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Citations for Santa Cruz :
4751 - 4800 of 5084 citations for Mouse Serine threonine protein kinase pim 2 PIM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1 mg of protein was used for Co-IP using anti-HA magnetic beads (Thermo, 88837) or Protein G magnetic beads (Thermo, 10003D) complexed with anti-Heph antibody (Santa Cruz, SC-365365) according to manufacturer’s instructions22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 1-2 μg of anti-eIF4E (sc-271480) antibody for 16 h at 4°C before protein G-PLUS-agarose slurry (Santa Cruz sc-2002) was added for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Nucleus proteins of the ANKRD49-OE or LV5 H1299 cells (1×106) were extracted and 25 µg of them were precleared using Protein A/G Agarose (Santa Cruz, California, USA). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates (500 µg) were pre-cleared with 30 µl of protein A/G plus-agarose beads (Santa Cruz Biotechnology, catalog #: SC-2003) and 1.0 µg of normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... The epitope-tagged Pum and α-tubulin proteins were detected by successive incubation with anti-Myc 9E10 monoclonal primary (SC-40, Santa Cruz Biotechnology) and anti-α-tubulin B-5-1-2 monoclonal primary (T5168 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg of protein were first separated by SDS-PAGE and then blotted with the following antibodies: MSH3 (1:500, Santa Cruz, sc-271080), huntingtin (1:2,000 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections underwent permeabilization with triton-x and were incubated overnight at 4 degrees with 1:500 of goat raised glial fibrillary acidic protein primary antibody (GFAP; SC-6170; Santa Cruz Biotechnologies, Santa Cruz, CA). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: LDLR cell surface protein was quantified by fixing cells in 4% paraformaldehyde and incubated with anti-LDLR at 1:100 (Santa Cruz Biotechnology, sc18823) for 45 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... then soaking the gel for 2 hr with shaking in 1X TBE supplemented with 4.5 mg/mL chloroquine phosphate (Santa Cruz Biotech). The gel was then turned 90° and electrophoresed in the orthogonal direction at 130 V for 60 min in 1X TBE supplemented with 4.5 mg/mL chloroquine phosphate ...
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bioRxiv - Immunology 2019Quote: ... and Homology Directed Repair plasmids containing puromycin resistance gene (MAVS: sc-400769-HDR-2, RIG-I: sc-400812-HDR, both Santa Cruz). The knockout plasmids are a mixture of three plasmids ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were plated in 48-well plates (2×105 cells/well in 600μL) and mixed with polybrene (Santa Cruz Biotechnology, 134220) to a final concentration of 4μg/mL and 100-200μL undiluted lentiviral supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked for 1 h in 10% BSA and 2% normal goat serum (NGS) and incubated overnight at 4 °C with primary antibody (ERα, 1:250, sc-8002, Santa Cruz). Following 3 × 10 min washing in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... GβL (sc-425272) and SUMO1/2/3 deletion were carried out in striatal cells using CRISPR/Cas-9 tools obtained from Santa Cruz, as described before [46 ...
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bioRxiv - Cancer Biology 2022Quote: ... Pfeiffer and Karpas-422 were transduced by spinfection at 1,070 x g (5 acceleration, 2 brake) for 90 min at 37 °C with 8 µg/ml polybrene (Santa Cruz Biotechnology). After 48 h post-transduction ...
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bioRxiv - Biophysics 2019Quote: ... MA, USA), phospho Histone H2A.X (D17A3) (Cell Signalling Technology, Danvers, MA, USA) and CD86 B7-2 (GL-1) (Santa Cruz Technologies) combined with Alexa 647 and Alexa 488 conjugated secondary antibodies (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were pre-incubated for 1-2 h in N2 medium without antibiotics and 1 μg/μl of polybrene (Santa Cruz) to increase transduction efficiency ...
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bioRxiv - Pathology 2020Quote: ... Transfected cells were selected by selection media containing 2 μg/ml puromycin dihydrochloride (cat. no. sc-108071; Santa Cruz Biotechnology, Inc.). After confirming the decrease in LOXL2 expression by reverse transcription-quantitative polymerase chain reaction (RT-qPCR ...
