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Citations for Miltenyi Biotec :
2701 - 2750 of 3039 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were Fc blocked with α-CD16/32 (Biolegend ...
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bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
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bioRxiv - Immunology 2022Quote: ... Cell suspension was diluted with warm complete culture medium and incubated in a continuous motion using the MACSmix rotator (Miltenyi Biotec) for 45 min at 37°C (5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... Further disaggregation of iCCA tissue into a single-cell solution for sequencing was completed using the MACS tumor dissociation kit (Miltenyi Biotec) with the standard tough tumor protocol ...
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bioRxiv - Microbiology 2022Quote: ... Purified CD4+ T cells were characterized following counter-staining with anti-CD4-APC antibody (1:200) using flow-cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
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bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
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bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were dissociated into single-cell suspensions using the gentleMACS Dissociator (Cat. No. 130-093-235, Miltenyi Biotec, Bergisch Gladbach, Germany) with program “h_tumor_01” ...
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bioRxiv - Immunology 2022Quote: ... spleens were collected and processed into a single-cell suspension using a gentleMACS™ Dissociator (Miltenyi Biotec, North Rhine-Westphalia, Germany). RSV F–specific IFN-γ ELISpot assays were performed by seeding 500,000 splenocytes per well and stimulating overnight with a peptide pool representing the complete RSV A2 F protein sequence (JPT Peptide Technologies ...
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bioRxiv - Immunology 2022Quote: ... Cultures were activated with anti-CD2/CD3/CD28 coated beads at a bead to cell of ratio of 1:1 (Miltenyi Biotec). Cocultures were stained for viability with the LIVE/DEAD Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... whole splenocytes from wild-type and Slc29a3−/− mice were sequentially incubated with biotinylated anti-mouse CD3 (clone 145-2C11)/CD19 (clone 6D5)/NK1.1 (clone PK136)/Ly6G (clone aA8)/TER-119/erythroid cells (clone Ter-119) and Streptavidin MicroBeads (Miltenyi Biotec, Germany). The magnetically labelled cells were removed using autoMACS (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Treg and Tconv cells were enriched from PBMCs of patients with IBD and healthy donors using MACS CD4+CD25+ Regulatory T Cell Isolation Kit human (Miltenyi Biotec) for the co-culture experiments ...
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bioRxiv - Synthetic Biology 2024Quote: The mixed mouse primary neonatal cardiomyocyte cell culture was obtained using a neonatal heart dissociation kit (Miltenyi Biotec, 130-098-373) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Brain tissue was dissociated into a single-cell suspension by enzymatic digestion using the Adult Brain Dissociation Kit P (# 130-107-677, Miltenyi Biotec) and the gentleMACS™ Octo Dissociator (# 614 130-096-427 ...
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bioRxiv - Neuroscience 2024Quote: ... according to the manufacturer’s instructions including a red blood cell removal and a debris removal step (Adult Brain Dissociation Kit P, # 130-107-677, Miltenyi Biotec). Isolated cells were washed twice with D-PBS (# 14040-133 ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was immediately dissociated to single-cell suspension by enzymatic degradation and myelin removal using the adult brain dissociation kit and GentleMACS dissociator (Miltenyi Biotec.). The isolated tissues were placed in C-tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then resuspended with 400 μl of buffer with 30 μl of CD326 (EPCAM) MicroBeads (130-105-958; Miltenyi Biotec) and incubated for 30 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... the freshly collected whole lungs were dissected into single lobes to isolate single cells using a mouse lung dissociation kit (Miltenyi Biotec) and a gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2024Quote: ... Norway) centrifugation and used for CD4+ T cell isolation by negative selection with a commercial kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: We isolated single cells from the dissected mPFC sections using a neural tissue dissociation kit (Cat# 130-092-628, Miltenyi Biotec). After cell concentration quantified ...
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bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...
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2P-NucTag: on-demand phototagging for molecular analysis of functionally identified cortical neuronsbioRxiv - Neuroscience 2024Quote: ... and the homogenate was pipetted up and down to further break apart tissue before being passed through a 30 μm cell strainer (Miltenyi Biotec) and collected in a 15 ml conical tube.
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bioRxiv - Cancer Biology 2024Quote: ... The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50 ...
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bioRxiv - Immunology 2023Quote: ... were isolated from the spleen and lymph nodes of FoxP3-aPIK3CD mice using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec). Genomic DNA was isolated from these cells using the AllPrep DNA/RNA Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... spleen or bone marrow cells were stained for 30 min on ice with Anti-CD23 Magnetic Microbeads (130-098-784, Miltenyi Biotec) or Anti-F4/80 Magnetic Microbeads (130-110-443 ...
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bioRxiv - Immunology 2023Quote: Splenocytes or small intestinal IELs isolated from QFLTg or OT-1 mice were depleted of non-T cells using Pan T isolation Kit II (Miltenyi Biotec). The negatively selected T cells were then labeled with 5µM Cell Tracing Violet (CTV ...
