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Citations for Becton, Dickinson and Company :
4151 - 4200 of 4284 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the individual antibodies used for staining were mixed with one drop of negative control beads and anti-mouse IgG beads (BD Biosciences, CA, USA). For the dead cell stain ...
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bioRxiv - Immunology 2022Quote: ... cells were stained in FACS Buffer (1:200, PBS, 2% FBS, 2 mM EDTA, 0,05% NaN3) with antibodies recognizing: CD14 PECy7 (555399, BD Biosciences, Franklin Lakes, USA), CD16 BV510 (563830 ...
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bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88; and IL-5, BD Biosciences, #51-1806KZ) was added at a 1:250 dilution in ELISpot diluent (eBioscience ...
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bioRxiv - Microbiology 2022Quote: ... was used as primary antibody for samples and mouse anti-clathrin heavy chain clone 23 (1:2,000; BD Biosciences, Franklin Lakes, NJ, USA) for the detection of clathrin heavy chain as a loading control ...
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bioRxiv - Neuroscience 2022Quote: ... Following overnight incubation the plate was washed 3 times with DPBS and then incubated with 20 µl anti-CD45 antibody (BD Pharmingen, catalog #550539) in 12 ml of 0.2% BSA for a minimum of 2 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were dispersed into a single cell suspension using 0.05 % trypsin and subsequently incubated with an anti CD144 antibody (anti-VE-Cadherin; BD Biosciences, #562242, 1/50) for 30 min on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Samples are ultracentrifuged and pellets were resuspended and incubated onto 96 well ELISA plates containing 5ug/mL anti-syn42 monoclonal antibody (BD transduction #610-787). ELISAs were carried out on test samples run alongside serial diluted standard synthetic α-Syn ...
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bioRxiv - Immunology 2024Quote: ... The labeled cells were cultured in complete RPMI-1640 medium plus IL-2 (20 units/ml) or IL-2 and anti-CD3 antibody (1μg/ml) (BD Pharmingen (San Diego, CA), or alternatively plus IL-4 (4ng/ml ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Immunology 2023Quote: ... Surface antibodies were prepared at optimal dilutions (see Supplementary Table S1) in FACS supplemented with Brilliant Stain Plus buffer (BD; Franklin Lakes, NJ), then were added to samples in blocking solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with multiple flow cytometry validated antibody cocktails (see below) and analyzed using either BD Fortessa or LSRII cytometers (BD Horizon, BD, Franklin Lakes, NJ). Data were analyzed and quantified using FlowJo v10.6 software (RRID:SCR_008520) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5-μm sections were stained with the following antibodies following citrate antigen retrieval and blocking with 10% normal donkey serum: mouse anti-glutamine synthetase (BD Bioscience, 1:2000), mouse anti-Ki67 (BD ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... pre-incubated with anti-mouse CD16/CD32 (FcRII block) and then stained with the following anti-mouse monoclonal antibodies: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Developmental Biology 2023Quote: ... and then blocked in 1% bovine serum albumin in PBST before addition of primary antibodies against E-cadherin (1:200, BD Transduction Laboratories, 610181), laminin (1:200 ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 4°C for 30 min with a B cell surface marker antibody cocktail (Table S11) with 10 µl Brilliant Stain Buffer Plus (BD, cat. No. 566385) diluted in PBS containing 1% BSA up to a volume of 100 µl ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with flow cytometry antibodies (Supplemental Table 9) in flow cytometry staining buffer (PBS, 1% BSA) and brilliant staining buffer (563794, BD, Franklin Lakes, NJ). Cells were analyzed using a 5-laser (16UV-16V-14B-10YG-8R ...
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bioRxiv - Immunology 2023Quote: ... pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL, clone: 145-2C11, BD Biosciences, San Diego, CA, USA) and supplemented with soluble anti-CD28 antibodies (2ug/mL clone ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... we stained cells expressing wild-type and mutant SFV glycoproteins with a mouse anti-SFV immune ascitic fluid (ATCC Cat#: VR-1247AF) or a control mouse IgG2b antibody (BD Biosciences Cat#: 557351). Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat# ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% skim-milk diluted in 1X TBST and incubated with primary antibodies overnight [RB (BD Pharmingen #554136, 1:500), p53 (Pierce #MA5-12453 ...
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bioRxiv - Neuroscience 2024Quote: ... The cover slips were then permeabilized with 0.3% Triton X-100 and sequentially stained with rat anti-CD44 antibody (BD Biosciences, 550538; 1:200), and corresponding Alexa 488-conjugated secondary antibodies (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The antibodies used for stimulation were anti-human CD3 (clone UCHT1, plate bound) and anti-human CD28 (clone 28.2, BD Biosciences, San Jose, CA) at 1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed with FACS buffer stained with antibodies to detect surface expression of CD4 (GK1.5) and CD25 (PC61) (BD Biosciences, Franklin Lakes, NJ), whereas intracellular expression of FoxP3+ was stained using an anti-Mouse/Rat FoxP3 (FJK-16s ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and incubated in following antibodies for 30 minutes at 4°C in the dark: PerCP rat anti-mouse CD45 (557235, BD Biosciences, 1:50), FITC rat anti-CD11b (553310 ...
