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Citations for Santa Cruz :
4851 - 4900 of 5084 citations for Mouse Serine threonine protein kinase pim 2 PIM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Approximately 300 μg of cell lysate at 1-1.5 mg/ml was then pre-cleaned by rotation incubation with 20 μl 50% Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Texas, USA) at 4°C for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Molecular Biology 2022Quote: ... The remained liquids were added with anti-NS1 or -VP2 or -IgG antibodies and incubated at 4°C for 4 h followed by addition of protein A/G PLUS-Agarose (Santa Cruz Biotech, sc-2003) and incubation overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to each sample and then incubated at 4°C for 4 hours followed by addition of protein A/G PLUS -Agarose (Santa Cruz Biotech, sc-2003) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins extracts (1.5 mg) of embryos expressing CG11412-myc were incubated with 1 μg of c-Myc antibody (Santa Cruz, sc-40, clone 9E10) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... raised against aa112-608 of recombinant pORF2 protein as used in a previous study (Glitscher et al., 2021a)) as well as anti-GAPDH (Santa Cruz Biotechnology, 1:2,000) and anti-beta-Actin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... the HIS-tagged AtCFI25a protein bound to GST-AtCFI59 was detected by western blot analysis using an anti-HIS antibody (G18) (sc-804; Santa Cruz Biotechnology, Dallas, USA) at 1:5000 dilution.
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bioRxiv - Cell Biology 2023Quote: ... 50 µg of protein extract was incubated at 37°C for 1 hr with the artificial p-nitrocatecholsulfate substrate (Santa Cruz Biotechnology, sc-238927A) and either sodium pyrophosphate (ARSA ...
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bioRxiv - Microbiology 2023Quote: ... and primary antibodies to cellular proteins were used as markers for early endosomes (EEA-1; rabbit polyclonal antibody; Santa Cruz Biotechnology Inc; 1:200), and the trans-Golgi (TGN46 ...
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bioRxiv - Cell Biology 2023Quote: ... were immunoprecipitated by incubation with protein A/G magnetic beads (HY-K0202, MCE) and anti-GFP antibody (sc-9996, Santa Cruz Biotechnology; 1:50) at 4 °C overnight with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were loaded per lane and blots were probed with anti-mGFP antibodies (Santa Cruz Biotech, #sc-9996; 1:2000 dilution), anti-elongation factor 1-α (EF1A ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were incubated with primary antibody or rabbit IgG (control) for 3hr at 4°C and then with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, sc-2003) beads for 35min with head-to-tail rotation either in the absence or presence of 100 µM CCT blocking peptide SAGRRFIVSPVPE (United Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: m6A RNA immunoprecipitation was performed using the Magna RIP Kit (Santa Cruz) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The Chronobiology Kit software program (Stanford Software Systems, Santa Cruz, CA, USA) was used to collect ...
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bioRxiv - Cancer Biology 2021Quote: UM-UC-1 cells (2×105) cells were transfected with 2.5 mg of HNF-3alpha CRISPR/Cas9 KO plasmid (Santa Cruz; sc-400743) using Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies were diluted in 2% nonfat dry milk in T-PBS as follows: 1:500 for anti-Cdc42 (Santa Cruz sc-8401); 1:750 for anti-Cdc42 (Santa Cruz sc-87) ...
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bioRxiv - Cell Biology 2020Quote: ... and for Sirt1KO with 2 Double Nickase plasmids each containing a different sgRNA to target sequences 148 CGGACGAGCCGCTCCGCAAG and 110 CCATGGCGGCCGCCGCGGAA (Santa Cruz Biotechnology). For control cells ...
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bioRxiv - Physiology 2020Quote: ... the membranes were washed three times in PBST and incubated 2 h with horseradish peroxidase (HRP) secondary antibodies (1:5000, Santa Cruz Biotechnology). This procedure was followed by three membrane washes with PBST ...
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bioRxiv - Genetics 2019Quote: ... Proteins were separated by SDS-PAGE and detected via Western blot following blocking (in TBS-0.1% Tween-20 with 5% non-fat milk powder) with the following antibody dilutions in the same blocking solution: α-GFP-HRP (B-2, sc-9996, Santa Cruz), 1:5000 ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... was used to collect leaf discs from the inoculation sites three weeks after PVX inoculation for immunoblot with anti-GFP (B-2, sc9996 HRP, Santa Cruz Biotechnology). GFP accumulation was visualised with Pierce™ ECL Western (32106 ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was incubated for 2 h at room temperature in the anti-Myc primary antibody A-14 (1:2000 dilution, lot no. F0810; Santa Cruz Biotechnology), rinsed three times with 1X TBST (0.01 M Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were fixed with 4% paraformaldehyde for 10 mins at 4°C followed by 30mins blocking with 2% BSA and stained with anti-GLP-1 antibody (1:200) (Gt pAb to GLP-1, SC-26637, Santa Cruz Biotechnology) overnight in a humid chamber at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was incubated for 1h at room temperature in blocking buffer (5% nonfat milk in PBS pH 7.4 containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 h at room temperature with antibodies raised against insulin (Santa Cruz Biotechnology Inc, Santa Cruz, CA), the porosome associated protein ATP2C1 and actin (Santa Cruz Biotechnology Inc ...
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bioRxiv - Plant Biology 2022Quote: ... pH 2.2) for two times and applied for the immunodetection of phosphorylation levels by using an anti-pSer primary antibody (Santa Cruz, USA) and antimouse secondary antibody (Santa Cruz ...
