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Citations for Santa Cruz :
4701 - 4750 of 5084 citations for Mouse Serine threonine protein kinase pim 2 PIM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Antibodies used for candidate western blots are as follows: mouse anti-E-cadherin (BD Transduction Laboratories, 610181 and Santa Cruz, sc-8426), mouse anti-N-cadherin (Santa Cruz ...
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bioRxiv - Synthetic Biology 2024Quote: ... Blots were then incubated with primary antibody in a 1:1000 dilution with 5% BSA in TBST overnight at 4°C: tyrosine phosphorylation using phosphotyrosine mouse monoclonal Ig pY99 (Santa Cruz Biotechnologies); mCherry expression using anti-mCherry rabbit polyclonal Ig (Rockland Inc.) ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... and blocked for 30 minutes in 10% donkey serum followed by incubation with mouse anti-human SOX18 (1:50; Santa Cruz Biotechnology), rabbit anti-human SREBP2 and UEA1 fluorescently labeled with Alexa Fluor 649 (1:50 ...
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bioRxiv - Neuroscience 2024Quote: Brain sections containing hippocampus were blocked in PBS with 20% BSA and 0.1% Triton for two hours at room temperature prior to incubation with the primary antibody mouse anti-Rem2 (Santa Cruz, sc-514999) at a concentration of 1:100 overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Villin was used as cell loading control and detected using 1:1000 dilution of mouse anti-villin (#sc-373997, Santa Cruz Biotechnology) antibody.
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bioRxiv - Cancer Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: mouse anti-PPM1D (F-10, Santa Cruz, 1:1000), mouse anti-GAPDH (MAB374 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membrane was blocked by Odyssey blocking buffer for 1 hour at room temperature followed by immunoblotting with the following antibodies: mouse monoclonal anti-TRMT1 aa 201-229 (G3, sc-373687, Santa Cruz Biotechnologies), rabbit polyclonal anti-TRMT1 aa 609-659 (Bethyl ...
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bioRxiv - Cell Biology 2024Quote: ... were incubated with 3 μg of anti-SUMO2/3 antibody (Catalog no. M114-3, MBL Life Science) or mouse immunoglobulin G (IgG) (Catalog no. sc-2025, Santa Cruz Biotechnology) with gentle mixing at 4°C for 2 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies were diluted in 5% milk and used 1:10,000 for anti-rabbit IgG (Amersham, NA934) and 1:15,000 for anti-mouse IgG (goat; Santa Cruz Biotechnology, sc-2064). Chemiluminescence signal was detected using the ECL Select detection kit (GE Healthcare ...
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bioRxiv - Genetics 2024Quote: ... Alpha-synuclein was detected using an anti-alpha-synuclein mouse monoclonal antibody (1:500 dilution) (Santa Cruz biotechnology, Santa Cruz, CA, USA). Beta-tubulin was detected using an anti-beta-tubulin mouse monoclonal antibody (E7 ...
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bioRxiv - Genetics 2024Quote: ... Alpha-synuclein was detected using an anti-alpha-synuclein mouse monoclonal antibody (1:500 dilution) (Santa Cruz biotechnology, Santa Cruz, CA, USA). Beta-tubulin was detected using an anti-beta-tubulin mouse monoclonal antibody (E7 ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody solution was prepared with 5% (w/v) skimmed milk in TBST with anti-Parp14 mouse monoclonal antibody (1:500, sc377150, Santa Cruz Biotechnology) with enough volume for two membranes (epitope competition vs control) ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated with the following primary antibody diluted in 5% milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4°C: anti-RPL22 (1:2000, mouse, Santa Cruz sc-373993), anti-RPS6 (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed three times for 10 min with TBS/T and incubated for 1 h with goat anti-mouse IgG-HRP (sc-2005 Santa Cruz Biotechnology) or mouse anti-rabbit IgG-HRP (sc-2357 Santa Cruz Biotechnology ...
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bioRxiv - Physiology 2023Quote: ... The membranes were exposed to primary antibody overnight at 4 C (Phospho eNOS S1179 -BD 612392; Total eNOS/NOS3: BD 610296; anti mouse: Jackson Immuno research 715-035-150; Beta Actin: Santa Cruz sc4778), rinsed and incubated with appropriate horseradish peroxidase-labelled secondary antibody for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands probed with diluted primary antibodies (Table S8) were detected using a goat anti-rabbit or mouse IgG-HRP conjugated secondary antibody (Santa Cruz Biotechnology) and visualized using enhanced chemiluminescence (Amersham Biosciences).
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked in TBS-T supplemented with 5% BSA for 1 h at room temperature before incubating with primary antibody at 1:5000 dilution (mouse Topo IIβ Antibody: clone A2, cat. #sc-365071, Santa Cruz Biotechnology) in TBS-T with 3% BSA overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... For immunohistochemistry and IF staining was performed using DAB or DAPI and the following primary antibodies: anti-ALDH3A1 mouse monoclonal antibody (Santa Cruz Biotechnology), anti-BST2 rabbit polyclonal antibody (Proteintech) ...
