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Citations for Miltenyi Biotec :
3101 - 3150 of 3163 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: To assess immune cells and endothelia from the lung left lobe of lung was digested using a GentleMACS lung digestion kit (Miltenyi Biotec #130-095-927). In brief ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Conidia suspensions were prepared by rinsing the plates with sterile distilled water and passed through a 20-µm cell strainer (Miltenyi Biotec, Bergisch Gladbach, Germany) to eliminate residual mycelium ...
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bioRxiv - Cancer Biology 2023Quote: ... and later monocytes were enriched from the total bone marrow cells using the Monocyte Isolation Kit (BM) from Miltenyi Biotec (Cat: 130-100-629) through a negative selection process following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... dead cells were removed from the culture via a Miltenyi Biotec Dead Cell Removal Kit and MiniMACs Separator with accompanying columns (Miltenyi Biotec, Bergisch Gladbach, Germany). CD61+ Mks were then enriched from the live cells using CD61 MicroBeads and a MidiMACs Separator with accompanying columns (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... CD61+ Mks were then enriched from the live cells using CD61 MicroBeads and a MidiMACs Separator with accompanying columns (Miltenyi Biotec, Bergisch Gladbach, Germany). The resulting sample of live CD61+ Mks was resuspended in IMDM media supplemented with BIT 9500 ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8 T lymphocytes were purified from total PBMC by Naïve CD8+ T Cell Isolation Kit and MACS® separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: We isolated human umbilical cord blood (hUCB) CD34+ cells from two patients using the Miltenyi CD34 Microbead Kit and MACS separation column (Miltenyi Biotec, Boston, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... immune cells from the colonic lamina propria were extracted using the Lamina Propria Dissociation Kit for mice and gentleMACS dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... cells were rinsed 1X with PBS and 1X with autoMACS running buffer (MACS Separation Buffer (130-091-221, Miltenyi Biotec, San Diego, CA, USA). The cardiac Troponin T (TNNT2 ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
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bioRxiv - Developmental Biology 2023Quote: ... dissociation was performed for 55 mins (mature sample) or 45 mins (blastema samples) and the cells were filtered through a 70 μm MACS SmartStrainer (Miltenyi Biotec 130-098-462). Cells were analysed by FACS (FACSAria III Cell Sorter ...
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bioRxiv - Cancer Biology 2023Quote: Cell sorting was performed on Control and EIF4G2KD HEC-1A cells following magnetic beads separation of CD133+ and CD133-(CD133 Microbead Kit, Miltenyi Biotec, 130-097-049) and LS-columns (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2023Quote: ... with the following modifications: dissociation was performed for 55 mins and the cells were filtered through a 70 μm MACS SmartStrainer (Miltenyi Biotec 130-098-462). Anterior cells (mCherry-positive plus EGFP-negative ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was dissociated into a single cell suspension and the debris were removed using the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677) and gentleMACS program 37C_ABDK_02 according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Harvested brain tissue was converted to single cell suspensions using the papain-based mouse adult brain dissociation kit from Miltenyi Biotec (130-107-677), with appropriate steps for myelin removal and red blood cell lysis according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Freshly enriched EVs from WJ-MSC WT and CD81-Snorkel-tag cell supernatants were immunoprecipitated either with hCD81 exosome isolation kit (Miltenyi biotec; 130-110-914) or µMACS™ HA Isolation Kit (Miltenyi biotec ...
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bioRxiv - Immunology 2024Quote: Peritoneal washes from ID8-bearing mice were collected on days 41-47 after ID8 tumor cell injection as specified and F4/80+ macrophages were sorted with magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) with the typical purity yield >95%.
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched from bulk tumor cell suspensions using CD45 (TIL) mouse Microbeads kit followed by magnetic separation on LS columns (Miltenyi Biotec, Bergisch-Gladbach, Germany). After eluting CD45+ cells from the column ...
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bioRxiv - Immunology 2024Quote: ... The lungs were collected in free RPMI medium and then dissociated for single-cell suspension by the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells counts were determined by flow cytometer Novocyte (Agilent Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were centrifuged and resuspended in a 5% fetal bovine serum in PBS (FBS/PBS) with CD133-PE (Miltenyi Biotec 130-080-801), CD44-FITC (BD Pharmingen 555478 ...
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bioRxiv - Cancer Biology 2021Quote: ... iPS cells were seeded on previously coated Laminin 521 (Biolamina, Sundbyberg, Sweden) plates to support cell viability in StemMACS™ iPS-Brew XF (Miltenyi Biotec, Bergisch Gladbach, Germany) supplemented with 10 µM of Rock inhibitor Y-27632 and 1x CloneR (Stemcell Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... CD146+ pericytes were isolated from digested cell suspensions of Scx+ or ScxGFP mice using anti-CD146 magnetic microbeads (130-092-007, Miltenyi Biotec, San Diego, CA, USA), and placed into 24-well tissue culture dishes (Corning ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... CD45− cells were enriched by autoMACS separation after magnetic labeling of CD45+ cells using anti-CD45-APC followed by anti-APC microbeads (Miltenyi Biotec, Cat. #130-090-855) or anti-CD45 Nanobeads (Biolegend ...
