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Citations for Miltenyi Biotec :
7951 - 8000 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and MS columns (Miltenyi Biotec 130-042-201). Linneg Kit+ YFP+ cells were index sorted using a 70μm nozzle onto 384-well capture plates (10% Triton X-100 ...
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bioRxiv - Immunology 2020Quote: ... Samples were then run on the default lung_02 program on a gentleMACS Dissociator instrument (Miltenyi Biotec). Digestion was neutralized by adding 5mL 1% HI-FBS RPMI-1640 ...
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bioRxiv - Immunology 2020Quote: ... Cell suspensions were then processed with Mouse CD45 (TIL) MicroBeads (Miltenyi Biotec) to enrich for CD45+ TILs ...
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bioRxiv - Immunology 2020Quote: ... cells were isolated from WT C57BL/6 spleens using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec) and cultured as described previously (Shakya et al. ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T-cells were isolated from lymph nodes and spleens of OT-II TCR transgenic mice using negative selection with a mouse CD4+ T Cell Isolation Kit (Miltenyi Biotec). Splenocytes from C57BL/6 mice were T cell depleted by complement lysis of Thy1.2+ cells (clone J1J ...
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bioRxiv - Immunology 2020Quote: ... Cells were isolated using a mouse CD4+ T cell isolation kit (Miltenyi Biotec), and stimulated with 5 μg/ml plate-bound anti-CD3ε (BD Bioscience ...
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bioRxiv - Immunology 2020Quote: ... human peripheral blood mononuclear cells were activated with 50□ng/mL OKT3 (Cat. No. 130-093-387, Miltenyi Biotec) and harvested for retroviral transduction on day 2 ...
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bioRxiv - Immunology 2020Quote: TCR-deficient J.RT3-T3.5 cells were transduced with MEL5 TCR lentiviral particles and magnetically enriched using anti-murine TCRβ–PE (clone REA318) in conjunction with anti-PE MicroBeads (Miltenyi Biotec). MEL5 TCR+ J.RT3-T3.5 cells were then transduced with CD8αβ lentiviral particles ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were purified by CD14 selection using MACS magnetic beads (Miltenyi Biotec, Bergisch Glabach, Germany) and cultured in Roswell Park Memorial Institute medium-1640 (RPMI ...
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) using magnetic bead selection (Miltenyi Biotec, San Diego, CA, USA). To differentiate cells into macrophages ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 480 μL staining buffer before adding 120 μL anti-mouse IgG2a/b MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for an incubation period of 15 min ...
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bioRxiv - Immunology 2020Quote: ... Analyses were performed with MACSQuant Analyzer 10 and Flowlogic Software (both Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2020Quote: ... followed by labeling with anti-APC microbeads (Miltenyi Biotec). DP and CD8SP thymocytes were depleted by applying the “depleteS” program of AutoMACS Pro (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... DP and CD8SP thymocytes were depleted by applying the “depleteS” program of AutoMACS Pro (Miltenyi Biotec). Enriched CD4SP thymocytes were then stained with CD4-Pacific blue (RM4-5 ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 (10 ng/ml) (Miltenyi Biotec). PBMCs were stimulated for 3 days with 2 μg/ml PHA and if indicated ...
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bioRxiv - Immunology 2020Quote: ... γδ T cells were isolated from this suspension using the γδTCR+ T cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD3+ T cells were enriched by negative selection on a LD column and QuadroMACS separator (Miltenyi Biotec), before positive selection of γδ T cells using MS columns with an OctoMacs separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... before positive selection of γδ T cells using MS columns with an OctoMacs separator (Miltenyi Biotec). To yield a purer γδ T cell population ...
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bioRxiv - Immunology 2020Quote: ... IgA (FITC, clone IS11-8E10) (Miltenyi Biotec). Preformed conjugates for antigen-specific B cell sorting were made as described (46 ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Immunology 2020Quote: ... and purified using human CD34 Microbeads (Miltenyi Biotec). Isolated CD34+ cells were validated using flow cytometry (CD34-BV650 ...
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bioRxiv - Immunology 2020Quote: Intracellular staining with S100A8-PE (clone REA917, Miltenyi Biotec) was performed using the Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... iMS1 and iMono cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec; 130-104-075) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s instruction (Miltenyi Biotec). CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... Anti-SLAMF6 (REA) was obtained from Miltenyi Biotec (Bergisch Gladbach, Germany). Anti-IFN-γ (4S.B3 ...
