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Citations for Miltenyi Biotec :
2901 - 2950 of 3163 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed twice and resuspended in 1mL of sorting buffer (up to 10⁸ cells) prior to magnetic separation on LS columns and the MACS Separator (Miltenyi Biotec). Columns were rinsed with 0.5mL of MACS buffer prior to loading the cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution; Miltenyi Biotec), TGF-β1 (5 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells from the sample were purified by magnetic bead separation using the CD4+CD62L+ isolation kit (Miltenyi Biotec). Cells were plated in 96-well plates coated with anti-CD3 antibody (5μg/ml ...
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bioRxiv - Immunology 2023Quote: ... cells were collected and centrifuged at 500 x g for 5 min at 4°C and CD8 T cells were isolated by magnetic sorting according to the manufacturer’s protocol (Miltenyi Biotec, Germany). To select transduced CD8 T cells ...
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC were differentiated into cells of all three germ layers using the StemMACS Trilineage Differentiation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly ...
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bioRxiv - Genetics 2023Quote: ... gentle and rapid generation of single-cell suspensions was achieved by using a modified version of the procedure of a mouse Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Neuroscience 2024Quote: ... Brain tissue was dissociated into a single-cell suspension by enzymatic digestion using the Adult Brain Dissociation Kit P (# 130-107-677, Miltenyi Biotec) and the gentleMACS™ Octo Dissociator (# 614 130-096-427 ...
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bioRxiv - Neuroscience 2024Quote: ... according to the manufacturer’s instructions including a red blood cell removal and a debris removal step (Adult Brain Dissociation Kit P, # 130-107-677, Miltenyi Biotec). Isolated cells were washed twice with D-PBS (# 14040-133 ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was immediately dissociated to single-cell suspension by enzymatic degradation and myelin removal using the adult brain dissociation kit and GentleMACS dissociator (Miltenyi Biotec.). The isolated tissues were placed in C-tubes ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells ...
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bioRxiv - Immunology 2023Quote: ... naive CD4+ T cells (CD4+CD25−CD62L+) were sorted from the spleen of WT C57Bl/6 mice using a commercially available kit (Miltenyi Biotec). Next ...
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bioRxiv - Neuroscience 2023Quote: ... a single cell suspension from the hippocampus and cortex was prepared using Neural Tissue Dissociation Kit (papain-based) (Miltenyi Biotec GmbH). After blocking with CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Biochemistry 2023Quote: ... and the flowthrough was enriched for CD4+ T cells by positive selection using anti-CD4 microbead antibodies (cat#130-045-101, Miltenyi Biotec). CD4+ T cell enrichment was confirmed by determining the percentage of CD3+/CD4+ cells via flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with FACS Intracellular Staining Perm Wash buffer and the following antibodies were added to the cells: anti-STAT1 pTyr701 (Miltenyi Biotec), and anti-STAT3 pTyr705 (eBioscience™) ...
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bioRxiv - Neuroscience 2023Quote: ... the suspension was counted and incubated with Anti-O4 MicroBeads at a concentration of 10 uL of beads per 1×107 cells (Miltenyi Biotec). After incubation ...
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bioRxiv - Physiology 2023Quote: ... cerebellum of WT and KIKO mice was dissociated to single-cell suspension using adult brain dissociation kit from MACS Technology and using GentleMACS (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then resuspended with 400 μl of buffer with 30 μl of CD326 (EPCAM) MicroBeads (130-105-958; Miltenyi Biotec) and incubated for 30 min at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... rested overnight at a density of 1×107 cells/mL in medium supplemented with 100 IU/mL IL-2 (Miltenyi Biotec). On day 7 ...
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bioRxiv - Bioengineering 2024Quote: ... and rested overnight in a density of 1×107 cells/mL in medium supplemented with 100 IU/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Bioengineering 2024Quote: Splenocytes were plated in 96-well U-bottom plates at 106 cells/well in RPMI and stimulated with 1 μg/mL of PepTivator Ovalbumin (Miltenyi Biotec). After 3 hours ...
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bioRxiv - Cancer Biology 2023Quote: Skeletal muscle myoblasts derived from WT and KO ADAMTSL1 mice were isolated using the Satellite Cell Isolation Kit MACS protocol (Miltenyi Biotec), as described previously34.
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bioRxiv - Immunology 2023Quote: ... Human blood samples were enriched for T cells using negative bead selection (StemCell) and stimulated in vitro in media containing a 1:100 titer of TransAct (Miltenyi Biotec), 5% FBS and 100 IU/mL of rhIL-2 ...
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bioRxiv - Physiology 2024Quote: ... The cells were then washed twice in stain buffer and applied to a magnetic column (cat. no.: 130-042-201, Miltenyi Biotec). The column was washed x 3 with stain buffer ...
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bioRxiv - Immunology 2024Quote: ... Cells were then resuspended in RPMI supplemented with 10%FBS and filtered through 30 µm MACS cell strainer (MACS Filters Miltenyi Biotec). The cells were counted to determine concentration and viability using a hemocytometer after which they were subsequently fixed for chromium fixed RNA profiling.
