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Citations for Becton, Dickinson and Company :
3751 - 3800 of 4284 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Blood samples from the same animals were also stained with the same antibody panel before red blood cell lysis using BD Pharm Lyse lysis buffer (BD Biosciences). All results were acquired using a BD LSRFortessa (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: Spleen and liver cells were incubated (20 min, 4°C) with anti-Fc-gamma blocking antibody (clone 2.4G2) (BD Biosciences, Belgium) and were subsequently stained with fluorescent conjugated antibodies (30 min ...
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bioRxiv - Immunology 2020Quote: ... naïve splenic B cells were enriched by the depletion of CD43 expressing cell using biotinylated anti-CD43 antibody (Clone S7, BD Biosciences), BD IMag Streptavidin Particles Plus and the IMag® system (BD Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated during 30 minutes at 4°C with the following primary antibodies: CD11b (clone M1/70, AF647, #557686, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated with the following primary antibodies during 30 minutes at 4°C: CD11b (clone M1/70, APC-Cy7, #557657, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein (51)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... MLN cells and lung CD11c+ cells were stained for flow cytometry analysis as described above using following fluorophore-conjugated antibodies DAPI (BD Biosciences), CD45 (30-F11 ...
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1 perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2022Quote: ... The membranes were then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk for 2 h ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk in each channel for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) was performed on FFEP blocks using mouse monoclonal antibodies SMARCB1/INI1 (Clone 25/BAF47, dilution 1:200, BD Bioscience) and CD70 (Clone 301731 ...
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bioRxiv - Cell Biology 2022Quote: ... cell sorting was employed by labeling intact cells with the fluorescently conjugated HER2 antibody ErbB2 PE-Vio770 and then subjected to fluorescence-activated cell sorting using a FACS Aria III (BD Biosciences), selecting the cells in the top 10% of HER2 expression to generate MDA-MB-231-HER2 stable cells.
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... and the cells were labeled with antibodies as also described above and were then analyzed using a BD FACSCanto cytometer running BD FACSDiVa software (BD Biosciences), using a violet (405 nm ...
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bioRxiv - Genomics 2022Quote: ... This was followed by the secondary labeling step with 1:200 dilution of Streptavidin PE-Cy7 antibody (BD Biosciences, Cat#: 557598). Cells were isolated and sorted using an Influx Cell Sorter Instrument (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained with fluorochrome-conjugated antibodies or influenza HA-specific (HA518–526) H-2Kd tetramer-IYSTVASSL (MBL) reagent and analyzed using a flow cytometer (BD Biosciences). BMDCs (105 cells ...
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bioRxiv - Immunology 2020Quote: ... and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences), phorbol 12-myristate 13-acetate (PMA)/ionomycin or autoantigens (1 μg/ml of DNA or SM (Immunovision)) ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with fluorescent-conjugated anti-mouse antibodies after blocking CD16/CD32 with Fc Block (BD Biosciences, San Jose, CA). For intracellular staining ...
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bioRxiv - Immunology 2020Quote: Colonic LP samples (2 million cell/staining) were stained at 4°C for 20 min with the following antibodies: Ly6C-FITC (AL-21, BD Pharmingen), F4/80-PE (BM8 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C and secondary antibodies for 30 min in RT in Perm/Wash buffer (554714, BD Biosciences) containing 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... 5×106 cells were stained for 30 min at 4°C with the fluorescent probes, a viability marker (LiveDead-eF780, eBiosciences) and the following B cell-specific antibodies: anti-CD20-PE-CF594 (2H7; BD Biosciences), anti-IgG-PE-Cy7 (G18-145;BD Biosciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... which were then stained with the above-mentioned antibodies and LT-HSCs were sorted using a BD FACS Aria III (BD Biosciences).
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were incubated overnight with different anti-aSyn primary antibodies with different binding properties: either 1:1000 mouse anti-aSyn (BD Biosciences), 1:3000 mouse anti-aggregated-aSyn (clone 5G4 ...
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bioRxiv - Bioengineering 2021Quote: ... We incubated the ECs in a 1:500 primary antibody solution containing Mouse anti-CD144 (VE-Cad, BD Pharmingen Cat. #55561) in ADB overnight at 4 oC ...
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bioRxiv - Immunology 2020Quote: ... mouse anti human ICAM-1 (CD54) and mouse anti human VCAM-1 (CD106) antibodies and fixed with FACS lysing solution (BD Bioscience). Surface levels were determined by flow cytometry (Gallios ...
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bioRxiv - Cancer Biology 2021Quote: ... and cells incubated for 30 min on ice with following antibodies: PE-conjugated anti-CD11b (M1/70, 1:3000, BD Biosciences), APC-conjugated anti-F4/80 (for macrophages ...
