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Citations for Santa Cruz :
5001 - 5050 of 5084 citations for Mouse Serine threonine protein kinase pim 2 PIM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... the protocol was modified by adding the primary antibody recognizing the target (Krt8, rabbit, Abcam, EP1628Y, 1/100; Aqp1, mouse, Santa Cruz, sc-25287, 1/50) to the anti-DIG-AP antibody mixture and incubation O/N at 4 °C (Lopez ...
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bioRxiv - Neuroscience 2021Quote: ... Western blot analysis was performed with the following primary antibodies: mouse monoclonal anti-D3R (1:100; sc-136170, Santa Cruz Biotechnology Inc., Dallas, Texas, USA); rat monoclonal anti dopamine transporter (DAT ...
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bioRxiv - Neuroscience 2021Quote: ... The reads were mapped with TopHat v.2 software (CCB, Johns Hopkins University, Baltimore, MD, USA) to the UCSC mm10 mouse reference (Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA). Biological quality control and summarization were performed with RSeQC-2.3.3 and PicardTools1.92 ...
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bioRxiv - Immunology 2020Quote: ... Membranes were washed and incubated for 1 hr at room temperature with the appropriate HRP-labeled pre-absorbed goat anti-rabbit or anti-mouse secondary antibodies (Santa Cruz Biotechnology, Inc.; Dallas, TX). The membranes were washed ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were collected and precleared by incubating with 1 μg of mouse anti-IgG (Cat. No. sc-2025; Santa Cruz Biotechnology [SCB], Dallas, TX) and 20 μL of protein A/G-PLUS agarose beads (Cat ...
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bioRxiv - Developmental Biology 2024Quote: ... MPI-CBG antibody facility and a kind gift from Prayag Murawala) and anti-PCNA (mouse) conjugated with Alexa 647 (Santa Cruz PC10, sc-56 AF647) was done in a blocking solution 1:100 overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: Nitrocellulose was incubated in blocking buffer (PBS containing 3% powdered skimmed milk) for 30 minutes at rt and then incubated with primary mouse anti-DOK7 antibody (Santa Cruz cat no sc-390856) diluted 1:250 in blocking buffer for 1 hour at rt with gentle agitation ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... After washing in TBST the membranes were incubated in a secondary antibody with HRP (anti-rabbit, Vector pI-1000, 1:5000; anti-mouse, Santa Cruz, sc-2005, 1:5000) and washed again ...
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bioRxiv - Bioengineering 2022Quote: ... the samples were incubated with mouse primary antibody HSP70 (1:50, cat. no SPA-810; Enzo Life Science, Santa Cruz Biotechnology, Paso Robles, CA, USA) diluted in blocking solution overnight (ON ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used for the HCT116 H2B-GFP ABAT knock down validation were mouse anti-ABAT (1:500; Santa Cruz Technology, Inc., #sc-393769) and mouse anti-Actin (1:10000 ...
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bioRxiv - Physiology 2023Quote: ... Blots were blocked with LI-COR PBS-blocking buffer and probed with the following primary antibodies at 4°C: anti-CD63 (1:1000 dilution; Cat#SC13118, Santa Cruz, mouse monoclonal, RRID: AB_627213) and anti-CD9 (1:1000 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... and an α-GFP antibody (α-GFP, life technologies, cat # A11122, 1:5000; goat anti-mouse IgG-HRP, Santa Cruz, Cat # sc-2005, 1:10,000). Likewise ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted fractions were loaded in the SDS gel and western blot was performed with anti-mouse p63 and p73 primary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA)(1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-p-Histone H3-Ser10 (pH3) monoclonal antibody (CST, #53348; 1:100) and mouse anti-CK15 monoclonal antibody (Santa Cruz, sc-47697; 1:200). The following secondary antibodies were used ...
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bioRxiv - Cell Biology 2024Quote: ... and a 1:100 dilution of anti-stage-specific embryonic antigen-1 (SSEA-1) mouse IgM antibody (sc-2170; Santa Cruz Biotechnology, Dallas, TX, USA) with 1% blocking reagent TNT for 1 hour on ice ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Molecular Biology 2021Quote: 300 million TetR-CBX7 reporter mESCs were infected with a 1:10 dilution of the harvested virus-containing supernatant PlatinumE cell medium for 24 hrs in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). The 300 million cells were divided into 3x 100 million sets (10x 15 cm plates of 10 million cells each ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked and probed in TBST-5% non-fat milk using anti-GFP (HRP-conjugated B-2, sc-9996 HRP, Santa Cruz, 1:5000 dilution) or anti-HA (HRP-conjugated ...