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bioRxiv - Immunology 2024Quote: ... The lung was perfused with 1X PBS and processed into single cell suspension using the mouse lung dissociation kit (Miltenyi Biotec). Blood was treated with RBC lysis buffer for five minutes ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401, Miltenyi Biotec). For CD206-positive (CD206+ ...
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bioRxiv - Plant Biology 2024Quote: ... the supernatants passed through a 40-μm cell filter and subsequently incubated with 100 µl anti-GFP magnetic beads (Miltenyi Biotec) at 4°C for one h ...
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bioRxiv - Bioengineering 2024Quote: iPS11 wild type (WT) and transgenic cell lines were grown in feeder-free conditions such as hiPSC Brew XF medium (Miltenyi Biotec) in Geltrex (Life technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting single cell suspension was incubated with anti-mouse CD11b (i.e., microglia-specific) magnetic MicroBeads (Miltenyi Biotec, #130–093–634) and microglia were positively selected for via column purification (Miltentyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed once with FACS buffer and then loaded onto a pre-wet LS column (Miltenyi Biotec, #130-042-401) on the MACS Multistand (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: CD14-positive monocytes were isolated from peripheral blood mononuclear cells (PBMCs) with CD14 microbeads and MACS LS column system (Miltenyi Biotec). PBMCs were obtained from the buffy coats of twelve healthy blood donors (eight males and four females ...
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bioRxiv - Molecular Biology 2024Quote: ... iPSCs expressing the pluripotency marker TRA-1-60 were sorted and enriched using magnetic-activated cell separation (MACS) technology (Miltenyi Biotec). Cells were cultured in neural induction medium (neurobasal medium supplemented with 1x neural induction supplement (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... the resulting pellet was resuspended in 100 μl of microbeads from the dead cell removal kit (Miltenyi Biotec, 130-090-101) and processed according to the commercial protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... CD4+ T cells were selected from resultant suspensions by labeling with bead conjugated anti-CD4 for enrichment by autoMACs (Miltenyi Biotec). Studies were approved by the Institutional Review Board at Baylor College of Medicine and informed consent was obtained from all patients.
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bioRxiv - Immunology 2024Quote: ... Human fetal thymus samples were HPCs were obtained from Ficoll-Hypaque–purified CB samples using the CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec), as described (38) ...
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bioRxiv - Immunology 2024Quote: ... CD4 and CD8 T cells isolated from spleen and lymph nodes were purified by incubating with anti-CD4 and CD8 microbeads (Miltenyi Biotec) and passing through LS magnetic columns following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... stimulated with IL-2 (R&D), and/or T cell differentiating cytokines and antibodies (Th1: anti-IL-4, IL-12 (Miltenyi Biotec), Treg ...
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bioRxiv - Microbiology 2024Quote: ... Harvested cells were centrifuged at 300×g for 5 min at 4°C and resuspended in red blood cell lysis buffer (#130-094-183, Miltenyi Biotec) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions containing the IEC fraction were diluted to 108 cells/mL in MACs buffer (0.5% BSA 2mM EDTA) before being processed with the Miltenyi Dead Cell Removal Kit (Miltenyi Biotec, Auburn, CA) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Cytotoxic CD8+ T cells were isolated from spleens of OT-I transgenic mice by magnetic labeling and separation (Miltenyi Biotec, Cambridge, MA). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... The brain tissues were cut into small pieces and dissociated into single cells using a modified version of the Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec, US). Myelin debris was removed using the Myelin Removal kit II (130-096-733 ...
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
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bioRxiv - Physiology 2021Quote: ... Flow cytometry analysis for assessment of dead cells was performed with the fixable viability dye Viobility 405/452 Fixable Dye (Miltenyi Biotec®). Viobility dyes react with amine groups of proteins and are non-toxic ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Harvested spleens from intramuscularly vaccinated mice were processed into single cell suspensions with a gentleMACS™ Octo Dissociator and Spleen Dissociation Kit (Miltenyi Biotec) with slight modifications to the manufacturer’s protocol ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Harvested lungs from intranasally vaccinated mice were processed into single cell suspensions with a gentleMACS™ Octo Dissociator and Lung Dissociation Kit (Miltenyi Biotec) according to manufacturer’s instructions including RBC lysis ...
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bioRxiv - Neuroscience 2021Quote: ... 3-week old heterogeneous neuronal cultures were enriched by a negative selection strategy using antibodies against surface markers CD44 and CD184 (recognizing NPCs, glial progenitors and astrocytes)35 using magnetic-activated cell sorting (MACS® - Miltenyi Biotec). After enrichment ...
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bioRxiv - Neuroscience 2020Quote: ... dissociated (see above) and the single cell suspension was used to enrich for OPCs by CD140a microbeads (Miltenyi Biotec, 130-101-502) according to manufacturer’s instructions ...