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bioRxiv - Immunology 2022Quote: ... 80% confluent HEK293-T cells were transfected with 2 μg of TCR plasmid and 1 μg pCL-Eco helper plasmid with 6 μg (3× DNA mass) PEI (Santa Cruz Biotech) to create retrovirus ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by secondary antibodies tagged with Alexa Fluor® 488 or Alexa Fluor® 568 at room temperature for 30 minutes and 4,6-diamidino-2-phenylindole (Santa Cruz). All samples were assessed under a fluorescence microscope (Leica Microsystem ...
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bioRxiv - Biophysics 2022Quote: ... the immunoblots were blocked with 2% BSA and incubated with primary antibodies: Anti-Lamin A/C antibody (E-1) (sc-376248, Santa Cruz Biotechnology) and Anti-Actin antibody ...
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bioRxiv - Genetics 2022Quote: ... anti-Polycystin-2 (PC2-CT 27, PKD-RCC (https://www.pkd-rrc.org/) and anti-Polycystin-1 (7E12, Santa Cruz cat. no. sc-130554) and secondary labeled antibodies (Licor ...
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bioRxiv - Immunology 2022Quote: ... Blotted proteins were detected with a rabbit polyclonal antibody that recognizes SARS-CoV-2 spike RBD (40592-T62, SinoBiological) and a donkey anti-rabbit IgG-HRP antibody (sc-2077, Santa Cruz Biotechnology).
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bioRxiv - Neuroscience 2022Quote: ... or 10ng/side of PI3K inhibitor 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002; Santa Cruz Biotechnology, Dallas, TX); or vehicle (50% dimethyl sulfoxide [DMSO] in 0.9% NaCl solution ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: anti–pERK1/2 (E-4; sc-7383) and anti– β-actin (C4; sc-47778) from Santa Cruz Biotechnology ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were transduced at varying dilutions of the lentiviral stock (ranging from 1:2 to 1:100) in DMEM + 10% FBS with 8 μg/mL polybrene (Santa Cruz Biotechnologies). Media was refreshed on P19 cells 24 h after transduction ...
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bioRxiv - Molecular Biology 2021Quote: K562 cells at 90% confluency were treated for 2 hours with one of the following reagents (1) pladienolide B (Santa Cruz Biotechnology) in DMSO to a final concentration of 10 μM or (2 ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were then incubated with blocking solution (1% BSA and 10% Donkey serum in freshly prepared 1X PBS) for 2 hrs at RT and then subsequently incubated with primary antibodies: MAP1B at 1:100 (N-19, Santa Cruz Biotechnology), Kif3a at 1:300 (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... seeded at low density and mock-treated or exposed to an increasing dose of UV light (0.5, 1, 2 J/m2 of UV-C 266 nm) or an increasing dose of Illudin S (Santa cruz; sc-391575) for 72 h (15 ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... and the cells were transduced with lentivirus (250 μl/cm2) in Macrophage Maturation Medium containing 2 μg/ml Polybrene (Santa Cruz, sc134220) by spinfection (centrifugation at 1000 x g for 1 h at 32°C) ...
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bioRxiv - Cell Biology 2020Quote: ... containing 5 % nonfat dry milk for 2 h at room temperature and then incubated with anti-NaK-ATPase (1:2500; Santa Cruz Biotechnology), anti-LAMP-1 (1:2500 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 20 ng/ml IL-6 and 4 hours post-treatment lysed using CHAPS buffer and incubated overnight with 2 μg of an anti-ETV7 antibody (TEL2, Santa Cruz Biotechnologies) or normal mouse IgG (Santa Cruz Biotechnologies ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x ...
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bioRxiv - Neuroscience 2022Quote: The sections were then washed with PBS and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Santa Cruz Biotechnology) to visualize nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was incubated in blocking buffer (Amersham Biosciences) for 60 minutes at room temperature and in primary antibody (BRF1/2: CST#2119, GR: CST#3660, GAPDH: Santa Cruz sc32233) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells lysates in Tris pH 7.5 50mM and 2% SDS were applied to SDS-PAGE followed by Western blotting using anti-SLC3A2 (Santa Cruz, 1:5000) and anti-ϒ-tubulin antibodies (1:10,000) ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated overnight at 4 °C with primary antibodies in 0.5% BSA or 2% non-fat milk in T-TBS: anti-AR (1:1000 dilution; Santa Cruz Biotechnology #7305), anti-RUNX1 (1:1000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was then probed with a 1:5,000 dilution of rabbit anti-goat-HRP 2° antibody (Santa Cruz Biotechnology sc-2768), washed ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cell pellets were then incubated with room temperature PBS containing 2 mM DSG (disuccinimidyl glutarate, sc-285455, Santa Cruz Biotechnology) for 25 min with gentle agitation ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was separated by centrifugation at 12.000 g at 4 °C for 10 min and incubated with STAT2 antibody (2 µg) (Santa Cruz Biotechnology, 514193) for 3 h at 4°C with gentle shaking ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology). Transduced cell lines were selected in puromycin and/or blasticidin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were stained for the following antibodies (diluted in 0.2% (v/v) Triton X-100 in Animal Free Blocker and Diluent) overnight at 4°C: CC10 (1:50, Santa Cruz-sc365992), α-smooth muscle actin-Cy3 conjugated (1:50 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778, Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... and lamin A/C N-terminus residues aa 2-29 (LA/C-N, [E-1], sc-376248, Santa Cruz Biotechnology, DA, USA), a rabbit monoclonal Ab (rMAb ...