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bioRxiv - Pathology 2020Quote: ... HK-2 cells were treated with media containing 5 μg/ml of polybrene (cat. no. sc-134220; Santa Cruz Biotechnology, Inc.), and then LOXL2 shRNA lentiviral particles and control shRNA lentiviral particles of 1 and 2 multiplicity of infection (MOI ...
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bioRxiv - Genetics 2019Quote: ... then incubated in primary antibody in 1% (w/v) skimmed milk in PBS at the following concentrations for 2-20 h at 4°C (anti-Srs2, Santa Cruz sc-1191 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the mem branes were incubated for 2 h at 37 °C with peroxidase conjugated secondary antibodies against r abbit IgG (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2020Quote: Human T cells (2-5 x 106) were transfected with 3 μg of TERT KO CRISPR/Cas9 plasmid (h) (sc-400316, Santa Cruz) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The pre-cleared chromatin was incubated with 2μg anti-Survivin (Tang, Ling, et al (2012) BBRC 421(2) 249-254) (10811, Santa Cruz Biotechnology), anti-H3K27ac (C15410196 ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Cbl-b (G-1, sc-8006), anti-ERK2 (D-2, sc-1647) and anti-HSC70 (sc-7298) antibodies were purchased from Santa Cruz Biotechnology (Dallas ...
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bioRxiv - Biophysics 2022Quote: Transcription induction of the transfected construct was done 24 h after transfection using 2 μg/mL doxycycline (Santa Cruz # sc-204734B) in Tet-free medium ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse monoclonal anti-HA (1:2000; clone F-7) and anti-PCNA (1:2000; clone F-2) antibodies were from Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... SMAD and Wnt inhibition were discontinued after D3 and the neurons were matured with daily medium changes involving 10µM N-[2S-(3,5-difluorophenyl)acetyl]-L-alanyl-2-phenyl-1,1-dimethylethyl ester-glycine (DAPT; Santa Cruz Biotechnology, SC-201315A), neurotrophic factors (brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were prepared using SDS sample buffer followed by Western Blotting and antibody probing procedures according to the guidelines of the company for the respective antibodies (anti-PPARα: H-2, sc-398394, 1:1000, Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2024Quote: ... All samples and standards had a heavy isotope-labeled nucleoside mix added prior to mass spectral analysis (2′-deoxycytidine-13C1, 15N2 (#SC-214045, Santa Cruz), 5-(methyl-2H3)-2′-deoxycytidine (#SC-217100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1 ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples and standards had a heavy isotope-labeled nucleoside mix added prior to mass spectral analysis (2′-deoxycytidine-13C1, 15N2 (Cat# SC-214045, Santa Cruz), 5-(methyl-2H3)-2′-deoxycytidine (Cat# SC-217100 ...
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bioRxiv - Bioengineering 2023Quote: ... iPSC-CMs were resuspended in replating media (RPMI plus B27 supplement with 2% FBS and 5µM Y-27632 (Santa Cruz Biotechnology)) and 125,000 cells were seeded per fibroTUG substrate through the top of the cell seeding mask in approximately 200 µL of media ...
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bioRxiv - Cell Biology 2020Quote: ... by washing 3 times with phosphate-buffer saline (PBS) followed by incubation with either 10 µg of mouse monoclonal anti-Ndc80/Hec1 antibody (Santa Cruz, sc-515550) or 10 µg of mouse IgG (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... slides were briefly rinsed with PBS and stained overnight at 4°C with antibodies (mouse anti-EPS8, BD Transduction Laboratories Cat#610144, 1:200; rabbit anti-Villin 1:50, Santa Cruz Cat# SC28283) in 1% NGS in PBS ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to an Immobilon-P membrane (MilliporeSigma) and probed with either mouse-anti MAL 1:1000 (clone E-1, Santa Cruz Biotechnology, Inc.) or rabbit anti-ICln 1:1000 (PA5-13450 ...
