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Citations for Miltenyi Biotec :
3001 - 3050 of 3163 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The whole mouse brain was dissociated into a single-cell suspension using brain tissue dissociation kits following the manufacturer’s instructions (Adult Brain Dissociation Kit, Miltenyi Biotec, 130-107-677). Collected cells were then incubated with metal-conjugated antibodies in cell staining buffer (ProductMaxpar Cell Staining Buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The resected tumour samples were processed to a single-cell suspension using the commercial Tumour Dissociation Kit (30095929, Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
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bioRxiv - Bioengineering 2022Quote: ... MCs were enriched from PBMCs with manual magnetic activated cell separation (MACS) using the Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Immunology 2022Quote: ... B cells were depleted from OTII splenocytes by magnetic sorting using CD45R (B220) MicroBeads according manufacturer’s instructions (Miltenyi Biotec cat. 130-049-501). For experiments involving cell division analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Mobilized PBMCs or cord blood were enriched for CD34+ cells using magnetic beads according to manufacturer’s protocol (CD34 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany). CD3 depletion and CD34 enrichment were validated by flow cytometry unless specified otherwise ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs from sAML patients were isolated by Ficoll gradient extraction and CD34+ cells were isolated using magnetic enrichment (Miltenyi Biotec #130-100-453) and cultured overnight in SFEMII media (StemCell Technologies #09605 ...
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bioRxiv - Immunology 2020Quote: ... The analysis gating strategy was aiming to detect change in mean fluorescence intensity of cell surface CD303 expression (detected with Miltenyi Biotec clone AC144) on pDC (CD3-18-56-14-CD11C-DR+CD123+CD304+).
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bioRxiv - Neuroscience 2020Quote: ... following the manufacturer’s instruction followed by manual trituration using fire polished Silanized Pasteur pipette and filtration through 70µm cell strainer (Miltenyi Biotec, 130-095-823). Cells were pelleted at 300 × g ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Non-Neuronal Cells Biotin-Antibody Cocktail (Miltenyi Biotec, # 130-115-389) and incubated for 5 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec, Inc. Cat#130-094-363), CD19 and CD107a (BD Biosciences Cat#555800) ...
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bioRxiv - Bioengineering 2022Quote: ... hMCs were enriched from PBMCs with manual magnetic activated cell separation (MACS) using the Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, Cat. Nr.: 130-098-185). ADCC was measured using LDH release assay (Cytotoxicity Detection Kit (LDH ...
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bioRxiv - Immunology 2020Quote: ... and the CD4+ cells were isolated by positive immunomagnetic selection using MS columns and a mini MACS separator (Miltenyi Biotec, Bergish Gladbach, Germany) according to the instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Memory CD4+ T-cells were isolated from PBMCs of HIV-uninfected individuals by negative selection using magnetic beads (Miltenyi Biotec, Auburn, CA, USA), as we previously described (8 ...
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bioRxiv - Systems Biology 2021Quote: ... Single-cell suspensions were obtained by enzymatically digesting the excised tumors using the Tumor Dissociation Kit and gentleMACS C system (Miltenyi Biotec, Auburn, CA). In addition to following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Depletion of CD45+ cells in ovarian cancer ascites samples and NSCLC was performed using CD45 MicroBeads (Miltenyi Biotec, catalog no. 130-045-801) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... cell suspension with PBS supplemented with 0.5% BSA and 2 mM EDTA was applied to an MS column (Miltenyi Biotec, 130-042-201). After negative cells flow through the column ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...
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bioRxiv - Immunology 2020Quote: ... centrifuged and the cell pellet re-dispersed in 100-200 µl of PBS containing 0.5% BSA and the cell fluorescence intensity was measured using a MACSQuant VYB Flow Cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany). A minimum of 15 000 events were analyzed for each sample ...
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bioRxiv - Immunology 2020Quote: ... Microglia were purified from total collected cells using CD11b magnetic beads (Mitenyi Biotec #130-049-601) on columns (Miltenyi Biotec #130-042-201). The percentage of microglia among cells was determined by CD45 (Miltenyi Biotec #130-110-632 ...
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bioRxiv - Immunology 2021Quote: ... PDX tumor chunks were minced then enzymatically digested into single cells by using a tumor dissociation Kit (Miltenyi biotec Cat# 130-095-929). Mouse and human cells were separated through magnetic separation ...
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bioRxiv - Immunology 2022Quote: ... Single cells were depleted from both the CD4+ double positive (DP) and CD4+ single positive (SP) cells using a CD4+ (L3T4) positive selection kit (Microbeads; #130-117-043; Miltenyi Biotec, Gaithersburg, USA) flow-through (this contains both double-negative [DN] and CD8+SP cells) ...
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bioRxiv - Immunology 2022Quote: Untouched NK cells were isolated from frozen and thawed PBMCs using magnetic bead isolation according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany) and used immediately in co-culture assays ...
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bioRxiv - Microbiology 2022Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, cat. no. 130-098-185). Target cells were incubated with titrated concentrations of mAbs for 10 min and then with primary human NK cells at an effector:target ratio of 9:1 ...
