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Citations for Santa Cruz :
4601 - 4650 of 5084 citations for Mouse Serine threonine protein kinase pim 2 PIM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005 ...
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bioRxiv - Neuroscience 2022Quote: ... cell culture supernatants of cultured cerebellar neurons were incubated overnight with Reelin antibody G10 and Protein A/G beads (Santa Cruz Biotechnologies) or brain homogenates were resuspended in immunoprecipitation buffer (25mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates (500 μg) were pre-cleared with 30 μl of protein A/G plus-agarose beads (Santa Cruz, catalog #: sc-2003) and 1.0 μg of normal mouse IgG antibody (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used for Western blot of β-actin and AK2 proteins were: anti-actin (Santa Cruz Biotechnology sc-47778, 1:1000), anti-AK2 (ProteinTech 11014-1-AP ...
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bioRxiv - Immunology 2019Quote: ... immunoprecipitated with 2 µg/sample of indicated antibodies overnight at 4 °C before addition of washed protein A/G PLUS-agarose beads (Santa Cruz Biotechnology) for 1 hr with rotation at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... the protein-retained beads were boiled in Laemmli buffer and immunoblotted using anti-GST and anti-MBP antibodies (Santa Cruz Biotechnology, USA).
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 10 μl of a polyclonal ROCK1 antibody (sc-6055, Santa Cruz Biotechnology) coupled to protein G-Sepharose beads (GE healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... Immunoprecipitated proteins were separated on SDS-PAGE acrylamide gels and western blots were performed using anti-GFP (Santa Cruz Biotechnology sc-9996), anti-SYT1 [7] ...
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bioRxiv - Plant Biology 2019Quote: ... transferred onto PVDF membrane and proteins were detected using an anti-c-myc monoclonal antibody (sc-40, Santa Cruz Biotech, Texas, USA) and anti-mouse HRP conjugate (W4021 ...
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bioRxiv - Cancer Biology 2021Quote: ... to detect DARPP-32 (Cat no.: sc-135877; Dilution 1:200) and α-Tubulin (Cat no.: sc-5286; Dilution 1:1000) protein were obtained from Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... protein lysates (500 μL of NP-40 buffer containing 200 μg of protein) were incubated with 10 μg of primary antibody Neu (F-11) agarose conjugate (sc-7301; Santa Cruz Biotechnology) overnight at 4°C with gentle rotation ...
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bioRxiv - Bioengineering 2020Quote: Tissue sections were incubated overnight with polyclonal rabbit antibodies raised against c-fos protein (1:1000; Santa Cruz Biotechnology Inc.; sc-253), GFAP (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... CBLB and GAPDH in the protein blots were probed with anti-CBLB- and anti-GAPDH-antibodies (SC-8006 and SC-166545, respectively; Santa Cruz Biotechnology). Flow Cytometry analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants were pre-cleared with control IgG for 1 hr at 4 °C and Protein A/G Plus-Agarose beads (Santa Cruz Biotechnology) for 30 min at 4 °C before incubation with primary antibody overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cbl was immunoprecipitated from 20-50 mg of total protein from each treatment using anti-Cbl AB coupled to agarose beads (sc-170 AC, Santa Cruz Biotechnology). The immunoprecipitated proteins were eluted from Cbl AB-agarose beads with Pierce IgG Elution Buffer (21004 ...
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bioRxiv - Immunology 2022Quote: ... WCL were incubated with GLUT3 antibody overnight and then with Protein A/G PLUS-Agarose bead slurry (Santa Cruz Biotechnology, sc-2003) for 3h ...
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bioRxiv - Neuroscience 2023Quote: ... Immunodetection of ubiquitinated proteins was accomplished by incubating the membrane overnight with 1:1000 diluted monoclonal ubiquitin antibody P4D1 (sc-8017; Santa Cruz Biotechnology) and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30 °C for 30 minutes or 1 hour before separation by SDS–PAGE followed by immunoblot analysis for MBP-substrate proteins (Anti-MBP-probe Antibody (R3.2) (Santa Cruz sc-53366)).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aliquots of lysates containing 100 μg of protein were used for each immunoprecipitation reaction with indicated antibodies followed by adsorption to protein A/G PLUS-agarose beads (Cat#: sc-2003, Santa Cruz Biotechnology). DNA-protein cross-link was reversed by heating the samples to 65 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... Precleared extracts were incubated with 40 μL of 50% protein A/G agarose and 5 of normal rabbit IgG (Santa Cruz Biotech), H3K27ac (Active Motif ...
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bioRxiv - Microbiology 2024Quote: ... the formation of a fusion protein with a proper size was validated by Western blotting using anti-myc antibody 9E10 (Santa Cruz Biotechnology), and anti-Xpress tag antibody R91025 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Clarified lysates were incubated with the appropriate antibodies for 2h at 4°C and subsequently with protein A/G agarose beads (Santa Cruz Biotechnology) for 1h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mg of total protein cell lysate was incubated with 4 µg of rabbit anti-human-ERG Ab (Santa Cruz: #sc-28680) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were incubated overnight with RIP immunoprecipitation buffer containing magnetic Protein G beads conjugated with human SFPQ/PSF antibody (sc-271796, Santa Cruz, Cliniscience) or negative control mouse IgG (5415S ...
