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Citations for Miltenyi Biotec :
5651 - 5700 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and PE (Miltenyi Biotec 130-102-243), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through a 40 μm cell sieve (Miltenyi Biotec) and analyzed by flow cytometry (FACSaria III ...
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bioRxiv - Neuroscience 2021Quote: Brains were imaged either on the Ultramicroscope II (LaVision Bio Tec) or Ultramicroscope Blaze (Miltenyi Biotec) light-sheet microscope with a 1.0X objective ...
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bioRxiv - Molecular Biology 2021Quote: ... The μMACS columns (Miltenyi Biotec, Cat# 130-074-101) were placed on a MACS MultiStand (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2021Quote: ... were placed on a MACS MultiStand (Miltenyi Biotec) and equilibrated with washing buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... and CD45 (Miltenyi Biotec 130-052-301) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... with recommended dilutions of antibodies against CD31 (Miltenyi Biotec 130-097-418) and CD45 (Miltenyi Biotec 130-052-301 ...
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bioRxiv - Molecular Biology 2021Quote: ... red blood cell lysis was performed using the Red Blood Cell Lysis Solution (Miltenyi Biotec) following the manufacturer’s protocol that includes the washing step.
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bioRxiv - Molecular Biology 2021Quote: ... and 10ng/mL IL1β (Miltenyi Biotec, Cat.130-093-895). At day 3 ...
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bioRxiv - Neuroscience 2021Quote: ... To remove myelin the cell suspension was incubated with Myelin Removal Beads (130-096-733, Miltenyi Biotec) (6µl/brain ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed with 0.5% BSA-PBS and transferred to MACS columns (30-042-401 and QuadroMACS Separator 130-090-976, Miltenyi Biotec). The cell suspension was preincubated with TruStain FcX Plus Antibody (BioLegend ...
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bioRxiv - Molecular Biology 2021Quote: ... endothelial (CD31+) and myeloid (CD45+) cells were sorted using the Magnetic Cell Isolation and Cell Separation kit (Miltenyi Biotec) with recommended dilutions of antibodies against CD31 (Miltenyi Biotec 130-097-418 ...
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bioRxiv - Molecular Biology 2021Quote: ... 4.7 mL titrated LIF (Miltenyi Biotec 130-095-777), and 1.3 mL Beta-mercaptoethanol 55 mM (Gibco 21985-023) ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... Human CD4+ T cells were isolated by positive magnetic selection using human CD4 MicroBeads kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Resting primary B lymphocytes (wild-type and Tp53bp1-/-) were purified from mouse spleens using anti-CD43 microbeads (Miltenyi Biotec). One million cells were stimulated to proliferate with a cytokine cocktail containing 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 femora and 2 humeri was harvested in PBS/2.5% FBS and pooled before lineage negative depletion using autoMACS Pro separator (Miltenyi Biotec). Cells were incubated with a lineage antibody cocktail (B220 ...
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bioRxiv - Molecular Biology 2021Quote: ... lineage negative BM cells were first depleted using mouse lineage cell depletion kit (Miltenyi Biotec) then applied to five-laser FACSAria II sorter (BD Biosciences).
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bioRxiv - Immunology 2021Quote: CD4+ cells were isolated from C7 Tg mouse spleens using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol and cultured in T cell media at 2 x 106 cells/mL with 1 μM ESAT-6 peptide pool (NIH BEI Resources Repository catalog no ...
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bioRxiv - Immunology 2021Quote: ... CD138 PE (all Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... tuberculosis Erdman using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Spleens of OT-I Hobit reporter × ROSA26-eYFP LT mice were isolated and subsequently CD8+ T cells were isolated by negative selection using MicroBeads (130-104-075, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... 0.5 μg/mL anti-CD40 mAb (Miltenyi Biotec) and CXCR5 antibody were added ...
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bioRxiv - Immunology 2021Quote: ... 0.5 μg/mL anti-CD40 mAb (Miltenyi Biotec) and CXCR5 antibody were added ...
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bioRxiv - Immunology 2021Quote: ... cell suspensions were counted and stained with anti-CD45 microbeads (Miltenyi Biotec) for 15 min on ice ...
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bioRxiv - Immunology 2021Quote: ... before negative selection using the AutoMACS (Miltenyi Biotec) system ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin-streptomycin (v/v) and 50 ng/ml M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) and seeded at a density of 5 x 105 cells per well of a 6-well plate ...
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bioRxiv - Immunology 2021Quote: ... Fluorochrome-conjugated antibodies were used for specific immunostainings with dilutions (in autoMACS Running Buffer, Miltenyi Biotec, Bergisch Gladbach, Germany) as listed in table 4 ...
