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Citations for Miltenyi Biotec :
5551 - 5600 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... an Inside Stain kit (Miltenyi Biotec) was used according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed for 20 min at RT with 250 μL of the Inside Fix solution then washed and stained in 100 μL of the Inside Perm solution containing PE anti-TNF (clone cA2, Miltenyi Biotec) and APC anti-IL-1β (clone REA1172 ...
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bioRxiv - Immunology 2021Quote: ... and APC anti-IL-1β (clone REA1172, Miltenyi Biotec) antibody at 1/50 for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions containing the IEC fraction were diluted to 108 cells/mL in MACs buffer (0.5% BSA 2mM EDTA) before being processed with the Miltenyi Dead Cell Removal Kit (Miltenyi Biotec, Auburn, CA) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and purified their T cells using the untouched isolation kit (Pan T Cell Isolation Kit II, mouse, 130-095-130, Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... the negative cells fraction were further selected for the CD8+ positive cells (CD8a+ T Cell Isolation Kit, mouse, 130-104-07, Miltenyi Biotec). For the tetramers binding reaction ...
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bioRxiv - Immunology 2021Quote: ... Samples were loaded on MACS column (Miltenyi Biotec) and T cells were isolated according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Bone marrows cells were purified with CD3+ T isolated kit (CD3ε MicroBead Kit, mouse, 130-094-973, Miltenyi Biotec). Splenic CD4+ and CD8+ cells were purified in two steps ...
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bioRxiv - Immunology 2021Quote: ... and processed twice on a GentleMacs using the lung program (Miltenyi Biotec, Köln, Germany) with 30 min incubation at 37°C in between ...
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bioRxiv - Immunology 2021Quote: ... lung tissue was homogenized by gentleMACS using the RNA_01 program (Miltenyi Biotec). Genomic DNA was removed by on-column DNase digestion using RNase-Free DNase set (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... The lungs were removed into RPMI and homogenized by gentleMACS using the RNA_01 program (Miltenyi Biotec, Köln, Germany). The lung supernatant was stored at −80°C ...
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bioRxiv - Immunology 2021Quote: ... treated with erythrocyte lysis buffer and CD45+ leukocytes were isolated using LS columns and CD45 MicroBeads (35 μL beads per sample, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... followed by subsequent Streptavidin MicroBeads (Miltenyi Biotec, UK) for magnetic separation ...
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bioRxiv - Immunology 2021Quote: ... The uncollected non-myeloid (CD11b-) cells were processed with the Pan T Cell Isolation Kit (Miltenyi Biotec, 130-095-130) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The cell suspension was first incubated with anti-CD11b MicroBeads (Miltenyi Biotec, 130-049-601), and the on-column CD11b+ cells were collected as the myeloid cells ...
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bioRxiv - Immunology 2021Quote: Tumor nodules collected from the lungs of KP mice at time of analysis were cut into 1mm pieces and placed in mouse tumor dissociation kit (Miltenyi Biotec) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... were isolated by gradient centrifugation and then enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638).
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Immunology 2021Quote: ... Tumors were minced and processed following the instructions for the human tumor dissociation kit (Miltenyi Biotec, 130-095-929). After the addition of enzyme mix in 5 ml null RPMI ...
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bioRxiv - Immunology 2021Quote: ... and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec). Anti-CD24 antibody-bound cells were then magnetically removed ...
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bioRxiv - Immunology 2021Quote: ... CD14+ cells were sorted with the MACS system (Miltenyi Biotec), according to the manufacturer instructions and seeded at 5×105 cells/mL in RPMI-1640 media with L-glutamine (Lonza ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were obtained by pre-enrichment using an “untouched” CD4+ T Cell Isolation Kit (Miltenyi Biotec) with subsequent flow cytometric analysis (CD4+ [clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... and human CD45+ cells were selected by magnetic cell sorting (Miltenyi Biotec). scRNAseq libraries were prepared with the 10x Genomics’ Chromium Single Cell 3’ Library & Gel Bead Kit v2 ...
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bioRxiv - Immunology 2021Quote: ... KIR2DL1 expressing cells were isolated by staining the entire NK cell population with anti-KIR1-PE antibody (Miltenyi Biotec) followed by magnetic separation using the EasySep™ human PE selection kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... Right superior and inferior lobes were homogenized in 1 ml sterile PBS using gentleMACS Octo Dissociator (Miltenyi Biotec). A 100 μl aliquot of homogenate was reserved for bacteria cfu assay ...