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bioRxiv - Genomics 2024Quote: All tumours were labelled with Annexin specific MACS beads using the Dead Cell Removal Kit (Miltenyi Biotec Cat# 130-090-101) following the manufacturers’ instructions and dead cells were removed by passing the labelled cells through the autoMACS® Pro (Miltenyi ...
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bioRxiv - Cell Biology 2024Quote: ... to serve as a control (i.e., unstimulated cells) or interleukin- 1β (IL-1β; 50ng/mL; Miltenyi Biotec, Cat#130-101-682) plus interferon-γ (IFNγ ...
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bioRxiv - Immunology 2024Quote: ... staining and single-cell sorting of PvAMA1-specific IgG+ MBCs were performed as follows: B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec) and stained with mouse anti-human CD20 (PE-Cy5.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2mM EDTA) followed by addition of 10ml 1x red blood cell lysis (RBC) buffer (Miltenyi Biotec, Cat-Nr.: 130-094-183). Cells were incubated in RBC lysis buffer for 2 min at room temperature and then centrifuged at 600ɡ for 5 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was subjected to Magnetic Activated Cell Sorting (MACS) using CD11b magnetic microbeads (Order No. 130-093-634, Miltenyi Biotec) according to the manufacturer’s instructions and over LS columns after red blood cell lysis ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were incubated with the cells at 4°C for 30 minutes: anti-CD3 epsilon-VioBlue (Clone 17A2, Miltenyi Biotec), anti-TCR β-Alexa Fluor 488 (Clone H57-597 ...
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bioRxiv - Bioengineering 2024Quote: ... T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec, San Jose, CA). Next ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and labeled with 10 μL streptavidin MACS microbeads (Miltenyi Biotec, 130-048-102) (sufficient for 1e7 cells) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with Live/Dead dye and analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, San Diego, CA).
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bioRxiv - Cell Biology 2024Quote: ... The cells were stained with APC-efluo780 conjugated anti-c-kit antibody and c-kit+ cells were enriched using anti-APC paramagnetic microbeads (Miltenyi Biotec). Cells were stained with fluorophore-conjugated antibodies against lineage markers (CD2 ...
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bioRxiv - Cancer Biology 2023Quote: Skeletal muscle myoblasts derived from WT and KO ADAMTSL1 mice were isolated using the Satellite Cell Isolation Kit MACS protocol (Miltenyi Biotec), as described previously34.
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bioRxiv - Bioengineering 2024Quote: Splenocytes were plated in 96-well U-bottom plates at 106 cells/well in RPMI and stimulated with 1 μg/mL of PepTivator Ovalbumin (Miltenyi Biotec). After 3 hours ...
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bioRxiv - Immunology 2022Quote: ... to 1×108/ml and Ly6G+ cells were isolated according to the anti-Ly6G MicroBead Kit (#130-092-332, Miltenyi Biotec). The cells were labeled with biotinylated anti-Ly6G antibodies (anti-Ly6G-biotin ...
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bioRxiv - Neuroscience 2023Quote: Six organoids from each cell line were collected and processed using MACS papain neural tissue dissociation (Miltenyi Biotec #130-092-628) according to manufacturer’s protocol and resuspended in 0.22μm filter sterilized dPBS 0.04%BSA solution ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and the transfection efficiency as well as expression of the NSD2 constructs in HEK293 cells were evaluated based on the mVenus reporter by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and western blot using an anti GFP antibody (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... primary B cells were isolated from Rif1FH/FH and C57BL/6 (Rif1WT/WT) mice spleens using anti-CD43 MicroBeads (Miltenyi Biotec) and expanded in complete RPMI containing 5 µg/ml LPS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells were enriched on day 9 by depleting all CD43+ and all IgD+ cells with anti-mouse CD43 microbeads (Catalog:130-097-148, Miltenyi Biotec) and biotinylated anti-mouse IgD/anti-biotin microbeads (Catalog:130-090-485 ...
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bioRxiv - Immunology 2023Quote: ... Cells were magnetically labeled and separated according to the instructions of CD45 MicroBeads Human (order no. 130-045-801, Miltenyi Biotec). Then ...
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bioRxiv - Bioengineering 2023Quote: CD4+ and CD8+ T cells were then isolated from PBMCs by magnetic associated cell sorting (MACS) using the CD4+ or CD8+ human T cell isolation kit (130-096-533 or 130-096-495; Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2023Quote: ... through peripheral blood mononuclear cell isolation by Ficoll density gradient centrifugation and magnetic separation using CD14 MicroBeads (130-050-201, Miltenyi Biotec), according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... or Kdm6aflox/flox LckCre (UTXTCD) 8- to 12-week-old female donor spleens using the CD4+ T cell Isolation Kit II (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: SB CAR-T cells were generated as previously described from CD8+ or CD4+ bulk T cells purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec), activated with anti-CD3/CD28 beads according to the manufacturer’s instructions (Life Technologies ...