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Citations for Miltenyi Biotec :
5701 - 5750 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Immunology 2021Quote: ... in RPMI 1640 medium in M tubes (Miltenyi Biotec, Woking, UK). After centrifugation at 880 × g for 5 min ...
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bioRxiv - Immunology 2021Quote: ... or from enriched mouse lung CD4+ T cells (CD4 T cell isolation kit, Miltenyi Biotec, Auburn, CA, USA), using RNeasy Micro Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Data were acquired using MACS Quant Analyzer 10 (Miltenyi Biotec) and were analyzed with FlowJo 10.0 (FlowJo LLC ...
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bioRxiv - Immunology 2021Quote: ... human (Miltenyi Biotec, Bergisch Gladbach, NRW, Germany). Blood CD4+ T cells were purified with a Naïve CD4+ T cell isolation kit II ...
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bioRxiv - Immunology 2021Quote: Each tumor was dissociated into a single cell suspension with Miltenyi’s gentleMACS Dissociator according to the tumor dissociation kit protocol (Miltenyi Biotec, Germany). A small portion of the dissociated tumor cells was removed for profiling via flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes or lung leukocytes were stimulated in duplicates with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Immunology 2021Quote: Splenocytes or lung leukocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 24 h ...
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bioRxiv - Immunology 2021Quote: ... Piece of lungs (200-300 mg) were weighed and homogenized using gentleMACS Octo dissociator (Miltenyi Biotec) in RPMI 1640 medium in M tubes (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2021Quote: ... nsRNAs were bound to 100 μL of μMACS streptavidin microbeads (Miltenyi Biotec) for 90 min at room temperature and purification was performed using the μMACS streptavidin starting kit (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2021Quote: ... for 90 min at room temperature and purification was performed using the μMACS streptavidin starting kit (Miltenyi Biotec). Columns were first equilibrated with 1 mL of washing buffer (100 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... tissues were enzymatically digested using GentleMACS whole skin dissociation kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) according to the manufacturers protocol ...
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bioRxiv - Pathology 2021Quote: ... Human recombinant OSM was added at 100 ng/ml (Miltenyi Biotec). Cells were cultured for 14 days at 37°C in 5% CO2 atmosphere ...
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bioRxiv - Genomics 2021Quote: ... human (Miltenyi Biotec) protocol ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in MACs buffer (Miltenyi BioTec, Bergisch Gladbach, Germany), and Fc receptors were blocked with anti-mouse CD16/32 (24G2 ...
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bioRxiv - Immunology 2021Quote: ... I-Ek VioBlue (REA510) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Cells were analyzed with a BD FACSAria II ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Microbiology 2021Quote: ... were pooled and dissociation was done using a collagenase I and II and a DNase I enzyme mix (all from BioConcept) and the gentleMACS Octo Dissociator (Miltenyi Biotec). Following this mechanical and enzymatical dissociation ...
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bioRxiv - Developmental Biology 2021Quote: ... Differentiating c-KIT+ neonate spermatogonia cells were magnetically labeled with CD117 (c-KIT+) MicroBeads (Miltenyi Biotec 130-091-224) and isolated using MS columns (Miltenyi Biotec 130-042-201 ...
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bioRxiv - Developmental Biology 2021Quote: ... and isolated using MS columns (Miltenyi Biotec 130-042-201) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... the population of undifferentiated neonate spermatogonia cells were separated using CD90.2 (THY1.2+) MicroBeads (Miltenyi Biotec 130-121-278). Relative enrichment of cell populations was evaluated by STRA8 (c-Kit fractions ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspension was obtained by mechanical resuspension followed by filtration through a 40-μm-pore-size cell strainer and dead cel;ls were removed using Dead Cell Removal Kit (Miltenyi Biotec 130-090-101). Differentiating c-KIT+ neonate spermatogonia cells were magnetically labeled with CD117 (c-KIT+ ...
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bioRxiv - Cancer Biology 2021Quote: ... lungs were automatically digested in digestion buffer using gentleMACS octodissociator (Miltenyi Biotec). The digested lungs were strained with a 70μm strainer ...