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bioRxiv - Cell Biology 2022Quote: ... Immunodetection was performed using VE-cadherin mouse monoclonal antibody (mAb) sc-9989 IgG1 (F-8, C-terminal epitope; Santa Cruz Biotechnology, USA), E-cadherin mouse mAb (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were incubated with 2.5 μg/sample of the primary antibody (goat anti-myc (bethyl #A190-104A) or mouse anti-myc (Santa Cruz #SC-40)) and left to tumble for 1 hr at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1% Tween 20) for 1 h at room temperature and stained with mouse monoclonal anti-GFP (1:2000, Santa Cruz #sc-9996) in 3% milk in TBST at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies for assay of infected cells were chicken polyclonal anti-UL34 (1:250) (71) and mouse monoclonal anti-LMNA/C (Santa Cruz Biotechnology), both diluted 1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... Immunofluorescence was performed as described previously (Sauer et al., 2006) using the InSitu PRO VSI robot (Intavis) and the following antibodies: mouse αHA (Santa Cruz Biotech) 1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... method and Western blotting was performed with a 1:500 dilution of GAL4 (DBD) RK5C1:sc 510-HRP mouse monoclonal antibody (sc-510HRP, Santa Cruz Biotechnology).
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used at a 1:10,000 dilution: mouse anti-rabbit IgG-HRP (Santa Cruz Biotechnology sc-2357, RRID:AB_628497) and mouse IgGκ BP-HRP (Santa Cruz Biotechnology sc-516102 ...
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bioRxiv - Bioengineering 2023Quote: ... They were then incubated with primary mouse GFAP antibody conjugated to Alexa Fluor 647 (1:500, sc-33673 AF647, Santa Cruz Biotechnology) in a blocking buffer for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and rabbit anti-HA (CST, 1:200), rabbit anti-Flag (CST, 1:200) and mouse anti- Su(H) (Santa Cruz, 1:100), mouse anti-NICD (DSHB ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated for 1 hour at room temperature with anti-rabbit secondary antibody (Cell Signalling; 7074) or anti-mouse secondary antibody (Santa Cruz; 516102). Antibodies used were diluted in 5% skim milk in TBST buffer at following dilutions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were fixed using 4% PFA and IF staining was performed using the following primary antibodies: mouse mAb α-LT Cm2B4 (1:1000; Santa Cruz), rabbit mAbs α-VP1 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: Myc- and GFP-tagged proteins were detected by rabbit polyclonal IgG anti-GFP (FL) (1:200; sc-8334 Santa Cruz Biotechnology, Dallas, US) and mouse monoclonal IgG anti-c-Myc (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... A fraction of the mixture of protein-DNA complex was used as “input DNA.” Soluble chromatin was immunoprecipitated with anti-VDRE antibody (D-6, Santa Cruz Biotechnology Inc) and normal mouse immunoglobulin G (IgG ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 20 h at 4 °C in the blocking buffer with goat IgG antibodies specific for the human AIF protein (Santa Cruz Biotechnology, USA) diluted 1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened for gene deletion by PCR as well as LARP1 mRNA and protein depletion determined by qPCR and western blot analysis with a monoclonal LARP1 antibody (Santa Cruz sc-515873). Lesions at the locus of cutting were identified by PCR using genomic DNA to amplify the locus followed by Sanger sequencing and Synthego ICE analysis.
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bioRxiv - Developmental Biology 2022Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels with the following antibodies anti-NFATc1 (1:1000, clone 7A6, Santa Cruz) and anti-GAPDH (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... A precleaning step of the samples was performed by adding 50 μl of Protein G-Plus agarose beads suspension (Santa Cruz Biotechnology, INC) to 1 ml of each sample (Chimera ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in RIPA buffer and 100 μg of total protein were immunoprecipitated with an antibody recognizing total Stat92E (Santa Cruz, sc-15708) at 4 °C overnight ...
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bioRxiv - Biochemistry 2019Quote: ... When blotting for total proteins (Cell Signaling Technology; EGFR #4267, p44/42 MAPK #4695, β-Actin #3700 and Santa Cruz Biotechnology; PTPRJ #376794), the membranes were blocked with 5% dry milk in TBS-T for 1 h at RT ...
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bioRxiv - Immunology 2019Quote: ... The immunoreactive proteins were visualized with horseradish peroxidase (HRP)-conjugated secondary antibody (polyclonal goat anti-rabbit IgG, Santa Cruz Biotechnology, cat.no. sc-2004) and SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... at 4 °C followed by 3–18 h rotated incubation with protein A/G agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... at 4 °C followed by 3–18 h rotated incubation with protein A/G agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: Coimmunoprecipitation was performed as previously described (Waxman, Baconguis, Lynch, & Robinson, 2007) with the following substitutions: Protein A/G agarose (Santa Cruz Biotechnology, Santa Cruz, CA) was used to bind 3ug of antibody ...
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bioRxiv - Genetics 2020Quote: ... Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030, Santa Cruz Biotechnology) at 1:2,000 – 1:4,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blocked using either 5% BSA (Bovine Serum Albumin) in Tris buffer Saline containing 0.1% Tween (TBST) for phosphorylated proteins or 5% Blotto (Santa Cruz Biotechnology, sc-2325) for 60 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and expression was detected using the following antibodies: ATF3 (sc-81189, Santa Cruz Biotechnology, Dallas, TX, USA), cleaved caspase-3 (#9664 ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was transferred to a new micro-tube and precleared using 10 μl of protein A/G bead (Santa Cruz Biotechnology, sc-2003). It was then incubated with 0.5 μg of anti-Myc antibody for 1 hr at RT and incubated 20 μl of protein A bead for O/N at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
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bioRxiv - Microbiology 2023Quote: ... To determine interaction between BicDR1 and the viral capsid protein p24 cells were permeabilized and blocked using 3% donkey serum followed by incubation with primary antibodies targeting p24 (Santa Cruz sc-69728) and BicDR1 (Atlas Antibodies HPA041309) ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...