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Citations for Miltenyi Biotec :
2951 - 3000 of 3163 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... B lymphocyte isolation was conducted through negative selection by using the B cell Isolation Kit II (Miltenyi Biotec, Cat#130-091-151) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... stained samples were diluted 10-fold in 1x PBS and cell concentrations as well as green fluorescence intensities were recorded on a MACSQuant Analyzer 10 (Miltenyi Biotec, Germany).
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bioRxiv - Immunology 2022Quote: ... Then 1.5x 105 cells/cm2 in 24-well dishes were seeded and mouse receptor activator of nuclear factor kb ligand (RANKL, Miltenyi Biotec, Germany) was added at the final concentration of 35 ng/ml to initiate osteoclasts (OC ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended and incubated with propidium iodide to exclude dead cells prior to analysis with the Macsquant analyzer (Miltenyi biotec, Bergisch Gladbach, Germany). For kinetics analysis of transferrin recycling ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... hiPSC-aCMs and -vCMs at day 20-30 post-differentiation were dissociated into single cells which were then enriched using a MidiMACS PSC-derived Cardiomyocyte Isolation Kit (Miltenyi Biotec, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were purified by positive selection and monocytes were isolated by negative selection according to the manufacturer’s instructions (Miltenyi Biotec, Woking, UK).
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bioRxiv - Immunology 2019Quote: ... The cytoplasmic domain comprised of a truncated CD271(ΔLNGFR) gene for the isolation of T cells expressing CAR was amplified from pMACS-ΔLNGFR (Miltenyi Biotec, Germany) and overlapped with the P2A oligonucleotide ...
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bioRxiv - Cell Biology 2019Quote: ... The suspension was incubated for 15 min with dead cell removal paramagnetic beads (130-090-101, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and then passed through LS column (130-042-401 ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells from MLNs of Hpb cured VillinCre-A2BARfl/fl and corresponding controls VillinCre infected mice were magnetically sorted from MLN and spleen single cell suspensions using mouse CD4 MicroBeads (Miltenyi Biotec, Inc.) sorted on positive selection columns ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 480 μL staining buffer before adding 120 μL anti-mouse IgG2a/b MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for an incubation period of 15 min ...
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bioRxiv - Immunology 2021Quote: ... Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec, USA). To obtain high purity Treg ...
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bioRxiv - Immunology 2022Quote: γδ T cells were sorted from the spleen of MCMV infected or control TCRα-/- mice using the TCR γδ T cell Isolation kit (Miltenyi Biotec). For γδ TCM and TEM sorting ...
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bioRxiv - Cancer Biology 2022Quote: ... Neutrophils and the other cell types were isolated from the suspension using the MACS Neutrophil isolation kit (Miltenyi Biotec, 130-097-658) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Gently absorbed mononuclear cell layer was resuspended in PBS to centrifuge at 500g for 5min and the resuspended cell pellets were incubated with CD3 Microbeeds (130-050-101, Miltenyi Biotec, Germany) at 4-8°C for 15min ...
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bioRxiv - Immunology 2022Quote: ... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...
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bioRxiv - Immunology 2022Quote: ... for preparation of single cells as per manufacturer’s instruction using a gentleMACS™ Octo Dissociator with heaters (Miltenyi Biotec; 130-096-427). Cells were passed through a 70 μm filter and washed twice using ice-cold PBS followed by RBC lysis using ACK lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Epithelial cells were isolated from colon samples in the pre-digestion buffer using the Lamina Propria Dissociation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... A purified monocyte fraction was isolated from the thawed cells using the negative selection MACS® Pan Monocyte Isolation Kit (Miltenyi Biotec) with LS columns according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: BAL samples from silica beads and silica treated mice were treated for 10 min with red blood cell lysis solution (Miltenyi Biotec, 170080033) and washed with PBS by centrifugation at 300 g for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were spun at 400 g for 3 minutes at 4°C and washed in MACS Buffer (Miltenyi Biotec, 130-091-222) supplemented with 4% FBS (FBS ...
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bioRxiv - Genomics 2024Quote: ... The resulting cells were enriched for microglia with magnetic labeling and positive selection using CD11b MicroBeads (Miltenyi Biotec cat. #130-093-634) and LS columns ...
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bioRxiv - Genomics 2024Quote: ... Myelin were removed from the single-cell suspensions using negative selection with Myelin Removal Beads II (Miltenyi Biotec cat. #130-096-733) and LS columns (Miltenyi Biotec cat ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... Yeast cells were washed 5 times with PBSF (PBS + 0.1% bovine serum albumin (BSA)) and 108 cells were incubated with 400 μL PBSF and 100 μL μMACS™ anti-c-Myc MicroBeads (Miltenyi Biotec) for 45 min on a rotator at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... single-cell suspensions were generated by dissociation of the organoids with a papain-based neural dissociation kit (Miltenyi Biotec, 130-092-628). Briefly ...
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bioRxiv - Physiology 2023Quote: ... The suspension was incubated for 15 min with dead cell removal paramagnetic beads (130-090-101, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and then passed through LS column (130-042-401 ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from RBC-depleted splenocytes using the Miltenyi CD8+ negative selection kit (Miltenyi Biotec, Cat # 130-104-075). Reporter-expressing CD8+ T cells were sorted using a BD Aria III ...
