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Citations for Becton, Dickinson and Company :
3801 - 3850 of 4284 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Sections were stained using the following antibodies: rat anti-mouse Ly-6C/Ly-6G (RB6-8C5, BD Bioscience, #550291, 1/200) followed by AlexaFluor647-conjugated goat anti-rat IgG (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were stained with fluorophore-conjugated antibodies and analyzed on a MACSQuant flow cytometer (Miltenyi Biotech) or sorted using the FACSAria Fusion cell sorter (BD Biosciences). Flow cytometry analysis was based on viable and single-cell gaiting strategies ...
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bioRxiv - Immunology 2020Quote: ... Single-cell suspensions were first stained for viability with LiveDead eFlour 780 stain (eBioscience) and stained with antibodies diluted in Brilliant Stain Buffer (BD Biosciences) or FACS buffer (1% BSA in PBS) ...
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bioRxiv - Immunology 2020Quote: ... for the last 4hrs before subjecting for fluorochrome-conjugated antibody staining for surface markers and intracellular cytokine (Perm/Fix buffer, BD Biosciences). Cells were analyzed by 24-color compatible full-spectrum Cytek Aurora flow analyzer (College of Veterinary Medicine ...
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bioRxiv - Immunology 2021Quote: ... or with no target cells (or an E:T of 1:0) and cultured overnight before being staining with allophycocyanin (APC)-conjugated anti human-CD69 antibody (BD Biosciences #555533). Flow cytometry was performed using a BD- Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... a combination of the following anti-mouse antibodies was used to stain for surface markers (all from BioLegend, eBioscience, or BD Biosciences): anti-CD3ε (145-2C11) ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block and subsequently stained with a combination of the following anti-mouse antibodies (all from BioLegend, eBioscience, or BD Biosciences): anti-CD8α (53-6.7) ...
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bioRxiv - Immunology 2020Quote: ... cells were first stained for viability with LiveDead eFlour 780 stain (eBioscience) for 30 minutes and then stained with antibodies and MHC I tetramers diluted in Brilliant Stain Buffer (BD Biosciences) for 60 minutes ...
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bioRxiv - Immunology 2020Quote: ... and BM as previously described (Ciucci et al., 2015) and were stained with different antibodies used for FACS (FACS canto, BD Biosciences) are indicated in the figures.
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bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescent labelled anti-mouse antibodies after blocking CD16/CD32 with Fc Block (BD Biosciences, San Jose, CA). Flow cytometry analysis was performed using following antibodies ...
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bioRxiv - Immunology 2020Quote: Single-cell suspensions from the intestine or bone marrow were stained with fluorescently labelled antibodies and sorted (purity > 98%) using a BD FACSAria III cell sorter (BD Biosciences). For ILC ...
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2020Quote: ... Intracellular cytokine staining of cells were stained with following antibodies with maintaining supplier’s instructions: V500 anti-mouse CD45 (BD Bioscience, USA), FITC anti-mouse CD4 (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Purified MDSCs from spleen and bone marrow cells were isolated using a mojosort magnet followed by cell surface staining with CD11b and Gr1 monoclonal antibodies for cell sorting on BD FACS Aria III cell sorter (BD Biosciences). Similarly ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were obtained from the spleen of naive mice following antibody labeling and sorted on flow cytometer BD FACS Aria III cell sorter (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... IFNγ-producing cells were stained with IFNγ Detection Antibody in conjunction with cell surface markers (listed below) and analyzed on an LSRFortessa flow cytometer (BD Biosciences). Antibodies used included CD3e (145-2C11) ...
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Immunology 2021Quote: ... as a negative control in the presence of αCD28/αCD49d co-Stim antibodies (1 μg ml−1) GolgiStop (containing Monensin, 2 μmol/L), GolgiPlug (containing brefeldin A, 10 μg ml−1) (BD Biosciences) and anti-CD107α BV421 antibody (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibodies for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before analysis using BD Symphony Flow Cytometer ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with cell phenotyping antibodies in a 1:1 volume ratio of 3% FBS and Brilliant Stain Buffer (BD Biosciences) according to standard procedures ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: Immune cells for bulk mRNA sequencing were incubated with Fc Block for 20 minutes and then stained with one of six panels of directly conjugated antibodies for 30 minutes: anti-human CD16 (BD 558122), CD123 (BD 560826) ...
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bioRxiv - Immunology 2021Quote: ... Parasites were then washed with FACS buffer and placed on ice for incubation with anti-isotype detection antibodies depending on application: anti-IgG3-BV421 (R40-82, BD Bioscience), anti-IgM-PE/Cy7 (RMM-1 ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated and immunolabeled with anti-CD4 and anti-Foxp3 antibodies and BrdU incorporation was analyzed by a BrdU Flow kit per the manufacturer’s protocol (BD Pharmingen, 552598) (15).