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bioRxiv - Immunology 2024Quote: ... Cell suspensions were then incubated for 30 min with anti-mouse antibodies (1:500 dilution) against CD45 Brilliant Violet 711 (0.2 mg/ml; Clone 30-F11, 563709, BD Biosciences, San Jose, CA), CD45 PE-Firetm700 (0.2 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with antibodies to cytokines (Table S2) and samples acquired on a flow cytometer (MACSQuant VYB, BD Fortessa or Cytek Aurora). Data was analysed in FlowJo.
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bioRxiv - Physiology 2024Quote: ... then cells were washed with 1x PBS and resuspended in FACS buffer and non-specific binding to Fc receptors was blocked by incubation with anti-CD16/32 antibody (BD Pharmingen, Cat# 553141) and the FACS antibodies BV711 conjugated anti-CD11b and Pacific blue conjugated anti-CD45 antibodies (Cat# 101241 ...
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bioRxiv - Microbiology 2024Quote: ... The homogenized cell suspension was washed and resuspended in FACS buffer containing the PE-conjugated Rat anti-mouse F4/80 antibody (BD Horizon, Cat-565411) or eFluor450-conjugated anti-mouse CD11c antibody (eBioscience) ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell samples were stained with the following antibodies in cell staining buffer for 40 min at 4 °C: anti-mouse-CD3e-FITC (553061, BD Biosciences, 1:200), anti-mouse-CD4-PercpCy5.5 (550954 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were dissociated and stained with an A647- conjugated antibody against the target gene product CD9 (#341648, BD Biosciences, Franklin Lakes, NJ, USA). Cells were suspended in PBS and FACS sorting was done using a BD Influx (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... cells were stained in a mixture of propidium iodide and anti-human antibodies anti-IgG (BV605; Clone G18–145; BD Biosciences Cat # 563246), anti-IgA (FITC ...
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bioRxiv - Cancer Biology 2024Quote: ... blood from non-tumor bearing mice) were positively enriched for eosinophils using a PE anti-mouse Siglec-F antibody (562068, BD Biosciences; E50-2440) and anti-PE microbeads (130-042-401 ...
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bioRxiv - Cell Biology 2024Quote: ... A master mix (5 µL of each fluorochrome-conjugated antibody + 10 µL of Brilliant Staining Buffer (Figure 1 a & b) (BD Horizon™ #659611) or Super Bright Complete Staining Buffer ...
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bioRxiv - Genetics 2024Quote: ... Cells were stained with 5 ml PE Mouse Anti-Cardiac Troponin T antibody (564767, clone 13–11, BD Biosciences, San Jose, CA, USA) for 45 min at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were incubated with PBS/1% BSA for 30 min at 4 °C for blocking unspecific binding and washed with PBS prior to the incubation with PE-conjugated anti-human CD69 antibody (cat. 555531, BD Biosciences, BD PharMingen) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Antibody-stained cells were washed in FACS staining buffer and resuspended in 20 µL 7-actinomycin D (7-AAD, BD Pharmigen flow kit) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: EMA-labeled DNA was added to MM cells and incubated overnight followed by staining with anti-human CD138-FITC antibody (BD, cat no. 552723). Presence of DNA was determined using an ImageStreamX Mark II instrument (Amnis Corporation ...
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bioRxiv - Molecular Biology 2021Quote: ... stained with a collection of different fluorescent labelled antibodies (CD4-BV785; BioLegend, San Diego, CA, USA; CD25-PE-Cy5.5; BD Bioscience; and CD54-AF700; EXBIO) and analysed on a Cytoflex flow cytometer (Software CytExpert 2.3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Enumeration of human CD45+ cells in spleen and bone marrow was performed by flow cytometry after staining with BV605-conjugated human-specific CD45 antibody (clone HI30, BD Biosciences, San Jose, CA) using a BD LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... The DCs were phenotyped using phycoerythrin-conjugated (PE) monoclonal antibodies (mAbs) against CD83 and CD14 setting the cutoff for positive cells with the isotype control IgG2a (BD Pharmingen, Franklin Lakes, NJ). Immature DCs were transferred to new plates at a concentration of 4×105cells/mL ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... Synaptic ribbons were labeled with anti-CtBP2 (1/200 Goat polyclonal, Santa Cruz, USA ; cat # SC-5966 or 1/200 Mouse IgG1 monoclonal antibody BD Biosciences, cat # 612044). Otoferlin was labeled with a mouse monoclonal antibody (1/200 AbCam ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 4% milk for 1 hour and incubated overnight at 4°C with shaking in the appropriate primary antibody against Nischarin (1:500, BD Biosciences, Cat No: 558262), GFP (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...