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bioRxiv - Immunology 2023Quote: ... naïve 8.3 CD8 T cells from the LNs of non-diabetic NY8.3 NOD mice were purified using two rounds of the naïve CD8 T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... The anti-IgA-stained bacteria were then incubated in 1 mL of staining buffer containing 50 μL of anti-PE magnetic activated cell sorting (MACS®) beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells from mice immunized with SRBC for 10 days were pre-enriched by B220 MicroBeads (Miltenyi Biotec; 130-049-501) followed by flow sorting ...
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bioRxiv - Cell Biology 2023Quote: ... we purified some of the myoblast cultures using the mouse Satellite Cell Isolation Kit (130-104-268, Miltenyi Biotec, Bergisch-Gladbach, Germany) when necessary due to higher fractions of fibroblasts (e.g. ...
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bioRxiv - Biophysics 2023Quote: ... CD4+ and CD8+ T cells were positively sorted from the PBMCs utilizing the StraightFrom® Buffy Coat CD4/CD8 MicroBead (Miltenyi Biotec) and subsequently separated using the QuadroMACS™ Separator (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2023Quote: ... Trogocytosis was measured as the acquisition of CAF-specific fluorescence in PDAC cells by flow cytometry on MACSQuant® VYB Flow Cytometer (Miltenyi Biotec) or FACScan analyzer (BD Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: ... tumor spheroids cultured in a 96-well low-adhesion plate or within a hole within a microfluidic device were dissociated into single cells using TrypLE and receive FcR Block (Miltenyi Biotec, Germany), were stained with BV421-CD45 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in 400 μl of buffer with 30 μl of CD31 MicroBeads (130-097-418; Miltenyi Biotec, Bergisch Gladbach, Germany), 30 μl of CD45 MicroBeads (130-052-301 ...
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bioRxiv - Genetics 2024Quote: ... The live cell fraction was pelleted and resuspended in 1X PBS with 0.04% bovine serum albumin (BSA, Miltenyi Biotec # 130-091-376) to a final concentration of 1 × 106 cells/mL (1,000 cells per μL).
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bioRxiv - Cancer Biology 2024Quote: The biopsy and surgical samples used to prepare the single cell suspension were immediately put into a tissue preservation solution (Miltenyi Biotec, Germany) and transported to the field laboratory in an ice bath for immediate preparation of the single cell suspension ...
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bioRxiv - Immunology 2024Quote: ... Tregs were purified from the mouse lymphocytes using a CD4+CD25+ regulatory T cell isolation kit (Miltenyi Biotec, cat. # 130-091-041). The purity of the isolated cells was checked by flow cytometry ...
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bioRxiv - Molecular Biology 2024Quote: ... The CD34+ cell fraction was then isolated from the MNCs using the MidiMACS system (CD34+ Microbead Kit; Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: ... both CD45.2 DKO and WT polyclonal donor marrow was depleted of mature T cells by magnetic column-based negative selection (Miltenyi Biotec MACS) using an anti-mouse CD5 biotin-conjugated antibody (Clone 53-7.3 ...
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bioRxiv - Cell Biology 2020Quote: Neonatal mouse cardiac myocytes (NMCMs) were isolated using the MACS cell separation kit (Miltenyi Biotec: Catalog #130-100-825 and #130-098-373). Briefly ...
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bioRxiv - Immunology 2021Quote: ... CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec #130-104-075) and then mixed with gp33-pulsed B cells at a ratio of 1:1 and incubated for indicated times at 37°C.
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bioRxiv - Immunology 2021Quote: B cells from spleens and lymph node of naïve mice were purified by magnetic separation using a pan-B cell isolation kit (Miltenyi Biotec #130-095-813), then incubated overnight with 30 μg/ml LPS (Sigma #L2630 ...
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bioRxiv - Immunology 2019Quote: ... Mouse total T and CD8+ native T cells were enriched from spleens and lymph nodes by negative selection using MACS system (Miltenyi Biotec, Auburn, CA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Ly6B.2+ and F4/80+ cells were isolated from splenocytes prepared as described above by immunomagnetic selection using Miltenyi isolation reagents (Miltenyi Biotec, Bergisch Gladbach). BMDMs ...
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bioRxiv - Cancer Biology 2021Quote: ... Freshly resected tumor tissue was dissociated into single cell suspensions using a tumor dissociation kit (130-096-730, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... were isolated from the spleen of naive Rdh10f/f Cd4Cre Rag1-/- OT-I CD45.1+ CD45.2+ (Rdh10CKO) or Rdh10f/f Rag1-/- OT-I CD45.1+ (control) mice using the Pan T Cell Isolation Kit II (Miltenyi Biotec, Bergisch Gladbach, Germany). For the adoptive transfer of memory T cells ...
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bioRxiv - Neuroscience 2020Quote: ... the cell suspension was incubated with the OPC MACS cultivation medium (MACS Neuro Medium containing MACS NeuroBrew-21 (Miltenyi Biotec, #130-093-566), GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... containing cell viability dye – APCH7 (cat. no. 65-0865-14, eBioscience, 1:5000) and anti-ACSA2 - PE (IH3-18A3, Miltenyi Biotec, 1:250), a specific marker of astrocytes ...