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bioRxiv - Immunology 2021Quote: ... Cells were then washed in 1x eBioscience Permeabilization Buffer twice and incubated with indicated intracellular antibodies including anti-T-bet-BV785 (4B10, BD Biosciences), anti-EOMES-PerCP-ef710 (X4-83 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with a cocktail of antibodies (P1, P2, P3 and P4) in Cell Staining Buffer plus BD Horizon Brilliant Stain Buffer Plus (BD, 566385) (Key Resources Table ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognises residues 91-99 from the NAC region of human α-synuclein (20)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 7 days and immunostained for flow cytometry (Antibodies: CD34, clone 581; CD117, clone: 104D2; CD11b, clone ICRF44 (BD Pharmingen) and 1:50 FcR Blocking Reagent (Miltenyi Biotec)) ...
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bioRxiv - Microbiology 2023Quote: ... Differentiation was confirmed by measuring expression of CD11b and CD14 markers by flow cytometry (antibodies: CD11b, clone D12; CD14, clone M5E2, BD Pharmingen).
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1× perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used on cells isolated from bleeds and spleen/bone marrow of NSG treated mice: CD3 APC R700 (BD 565119), CD4 BB700 (BD 566392) ...
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bioRxiv - Immunology 2023Quote: ... Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution, clone R2-40, BD Bioscience) for 30 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... Human erythrocyte engraftment was assayed by flow cytometry of peripheral blood incubated with APC-conjugated TER-119 antimouse monoclonal antibody (BD Biosciences), with gates at APC+ and APC- (mouse and human erythrocytes ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs were stained with a panel of fluorochrome-conjugated antibodies reactive with the following cell surface markers: CD45-APC/Cy7 (clone 30-F11; BD Biosciences), CD3-PE/Cy7 (clone 500A2 ...
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bioRxiv - Immunology 2023Quote: ... with the same panel of human antibodies as used for cell sorting and analyzed by flow cytometry on a FACS Fortessa (BD Biosciences). UltraComp eBeads (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by two PBS-wash steps and secondary antibody staining and analysis on an LSRFortessa X-20 flow cytometer (BD Biosciences), which was also used for all downstream flow cytometry.
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bioRxiv - Cancer Biology 2023Quote: ... tumor or normal organ tissue slides were incubated with primary antibody of Ki-67 (1:10000 dilution; Cat# 550609, BD Biosciences), p-Abi-1-S323 (1:500 dilution ...
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bioRxiv - Pathology 2023Quote: ... then incubated for 20 minutes at 4°C with a combination of the following mouse anti-human antibodies: AF488 anti-CD3 (clone UCHT1, BD Biosciences), PE anti-CD203c (clone NP4D6 ...
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bioRxiv - Bioengineering 2023Quote: The following primary antibodies were used as previously demonstrated in MDCK cells: purified mouse anti-p120 catenin 56 (BD Biosciences, 610133) at a dilution of 1:200 and recombinant rabbit monoclonal anti-vinculin 35 (abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated with an intracellular antibody panel for 30 min at RT in 1x permeability/wash buffer (BD #554723), including NeuN-APC (1:50) ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was discarded and the pellet was resuspended in Staining buffer containing the Human BD Fc Block™ antibody (BD, 564220) and incubated with agitation for 15 min at 4°C in a cold room ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for 20 min a 4°C with the appropriate combinations of fluorochrome-conjugated anti-human antibodies for the identification of CD8 T cells and sub-populations: CD3 BV480 (clone UCHT1, BD Biosciences), CD8 BV421 (clone RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... purified NK cells were incubated for 10 minutes at room temperature with 2.5µg of Human BD Fc Block followed by addition of 10μg/mL of anti-NKG2D purified antibody (BD Biosciences, clone 1D11) or isotype control antibodies prior to addition of SVG-A cells ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently hLSECs were purified via FACS by labelling with mouse anti-human CD31-FITC conjugated antibody (clone WM-59, BD Biosciences). Two independent preparations of transplanted iEC were used for single cell RNAseq ...
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bioRxiv - Bioengineering 2023Quote: ... At the end of the co-culture the cells were washed twice and incubated with human or mouse Fc receptor blocking antibodies (BD Biosciences). Human macrophages were stained with anti-CD11b (M1/70 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then washed and stained using anti-mouse monoclonal antibodies: CD45.1 Brilliant Violet 510 (clone A20, BD Pharmingen, RRID: AB_2739150), CD45.2 FITC (clone 104 ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...