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bioRxiv - Cancer Biology 2021Quote: ... Freshly resected tumor tissue was dissociated into single cell suspensions using a tumor dissociation kit (130-096-730, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... the biopsies were cut into 2-3mm pieces and dissociated using a human Tumour Dissociation Kit (Miltenyi Biotec, Surrey, UK) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1:10 CD61-PE-Vio770 (Miltenyi Biotec, 130102627) for 1h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... C-kit+ cells were enriched using CD117 microbeads (Miltenyi Biotec). GMPs were sorted into RPMI containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: c-Kit+ bone marrow cells were enriched using CD117 MicroBeads and MACS separation columns (Miltenyi Biotec), and cultured in OptiMEM I reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... The large airways were removed and approximately ~500mg of airway free tissue was collected in a gentleMACS C-tube (Miltenyi Biotec, Auburn, CA) and 5mL digest buffer was added and placed on a gentleMACS octo dissociator for programmed runs ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: ... anti-IgA-Viogreen (Miltenyi Biotec, 130-113-481).
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bioRxiv - Immunology 2021Quote: ... Lungs from infected mice were digested using the Miltenyi lung cell isolation buffer and dissociated via GentleMACS (Miltenyi Biotec, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The isolated T cells were then stimulated with IL-7 and IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany) at a concentration of 25μg/2L bag of TexMACS media with 3% human AB serum (Innovative Research ...
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bioRxiv - Immunology 2021Quote: CAR T cell manufacture was automated with the use of the CliniMACS Prodigy® device using the TCT software program and TS520 tubing set (Miltenyi Biotec, Bergisch Gladbach, Germany). The instrument setup and technical protocol were described by Zhu et al.40 ...
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bioRxiv - Immunology 2021Quote: ... and the cytokines IL-7 and IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany). Peripheral blood apheresis products were loaded into the machine and CD4 and CD8 T cells were isolated using CliniMACS CD4 reagent and CliniMACS CD8 reagent according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... anti-CD45RO and anti-CD14 magnetically tagged antibodies (MACS, Miltenyi Biotec, Auburn, CA) to deplete NK cells ...
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bioRxiv - Immunology 2021Quote: ... Tumors were dissociated by mincing and digesting with the Tumor Dissociation kit (Miltenyi Biotec) per the manufacturer’s instructions using a gentleMACS dissociator (program h_tumor_01 and h_tumor_2) ...
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bioRxiv - Immunology 2021Quote: ... S1 and S+ peptide pools (1 mg/ml each, Miltenyi Biotec). Purified αCD40 (0,5 μg/μl ...
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bioRxiv - Genomics 2021Quote: ... gentleMACS C tubes were then placed on the gentleMACS Dissociator (Miltenyi Biotec) and tissue was homogenized by running the program “m_spleen_01” one to three times ...
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bioRxiv - Genomics 2021Quote: ... gentleMACS M tubes were then placed on the gentleMACS Dissociator (Miltenyi Biotec) and tissue was homogenized by running the program “RNA_02” ...
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bioRxiv - Genomics 2021Quote: ... a single frozen biopsy was placed into a gentleMACS M tube (Miltenyi Biotec) containing 600 µL of 2x DNA/RNA shield (Zymo Research) ...
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bioRxiv - Immunology 2021Quote: ... a debris removal kit was used as per the manufacturer’s guidelines (Miltenyi Biotec, San Diego, CA). The final debris-free cell pellet was resuspended in 1000 μL of 2% FBS in RPMI medium for downstream cell sorting using flow cytometry.
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bioRxiv - Immunology 2021Quote: ... CD45-PE (MB4-6D6, Miltenyi Biotec, San Jose, CA), CD20-ECD (B9E9 ...
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bioRxiv - Immunology 2021Quote: CD8+ P14 cells were negatively enriched (using the CD8+ T cell isolation kit – Miltenyi Biotec) from WT (CD45.1/2+ ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2021Quote: ... anti-IgA-Viogreen (Miltenyi Biotec, 130-113-481).
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bioRxiv - Immunology 2021Quote: ... Primary human CD8+ T cells were negatively isolated from PBMC using Human CD8+ T Cell isolation Kits (Miltenyi Biotec). Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and anti-TCRαβ (clone BW242/412, Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were gated on (i ...