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Citations for Miltenyi Biotec :
2701 - 2750 of 3163 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and epithelial cells were selected using CD326 (EpCAM) magnetic beads (respectively 130-052-301 and 130-105-958, Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ expressing thymocytes were depleted using anti-CD4 and CD8 magnetic-activated cell-sorting (MACS) beads (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Astrocytes were isolated from the remaining cell suspension using the anti-ACSA-2 microbead kit (130-097-678, Miltenyi Biotec). The cell suspension was incubated with FcR blocking reagents followed by anti-ACSA-2 microbeads and placed on a magnetic MACS separator for positive selection ...
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bioRxiv - Cancer Biology 2023Quote: Naïve CD4+ T cells and nTregs were isolated from the spleens of healthy C57B6129SF1/J mice by magnetic separation using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec). The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution ...
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bioRxiv - Immunology 2023Quote: ... bacterial cells were washed again and re-suspended in PBS before they were analyzed with a MACSQuant cytometer (Miltenyi Biotec). C3 deposition analysis in age-matched WT ...
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bioRxiv - Genomics 2023Quote: All other solid organs were dissociated into single-cell suspensions using Miltenyi’s Liver Dissociation Kit (Miltenyi Biotec, 130-105-807) with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from mouse spleen and lymph nodes by magnetic bead sorting using the Naïve CD4+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Cecal and colonic lamina propria cells were extracted using the Lamina Propria Dissociation Kit and gentleMACS Dissociators (Miltenyi Biotec, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... and the supernatant was discarded while PMs were purified according to manufacturer’s instruction using a magnet purification kit by selecting F4/80+ cells (Miltenyi Biotec; Macrophage Isolation Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [29 ...
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were enriched in CD34+ cells using magnetic separation with the CD34 Microbead Kit (Miltenyi Biotec, San Diego, CA, USA) and further purified by flow cytometric sorting as described below.
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bioRxiv - Cancer Biology 2023Quote: ... macrodissected HCC nodules and control liver samples were dissociated as single-cell suspensions using the Liver Dissociation kit (Miltenyi Biotec) and the gentleMACS Octo Dissociator following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... the yeast cells were washed once with PBSB and incubated with 750 μL of Protein A microbeads (Miltenyi Biotec, 130071001) per 109 cells ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells from the spleen of Foxp3-mRFP mice were enriched using a CD4 negative selection kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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bioRxiv - Physiology 2024Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [14 ...
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bioRxiv - Genomics 2024Quote: ... we processed a fraction of the dissociated tumor for immune cell depletion using CD11b MicroBeads (Miltenyi Biotec #130-097-142) following manufacturer’s protocol on LS Columns ...
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bioRxiv - Immunology 2024Quote: Macrophages were isolated from lung single-cell suspensions using microbeads conjugated to anti-F4/80 antibodies according to the manufacturer’s protocol (Miltenyi Biotec). CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed twice with FACS buffer and analyzed using flow cytometry (MacsQuant Analyzer 10 flow cytometer, Miltenyi Biotec). The FlowJo™ software (BD Biosciences ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... lung single-cell suspensions were labeled with anti-CD4+ or anti-CD8+ magnetic beads and separated by autoMACS (Miltenyi Biotec), or CD4+CD8+ double positive cells purified from thymus single-cell suspensions by flow-cytometric sorting on FACS Aria (BD Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... hSS2 mouse tumors were harvested and digested to single cell suspension with the Miltenyi mouse tumor dissociation kit (Miltenyi Biotec). Approximately 5-10 million cells were crosslinked with 1% of formaldehyde ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then stained with fluorochrome-conjugated antibodies (anti-mouse-BV421-CD45) and anti-mouse-PE-O4 (Miltenyi Biotec)) for 40 min at 4°C to characterize oligodendrocytes (CD45-O4+) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then incubated with conjugated antibodies (FITC-CD11b, BD Biosciences, #557396; PE-Recombinant-ACSA2, Miltenyi Biotec, #130-116-244) for 45 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: We purified CD4+CD25-CD44lowCD62Lhigh naïve T cells obtained from the spleens of WT and Cars2+/– littermates with the Naïve CD4+ T Cell Isolation Kit II (130-093-227; Miltenyi Biotec) and an autoMACS Pro Cell Separator (Miltenyi Biotech) ...
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bioRxiv - Cancer Biology 2024Quote: Miltenyi bead separation was used according to the manufacturer’s instructions to purify CD8 T cells from the spleen tissue of WT FVB mice (#130-104-075, Miltenyi Biotec). Four subsets of tumor-infiltrated myeloid cells (CD45+CD11b+Ly6G- ...
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bioRxiv - Neuroscience 2024Quote: ... The cell dissociation procedure followed the guidelines of the Neural Dissociation Kit (T) (130-093-231, Miltenyi Biotec GmbH, Germany). After filtering with medium with 10 % (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... which were negatively selected using a magnetic cell sorting system (MACS) consisting of CD11b and CD45 MicroBeads and MS Columns (Miltenyi Biotec), were used as BM-MSCs ...
