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Citations for Miltenyi Biotec :
7501 - 7550 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and TNF-α-APC (Miltenyi Biotec) or granzyme B measurement with granzyme B-PE (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... IL-2-PE (Miltenyi Biotec) and TNF-α-APC (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... each sample was divided into two tubes for either cytokine detection with IFN-γ-FITC (Miltenyi Biotec), IL-2-PE (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... and TNF-α-APC (Miltenyi Biotec, cat #130-091-267).
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bioRxiv - Immunology 2020Quote: ... IL-2-PE (Miltenyi Biotec, cat #130-090-487) and TNF-α-APC (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ-FITC (Miltenyi Biotec, cat #130-090-433), IL-2-PE (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... and labelled cells were isolated using MS columns (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with magnetic anti-biotin beads (Miltenyi Biotec) and labelled cells were isolated using MS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... disassociated using the gentleMACS Octo Dissociator (Miltenyi Biotec). The C tubes were then incubated for 60 minutes at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... and in C Tubes (Miltenyi Biotec) disassociated using the gentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... CD14+ Monocytes were further isolated from PBMCs using anti-CD14 magnetic beads (130-050-201, Miltenyi Biotec). CD14+ monocytes were cultured in RPMI 1640 medium (10040CM ...
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bioRxiv - Cell Biology 2020Quote: ... Following astrocyte staining with ACSA-2-APC (Clone IH3-18A3, 1:200, Miltenyi Biotec), Hoechst to discriminate between cells and debris (10 µg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mm long and spanning the cortex depth but excluding the white matter) was microdissected and dissociated using the “Adult Brain Dissociation” kit from Miltenyi Biotec following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: For astrocyte enrichment the kit “Isolation and cultivation of astrocytes from adult mouse brain” (Miltenyi Biotec) was used according to the manual instructions ...
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bioRxiv - Immunology 2020Quote: Mouse monoclonal antibodies against human CD86 (clone FM95) and DC-SIGN (clone DCN47.5) were purchased from Miltenyi Biotec. Antibodies against human CD83 (clone HB15e ...
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bioRxiv - Immunology 2020Quote: ... DC differentiation was carried out over 6 days with 250 U/ml IL-4 and 50ng/ml GM-CSF (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Monocytes were purified by positive selection using the MACS CD14 isolation kit (Miltenyi Biotec) and cultured in 24 well plates ...
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bioRxiv - Molecular Biology 2020Quote: Primary cardiac cultures were isolated from P7 mice using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) using the gentleMACS homogenizer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... fluorophore-conjugated anti-HA antibody (either anti-HA-APC, catalogue number 130-098-404, or anti-HA-PE, catalogue number 130-092-257, both Miltenyi Biotec, Bergisch Gladbach, Germany) was added to obtain an 11X dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... human (Miltenyi Biotec) at 37°C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... purified mAbs were biotin-labeled using One-Step Antibody Biotinylation Kit (Miltenyi Biotec) following manufacturer recommendations and purified using 40K MWCO Desalting Column (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... filtering through a 50μm Cell Trics filter (Partec, Görlitz, Germany) and passing through an LD depletion column on a MidiMACS magnet (both from Miltenyi Biotec). The flow-through contained γδTc ...
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bioRxiv - Immunology 2020Quote: ... followed by anti-PE microbeads (Miltenyi Biotec), filtering through a 50μm Cell Trics filter (Partec ...
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bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... and IgG APC IS11-3B2.2.3 (Miltenyi Biotec). Data of labelled cells were acquired using a Beckman Coulter Gallios instrument and FlowJo ™ software (BD ...
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bioRxiv - Immunology 2020Quote: ... and CpG ODN2006 (final concentration 1 μg/ml, Miltenyi Biotec) were added ...
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bioRxiv - Cell Biology 2020Quote: ... by positive selection with anti-CD4 microbeads (Miltenyi Biotec Inc., Auburn, CA).
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, Cat. Nr.: 130-098-185). ADCC was measured using LDH release assay (Cytotoxicity Detection Kit (LDH ...