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bioRxiv - Molecular Biology 2022Quote: Healthy and CLL splenic B cells were isolated from one-year-old WT and Eμ–TCL1 mice by positive B-cell selection with CD19 magnetic beads (CD19 MicroBeads, Miltenyi Biotec, cat. 130-121-301). Briefly ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
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bioRxiv - Bioengineering 2022Quote: ... and then the cell pellet was resuspended in 100 µL of MACS buffer and 25 µL of anti-integrin α-7 microbeads (Miltenyi Biotec, #130-104-261) for incubation at 4 °C for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Ly6G+ cells were positively selected to isolate G-MDSCs from Ly6G- M-MDSCs and were passed through LS columns (Miltenyi Biotec, cat. 130-042-401) twice to increase purity ...
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bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were isolated from mouse lung tissue (without the trachea) using the QuadroMACS™ Separator and antibody-bound magnetic beads (Miltenyi Biotec, Leiden, The Netherlands). EpCAM+ cells and fibroblasts were mixed 1:1 (20,000 cells each ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were washed 3x with MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]). After removing supernatant from final wash ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi tissues were dissociated to single-cell suspension by enzymatic degradation using a MACS Technology neural tissue dissociation kit (Miltenyi Biotec, Cat# 130-092-628) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The brain tissues from two animals were pooled in every group and processed for live single-cell suspension preparation using gentleMACS™ Tissue Dissociator (Miltenyi Biotec, Gaithersburg, MD, USA). The cell suspensions were incubated with CD11b microbeads (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2024Quote: ... Neurospheres and attached cells were resuspended in scRNA-seq buffer (HBBS + 0.04% BSA, Fisher Scientific #14-175-095 and Miltenyi Biotec #130-091-376, respectively) and washed 3 times ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cut along the coronal plane with a razor into three pieces and digested into cell suspensions using a Multi Tissue Dissociation kit 1 (Miltenyi Biotec, Germany; #130-110-201) and a gentleMACS™ OctoDissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2022Quote: ... and lungs were processed into single-cell suspensions with a gentle MACS™ Octo Dissociator and Mouse Lung Dissociation Kit (Miltenyi Biotec, #130-095-297) according to manufacturer protocols.
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bioRxiv - Immunology 2023Quote: ... T cells and low-density granulocytes were depleted with anti-CD3 and anti-CD15 microbeads using a QuadroMACS separator (Miltenyi Biotec, Cat. 130-090-976) and LS columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... the pellet was incubated with RBC lysis buffer for 5 min on ice to remove red blood cells (RBC) and then used for Treg isolation with a CD4+CD25+ Treg isolation kit (Miltenyi Biotec, cat # 130-091-041), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in 500 μl of MaCSQuant® Tyto® running buffer and analysed with MACSQuant® Tyto® Cell Sorter (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... target U937 cells (killing-sensitive) and control K562 cells (killing-resistant) were pre-treated with soluble anti-CD3 monoclonal antibody (mAb; OKT3, Miltenyi Biotec, at 200 ng/ml) for 15 min at 37°C prior to co-culture with primary human CD8+ T cells ...
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bioRxiv - Cell Biology 2023Quote: ... the donor liver is chopped into small pieces before undergoing enzymatic digestion and CD133 selection via magnetic activated cell sorting (Miltenyi Biotec, Cat#170-076-719). Primary human HPC are routinely expanded as 3D organoids suspended in Matrigel (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were positively selected using anti-CD4 and CD8 antibodies through the Prodigy TCT program on a CliniMACS Prodigy (Miltenyi Biotec, Cat# 200-075-301). After selection ...
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bioRxiv - Cancer Biology 2023Quote: ... a fixed number of cells were used to start the TCT program on Prodigy using a TS520 tubing set (Miltenyi Biotec, Cat# 170-076-600). Cells were cultured in Optimizer medium (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: MEFs were seeded at 5.104 cells per well in 24-well plates one day before stimulation and treated with 300 IU/mL IFNα (Miltenyi Biotec Cat#130-093-131) and incubated for up to 24 hours.
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... PBMCs were stimulated for 3 hours with 10 μg/mL of Gag AK11 peptide and T cells producing IFN-γ in response were enriched using the IFN-γ Secretion Detection and Enrichment Kit (130-054-201; Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Naïve mouse B-cells were isolated from the spleen of 7–10 weeks old wild type or c-mycf/f mice with the B-cell isolation kit (MACS Miltenyi Biotec Cat. no. 130-090-862). After purification ...