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Citations for Miltenyi Biotec :
7801 - 7850 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Mitochondria were further purified by affinity purification using anti-TOM22 microbeads (Miltenyi Biotec) as per the manufacturers protocol with several modifications ...
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bioRxiv - Cell Biology 2020Quote: ... CHIR99021 (0.9 μM or 1 μM, Miltenyi Biotec), SB431542 (10 μM ...
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bioRxiv - Cell Biology 2020Quote: ... cell selection for C-kit positive cells was performed with a CD117 antibody (Cat # 120-099-672, Miltenyi Biotec, Germany) in a Miltenyi Mini Macs System ...
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bioRxiv - Cell Biology 2020Quote: ... in combination with the gentleMACS™ Octo Dissociator (Miltenyi Biotec, 130-095-937) for the automated dissociation of tissues using standard tissue dissociation programs (program sequence ...
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bioRxiv - Cell Biology 2020Quote: ... cut into small pieces and digested using the Neonatal Heart Dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 U/mL leukemia inhibitory factor (LIF, Miltenyi Biotec); ‘2i+LIF’ was made of 50% Neurobasal medium (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... TOX APC (Cat # 130-118-474) was from Miltenyi Biotec (Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2020Quote: ... CD14-PE Vio 770 mAb (clone REA599, Miltenyi Biotec), IgG-Alexa Fluor-647 mAb (Cohesion Biosciences ...
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bioRxiv - Genetics 2020Quote: ... M-CSF (premium grade; Miltenyi Biotec) 5000U/ml ...
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bioRxiv - Immunology 2020Quote: ... moDCs were obtained by isolating monocytes from PBMCs using CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany), following Miltenyi Biotec’s protocol for generation of moDCs ...
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bioRxiv - Immunology 2020Quote: ... Tissue was dissociated into single-cell suspensions with enzymic digestions using a gentleMACS dissociate per protocol (Miltenyi Biotec). Single-cell suspensions were cryopreserved (10% DMSO ...
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bioRxiv - Genomics 2020Quote: ... The dissected cortex was dissociated using a papain-based neural dissociation kit (Miltenyi Biotec, Cat. N: 130-092-628) according to the manufacturer protocol with minor modifications ...
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bioRxiv - Immunology 2020Quote: Analysis of all flow cytometry data was performed using either MACSQuantify (Miltenyi Biotec) for cell counts ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMCs were then washed in PBS and counted on MACSQuant10 Analyzer (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Positive enrichment of GL7-expressing cells was performed using MACS LS columns (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Germinal center B cells were enriched by staining cells with GL7-PE followed with anti-PE magnetic beads (0.5 uL/107 cells, Miltenyi Biotec). Positive enrichment of GL7-expressing cells was performed using MACS LS columns (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Labelled cells were added to MS columns (Miltenyi Biotec) and positively selected cells (CD154+ ...
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bioRxiv - Immunology 2020Quote: Pools of lyophilized peptides covering the immunodominant sequence of the spike glycoprotein and the complete sequence of the membrane glycoprotein of SARS-CoV-2 (15-mer sequences with 11 amino acids overlap) were obtained from Miltenyi Biotec (Thieme et al., 2020) resuspended and stored according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... PBMCs from 48 healthy individuals (samples taken before the SARS-CoV-2 outbreak) were enriched for CD19+ cells with CD19-microbeads (Miltenyi Biotec). For each individual ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from PBMCs using CD19-microbeads (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... CD137-expressing CD4+ memory T cells cells were positively selected by staining with biotin-conjugated CD137 antibody (clone REA765; Miltenyi Biotec) followed by anti-biotin MicroBeads and applied to a new MS column ...
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bioRxiv - Immunology 2020Quote: ... centrifuged and the cell pellet re-dispersed in 100-200 µl of PBS containing 0.5% BSA and the cell fluorescence intensity was measured using a MACSQuant VYB Flow Cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany). A minimum of 15 000 events were analyzed for each sample ...
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bioRxiv - Immunology 2020Quote: Protein A beads and µ columns were from Miltenyi Biotec Ltd. ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T Cell Isolation Kit was from Miltenyi Biotec Ltd. ...
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bioRxiv - Immunology 2020Quote: ... monocytes were purified from human PBMC by using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
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bioRxiv - Immunology 2020Quote: ... NK cells were then isolated from PBMCs by negative selection according to manufacturer’s instructions (Miltenyi Biotec). For control of NK cell purity ...
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bioRxiv - Immunology 2020Quote: ... and activated with T Cell TransAct (Miltenyi Biotec); 36-48 h after activation ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were purified from PBMC via human CD8 MicroBeads (Miltenyi Biotec) and activated with T Cell TransAct (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... then filtered through 0.45- and then 0.22-micrometer molecular filters (Miltenyi Biotec) and finally ultracentrifuged twice at 100,000g for 70 minutes at 4°C [4] ...
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bioRxiv - Immunology 2020Quote: ... and activated with T Cell TransAct (Miltenyi Biotec, 130-111-160). After 36-48 h ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were purified from PBMC via human CD8 MicroBeads (Miltenyi Biotec, 130-045-201) and activated with T Cell TransAct (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... total PBMC were enriched in CD14+ monocytes using the human CD14 microbeads (Miltenyi Biotec) for positive magnetic selection according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... pooled cells were incubated with FcR blocking reagent (Miltenyi Biotec) for 10 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Pure monocytes (MO) were then isolated from PBMCs by positive selection with magnetic CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). Purified monocytes were resuspended in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2020Quote: ... cells were loaded onto two LS columns (Miltenyi Biotec) for magnetic enrichment of CD172a expressing cells ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of anti-APC-conjugated microbeads (Miltenyi Biotec) were added and incubated on ice for 30 min ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2020Quote: ... were diluted 1:100 in Tyto running buffer (Miltenyi Biotec). Aerosol testing was performed on a FACSAria II (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec). Enriched cells were subsequently washed ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were incubated on ice for 20 minutes with B220 microbeads (Miltenyi Biotec,; 20 μL/1000 μL SM per mouse), washed with SM and enriched on an AutoMACS Pro ...
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bioRxiv - Cancer Biology 2020Quote: ... Leland Chung) and PC3-mm (RRID: CVCL_4885) were blocked with human FcR blocking reagent (1:100; Miltenyi Biotec) and subsequently incubated with anti-CD117/c-kit-APC (1:50 ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently incubated with anti-CD117/c-kit-APC (1:50; Miltenyi Biotec; RRID: AB_615056). Cells were fixed in 1% formalin and analyzed on a BD FACS Canto II running the FACS Diva Software (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... followed by magnetic separation on a MACS LS column (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... we enriched B cells by incubating PBMCs with anti-CD20 MicroBeads (Miltenyi Biotec) followed by magnetic separation on a MACS LS column (Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: Primary human CD14+ monocytes were isolated from the peripheral blood mononuclear cells of healthy donors using anti-CD14 magnetic beads (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Tissue samples were homogenized 2 × 55 sec in 1 ml sterile phosphate buffer saline (PBS) using a gentleMACS™ Octo Dissociator (Miltenyi Biotec). Total DNA from bacterial cells and infected tissues was purified using a GeneJet Genomic DNA Purification Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... using a C-Tube (Miltenyi Biotec), which allows dissociation of viable single cells from tissue samples ...
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bioRxiv - Microbiology 2020Quote: ... was homogenized in 1 ml PBS for 2 × 55 sec on a gentleMACS™ Octo Dissociator (Miltenyi Biotec) using a C-Tube (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: ... αhuman-IgG-PE (Miltenyi Biotec). αHis-PE (Miltenyi Biotec) ...