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bioRxiv - Immunology 2020Quote: ... with a GFP-expressing mRNA plasmid (Miltenyi Biotec) and a second plasmid encoding either full length gB from genotypes 1 ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... TCRβ+CD4+CD138− cells were isolated from TCRβ+CD138− cells using the CD4 (L3T4) MicroBeads (Miltenyi Biotec), and unbound cells were identified as TCRβ+CD8+CD138− cells.
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bioRxiv - Immunology 2020Quote: ... and TCRβ+CD138+ and TCRβ+CD138− cells were further separated with anti-PE magnetic MicroBeads (Miltenyi Biotec, Auburn, CA). After three washes with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were resuspended in complete medium (RPMI ...
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bioRxiv - Cell Biology 2020Quote: ... FcR Blocking Reagent mouse (130-092-575) was purchased from Miltenyi Biotec GmbH Bergisch Gladbach ...
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bioRxiv - Cell Biology 2020Quote: ... prior to separation into CD34+ and flowthrough (CD34−) cells using magnetic beads (CD34 Microbead Kit: Miltenyi Biotec, 130-046-702).
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bioRxiv - Immunology 2020Quote: ... MHCII and CD11C positive cells using magnetic beads (Miltenyi Biotec); 5 ×105 cells were plated onto the confluent endothelial cells for 16 h at 37°C ...
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bioRxiv - Genomics 2020Quote: ... dissociated cells were filtered through 30 µm cell strainers (Miltenyi Biotec 130-041-407). Cells were counted using an automated cell counter and 12 million cells were fixed and permeabilized using equal volumes of 1% paraformaldehyde and permeabilization buffer (DPBS ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... Cells were resuspended in MACS Quant running buffer (Miltenyi Biotec) and red blood cells were lysed using RBC Pharm Lyse (BD) ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... A red blood cell lysis step was performed by incubating cells for 2 minutes with 1x Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were washed again and then cell viability was measured using the Nexcelom Cellometer K2 Fluorescent Viability Cell Counter and the ViaStain AOPI Staining Solution (Nexcelom) ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... Tumor suspensions were filtered through a 70 µm MACS Smart Strainers (Miltenyi Biotec) and washed with PBS containing 0.5% BSA and 2 mM EDTA ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec). The “37C_m_TDK_2” and “m_imptumor_01” programs were used for the primary and secondary gentle MACS dissociation programs ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... Tumor tissue was minced and dissociated using the mouse Tumor Dissociation Kit (Miltenyi Biotec) and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... and the tissue was stored overnight at 4°C in MACS Tissue Storage Solution (Miltenyi Biotec). Tumor tissue was minced and dissociated using the mouse Tumor Dissociation Kit (Miltenyi Biotec ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... Tumors were placed into MACS tissue storage solution buffer (Miltenyi Biotec) on ice packs and shipped overnight for scRNA-seq processing.
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bioRxiv - Immunology 2020Quote: ... influenza A/California/04/2009(H1N1) NP (PepTivator, Miltenyi Biotec), influenza A/California/08/2009(H1N1 ...
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bioRxiv - Immunology 2020Quote: ... A pool of CD4+ and CD8+ T cells was isolated using CliniMACS CD4 and CD8 GMP MicroBeads (Miltenyi Biotec, Germany). GMP-grade lentiviruses ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR-positive transduced T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... truncated LNGFR (ΔLNGFR) was amplified from pMACS-ΔLNGFR (Miltenyi Biotec) and cloned into the construct with a P2A sequence immediately downstream of the EF-1α promoter to generate pLV-EF-1α-ΔLNGFR-P2A-CD19 CAR vector ...
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bioRxiv - Immunology 2020Quote: ... and the CD4+ cells were isolated by positive immunomagnetic selection using MS columns and a mini MACS separator (Miltenyi Biotec, Bergish Gladbach, Germany) according to the instructions provided by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... using a gentleMacs dissociator (Miltenyi Biotec). The lungs were then incubated for 30 min at 37°C and then further homogenized a second time with the gentleMacs ...