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bioRxiv - Immunology 2020Quote: ... CD8 (BW135/80, Miltenyi Biotec), CD45RO (UCHL1 ...
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were positively selected from PBMCs using CD14 Microbeads according to the manufacturer’s instructions (Miltenyi Biotec). CD14+ cells were differentiated with 20 ng/mL of macrophage colony-stimulating factor (M-CSF ...
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bioRxiv - Immunology 2020Quote: Primary human CD14+ monocytes were positively selected from PBMCs using CD14 Microbeads according to the manufacturer’s instructions (Miltenyi Biotec) and differentiated for 3 days with 20 ng/mL M-CSF ...
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bioRxiv - Immunology 2020Quote: Monocytes were isolated from fresh blood packs (NHSBTS) using CD14 microbeads (Miltenyi Biotec) and the MACS system (Mitenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... The percentage of microglia among cells was determined by CD45 (Miltenyi Biotec #130-110-632) and CX3CR1 (Biolegend #149039 ...
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bioRxiv - Immunology 2020Quote: ... Microglia were purified from total collected cells using CD11b magnetic beads (Mitenyi Biotec #130-049-601) on columns (Miltenyi Biotec #130-042-201). The percentage of microglia among cells was determined by CD45 (Miltenyi Biotec #130-110-632 ...
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bioRxiv - Immunology 2020Quote: ... anti-Dectin-2 PE (REA1001) and anti-CD64 APC (REA286) were purchased from Miltenyi Biotec; rat anti-Dectin-2 (D2.11E4 ...
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bioRxiv - Immunology 2020Quote: ... Digested tissue was passed through a 100 µm filter (Miltenyi Biotec, Germany), washed ...
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bioRxiv - Immunology 2020Quote: ... Primary murine monocytes were isolated from C57BL/6J bone marrow using the Monocyte Isolation Kit (Miltenyi Biotec, 130-100-629) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and monocytes isolated using a negative selection kit (‘Pan Monocyte Enrichment’, Miltenyi Biotec, 130-096-537). Monocyte subsets were subsequently defined by flow cytometry after staining with anti-CD14 ...
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bioRxiv - Immunology 2020Quote: ... FITC anti-ITGA5 (Miltenyi Biotec, 130-110-592), APC anti-SIGLEC10 (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Immunology 2020Quote: Human monocytes were isolated from peripheral blood mononuclear cells from healthy donors (convention n°7828, Etablissement Français du Sang, Marseille, France) following CD14 selection using MACS magnetic beads (Miltenyi Biotec, Bergisch, Germany) as previously described (19) ...
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bioRxiv - Immunology 2020Quote: ... pro-B cells were positively selected from bone marrow using anti-CD19-labeled magnetic beads (Miltenyi Biotec) and were cultured for 24h hr in Opti-MEM supplemented with 15% FBS (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... The APC-conjugated anti-CD154 antibody was obtained from Miltenyi Biotec. PE-conjugated anti-GR-1 (RB6-8C5) ...
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bioRxiv - Immunology 2020Quote: ... Lung digestion mixes were placed on gentleMACS Octo Dissociator (Miltenyi Biotec) with a heater and digested at 37°C using default programme 37C_m_LDK_01 ...
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bioRxiv - Immunology 2020Quote: ... lymphocytes were counted using a MACSQuant flow cytometer (Miltenyi Biotec), using propidium iodide (PI ...
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bioRxiv - Immunology 2020Quote: ... CD34+ HSCs were isolated from the cord blood and perfused placental tissue by using the CD34 Microbead Kit (Miltenyi Biotec) and stored at −150°C ...
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bioRxiv - Immunology 2020Quote: ... Lung was collected in RPMI and was dissociated into single-cell suspension using the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) as per the company’s protocol ...
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bioRxiv - Immunology 2020Quote: CD14pos cells were isolated from peripheral blood mononuclear cells (PBMC) using CD14 micro-beads (Miltenyi Biotec) with the autoMACS Pro Separator according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... monocytes were purified from PBMC by using CD14+ microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were initially isolated from PBMC using CD4+ microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Immunology 2020Quote: ... followed by anti-biotin microbeads (Miltenyi Biotec). Labelled cells were added to MS columns (Miltenyi Biotec ...