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bioRxiv - Biophysics 2020Quote: ... using EZ-Retriever V.2 (BioGenex, San Ramon, CA, USA) and immunostained with anti-α-SMA monoclonal antibody (Cat No.sc-32251, Santa Cruz Biotechnology, CA, USA) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and β5-integrin cells were incubated for 20 min before chase and during pulse with 10 μM gefitinib (Santa Cruz Biotechnology, 184475-35-2), 10 μM PP2 (Thermo-Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... placed in a blocking solution (0.5% TX-100 in PBS + 3% NDS) and then incubated for 2 days at 4°C in goat anti-calbindin (1:200; Cat#SC7691; Santa Cruz Biotechnology, Dallas, Texas), followed by 2.5 hours incubation at RT of secondary antibody coupled to a green fluorophore (1:200 ...
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bioRxiv - Pathology 2019Quote: ... Cell lysates of equal protein amounts were incubated overnight at 4°C (constant rotation) with 2 μg of an anti-HA antibody or immunoglobulin G (IgG) control (sc2027; Santa Cruz Biotechnology, Dallas, TX). Co-immunoprecipitation of STAT3 with associated proteins was then performed by using Protein A/G Agarose beads included in the Pierce Classic IP Kit (26146 ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hour at room temperature before probing with a 1:200 dilution anti-vinculin 7F9 (Santa Cruz Biotechnology; sc-73614) in 5% BSA/1x TBST for 1 hour at room temperature followed by anti-mouse IgG HRP secondary ...
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bioRxiv - Genetics 2021Quote: ... 300 million #H5 clone mESCs were infected with a 1:10 dilution of virus-containing supernatant for 24 h in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220) (Before infection ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies used for immunofluorescence were Rat Anti-C-peptide (Developmental Studies Hybridoma Bank, ID-4) and Goat Anti-GLP-2 (Santa Cruz Antibodies, C-20).
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bioRxiv - Evolutionary Biology 2023Quote: ... cells were blocked in 3% BSA in PBS with 0,2% Tween 20 and incubated overnight with anti-MYC (A-14, Santa Cruz Biotechnology, 2 ug/ml), anti-CENP-C (PD030 ...
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bioRxiv - Immunology 2023Quote: ... Lungs were dissected at the stated time post tumor initiation and fixed for 24 hours in 2% paraformaldehyde (Santa Cruz Biotechnology Cat# sc-253236) at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed from dishes and assessed by flow cytometry for construct expression and selected in puromycin 2 μg/mL (Santa Cruz Biotech, sc-108071B). Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with PB-vector constructs as above ...
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bioRxiv - Cancer Biology 2023Quote: ... were used to produce HCT116 H2B-GFP ABAT knock down cells at a multiplicity of infection (MOI) of 5 and 2 mg/mL of puromycin (Santa Cruz Biotechnology, #sc-108071) was used to select for knocked down cells ...
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bioRxiv - Cell Biology 2024Quote: Cells in growth medium without penicillin and streptomycin were transfected with the hTRPV4 shRNA plasmid (1 and 2 µg; Santa Cruz sc-61726-SH) and Lipofectamine3000 in opti-MEM ...
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bioRxiv - Microbiology 2019Quote: ... 100 μl of serially-diluted serum samples were incubated for 1 h at room temperature and subsequently detected using HRP-conjugated goat anti-mouse IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA). 3,3’,5,5’-tetramethylbenzidine peroxidase substrate solution (KPL ...
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bioRxiv - Systems Biology 2022Quote: ... the membrane was washed 3 times with PBS-T buffer and then incubated at room temperature for 1 h with secondary antibodies: mouse monoclonal anti-rabbit IgG-HRP (Santa Cruz Biotechnology, cat. no. sc-2357), goat monoclonal anti-mouse IgG HRP (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2020Quote: Sections from formalin fixed paraffin embedded mouse tissue or a human pancreatic cancer tissue microarray (Biomax PA961e) were stained with antibodies against PC (Santa Cruz sc-67021, 1:50 dilution) or ME1 (Proteintech 16619-1-AP ...
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bioRxiv - Microbiology 2021Quote: ... were incubated for 45 min at rt using a rabbit polyclonal antibody (pAb) anti-ACE2 (Abcan ref # ab15348) and a mouse monoclonal antibody (mAb) anti-TMPRSS2 (Santa Cruz Biotechnology, Inc. ref # sc-515727). Followed by 30 min incubation at rt with a goat anti-rabbit IgG (goat anti-rabbit IgG ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies used in the present study are listed as follows: anti-mouse IgG-HRP (MBL, Nagoya, Japan) and goat anti-rabbit IgG-horseradish peroxidase (HRP; Santa Cruz Biotechnology, Santa Cruz, CA, USA). Each experiment using western blotting was replicated three or four times.
