systems, aa-80325) to stimulate release of inflammatory mediators (TNF-FLS ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were fixed in 4% paraformaldehyde solution as previously described.39 Membranes were immunoblotted for AXL (AF154 R&D Systems at 1:500), MerTK (ab52968 ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µL of a 5 µg mL-1 biotinylated polyclonal Goat anti-Vascular Endothelial Growth Factor IgG solution in PBS (R&D Systems, USA) was added to CellStudio substrates containing streptavidin coated microbeads patterns (500 nm diameter beads ...
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bioRxiv - Neuroscience 2021Quote: ... the samples were stained with TRA-1-60 conjugated FITC antibody (R&D system, FAB4770G, 1:50 dilution) or normal IgM conjugated FITC (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... TNFα and IL1-β were analysed from the stored samples using ELISA Kits (R&D systems, MN, USA).
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bioRxiv - Cancer Biology 2022Quote: Cytokines were analyzed from plasma samples using the Proteome Profiler Human Cytokine Array Kit (R&D Systems, ARY005B) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Whole blood serum samples were diluted 1:40 in 100 µL of reagent diluent (DY995; R&D Systems) and recombinant human NOTCH3 ECD (1559-NT-050 ...
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bioRxiv - Microbiology 2023Quote: ... and GM-CSF in the samples was measured using the Luminex Multiplex panel (# LXSAHM-04, R&D systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cytokine concentration from DENV infected patients’ serum samples were determined via Luminex Assay Human 80 (R&D System) following manufacturer′s instructions (Table 5).
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bioRxiv - Biophysics 2023Quote: ... The samples were then exposed to either 10 nM thrombospondin-1 (TSP-1) (3074-TH, R&D Systems) or HBSS for 10 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: All samples were stained with 1X GloLIVE Human TRA-1-60 NL493 Live Cell antibody (R&D Systems) in stem cell media for 30 minutes at 37C on days 7 ...
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bioRxiv - Immunology 2020Quote: ... and Alexa Fluor 594 labelled anti-human DAP10/HCST (R&D Systems, 1:500 v/v in blocking buffer) to stain for the cytoskeleton and DAP10 clusters respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were pelleted and then resuspended in 1 mL of Flow Cytometry Staining Buffer 1X (R&D Systems #FC001) for blocking ...
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bioRxiv - Biochemistry 2023Quote: ... the gel was incubated in 20 ml Buffer A supplemented with 20 μM Suc-LLVY-AMC (R&D Systems) at 30°C for 10 min and imaged under UV light ...
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bioRxiv - Cell Biology 2021Quote: ... or Recombinant Mouse Wnt-3a (10µg/ml stock solution in 0.1% BSA in PBS) (1324-WN-002, R&D systems, Bio-Techne, Minneapolis, MN) with DMSO and 0.1% BSA in PBS as vehicle controls ...
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bioRxiv - Microbiology 2021Quote: ... Domes were dislodged with a cell scraper in 1 mL of Cultrex Organoid Harvesting solution (Bio-techne, R&D Systems brand, 3700-100-01) and incubated for 1 hr at 4°C on a shaker at 250 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... The cannula was connected to an osmotic minipump filled either with vehicle solution (PBS containing 0.1% BSA as a carrier) or 35 μg/ml of soluble TNF receptor-1 (sTNFR1, R&D Systems Inc. Minneapolis, MN).
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bioRxiv - Cell Biology 2024Quote: ... isolated acinar clusters were resuspended in a stiff matrix composed by 1:1:1 mix of Matrigel-GF reduced : rat collagen I neutralized solution 4.43 ng/mL (R&D Systems – 3440-100-01) ...
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bioRxiv - Cancer Biology 2020Quote: ... ELISA’s were performed using 50 μl of media per sample and following the manufacturer’s protocol (R&D systems, DTFF30). After removal of media ...
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bioRxiv - Microbiology 2021Quote: ... was determined by ELISA in the plasma samples using a colorimetric assay kit (#DFBP20, R&D systems, Minneapolis, MN) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2020Quote: ... kidney samples were processed for TNF-α expression using the Mouse TNFα Quantikine ELISA Kit (R&D Systems MTA00B). Samples were diluted 1:5 in reagent diluent prior to plating ...
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bioRxiv - Immunology 2020Quote: Samples stored at −20C were analyzed using IL-5 and IL-13 ELISA kits (R&D Systems, Minneapolis, MN) as per the company’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 50μl of undiluted BMEC media samples and nitrate (NO3) standards ranging 100μM to 1.565μM were plated on clear polystyrene microplates (DY990; R&D Systems). To measure total nitrites (NO2 ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were serum-starved for 12 h and then treated with 100 ng/ml BMP2 (R&D Systems) for 3 h ...