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bioRxiv - Immunology 2021Quote: ... Results were read using a MacsQuant 16 Flow Cytometer (Miltenyi Biotec) and LegendPlex’s online data analysis tool (Qognit) ...
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bioRxiv - Immunology 2021Quote: ... Using the autoMACS® Pro separation system (Miltenyi Biotec), APC-labeled CD8α+ cells were depleted ...
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bioRxiv - Immunology 2021Quote: ... anti-CD8α-APC-conjugated antibody and anti-APC microbeads (Miltenyi Biotec). Using the autoMACS® Pro separation system (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... streptavidin microbeads (Miltenyi Biotec, Germany) and biotin-conjugated mouse anti-CD43 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... or overnight with 1 μg/peptide per well of spike associated (Cat: 130-127-951, Miltenyi Biotec Peptivator) peptides at 37°C ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were isolated from at least 5×106 PBMCs on the day of thaw by negative selection using the CD4+ T cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: Fresh G-CSF mobilized peripheral blood cells from healthy donor 1 were obtained from Miltenyi Biotec (Auburn, CA). CD34+ HSPCs were isolated using CliniMACS® CD34 reagent (Miltenyi ...
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bioRxiv - Genetics 2021Quote: ... were placed on a MACS MultiStand (Miltenyi Biotec) and equilibrated with washing buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... Stem cell marker expression was validated in isolated cell lines by flow cytometry using anti-SSEA4-FITC (Cat. # 130-098-371, Miltenyi Biotec).
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bioRxiv - Developmental Biology 2021Quote: Whole bone marrow cells were enriched for cKit+ cells using CD117 MicroBeads and MACS LS Columns (Miltenyi Biotec 130-091-224 and 130-042-401) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... The μMACS columns (Miltenyi Biotec, Cat# 130-074-101) were placed on a MACS MultiStand (Miltenyi Biotec ...
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bioRxiv - Genetics 2021Quote: ... RNA samples were incubated with 100 μl of streptavidin-coated μMACS magnetic beads (Miltenyi Biotec, 130-074-101) for 90 minutes at 24°C under gentle agitations ...
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bioRxiv - Genetics 2021Quote: ... using a gentleMACS dissociator (Miltenyi Biotec Inc). Total RNA was isolated by the Genome Technologies core facility at The Jackson Laboratory using the TRIzol Plus RNA Purification Kit (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeded at a density of 19,000 cells/cm2 on Matrigel in mTeSR1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec). Upon reaching 95% confluence ...
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bioRxiv - Developmental Biology 2021Quote: ... Pooled E18.5 sutures were similarly processed and red blood cell lysis (Miltenyi Biotec, 130-094-183) was performed after pelleting filtered cells ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 μM SB431542 (Miltenyi Biotec), supplemented with 10 μM Y-27632 (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... on a gentleMACS dissociator (Miltenyi Biotec, 130-093-235) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue dissociation was performed using Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628) on a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: Using a GentleMACS Octo Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany), 20-30mg of tissue was dissociated in 700µL of TRK Lysis Buffer (Omega ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were isolated by negative selection with magnetic cell sorting (Miltenyi Biotec) and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron ...
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bioRxiv - Immunology 2021Quote: ... lungs were extracted and processed with a gentleMACS dissociator (Miltenyi Biotec) and placed in gentleMACS C tubes containing 2.5mL RPMI ...
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bioRxiv - Immunology 2021Quote: ... and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec), then stained with the I-A/E-APC antibody (MHCII ...
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bioRxiv - Immunology 2021Quote: ... The cell fraction was then depleted with human CD45 beads (100ul beads per 2×108 cells; Miltenyi Biotec) using autoMACS ...
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bioRxiv - Immunology 2021Quote: Naive CD4+ or CD8+ T cells were positively selected from single-cell suspensions prepared from spleen and lymph node by mechanical disruption using streptavidin MicroBeads according to manufacturer’s instructions (Miltenyi Biotec). Purified cells were activated in twelve-well plates coated with 0.1-10 μg/mL αCD3 (145–2C11 ...