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bioRxiv - Cell Biology 2020Quote: ... Monocytes were isolated from PBMCs using a magnetic cell separation system with anti-CD14 mAb-coated microbeads (130-050-201; Miltenyi Biotec). CD14-positive monocytes were differentiated in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... Stem cell marker expression was validated in isolated cell lines by flow cytometry using anti-SSEA4-FITC (Cat. # 130-098-371, Miltenyi Biotec).
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes and microglia in the cell suspension were removed by using anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
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bioRxiv - Neuroscience 2021Quote: ... with either magnetic-activated cell sorting (MACS) using large cell columns and CD90 pre-conjugated to microbeads (#130-042-202 and #130-049-101, Miltenyi Biotec), fluorescence activated cell sorting (FACS ...
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bioRxiv - Immunology 2021Quote: Spleens of OT-I Hobit reporter × ROSA26-eYFP LT mice were isolated and subsequently CD8+ T cells were isolated by negative selection using MicroBeads (130-104-075, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Each tumor was dissociated into a single cell suspension with Miltenyi’s gentleMACS Dissociator according to the tumor dissociation kit protocol (Miltenyi Biotec, Germany). A small portion of the dissociated tumor cells was removed for profiling via flow cytometry ...
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bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Neutrophils were counted using an Invitrogen automated cell counter and the purity assessed by flow cytometry using FITC anti-CD16 and APC anti-CD15 antibodies (Miltenyi Biotec) as markers characteristic of human neutrophils.
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bioRxiv - Immunology 2019Quote: ... T cells were isolated by magnetic selection of CD4 or CD8 BM cells or splenocytes using the Miltneyi MACS system (QuadroMACS Separator, LS columns, CD4 [L3T4] MicroBeads, CD8a [Ly-2] MicroBeads; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: CD4+ and CD14+ cells were enriched from the PBMCs of 3 healthy donors via magnetic separation using the corresponding MicroBeads (Miltenyi Biotec). The CD14+ fraction was cryopreserved in liquid nitrogen ...
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bioRxiv - Immunology 2019Quote: Human monocytes were purified from peripheral blood mononuclear cells (PBMCs) from healthy donors by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). Monocytes were also purified from PBMCs from either HLA-A2 WT NOD2 donors or HLA-A2 homozygous mutant NOD2 Crohn’s patients ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were washed two times with PBS and the percentage of PI stained cells was determined using a MACSQuant VYB system (Miltenyi Biotec). Data analysis was performed using the FlowJo software.
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bioRxiv - Bioengineering 2019Quote: ... The monocytic cell fraction was isolated from the hPBMC fraction via negative selection using the MACS pan-monocyte isolation kit (Miltenyi Biotec) according to the supplier’s instructions ...
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bioRxiv - Immunology 2019Quote: B220+ B cells were recovered from bone marrow mononuclear cells from WT or CCR7−/− mice by negative selection using a MACS microbeads isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Fresh BM aspirates from MM patients were routinely depleted from malignant plasma cells using anti-CD138-coated magnetic beads (Cat. #130051301 Miltenyi Biotec) and used for RNA sequencing and functional assays.
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bioRxiv - Cancer Biology 2019Quote: ... whole lungs from mice bearing MDA-LM2 macrometastases (3 weeks post tail vein injection) were harvested and lysed in 1 ml Cell Lysis Buffer 2 (R&D) using the gentleMACS Dissociator (Miltenyi Biotec). As a control ...
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bioRxiv - Immunology 2021Quote: ... were co-transferred with CD3+ cell-depleted autologous PBMCs (2 × 106) isolated using CD3 Microbeads and the LD Column (Miltenyi Biotec) into 5- to 7-week-old female mice ...
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bioRxiv - Immunology 2021Quote: ... Untouched peripheral blood cDC1 and cDC2 (cDCs) and pDCs were obtained from PBMC after negative immunomagnetic separation with the Myeloid Dendritic Cell Isolation kit (Miltenyi Biotec) and the Plasmacytoid Dendritic Cell Isolation kit II (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2020Quote: ... the population of IFNγ secreting cells was identified using the IFNγ Secretion assay detection kit (PE) (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Peripheral neutrophils were isolated by negatively removing all contaminating cells using the MACSxpress Neutrophil Isolation Kit (Miltenyi Biotec, 130-104-434) and BD Pharm Lyse buffer (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... We suspended microbead pellets in additional sorting buffer (500 μL for 108 cells) and then loaded the suspended microbeads to a LS column (Miltenyi Biotec). We washed the column that was attached to a magnetic MidiMACSTM separator from Miltenyi Biotec for retaining CD3 magnetic microbeads with the sorting buffer three times to remove CD3-negative cells and then transferred the beads bound with T cells to a 15 mL falcon tube ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared from embryonic day E14.5 mice as described before [54] or from postnatal day 7-8 mouse brains using the MACS cell separation technology using the Neural Tissue Dissociation Kit and anti-O4 MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...