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Citations for Miltenyi Biotec :
7701 - 7750 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and in presence of 33 U/ml human IL-2 (premium-grade, Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: ... the cell pellet re-suspended in 20 μl of anti-PE magnetic-activated cell sorting (MACS) microbeads (Miltenyi Biotec) with 80 μL separation buffer and incubated in the dark at 4°C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C on a rotating MACSmix (Miltenyi Biotec, USA) for 60 min ...
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bioRxiv - Cell Biology 2020Quote: ... Collected brain hemispheres were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec) and brain microglia cells were isolated using CD11b microbeads and MACS sorting columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... and brain microglia cells were isolated using CD11b microbeads and MACS sorting columns (Miltenyi Biotec). Single cell suspensions were subjected to scRNA-seq at the National Cancer Institute single cell analysis Core facility ...
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bioRxiv - Cell Biology 2020Quote: ... was purified using magnetic-activated cell sorting to deplete the supernatant of CD45+ cellular debris (Miltenyi Biotec, Bergisch Gladbach, Germany)36,37.
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bioRxiv - Cell Biology 2020Quote: ... which were then dissociated with a tumor dissociation kit (Human) using a mechanical–chemical–mechanical cycle in a gentleMACS Dissociator (Miltenyi Biotec), according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-His-HRP (Miltenyi Biotec, Cat# 130-092-785).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Multiple myeloma cells were then isolated from WBCs using human CD138 affinity magnetic beads (Miltenyi Biotec; cat#130-093-062) following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL, Melbourne, Australia) followed by CD14+ MACS magnetic bead separation (Miltenyi Biotec, Sydney, Australia). The isolated monocytes were differentiated for 7 days in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Anti-IL-10 (REA842) and anti-IL-6 (REA1037, Miltenyi Biotec) were used for intracellular staining.
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bioRxiv - Immunology 2020Quote: ... and streptavidin-APC (Miltenyi Biotec, 130-106-792). Single CD19+IgG+RBD+ cells were sorted into a 96 well plate containing 4 µL lysis buffer (12 units of RNasin Ribonuclease Inhibitor (Promega ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Lungs were processed into single cell suspension using the mouse lung dissociation kit (Miltenyi Biotec). The following fluorochrome-conjugated antibodies were used for labelling the cells ...
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bioRxiv - Immunology 2020Quote: ... Subsequent enrichment was performed with anti-PE beads using MACS technology (Miltenyi Biotec, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and Prot_S (each at 2 μg/ml; Miltenyi Biotec). Negative control wells lacked peptides ...
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bioRxiv - Immunology 2020Quote: Mononuclear single cell suspensions from whole mouse spleens were generated using a gentleMACS tissue dissociator (Miltenyi Biotec) followed by 70 μm filtration and density gradient centrifugation using Fico/Lite-LM medium (Atlanta Biologicals) ...
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bioRxiv - Cancer Biology 2020Quote: ... B16F10 cell lines were transfected with pRP[CRISPR]-hCas9-U6>PDPN gRNA 1 plasmid (constructed and packaged by Vector-builder; vector ID: VB160517-1061kpr) before negative selection using MACS LD columns (Miltenyi Biotec), anti-mouse podoplanin-biotin antibody (50 µg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-biotin microbeads (Miltenyi Biotec, 130-090-485).
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bioRxiv - Physiology 2020Quote: ... After MACS sorting of CD8+ T cell using a negative selection kit (CD8+ T Cell Isolation Kit II, human, Miltenyi Biotec), cells were lysed for western blotting or stained for Autophagy flux assay as described below ...
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bioRxiv - Microbiology 2020Quote: ... one fourth of the right lung was homogenized using a MACS homogenizer (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Neutrophils were isolated with MACSxpress neutrophil isolation and MACSxpress erythrocyte depletion kits (Miltenyi Biotec, Inc., Auburn, CA) and suspended in RPMI 1640 (without phenol red ...
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bioRxiv - Immunology 2020Quote: ... following manufacturer instructions (Dead cell removal kit; Debris removal kit; Miltenyi Biotec) resulting in ~80% of purity and CD3+ T cells were resuspended in 1X PBS with 0.04% BSA ...
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bioRxiv - Biochemistry 2020Quote: PBMCs were prepared as described above and NK cells were subsequently enriched using the MACS human NK cell isolation kit (Miltenyi Biotec) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Samples for surface staining were incubated with human FcR Blocking Reagent (Miltenyi Biotec) together with PE-conjugated mouse anti-GLAST 1:15 (Miltenyi Biotec 130-095-821 ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was dissociated using Neural Dissociation Kit-P (Miltenyi Biotec) following the manufacturer’s instructions and the cells resuspended in 1 ml of growth medium (Neurobasal medium containing 2% B27 supplements ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was gently re-suspended in 3100 μl cold D-PBS and processed for additional removal of myelin and cell debris with Debris Removal Solution (Miltenyi Biotec, cat. no. 130-109-398), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... was used at 1:200 iDisco-processed hemibrains were imaged by light sheet microscopy (Miltenyi Biotec Ultramicroscope II) using their 4x objective ...
