Labshake search
Citations for Miltenyi Biotec :
7901 - 7950 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... utilizing CD90.2 microbeads (#130-121-278, Miltenyi Biotec). WT B6 ...
-
bioRxiv - Immunology 2020Quote: ... and the BM was depleted of T cells by autoMACS (Miltenyi Biotec, Bergisch Gladbach, Germany) utilizing CD90.2 microbeads (#130-121-278 ...
-
bioRxiv - Immunology 2020Quote: ... anti-CD40 (clone FGK45.5, Miltenyi Biotec GmbH, Gladbach, Germany), anti-CD80 (clone 16-10A1 ...
-
bioRxiv - Immunology 2020Quote: ... a negative selection with MHCII microbeads and a LS column (Miltenyi Biotec GmbH, Gladbach, Germany) was performed ...
-
bioRxiv - Immunology 2020Quote: ... dendritic cells were purified with two rounds of MACS magnetic separation using mouse CD11c microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Mouse (Miltenyi Biotec), according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2020Quote: ... Cells were rinsed once with media and then CD3+ T-cells were isolated via positive magnetic selection or pan T-cells were isolated via negative selection according to the manufacturer’s protocol (Miltenyi Biotec).
-
bioRxiv - Immunology 2020Quote: ... and the remaining adherent monocytes were cultured for 48h in the presence of IL-4 (300 U/ml) and GM-CSF (450 U/ml) (both from Miltenyi Biotec, Madrid, Spain) in X-VIVO 15 (BioWhittaker ...
-
bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...
-
bioRxiv - Immunology 2020Quote: ... anti-mouse IgG magnetic beads (Miltenyi Biotec) were added ...
-
bioRxiv - Immunology 2020Quote: Monocytes were purified from freshly thawed PBMC using CD14 antibodies conjugated to magnetic microbeads per the manufacturer’s recommendations (Miltenyi Biotec, San Diego CA). Magnetically bound monocytes were washed and eluted for collection ...
-
bioRxiv - Immunology 2020Quote: ... CD138 (Miltenyi Biotec, Bergisch-Gladbach, Germany), CD21/CD35 ...
-
bioRxiv - Immunology 2020Quote: ... 2 ng/ml interleukin 7 (IL7) (Miltenyi Biotec, Bergisch-Gladbach, Germany) and/or 1 μg/ml lipopolysaccharide (LPS ...
-
bioRxiv - Immunology 2020Quote: ... B cells were isolated by negative selection using the B Cell Isolation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany). The procedure was carried out following the instructions of the manufacturer ...
-
bioRxiv - Immunology 2020Quote: ... plates were blocked with 1% BSA in PBS solution (Miltenyi Biotec, Bergisch Gladbach, Germany) for two hours at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... interleukin 4 (IL4) (Miltenyi Biotec, Bergisch-Gladbach, Germany) was added to some wells at 10 ng/ml.
-
bioRxiv - Immunology 2020Quote: The Pan T Cell Isolation Kit II (Miltenyi Biotec, USA) was used for indirect isolation of T cells (CD3+CD8+ cytotoxic T cells and CD3+CD4+ T helper cells ...
-
bioRxiv - Immunology 2020Quote: ... on midiMacs type LS separation columns (Miltenyi Biotec; 130-042-401). Spleens were digested for 30 min at 37°C in digestion buffer [(RPMI 1640 supplemented 0.5 mg/ml collagenase D (Roche ...
-
bioRxiv - Immunology 2020Quote: ... and anti-mouse CD45-FITC before magnetic separation using anti-FITC microbeads (Miltenyi Biotec; 130-048-701) on midiMacs type LS separation columns (Miltenyi Biotec ...
-
bioRxiv - Immunology 2020Quote: ... Anti-PE and anti-APC beads and magnetized columns (both from Miltenyi Biotec) were used to enrich for tetramer-bound cells ...
-
bioRxiv - Immunology 2020Quote: ... by negative immunomagnetic selection (Miltenyi Biotec). NK cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Immunology 2020Quote: ... or recombinant IFN-α (Miltenyi Biotec), recombinant IL-12 (R&D systems and Peprotech ...
-
bioRxiv - Immunology 2020Quote: ... or recombinant IL-15 (Miltenyi Biotec) as described in the text and figure legends ...
-
bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
-
bioRxiv - Immunology 2020Quote: ... An additional dead cell removal step (Miltenyi Biotec) was conducted on hospitalized PBMC samples prior to aliquot for scRNA-seq ...
