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Citations for Miltenyi Biotec :
5801 - 5850 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The cells were passed in MS Columns (Miltenyi Biotec, Bergisch Gladbach, Germany), and only the ACSA-1 positives cells stay attached in the columns ...
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bioRxiv - Cancer Biology 2021Quote: Autologous patient derived T cells were isolated using CD3 MicroBeads (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... strained through a 70 μm cell strainer (Miltenyi biotec), counted ...
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bioRxiv - Cell Biology 2021Quote: ... Splenic B cells were isolated with the CD45R(B220) cell isolation kit (Miltenyi Biotec). The purity of B220 cells was >90% ...
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bioRxiv - Neuroscience 2021Quote: ... after with anti-PE microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany). The cells were passed in MS Columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... with the mouse lung dissociation kit (130-095-927, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... The lung tissue was cut into pieces and processed using a GentleMACS Octo Dissociator (program 37C_m_LDK_1, Miltenyi Biotec) with the mouse lung dissociation kit (130-095-927 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD133 and HER2 expression on the tumor cell lines were detected using anti-CD133 (130-098-826, Miltenyi Biotec) and anti-HER2 (BMS120Fl ...
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bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+ monocytes were collected in the resuspended cell suspension by incubating with anti-human CD14 microbeads (Miltenyi Biotec) for 15 mins at 4°C and separated using a MACS Pro Separator (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2021Quote: ... for 15 mins at 4°C and separated using a MACS Pro Separator (Miltenyi Biotec). Monocytes were cultured using Iscove’s Modified Dulbecco’s media supplemented with 10% human serum blood type AB ...
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bioRxiv - Cancer Biology 2021Quote: ... and stimulated with TransAct™ (Miltenyi Biotec). Batch 2 T cells were cultured in R10 Medium containing RPMI 1640 (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... and stimulated with particles coated with anti-CD3/anti-CD28 antibodies (Miltenyi Biotec) at a cell-to-bead ratio of 1:2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary human T cells were isolated from PBMCs by negative selection using the Pan T Isolation Kit (Miltenyi Biotec). Batch 1 T cells were cultured in IMDM Medium containing Icove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Biochemistry 2021Quote: Memory B cells were isolated from Peripheral blood mononuclear cells (PBMCs) derived from blood samples from COVID-19 convalescent patients using a Human Memory B cell isolation kit (Miltenyi Biotec, GE). Small pools of purified Memory B cells were seeded into 384-well plates on irradiated CD40L-expressing feeder cells for differentiation into plasma cells as described previously (41) ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were collected and their activation assessed using flow cytometry (MACS-Quant, Miltenyi Biotec). Specifically ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Bioengineering 2021Quote: ... and analyzed by flow cytometry using a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). FlowJo software (version 10.6.2 ...
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bioRxiv - Bioengineering 2021Quote: ... CD8+ T cells were isolated using a CD8a+ T Cell Isolation Kit (Miltenyi Biotec). For pMHC liposome activation in vitro ...
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bioRxiv - Bioengineering 2021Quote: ... Peripheral neutrophils were isolated by negatively removing all contaminating cells using the MACSxpress Neutrophil Isolation Kit (Miltenyi Biotec, 130-104-434) and BD Pharm Lyse buffer (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+-cells were isolated by magnetic activated cell sorting (MACS) with positive selection using CD14 MicroBeads (130-050-201; Miltenyi Biotec B.V. & Co. KG) from freshly isolated SVF or PBMCs according to supplier’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... an LS column (130-042-401; Miltenyi Biotec B.V. & Co. KG) was placed into the magnetic field of an QuadroMACS Separator (130-090-976 ...
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bioRxiv - Bioengineering 2021Quote: ... was placed into the magnetic field of an QuadroMACS Separator (130-090-976; Miltenyi Biotec B.V. & Co. KG) and primed by rinsing with 3 ml of MACS buffer ...
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bioRxiv - Bioengineering 2021Quote: ... 1:20 in autoMACS Rinsing Solution (130-091-222; Miltenyi Biotec B.V. & Co. KG). For degassing ...
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bioRxiv - Bioengineering 2021Quote: ... CD3/CD28-mediated activation of T cells was conducted by using 10 μl of T cell TransActTM (130-111-160; Miltenyi Biotec B.V. & Co. KG) according to manufacturer’s instructions (T cell activation medium) ...
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bioRxiv - Bioengineering 2021Quote: ... MACS buffer was prepared freshly before each isolation by diluting MACS BSA Stock Solution (130-091-376; Miltenyi Biotec B.V. & Co. KG) 1:20 in autoMACS Rinsing Solution (130-091-222 ...
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bioRxiv - Bioengineering 2021Quote: ... and the cell pellet was gently re-suspended in Red Blood Cell Lysis Solution (freshly prepared according to manufacturer’s instruction; 130-094-183; Miltenyi Biotec B.V ...
