Labshake search
Citations for Miltenyi Biotec :
5401 - 5450 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: Tregs were isolated from peripheral blood mononuclear cells (PBMCs) by negative selection of CD4 (Miltenyi Biotec, Bergisch Gladbach, Germany) followed by positive CD25 selection (Miltenyi Biotec ...
-
bioRxiv - Immunology 2022Quote: ... Lung tissue was then transferred into gentleMACS C tubes (Miltenyi Biotec) and homogenized using a gentleMACS Dissociator ...
-
bioRxiv - Immunology 2022Quote: ... program “Lung 02_01” (Miltenyi Biotec). The resulting homogenized tissue was filtered through a 100μm BD Falcon cell strainer and washed with R-10 media.
-
bioRxiv - Immunology 2022Quote: Treg and non-Treg cells were enriched using MACS CD4+CD25+CD127dim/− Regulatory T cell isolation Kit human (Miltenyi Biotec) from PBMCs of healthy donors and plated (1 × 105 cells per well ...
-
bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike PepTivator® protein pools (Miltenyi Biotec, Gladbach, GER) were used to test T cell responses against full spike proteome ...
-
bioRxiv - Immunology 2022Quote: ... Delta and Omicron pools were obtained from Miltenyi Biotec. (3 ...
-
bioRxiv - Immunology 2022Quote: ... Macrophages were enriched using a magnetic-based commercial F4/80 macrophage positive selection kit (Miltenyi Biotec, MD, USA; Supplemental 3B) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... anti-CD66abce-PE-Vio770 (clone TET2; 1 μL; cat. # 130-119-849) from Miltenyi Biotec; anti-NKG2A-APC (clone Z199 ...
-
bioRxiv - Immunology 2022Quote: ... CD45+ and CD45- cells from the LPCs cell fraction were sorted using MACS (Miltenyi Biotec) with CD45 microbeads ...
-
bioRxiv - Immunology 2022Quote: Epithelial cells were isolated from colon samples in the pre-digestion buffer using the Lamina Propria Dissociation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
-
bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were isolated by MACS using the mouse CD4+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... plus 200 U/ml IL-2 (Miltenyi Biotec, Bergisch Gladback, Germany) for 3 days ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... These samples were each added to 200 µL of filtered M9 salt solution inside the wells of a 96 well plate and BFP+ and YFP+ cellular events were quantified by flow cytometry using a Macsquant10 (Miltenyi biotec). The competition plates were then sealed with Breathe-Easier Sealing Film (Diversified Biotech) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... these control populations were measured after at 24 and 48 time periods using the FSC channel of a Macsquant10 (Miltenyi biotec). Flow cytometry was performed with the same setting used in pairwise fitness assays (see above) ...
-
bioRxiv - Cancer Biology 2022Quote: ... with a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427). Single cell suspensions in 0.04% BSA-PBS of cell lines ...
-
bioRxiv - Biochemistry 2022Quote: ... Stool was homogenized via gentleMACS Dissociator (Miltenyi Biotec, 130-093-235) for 3 cycles of 61 s on the “intestine” setting ...
-
bioRxiv - Biochemistry 2022Quote: ... samples were decanted into sterile gentleMACS C tubes (Miltenyi Biotec, 130-093-237) and topped up to a total volume of 10 mL using 1X PBS + 40% glycerol + 0.001% L-cysteine solution ...
-
bioRxiv - Bioengineering 2022Quote: ... and lungs were processed into single-cell suspensions with a gentle MACS™ Octo Dissociator and Mouse Lung Dissociation Kit (Miltenyi Biotec, #130-095-297) according to manufacturer protocols.
-
bioRxiv - Immunology 2022Quote: ... and FcR blocked (#130-059-901, Miltenyi Biotec) for 15min at RT ...
-
bioRxiv - Microbiology 2022Quote: ... counted on a MACSQuant VYB FACS (Miltenyi Biotec) and data analysed using FlowJo version 7.6.5 ...
-
bioRxiv - Microbiology 2022Quote: ... yolk sacs were homogenized using gentleMACS™ Octo Dissociator (Miltenyi Biotec, Germany) and 100 μL of serial dilutions at 10-2 ...
-
bioRxiv - Neuroscience 2022Quote: ... hRGC differentiation was monitored by tdTomato expression and cells were purified during days 45-55 using THY1.2 microbeads and magnetic activated cell sorting system (MACS, Miltenyi Biotec) as explained before19,22 ...