-
bioRxiv - Immunology 2020Quote: ... PolyPeptide group) were co-cultured with 105 OTII CD4+ T cells (purified with a CD4+ T cell isolation kit, Miltenyi Biotec) in RPMI medium (ThermoFisher ...
-
bioRxiv - Immunology 2020Quote: ... CD45+ hematopoietic cells were depleted using anti-CD45 magnetic beads by autoMACS with the depleteS program (Miltenyi Biotec). Total mTECs (EpCAM+UEA-1+Ly51lo) ...
-
bioRxiv - Immunology 2020Quote: ... anti-CD14 (TUK4, Miltenyi Biotec), anti-CD16 (3G8 ...
-
bioRxiv - Immunology 2020Quote: ... Plasmacytoid dendritic cells (pDCs) were isolated using a pDC isolation kit (Miltenyi Biotec). Splenocytes ...
-
bioRxiv - Immunology 2020Quote: ... (Miltenyi Biotec) by negative selection ...
-
bioRxiv - Immunology 2020Quote: ... Two magnetic-based cell sorting (MACS) isolation kits were used to purified either neutrophils (Miltenyi Biotec) and monocytes ...
-
bioRxiv - Immunology 2020Quote: ... and anti-PE microbeads (Miltenyi Biotec) as described previously[17] except for cell sorting ...
-
bioRxiv - Immunology 2020Quote: ... where mouse neutrophils were enriched from BM by positive magnetic selection with anti–CD11b microbeads (Miltenyi Biotec) before labelling with antibodies ...
-
bioRxiv - Immunology 2020Quote: ... and FlowLogic (Miltenyi Biotec).
-
bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec) and used directly for further differentiation into macrophages (Dominguez-Soto et al. ...
-
bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.
-
bioRxiv - Immunology 2020Quote: ... T cells were activated by injection of anti-CD3/CD28 beads (Miltenyi Biotec) at a ratio of 8:1 beads:T cell followed by injection of oligomycin (2μM ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cells were enriched from all samples using a CD4 TIL Microbeads kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... with the Aerosol Management System disabled or a MACSQuant Tyto (Tyto; Miltenyi Biotec). Aerosol samples were collected using a Cyclex-d impactor sampling cassette ...
-
bioRxiv - Immunology 2020Quote: ... All antibodies were purchased from Miltenyi Biotec, Invitrogen or BD Biosciences.
-
bioRxiv - Immunology 2020Quote: ... The numbers of cells that migrated to the lower well after 1 hr incubation were counted using a MACSQuant flow cytometer (Miltenyi Biotec). The percent migration was calculated by the numbers of neutrophils that migrated into the bottom chamber divided by the total number of neutrophils in the starting cell suspension and multiplying the results by 100 ...
-
bioRxiv - Immunology 2020Quote: ... The recovered cells were >98% CD14+ as determined by flow cytometry with anti-CD14 Ab (Miltenyi Biotec). The CD14 negative fraction (here PBL ...
-
bioRxiv - Immunology 2020Quote: ... Monocytes were purified by positive sorting using anti-CD14-conjugated magnetic microbeads (Miltenyi Biotec, Bergisch Gladsbach, Germany). The recovered cells were >98% CD14+ as determined by flow cytometry with anti-CD14 Ab (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2020Quote: ... Lungs were placed in GentleMacs C tubes (Miltenyi Biotec) with 2 mL Spleen Dissociation Medium (STEMCELL Technologies ...
-
bioRxiv - Immunology 2020Quote: ... (Miltenyi Biotec #130-126-700) at a 0.6 μM concentration (approx.1μg/ml) ...
-
bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cell fractions were isolated using the AutoMacsPro (Miltenyi Biotec). Enrichment of C7/C24 CD4+ T cells was confirmed by flow cytometry with a purity of ~95% ...
-
bioRxiv - Immunology 2020Quote: Murine CD4+ T cells were purified using the MACS CD4+ T cell negative selection kit (Miltenyi Biotec) and naïve CD4+ T cells from WT ...
-
bioRxiv - Immunology 2020Quote: Bulk polyclonal CD8+ T cells were isolated from the spleen and lymph nodes of WT or Dct-/- mice using negative magnetic enrichment (CD8a+ T cell Isolation Kit; Miltenyi Biotec). Enriched CD8+ T cells (typically ∼75-90% pure ...
-
bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...