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bioRxiv - Immunology 2021Quote: ... and CD25 MicroBeads II (Miltenyi Biotec), respectively ...
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bioRxiv - Bioengineering 2021Quote: ... were individually mixed with NHS-activated MNPs (Miltenyi Biotec, #130-105-805) at a molar ratio of 10:1 and incubated at 4°C overnight with gentle mixing ...
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bioRxiv - Bioengineering 2021Quote: ... the conjugated MNPs were purified with a magnetic micro-column (Miltenyi Biotec, #130–042-701). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... 1% bovine serum albumin (BSA, Miltenyi Biotec, 130-091-376) and 5 ng/mL luteinizing hormone from human pituitary (LH ...
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bioRxiv - Bioengineering 2021Quote: ... 1% bovine serum albumin (BSA, Miltenyi Biotec, 130-091-376) and 5 ng/mL luteinizing hormone from human pituitary (LH ...
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bioRxiv - Bioengineering 2021Quote: ... 0.2% BSA (Miltenyi Biotec, 130-091-376), 1% penicillin/streptomycin (Sigma Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... and CD81-FITC (Miltenyi Biotec, Germany). EVs were incubated with antibodies for 60 min at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... and the labeled vesicles were analyzed by flow cytometry (MACSQuant Analyzer, Miltenyi Biotec, Germany).
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bioRxiv - Bioengineering 2021Quote: ... We washed the column that was attached to a magnetic MidiMACSTM separator from Miltenyi Biotec for retaining CD3 magnetic microbeads with the sorting buffer three times to remove CD3-negative cells and then transferred the beads bound with T cells to a 15 mL falcon tube ...
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bioRxiv - Bioengineering 2021Quote: ... we suspended pelleted PBMCs in the sorting buffer (80 μL for 107 cells) with CD3 magnetic microbeads (20 μL for 107 cells) from Miltenyi Biotec for 15 min at 4 °C according to the capacity of the microbeads ...
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bioRxiv - Bioengineering 2021Quote: ... We suspended microbead pellets in additional sorting buffer (500 μL for 108 cells) and then loaded the suspended microbeads to a LS column (Miltenyi Biotec). We washed the column that was attached to a magnetic MidiMACSTM separator from Miltenyi Biotec for retaining CD3 magnetic microbeads with the sorting buffer three times to remove CD3-negative cells and then transferred the beads bound with T cells to a 15 mL falcon tube ...
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bioRxiv - Bioengineering 2021Quote: ... mouse and rat (Miltenyi Biotec). Cardiomyocytes and non-cardiomyocytes were separated by Percoll gradient centrifugation ...
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bioRxiv - Bioengineering 2021Quote: ... A commercial Adult Brain Dissociation Kit (Miltenyi Biotec) was used to dissociate the cortex samples into individual cells by following the separation protocol from the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... incubated with anti-biotin microbeads (Miltenyi Biotec), placed on LS columns ...
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bioRxiv - Immunology 2021Quote: ... and separated into CD3ε+ and CD3ε- fractions according to manufacturer’s directions (Miltenyi Biotec). CD3ε+ and CD3ε- fractions were each fluorescently-sorted into four subpopulations based on surface marker expression shown in Figure 1 and Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... brain punches were dissociated using a neuronal tissue dissociation kit (Miltenyi Biotec, 130-092-628), applied on a 70-μm MACS smart strainer and washed with HBSS 1× ...
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bioRxiv - Neuroscience 2021Quote: ... cells were magnetically labeled with CD45 microbeads (Miltenyi Biotec, 130052301) and passed through a MACS MS column (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... CD45-fraction was collected and magnetically labeled with CD31 microbeads (Miltenyi Biotec, 139097418) and then passed through MACS MS column to positively select CD31+ cells ...
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bioRxiv - Neuroscience 2021Quote: ... and passed through a MACS MS column (Miltenyi Biotec, 130-042-201) to proceed to negative selection of CD45 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Endothelial cells were enriched from PFC punches by using MACS according to the manufacturer’s protocol (Miltenyi Biotec). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... and magnetic separation was done using Multi-24 Column Blocks and the MultiMACS Cell24 Separator Plus (130-095-692 and130-098-637, Miltenyi Biotec). O4+ cells were collected in BSA 0.5 % in PBS solution and counted ...
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bioRxiv - Neuroscience 2021Quote: ... the suspension was filtered (130-110-916, Miltenyi Biotec), the cells were further resuspended in 5 volumes of cold HBSS and centrifuged at 300g for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with anti-O4 Microbeads (130-094-543, Miltenyi Biotec) and magnetic separation was done using Multi-24 Column Blocks and the MultiMACS Cell24 Separator Plus (130-095-692 and130-098-637 ...