-
bioRxiv - Bioengineering 2022Quote: ... Murine FcR blocking reagent (Miltenyi Biotec) was used to block unwanted binding of antibodies to mouse cells expressing Fc receptors ...
-
bioRxiv - Cell Biology 2022Quote: ... 1% Anti-foam Y-30) in M-tubes (gentleMACS™ Tubes, Miltenyi Biotec). Organs were then mechanically homogenized using a gentleMACS Dissociator (Miltenyi Biotec ...
-
bioRxiv - Immunology 2022Quote: ... Tumor Dissociation Kit (Miltenyi Biotec, cat. No 130-096-730) has been used to prepare single cell suspensions according to the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with anti-mouse CD45 MicroBeads and subjected to negative depletion (Miltenyi Biotec Inc). Cells were either used fresh for ex vivo experiments or preserved in CryoStor® CS10 freezing media (STEMCELL Technologies) ...
-
bioRxiv - Bioengineering 2022Quote: ... and 50 μl of 50nm-magnetic-bead antibodies against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523) and incubated for 15 min at RT ...
-
bioRxiv - Bioengineering 2022Quote: ... and run through a column equipped with a magnetic separator (MiniMACS Separator; Miltenyi Biotec, 130-042-102). Once the buffer had finished running through the column ...
-
bioRxiv - Genomics 2022Quote: ... and the GentleMACS Dissociator (Miltenyi Biotec). RNA and plasmid DNA extraction ...
-
bioRxiv - Genomics 2022Quote: ... Liver tissues were homogenized by using M tubes (Miltenyi Biotec) and the GentleMACS Dissociator (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2022Quote: ... The cell solution was then filtered through a 70 μm cell strainer and separated with magnetic beads (mouse CD31, Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... OT-I T cells were purified from spleens with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec), activated and cultured as previously described (11).
-
bioRxiv - Immunology 2022Quote: ... CD14+ monocytes and CD19+ B cells were enriched from fresh PBMCs derived from blood donors (Swiss Red Cross Laboratory; ECCT: CE-3428) through positive immunoselection (130-050-201 and 130-050-301, respectively [Miltenyi Biotec, Bergisch Gladbach, Germany]) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
-
bioRxiv - Immunology 2022Quote: Whole mouse lungs were digested and single cell suspension was prepared using dissociation kit (Miltenyi Biotec, Auburn, CA) as per the vendor’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
-
bioRxiv - Immunology 2022Quote: ... or MACSQuant (Miltenyi Biotec).
-
bioRxiv - Immunology 2022Quote: ... anti-CD66b-APC (Miltenyi Biotec), 5 µg/ml anti-CD45-AlexaFluor 647 (Santa Cruz Biotechnology) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were washed twice with serum free medium prior to FACS analyses (MACSQuant Analyzer, Miltenyi Biotec, Bergisch Gladbach, Germany). A forward- and side-scatter gate was set to exclude dead cells with forward-scatter (FSC ...
-
bioRxiv - Cell Biology 2022Quote: The fluorescence intensities of the recovered cells were analyzed using a MacsQuant Analyzer flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: Mouse scars and human stimulated skin and scar samples were digested in Miltenyi Whole Skin dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) for 2.5h according to the manufacturer’s protocol and processed on a GentleMACS OctoDissociator (Miltenyi) ...
-
bioRxiv - Cancer Biology 2022Quote: ... or also isolated using mouse CD31 microbeads (130-097-418, Miltenyi Biotec) and MS column (130-042-201 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and MS column (130-042-201, Miltenyi Biotec) with magnetic separator for some experiments.
-
bioRxiv - Cancer Biology 2022Quote: ... Dead cells were removed using dead cell removal kit (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Cancer Biology 2022Quote: ... on gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer’s instructions with following modifications ...
-
bioRxiv - Cancer Biology 2022Quote: ... For tumor samples digestion was performed using tumor dissociation kit for human (130-095-929, MACS Miltenyi Biotec), mechanical dissociation using gentleMACSTM Dissociator running the gentleMACS program h_tumor_01 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and treated with Red Blood Cell Lysis Solution (130-103-183, MACS Miltenyi Biotec) and dead cell removal using the Dead Cell Removal Kit (130-090-101 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and dead cell removal using the Dead Cell Removal Kit (130-090-101, MACS Miltenyi Biotec) to confirm cell viability >85% with trypan blue staining (Invitrogen) ...