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bioRxiv - Microbiology 2022Quote: ... Briefly 3 x105 cells were incubated on ice with mouse anti-human ACE2 monoclonal antibody conjugated to AF-647 (Santa Cruz Biotechnology, USA, cat. SC-390851) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... for 5 minutes and incubated at room temperature for 1 hour in the dark with a fluorescent secondary antibody mixture contaning mouse IgGk BP-CFL594 (Santa Cruz Biotech, sc-516178, 1:100) and anti-rat IgG AF488 (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... skimmed milk in PBS at the following concentrations for 30-120 min at RT (anti-goat, Santa Cruz sc-2020, 1:2500; anti-mouse, Santa Cruz sc-2005, 1:2500). Membranes were washed 3x in PBS ...
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bioRxiv - Genetics 2019Quote: ... incubated for 1 hour at room temperature in the appropriate secondary antibodies diluted 1:10,000 in 5% nonfat dried milk in TBST (goat anti-rabbit IgG-HRP, Santa Cruz Biotechnology, Cat#sc-2004; goat anti-mouse IgG-HRP, Santa Cruz Biotechnology, Cat#sc-2005). Membranes were washed 3 x 10 minutes in TBST ...
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bioRxiv - Neuroscience 2022Quote: ... A subset of sections from brains with CaMKII-SomArchon expression were stained with a mouse anti-CaMKIIα/β/γ/δ antibody (sc-5306, Santa Cruz, 1:50) followed by Alexa Fluor 568 goat anti-mouse secondary antibody (No ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then blocked in 3% goat serum for 1 hour at room temperature and incubated in mouse anti-Sag1 (28) [DG52] (gift from John Boothroyd, 1:5000) and anti-NF-κB (p65) (Santa Cruz Biotechnology, sc-372, 1:1000) antibodies overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were stained with the following primary antibodies diluted in Intercept (TBS) Blocking Buffer (LiCor, 927-60001): mouse anti-BRAF (Santa Cruz Biotech, sc-5284, 1:200), mouse anti-ERK (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... was blocked in 5% milk for one hour at room temperature and incubated with a monoclonal mouse anti-GFP antibody (Santa Cruz Biotechnology sc-9406, 1:2500), a rabbit anti-pRab8A phospho T72 monoclonal antibody (Abcam 230260 ...
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bioRxiv - Microbiology 2024Quote: ... containing 10% milk for one hour and then incubated overnight at 4°C with anti-PARP-1 mouse monoclonal antibody C2-10 (Santa Cruz Biotechnologies sc-53643, 1/500) or a rabbit polyclonal IgG (Santa Cruz Biotechnologies sc-7150 1/1000) ...
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bioRxiv - Neuroscience 2019Quote: EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328, Santa Cruz Biotechnology; ratio: 1:1) for 2 h at room temperature prior to the addition to the cells(26).
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bioRxiv - Cell Biology 2021Quote: ... Then cells were incubated with Lamp1anti-goat primary antibody (1:250, sc-8099, Santa Cruz Bio-technology; Santa Cruz, CA, USA; 2 h, 4 °C) in the blocking buffer for 45 min ...
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bioRxiv - Genomics 2021Quote: ... iPSC-derived MSCs were cultured to at least passage 6 and seeded at 4.2 × 104 cell/cm2 in culture dishes (4-10cm diameter) coated in 5ug/cm2 Type I Collagen (sc-136157, Santa Cruz Biotechnology, Dallas, TX, USA). After culturing cells for 1 day in MSC medium ...
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bioRxiv - Biophysics 2020Quote: ... A mixture of 0.5 µm Alexa Fluor 488 lysine labeled NSARS-CoV-2 and 1:100 Alexa Fluor 594 conjugated G3BP1 antibody (Santa Cruz Biotechnology, sc-365338 AF594) in cell permeabilization buffer was added to the cells and movies were recorded with 512 x 512 pixel resolution at 1000 Hz speed and 1 s per frame for about 2-3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was incubated for 1h at room temperature in blocking buffer (5% nonfat milk in PBS pH 7.4 containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 h at room temperature with antibodies raised against insulin (Santa Cruz Biotechnology Inc, Santa Cruz, CA), the porosome associated protein ATP2C1 and actin (Santa Cruz Biotechnology Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... 1/10000) (Zhu et al. 2010), anti-Red1 (chicken, 1/3000) (Shinohara et al. 2008) and anti-α-tubulin (YL1/2, Santa Cruz Biotechnology, 1/3000). Tubulin signals were visualized using the LI-COR Bioscience system or alkaline phosphatase stain (BCIP-NBT solution kit ...