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bioRxiv - Systems Biology 2022Quote: ... and T and B cells were depleted by using CD19 and CD3 MicroBeads (Miltenyi Biotec Cat#130-097-055 and #130-097-043) to enrich for myeloid cells ...
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bioRxiv - Molecular Biology 2022Quote: ... CD45- and CD45+ cells were separated using CD45 Microbeads with MACS MS magnetic columns according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were isolated from the bone marrow of donor mice via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Molecular Biology 2024Quote: ... Isolation of specific cell types was performed separately in male and female cultures An anti-CD11b microbeads antibody (Miltenyi Biotec, 130-049-601) and an anti-O4 microbeads antibody were used to isolate microglia and oligodendrocytes ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Bioengineering 2024Quote: ... were obtained after transduction of Jurkat T cells with a serial dilution of viral supernatants and transduction efficiency evaluated after 3-4 days by monitoring cell surface dNGFR expression using an anti-CD271-APC antibody (Miltenyi Biotec., Paris, France) or HLA-A*02 Dextramer-APC (Immudex ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells were magnetically isolated from spleens using CD8a+ negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-104-075). Purified OT-1 T-cells were resuspended in UCM ...
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bioRxiv - Cancer Biology 2024Quote: CD34+ HSPC were enriched from cord blood mononuclear cells (MNC) by magnetic selection using anti-CD34 conjugated magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec, 130-100-453). To obtain a sufficient cell number for ImageStream and Western blot analysis ...
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bioRxiv - Neuroscience 2022Quote: ... and processed into a single cell suspension according to the manufacturers’ protocol using the adult brain dissociation kit (Miltenyi Biotec 130-107-677). Cells were Fc blocked (Biolegend #101320 ...
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bioRxiv - Cancer Biology 2023Quote: CD3+ T cells were enriched from the BM of 30 MM patients using the Pan T cell isolation kit with MS columns (both Miltenyi Biotec, Bergisch, Germany). 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, cat. no. 130-098-185). Target cells were incubated with titrated concentrations of mAbs for 10 min and then with primary human NK cells at an effector to target ratio ranging from 7.75:1 to 9:1 ...
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bioRxiv - Cell Biology 2023Quote: Mouse primary NK cells were isolated from the spleens of mice (8 weeks old) by using the NK cell isolation kits according to the manufacturer’s instructions (Miltenyi Biotec #130-090-864). Primary NK cells were cultured in RPMI-1640 (Gibco ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... with 10 ml of sterile distilled water using a T-shaped inoculation spreader and spore suspensions were filtered through a 30-μm cell strainer (MACS, Miltenyi Biotec GmbH, Germany) to exclude mycelium ...
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bioRxiv - Immunology 2022Quote: B cells from PBMCs of uninfected controls or of COVID-19 convalescent individuals were enriched using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
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bioRxiv - Developmental Biology 2023Quote: The fetal lung samples were dissociated into single-cell suspension using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec; cat # 130-110-201) and 37C Multi_tissue_dissociation B program ...
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bioRxiv - Cancer Biology 2023Quote: Separations were performed on Control and EIF4G2KD HEC-1A cells using magnetic beads for CD133 (CD133 Microbead Kit, Miltenyi Biotec, 130-097-049), or CD44 (CD44 Microbeads Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T cells were then purified from total lymphocytes by negative selection according to the manufacturer’s instructions (Miltenyi Biotec, 130-096-543) and cultured in DMEM containing 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis analysis was evaluated by staining cells with Annexin V-FITC and Propidium Iodide (PI) (130-092-52, Miltenyi Biotec, Bergisch Gladbach, Germany), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... CD34+ HPCs were isolated using a CD34 MicroBead kit according to manufacturer’s instructions (magnetically activated cell sorting or MACS; Miltenyi Biotec, San Diego, CA). Pure populations of CD34+ HPCs were infected with TB40/E-WT or TB40/E-ΔUL138STOP HCMV (MOI = 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... αβ T cells were transduced with pMP71: γ-T2A-δ and incubated with a biotin-labelled anti-αβ TCR antibody (clone BW242/412; Miltenyi Biotec) followed by incubation with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were isolated from at least 5×106 PBMCs on the day of thaw by negative selection using the CD4+ T cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were mechanically and enzymatically dissociated with fire-polished Pasteur pipettes and the neural tissue dissociation kit with papain (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Erythrocyte lysis was terminated by washing the cells in the 10-fold volume of FACS buffer (autoMACS Running Buffer, Miltenyi Biotec, Bergisch Gladbach, Germany) and passed through a 30 µm cell strainer (BD Biosciences ...
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bioRxiv - Cancer Biology 2019Quote: ... the live cell fraction was subjected to a similar MACS based isolation by application of mouse MDSC isolation kit (Miltenyi Biotec, 130-094-538). Isolated cells were washed twice with cold MACS buffer and counted with a hemocytometer and diluted in cold PBS with 0.1% BSA and 2 mM EDTA at desired densities for Drop-seq.