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bioRxiv - Immunology 2023Quote: ... 1 mg of hTCEpi cell lysate in cell lysis buffer was precleared with 25 μl of protein A/G plus agarose (SC-2003; Santa Cruz Biotechnology) for 2 hours at 4°C with rotation before the agarose was removed by centrifugation at 2,000 rcf for 10 min ...
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bioRxiv - Plant Biology 2024Quote: ... This diluted chromatin was then incubated overnight at 4°C with Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology; sc-2003) and antibodies ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining three aliquots were immunoprecipitated overnight at 4 °C with the indicated antibody mixed with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology). Then ...
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bioRxiv - Plant Biology 2020Quote: ... AmericanBio cat# AB01185) with or without appropriate antibiotics (15 μg/mL ammonium glufosinate (Santa Cruz Biotechnology, cat# 77182-82-2) for vectors pB7-HFN and pB7-HFC ...
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bioRxiv - Cell Biology 2019Quote: ... was diluted at 1:10000 and anti-SYD-2 antibody (cL-19, SC-15655, goat polyclonal, Santa Cruz Biotechnology, USA) at 1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... incubated with the appropriate primary antibody for 2 hours at room temperature: rb-NGF (1:500; Santa Cruz #sc-548), rb-BDNF (1:500 ...
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bioRxiv - Pathology 2019Quote: ... sc-9072, Lot # G0715; 1:1,000, anti-MMP-2, sc-53630; 1:1,000, anti-MMP-9, sc-21733 – Santa Cruz Biotechnology) or beta-actin as endogenous control (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... (2) Quantitative western blot analysis of the complex was performed using a directly labeled 488nM anti-GFP antibody (Santa Cruz). The fluorescence intensity at 488 nm was quantified using an Amersham™ Typhoon Scanner and analyzed with ImageJ ...
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bioRxiv - Cancer Biology 2019Quote: ... The blocked membranes were then incubated overnight at 4 °C with primary antibodies against O-GlcNAc (CTD 110.6 or RL-2, Santa Cruz), arginase-1 (clone H52 ...
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bioRxiv - Plant Biology 2020Quote: ... AmericanBio cat# AB01185) with or without appropriate antibiotics (15 μg/mL ammonium glufosinate (Santa Cruz Biotechnology, cat# 77182-82-2)) ...
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bioRxiv - Cell Biology 2021Quote: ... N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester (DAPT) (1 μM, 24 h, Santa Cruz Biotech) and L-685,458 (1 - 10 μM ...
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bioRxiv - Cancer Biology 2020Quote: ... UMUC1 (2×105) cells were transfected with 2.5 mg of HNF-3alpha CRISPR/Cas9 KO plasmid (Santa Cruz, sc-400743) using lipofectamine3000 (Thermo fisher scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with anti-α-tubulin (1:700, B-5-1-2, Santa Cruz, USA, cat no sc-23948), anti-CENP-C (1:1000 ...
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bioRxiv - Bioengineering 2020Quote: ... bovine serum albumin (2 %) 1 hr before being incubated with anti-RGS9 antibody (1:500; Santa Cruz Biotechnology, SantaCruz, CA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed in 2% formaldehyde solution by adding equal amount of 4% formaldehyde (Santa Cruz Biotechnology, cat# sc-281692) directly in the culture media and incubated for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... PLGA-PEG (100 mg) was dissolved in acetonitrile (2 mL) with either edaravone (100 mg; Santa Cruz Biotechnology, Dallas, TX) for Eda-MNPs ...
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bioRxiv - Developmental Biology 2019Quote: TCam-2 cells were transfected with 40 nM siRNA mixture of 3 different KIF18A sequences (Santa Cruz Biotechnology, sc-96629), PUM1 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultivated four days in the presence of 2.5 μM D-threo-l-phenyl-2-palmitoylarmino-3-morpholino-l-propanol (PPMP; Santa Cruz) to inhibit synthesis of glucosylceramide-based GSLs [41].
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies for IP (NRBP1/2 rabbit, 21549-1-AP –ptg) and mock IP (IgG rabbit, Santa Cruz Biotech, sc-2027) were then added into the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... Phage lysates were treated for 2 hr at 37°C either with 0.01 M methyl methanesulfonate (MMS) (Santa Cruz Biotechnology) or equal volume of phage buffer (100 μM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... media was removed and cells incubated with 20 mL of PBS containing 2 mM disuccinimidyl glutarate (DSG) (Santa Cruz Chemical) for 20 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Cells were then incubated for 2 h with primary antibody anti-α-Syn sc-69977 (Santa Cruz, 1:100 dilution) in blocking buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoblots were visualized by enhanced chemiluminescence (ECL kit, Santa Cruz Biotechnology) after washes ...
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bioRxiv - Molecular Biology 2021Quote: ... and visualized with a chemoluminescence kit (Santa Cruz Biotechnology, sc-2048) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...