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bioRxiv - Immunology 2021Quote: ... for 10 min followed by staining with antibodies: 1 μl of PE-conjugated anti-mouse Ly-6G (BD Pharmingen, CA, USA), 1 μl of FITC-conjugated anti-mouse CD206 (Biolegend ...
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bioRxiv - Immunology 2020Quote: We centrifuged the samples and resuspended each in 1 mL of an antibody master mix consisting of Brilliant Buffer (BD Biosciences) and anti-CCR6 - PECy7 (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... The purity of the cells was confirmed by staining cells with PE-CD71 and allophycocyanin (APC)-Ter119 antibodies (all from BD Biosciences) by flow cytometry ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... activation of T-cells was measured by flow cytometry using the anti-human CD4 (FITC)/CD69 (PE)/CD3 (PerCP) antibody staining solution (BD Biosciences) in combination with anti CD25 (APC ...
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bioRxiv - Cancer Biology 2021Quote: ... 1x105 human EGFR-mutated NSCLC cells plated in 60-mm dishes were stained with fluorescein isothiocyanate (FITC)-conjugated anti-Annexin V antibodies (BD Biosciences) together with propidium iodide (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... Kidney sections were blocked (10% normal goat serum and 1% bovine serum albumin (BSA) in PBS) and incubated overnight at 4°C with primary antibody ((Ly6G, 1:10; BD/Pharmingen), FITC-C3 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with PE-conjugated mouse anti-Human CD44 and mouse anti-Human CD184 antibodies (BD Biosciences, San Jose, CA) at a concentration of 5μL/10 million cells ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with the medium and stained with subsequent antibodies/reagents using the Brilliant Stain Buffer Plus (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cell were washed three times with HF buffer and incubated with secondary antibodies APC-Cy7 goat anti-rabbit IgG (BD, 611203) used at 1:200 dilution and AF647 Donkey anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Cells were then incubated with 5ul human Fc Block with 95ul 2% FBS in PBS at 4°C for 15 mins before staining with SCMK and AbSeq antibodies (BD Biosciences) at 4°C for 60 min ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences, 1:100), -Sca-1 (E13-161.7 ...
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bioRxiv - Immunology 2021Quote: ... The stained cells were subsequently washed with FACS buffer to remove unbound antibodies and probe and then filtered through 70-μm cell mesh (BD Biosciences) before processed in the FACS sorter ...
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bioRxiv - Immunology 2021Quote: ... cryo-preserved PBMCs were first thawed at 37°C in a water bath and washed with an RPMI medium containing 10% fetal bovine sera (FBS) following incubation with fluorescently-labeled antibodies (BD Biosciences) against cell surface markers for CD3 (PE-Cy7) ...
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bioRxiv - Developmental Biology 2021Quote: Mouse femur single cells were obtained as described above in the flow cytometry section and stained with biotin-conjugated antibodies for Lin (Ter119, Gr-1, B220, CD3e) (BD, 559971) followed by negative selection using streptavidin-coated magnet beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... bone marrow cells were subjected to red blood cell lysis and stained with fluorochrome-conjugated anti-mouse antibodies to CD11b (Mac-1, BD Biosciences) and/or Ly6G (1A8 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Synapsin (1:500) and anti-CD133 (1:500) were used after Fc blocking with CD16/32 antibody (553141; BD Pharmingen). Respective Secondary antibodies ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated with detection biotinylated anti-IFNγ antibody for 2h and followed by incubation with streptavidin-horse radish peroxidase (BD Biosciences) for 1h ...
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Immunology 2020Quote: ... The cells (2-4 × 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibodies to either IFN-γ or IL-4 (BD), and were exposed to various stimuli (e.g ...
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bioRxiv - Immunology 2020Quote: ... then binding was detected with specific alkaline phosphatase-conjugated secondary antibodies (anti-mouse total IgG and anti-monkey total IgG from Sigma and anti-mouse IgG1 and IgG2a from BD Biosciences). Alkaline phosphatase substrate was prepared by dissolving SigmaFast (Sigma ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained for surface markers using combinations of the following directly conjugated monoclonal antibodies: anti-CCR7–BV650 (clone 3D12; BD Biosciences), anti-CCR7–PE-Cy7 (clone 3D12 ...
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bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.
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bioRxiv - Immunology 2021Quote: ... and cell surface markers were stained with the following anti-mouse antibodies at 1:200 dilution per 106 cells: Ly6C (AL-21; BD Biosciences), Ly6G (1A8 ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions from mesenteric lymph nodes IL-4 ELISPOT were performed using mouse IL-4 ELISPOT pair antibodies (BD Bioscience) 200,000 cells/well were cultured on multiScreen IP filter plate (Millipore ...