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Citations for Becton, Dickinson and Company :
4201 - 4250 of 4284 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were washed three times with PBS and then incubated with primary antibodies anti-ASO (Ionis Pharmaceuticals. Carlsbad, CA) and anti-CD31 (BD Bioscience, San Jose, CA) with a 1:200 dilution in blocking buffer at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated as described above and stained at 4°C (15 min) with fluorochrome-conjugated antibodies against surface markers of endothelial cells (CD31 (BD Biosciences, Franklin Lakes, USA) and myeloid cells (CD45 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: qNSCs and aNSCs from the SVZ were FACS purified from GFAP::GFP mice as previously described (Codega et al., 2014) using the following antibodies: anti-mCD24 PE (1:2000; BD Pharmingen 12-0242-82), biotin conjugated anti-mCD133 (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with Alexa Fluor 647–conjugated anti– human CD24 and APC-H7 labeled anti–human CD44 antibodies (BD Pharmingen, San Jose, CA) at 4°C for 20 min ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Immunology 2020Quote: ... The following primary antibodies were used: AF-488 anti-rat CD3 (Biolegend. Clone 1F4, PE-Cy5 Mouse anti-rat CD4 (BD bioscience. Clone Ox-35), PE Mouse anti-rat CD8α (BD bioscience ...
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bioRxiv - Bioengineering 2021Quote: ... cells were stained with surface marker antibodies and analyzed with Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Propidium Iodide (5:1000 µL ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
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bioRxiv - Cancer Biology 2021Quote: ... Negative cells were sorted for the absence of PD-L1 by staining with a PE-labeled anti-mouse PD-L1 antibody (clone MIH5, BD Biosciences, Franklin Lakes, NJ) using a FACSAria III (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... harvested and stained for flow cytometry as described (37) using antibodies to human surface antigens: CD4-APCH7 (SK3, BD Biosciences Franklin Lakes, NJ, USA), CD8-PerCP (SK1 ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were FACS sorted four times for expression of CD40L using an anti-CD40L antibody (clone TRAP1, BD Bioscience, San Jose, California). This resulted in a stable CD40L-expressing cell line that was cultivated in G418 containing selection media to maintain expression ...
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Cancer Biology 2023Quote: Ba/F3 cells expressing human MPL protein that were either cultured normally or treated with drugs were harvested and stained for surface MPL using PE-conjugated anti-human MPL antibody (BD Pharmingen 562159,1:50 dilution) in staining buffer for 30 min at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... 1x106 cells in 100 μL were stained for 30 minutes at room temperature using the following antibodies: CD90-BUV395 (BD, #740260, 0.1 mg/μL), ECAD-AF405 (Novus Bio ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluoresceinated antibodies against (TCRý, CD4, CD8, CD3, CD3χ, CXCR5, PD1, and intracellularly for ZAP70 and IL-10) (BD Biosciences, San Jose, CA) and data acquired on a FORTESSA flow cytometer and analyzed using FlowJo Software (Ashland ...
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bioRxiv - Genetics 2023Quote: ... USA)/anti-rabbit IgG Alexa 488 (Jackson Immuno Research lab, West Grove, PA, USA) and 10µg/mL biotinylated anti-CD45 antibodies (BD Bioscience, Franklin Lakes, NJ, USA)/anti Rat IgG Alexa Plus 647 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... 1.5 µg/mL) [31] in the presence of anti-CD28 and anti-CD49d antibodies (both at 1 μg/ml, BD, Franklin Lakes, New Jersey) and brefeldin-A (10 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with primary antibodies for at least 1 hr at room temperature and secondary antibodies (for nonconjugated primary antibodies) for 30 min at room temperature with washes between the steps before analysis by flow cytometry (BD Fortessa or MACSQuant 16). To exclude dead cells ...
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bioRxiv - Cell Biology 2024Quote: ... upper membrane parts were first probed with rat monoclonal anti-pSMAD1/9 antibody (pS463/pS465, pS465/pS467, 1:1,000, #562508, BD Biosciences, East Rutherford, NJ, USA), or rabbit monoclonal anti-pSMAD2 (S465/467 ...