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bioRxiv - Biochemistry 2022Quote: ... CST, #12697, D8O3J), anti-β-arrestin2 antibody (rabbit monoclonal; CST, #3857, C16D9) and anti-α-tubulin antibody (mouse monoclonal, clone DM1A; Santa Cruz Biotechnologies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C with different primary antibodies: for HEK cells a mouse monoclonal αTRPA1 antibody (1:1000; Santa Cruz Biotech #376495) was used ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: mouse monoclonal antibody against Proliferation Cell Nuclear Antigen (PCNA, sc56 Santa Cruz, CA, USA, 1:50 dilution) and rabbit polyclonal against myeloperoxidase (MPX ...
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bioRxiv - Genetics 2019Quote: ... Membranes were blocked with 5% milk powder in TBS-T and incubated overnight at 4°C with primary antibodies: anti-GFP mouse antibody (Santa Cruz sc-9996) and anti-PGKD1 mouse antibody (Novex 459250 ...
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bioRxiv - Bioengineering 2019Quote: ... Sections were stained overnight at 4°C using unconjugated primary antibodies at 1:100 dilution for endomucin (rat anti-mouse sc-65495, Santa Cruz Biotechnology, Santa Cruz, CA) and at 1:200 dilution for collagen type I (rabbit anti-mouse ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse α-Myc (9E10, 1:500), rabbit α-SKP1 (H-163, 1:1000) and mouse α-ubiquitin (P4D1, 1:1000) were obtained from Santa Cruz. Mouse α-Nucleophosmin (NPM ...
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bioRxiv - Physiology 2019Quote: ... blocked in 5% BSA for 1 hour at room temperature and incubated overnight at 4°C with rabbit polyclonal anti-ABHD2 (1:300) and mouse monoclonal anti-α-actin (1:500, sc-32251, Santa Cruz Biotechnology). The antibody-antigen complexes were visualized by incubation for 1 hour at room temperature with 1:2000 Alexa Fluor 488-conjugated goat anti-rabbit (Molecular Probes A11008/Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2021Quote: ... the membrane was washed three times with Tris buffer and incubated for one hour with anti-mouse antibodies conjugated with horseradish peroxidase (Santa Cruz Biotechnology, USA). After washing the membrane ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were reprobed for Gβ by stripping and then incubating overnight with 1:200 diluted mouse monoclonal anti-Gβ antibody (Santa Cruz #sc-166123) followed by a secondary incubation with 1:1000 diluted m-IgGk BP-HRP (Santa Cruz #sc-516102) ...
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bioRxiv - Cancer Biology 2020Quote: The pH of 500 μl mouse IgG1 HPV 16 E6/18 E6-HRP (CP15) monoclonal antibodies (Santa Cruz Biotechnologies, USA Cat# sc-460) was determined using a pH Meter ...
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bioRxiv - Cancer Biology 2020Quote: ... Gal-3 and Ki67 expression was detected by IHC using mouse polyclonal anti-Gal-3 (Santa Cruz, CA, USA, SC-14364, 1:500) and rabbit polyclonal anti-Ki67 (abcam ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were incubated with goat anti-mouse HRP conjugated secondary antibodies (Novex, A16072) and visualized using luminol reagent (Santa Cruz Biotechnology, sc-2048). Membranes were scanned using the GE LAS 4000 imager (GE Healthscience ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated overnight at 4°C in a humid chamber with 1:200 mouse monoclonal anti-NF-κB p65 antibody (Santa Cruz, CA, USA) in 0.1% Tx-100 ...
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bioRxiv - Microbiology 2021Quote: Triple immunostaining for KS tissues or 3D spheroids implant sections was performed using a mouse anti-PDGFRA (Immunoway, 4G11, YM3688) or anti-Nestin (Santa Cruz, sc-23927) antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then incubated overnight with primary antibodies in 0.3% Triton-X and 5% goat serum in PBS at the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), rabbit anti-FOXG1 (ab18259 Abcam ...