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bioRxiv - Immunology 2021Quote: ... Cells were analyzed using an 8-color flow cytometer (MACSQuant10, Miltenyi Biotec, Bergisch Gladbach, Germany) and data analysis was performed with FlowJo 10.4 (FlowJo LLC ...
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bioRxiv - Immunology 2021Quote: ... and cells were collected in FACS buffer (autoMACS Running Buffer, Miltenyi Biotec, Bergisch Gladbach, Germany). Blood was collected in EDTA-treated tubes (Sarstedt ...
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bioRxiv - Immunology 2021Quote: ... Erythrocyte lysis was terminated by washing the cells in the 10-fold volume of FACS buffer (autoMACS Running Buffer, Miltenyi Biotec, Bergisch Gladbach, Germany) and passed through a 30 µm cell strainer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... 500U/ml Collagenase I) with a tissue-lyser (Gentle MACS, Miltenyi Biotec, Bergisch Gladbach, Germany) and digested enzymatically for 20-30 min at 37°C while continuously shaking in a water bath ...
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bioRxiv - Immunology 2021Quote: ... naïve B cells were isolated from the spleens of mice using the B cell isolation kit (Miltenyi Biotec: 130-090-862) and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final ...
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bioRxiv - Immunology 2021Quote: ... Donor B cells were magnetically enriched from pooled spleens of recipient mice using APC-conjugated anti-CD45.1 and anti-APC MicroBeads (130-090-855; Miltenyi Biotec), with the MACS system (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... and MACS system (Miltenyi Biotec), as described previously (Nojima et al. ...
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bioRxiv - Immunology 2021Quote: ... with the MACS system (Miltenyi Biotec). After enrichment ...
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bioRxiv - Immunology 2021Quote: ... CD45RA (T6D11, Miltenyi Biotec), CD38 (HIT2 ...
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bioRxiv - Immunology 2021Quote: ... Cells were restimulated with anti-CD2/anti-CD3/anti-CD28 (T cell activation beads, Miltenyi Biotec) to achieve a 1:1 bead/cell ratio in accordance with the manufacturer’s instructions for cell expansion ...
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bioRxiv - Immunology 2021Quote: ... monocytes were enriched by negative selection using a Pan Monocyte Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Next ...
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bioRxiv - Immunology 2021Quote: ... Biopsies were stored in buffer (MACS Tissue Storage Solution, Miltenyi Biotec) at 4°C and processed within 2 hours for cell isolation or PFA fixation.
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bioRxiv - Immunology 2021Quote: ... cut into 4 pieces and enzymatically digested by using the whole skin dissociation kit from Miltenyi biotec without enzyme P for 5 hours at 37°C under agitation (500 rpm) ...
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bioRxiv - Immunology 2021Quote: ... On day4 transduced cells were positively selected by magnetic assisted cell sorting (MACS) via the cell surface antigen CD90.1 (Thy1.1) using microbeads (Miltenyi Biotec: 130-094-523); the manufacturer’s protocol was adjusted by using 4x less reagents and by maintaining cells at room temperature ...
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bioRxiv - Immunology 2021Quote: ... T cell activation by peptide was assessed according to the expression of eGFP regulated by the NFAT promoter and analysed on a MACSQuant Analyzer 10 (Miltenyi Biotec). The acquired data was processed by FlowJo (version 10.6.2 ...
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bioRxiv - Immunology 2021Quote: ... dead cells were removed from our 24h activation sample (∼50% viability) using a MACS® Dead Cell Removal Kit (Miltenyi Biotec). In brief ...
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bioRxiv - Immunology 2021Quote: ... using the untouched CD4+ T cell isolation kit II (Miltenyi Biotec, Germany). More than 98% of purified cells were routinely CD4+ ...
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bioRxiv - Immunology 2021Quote: ... The lungs were placed in a GentleMacs C tube (Miltenyi Biotec, Bergisch Gladbach, Germany) with 2mL STEMCELL Spleen Dissociation Medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... B cells were purified by negative selection (Miltenyi Biotec, Cat#130-091-151) from PBMCs and stimulated with R848 (1 ug/ml) ...
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bioRxiv - Immunology 2021Quote: Tumor or metastatic tissue was thoroughly chopped with surgical scissors and transferred to gentleMACS C Tubes (Miltenyi Biotec) containing 20 uL/mL Liberase TL (5 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... gentleMACS C Tubes were installed onto the gentleMACS Octo Dissociator (Miltenyi Biotec) and incubated for 45 minutes according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Naive B cells were purified from PBMCs using the MACS Human B Cell isolation kit (Miltenyi Biotec) and incubated with 25nM of each SARS-CoV-2 probe (RBD-mFc-BV650 ...