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bioRxiv - Developmental Biology 2024Quote: ... Animal caps were blocked for 1 hour using sheep serum and bovine serum albumin and then incubated in 1:250 anti-β-catenin antibody (BD Bioscience cat# 610154) in blocking solution overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The samples included for T cell phenotypic characterization were stained with a separate antibody cocktail containing T cell lineage markers (CD3-BV786 (BD, cat. #563799, clone SK7), CD4-BV650 (BD ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed with PBS+FBS and incubated with the monoclonal antibody anti-mouse CD8 T conjugated to Fluorescein Isothiocyanate (FITC) (BD-Pharmingen, San Diego, CA), which was suitable for determining the phenotype of CD8+ T cells ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked in 5% non-fat dry milk in TBS/Tw and then incubated overnight with primary antibody Syn-1 (BD Biosciences #610,787, 1:1000) or MJFR-14-6-4-2 (Abcam #209,538 ...
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bioRxiv - Microbiology 2023Quote: Surface markers of splenocytes were identified using monoclonal antibodies in conjunction with two immunofluorescence analysis with a flow cytometer (BD Accuri C6 Plus: BD, Mountain View, CA, USA). Antibodies used were Fluoroscein isothiocyanate (FITC)-conjugated anti-mouse CD4 and CD21/CD35 (CR2/CR1 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were blocked in 5% BSA and probed overnight at 4°C with the following primary antibodies and dilutions: p16 (1:200, #554079 BD Biosciences, San Jose, CA), p53 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in 100 μl of staining solution of FACS buffer containing biotinylated antibodies against CD31 (BD Biosciences, 558737, 1:100), CD45 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Genomics 2022Quote: The collected cells were first stained with live/dead aqua to exclude non-viable cells from the analysis and stained with fluorophore-conjugated antibodies against surface markers anti-CD45-BV786 (BD Bioscience Clone: OX-1), anti-CD11b-V450(BD Bioscience Clone ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4°C with primary antibodies: ALIX (BD Biosciences, 611620, 1:1000), calnexin (Santa Cruz ...
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bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
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bioRxiv - Immunology 2024Quote: ... Lymphocytes were cultured in RPMI supplemented with 10% FBS and 50 μM beta-mercaptoethanol (RPMI media) Lymphoblasts were generated by activation with plate bound murine anti-CD3ε antibody (BD Biosciences, Clone: 145-2C11) in the presence of 100 U/ml of human IL-2 and soluble anti-CD28 antibody (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed with phosphate buffer three times for 10 minutes each and incubated with primary antibody (rat anti-mouse CD31, BD Biosciences, 550274, 1:100) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... was prepared by coating the well with 50 μl of 10 μg/ml of anti-mouse IFNγ monoclonal capture antibody (BD Biosciences; cat. no. 551309) in PBS and allowed to incubate at 4°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... The ELISPOT plate was washed five times with PBST and 50 μl of 1 μg/ml of the anti-mouse IFNγ monoclonal detection antibody conjugated to biotin (BD Biosciences; cat. no. 551506) in PBS was added and allowed to incubate at room temperature for 2 hours ...
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bioRxiv - Immunology 2024Quote: ... according to the protocol from vendor and stained with intracellular cytokine specific antibodies Rat anti-mouse Rorγt- Brilliant Violet-650 (BD Biosciences, #564722, clone Q31-378), mouse anti-GATA3 Alexa Fluor F488 (BD Bioscience #560163 ...
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bioRxiv - Immunology 2024Quote: ... Sections were incubated overnight at 4°C with the following primary antibodies: anti- CD3e (T-cell marker, Hamster Anti-Mouse CD3e 500A2, catalog #553238, BD Biosciences, Franklin Lakes, NJ), anti-IgD (follicular B cell marker ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with staining buffer to remove excess antibodies and then fixed/permeabilized using mouse Foxp3 Buffer Set (BD Biosciences, San Jose, CA) according to manufacturer protocol ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight in 0.1M PBS + 0.1% TX-100 solution containing primary antibody for rat anti-CD31 (label endothelial cells; 1:200 dilution, BD Clone MEC 13:3: #553370) or sheep anti-CD93 (label endothelial tip cells ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin in PBS for 45 min at 37°C was followed by incubation with a primary antibody mix of anti-BrdU detecting IdU (mouse, #347580, BD, Franklin Lakes, NJ, USA) and anti-BrdU detecting CldU (rat ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Antibody staining was carried out for 1 hour at 4°C on rotation with the following antibodies: PAX6-Alexa Fluor 488 (BD Biosciences,1:20 dilution), EOMES-PE-Cy7 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM MgCl2) and allowed to settle for 1–2 min on anti-CD3ε antibody (30 μg/ml, BD Pharmingen, clone 145-2C11) coated coverslips ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked with 5% BSA in PBS and BrdU was then detected with a mouse anti-Brdu primary antibody (BD Biosciences, Clone B44, 347580) and goat anti-mouse AlexaFluor-488 conjugated secondary (1:500 ...