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bioRxiv - Immunology 2020Quote: Freshly collected human decidual tissues were trimmed into 1 mm3 piece by GentelMACS Dissociator (Miltenyi Biotec, 130-093-235, Germany) and digested twice for 30 minutes each at 37°C with 1.0 mg/ml type IV collagenase (Gibco ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Immunology 2020Quote: ... anti-CD11c (REA754, Miltenyi Biotec), anti-CD11b (M1/70 ...
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bioRxiv - Immunology 2020Quote: Human and mouse lung tissues were digested using the Tumor Dissociation Kit (Miltenyi Biotec) for 30 minutes at 37°C and 1,500 rpm ...
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bioRxiv - Immunology 2020Quote: For cultured human lung macrophages staining: anti-CD169 (7-239) and anti-CD11c (REA618, Miltenyi Biotec) were used for membrane staining ...
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bioRxiv - Cell Biology 2020Quote: ... human (130-091-379, Miltenyi Biotec), from the negative fraction of the CD14-positive fraction.
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bioRxiv - Cell Biology 2020Quote: ... human (130-050-201, Miltenyi Biotec). The positive fraction was used as CD14-positive monocytes ...
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bioRxiv - Immunology 2020Quote: ... and human M-CSF (100 ng/ml; Miltenyi Biotec) were added ...
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bioRxiv - Immunology 2020Quote: ... cells were harvested from femur and tibiae of 6–9 week-old CD11c-DTR or CD11c-DTR Rag2−/− SJL mice and depleted of T cells using anti-CD3 and anti-CD8 magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec). Wild type or Rag2−/− SJL mice were gamma-irradiated with 950 rads from a 60Co source ...
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bioRxiv - Immunology 2020Quote: ... from spleen or axillary and brachial lymph nodes of naive PLP TCR transgenic SJL mice using magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec). The cells were labeled with 3 μM CellTrace Violet (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... the cells were labeled with the IFN-γ Catch Reagent (IFN-γ Secretion Assay-Detection Kit, human Miltenyi Biotec) containing bispecific antibodies for CD45 and IFN-γ which was secreted by the stimulated target cells ...
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bioRxiv - Cell Biology 2020Quote: Primary cardiac fibroblasts and cardiomyocytes were isolated from the hearts of P2-P3 neonatal mice using a commercially available dissociation kit following manufacturer’s instructions (Miltenyi Biotec, 130-098-373). In brief ...
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bioRxiv - Developmental Biology 2020Quote: ... Feeder Removal Micro Beads (Miltenyi Biotec) were used for depletion of MEFs while co-culturing with rat spermatogonial stem cells for gene expression and protein studies ...
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bioRxiv - Developmental Biology 2020Quote: ... The separation of STO cells from rat spermatogonial stem cells was carried using the Magnetic activated cell sorting (MACS) from Miltenyi Biotec.
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bioRxiv - Cell Biology 2020Quote: ... The cells were then resuspended in wash buffer and incubated in MACS human FcR blocking solution (20μl/sample, cat nb. 130-059-901, Miltenyi Biotec) and primary antibodies against CD45-FITC (12μl/sample ...
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bioRxiv - Cell Biology 2020Quote: ... CD82-PE-vio770 (Cat nb. 130-101-302 10μl/sample, Miltenyi Biotec) and CD34-APC (20μl/sample ...
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bioRxiv - Cell Biology 2020Quote: ... The cell pellet was resuspended in freezing buffer (StemMACS, cat nb. 130-109-558, Miltenyi Biotec) and stored 1-3 weeks at −80.
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bioRxiv - Cell Biology 2020Quote: ... CD31-FITC (4μl/sample, Cat nb. 130-110-668, Clone REA730, Miltenyi Biotec), CD31-PerCP-Vio700 (4μl/sample ...
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bioRxiv - Cell Biology 2020Quote: ... CD31-PerCP-Vio700 (4μl/sample, Cat nb. 130-110-673, clone REA730, Miltenyi Biotec), CD90-PE (3.2μl/sample ...
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bioRxiv - Cell Biology 2020Quote: ... and primary antibodies against CD45-FITC (12μl/sample, Cat nb. 130-114-567, Clone 5B1, Miltenyi Biotec), CD31-FITC (4μl/sample ...
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bioRxiv - Cell Biology 2020Quote: ... Mechanical and enzymatic muscle digestion was then performed at 37°C on the gentleMACS with heaters (cat nb. 130-096-427, Miltenyi Biotec) for 60 min using a skeletal muscle digestion program (37C_mr_SMDK1).
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bioRxiv - Cell Biology 2020Quote: ... The muscle slurry was then transferred to C-tubes (cat nb. 130-093-237, Miltenyi Biotec, Lund, Sweden) containing 8ml wash buffer including 700 U/ml Collagenase II (lot 46D16552 ...