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Citations for Becton, Dickinson and Company :
3901 - 3950 of 4284 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the plate was washed 5x with PBST 0.05% and incubated with biotinylated antibody G1 (Serotec MCA336B) or G2A (BD bioscience 553388) for 60min at RT ...
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bioRxiv - Immunology 2023Quote: ... and anti-MCL-1 A647 (1:1000, clone 19C4-15, WEHI antibody facility) and analysed on a Fortessa X20 (BD Biosciences) or Cytek Aurora spectral flow cytometer (Cytek Biosciences) ...
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bioRxiv - Physiology 2024Quote: ... endothelial cells were purified by two consecutive immuno-selection procedures using magnetic beads conjugated with anti-ICAM-2 antibody (BD Pharmingen) and plated into 75 cm2 tissue culture coated flasks ...
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bioRxiv - Bioengineering 2024Quote: ... Viability dye was quenched by washing with FACS buffer (PBS + 2% FBS + 1 mM EDTA) before addition of surface staining antibodies in a 1:1 dilution of Brilliant Stain Buffer (BD 563794) in FACS buffer ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed with fixation buffer and then permeabilized with Cytofix / Cytoperm (e-bioscience) followed by staining for 60 minutes with antibodies that were diluted in 1X perm buffer (BD, 554723). Flow cytometry data acquisition was performed either on LSR Fortessa X-20 (BD ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cell Biology 2022Quote: For 3T3 NIH fibroblasts cells infected with C/EBPα-ER-IRES-hCD4 (WT and T35A) were incubated with 0.1µg per 1 million cells of both Fc block and hCD4-Biotin antibody (BD Pharmingen, Cat#555347) for 10 and 20 minutes respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... and engraftment of human cells in the bone marrow and spleen was measured with flow cytometry (CD45-PE-Cy7 antibody, BD Biosciences). Single-cell suspensions were prepared from the bone marrow ...
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bioRxiv - Cell Biology 2022Quote: ... Phenotypic analysis was performed with the following antibodies: Rat anti-mouse active β1-integrin (active CD29 Clone 9EG7, 553715; BD Biosciences); Alexa647-Armenian hamster anti-mouse β1-integrin (total β1-integrin 102214 ...
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bioRxiv - Cell Biology 2022Quote: ... The intracellular staining was carried out according to the manufacturer’s instructions using Fixation/Permeabilization kit (eBioscience) in the presence or absence of antibodies against OCT3/4 (OCT4) (AlexaFluor 647 mouse anti-OCT3/4, BD Biosciences), SOX17 (APC Goat anti-SOX17 ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with FITC-conjugated anti-BrdU antibody and then analyzed by flow cytometry with a BD FACSCanto II system (BD Biosciences). Data are shown as mean ± standard deviation and are representative of three or four independent experiments ...
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bioRxiv - Immunology 2022Quote: ... stained on ice for 30’ with relevant cocktails of antibodies and populations of interest were sorted at 4°C on an BD FACSARIA (BD Bioscience) using a 70 μM nozzle in 50% FBS RPMI ...
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bioRxiv - Immunology 2022Quote: ... intracellular cytokine staining was performed at 4°C for 30 minutes using a cocktail of antibodies resuspended in 1x Perm/Wash buffer (BD Biosciences); intracellular antibodies included IFNγ APC-Cy7 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Extracellular staining was performed by incubating the cells for 30 minutes on ice in the dark with the following antibodies: Anti-CD19 (clone SJ25-C1, BD Bioscience), anti-CD27 (clone L128 ...
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bioRxiv - Immunology 2022Quote: The hybridoma cell lines secreting SOSV-reactive antibodies were cloned using single-cell sorting on a BD FACSAria III cytometer (BD Biosciences) or SH800 Cell Sorter (Sony Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... ELISAs were performed using paired rat anti-mouse IgE antibodies (capture with 1 μg/mL purified R35-72, BD Biosciences, detect with biotinylated R35-118, BD Biosciences) and streptavidin-HRP (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: To analyze the major thymic stromal cell types in human samples single cells suspensions non-specific antibody binding was blocked with human Fc-Block (BD Bioscience) and the cells were subsequently stained with CD45-PE ...
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bioRxiv - Immunology 2022Quote: ... Compensation controls were obtained by incubating each single antibody with the anti-mouse Ig,k/Negative Control Compensation Particle Set (CompBeads, BD Biosciences). Fluorescence Minus One controls (FMO ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody bound to RBD in the presence of competitor was stained with Streptavidin-PE at a 1:1000 dilution (BD Biosciences) and analysed on a 200 Bioplex instruments ...
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bioRxiv - Immunology 2022Quote: Naive and effector/memory CD4+ T cells were isolated by Fluorescence-activated Cell Sorting (FACS) upon staining with anti-CD4 antibody (BD 562970), anti-CCR7 antibody (353227 BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Immunology 2022Quote: RFP+GFP+ FOXP3+ Tregs and RFP+GFP- exFOXP3 T cells isolated from spleen and CNS from EAE mice were stained with sample tag antibodies (Ms Single Cell Sampling Multiplexing Kit, BD Biosciences). Next ...
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bioRxiv - Bioengineering 2023Quote: ... TCR expressing cells were mixed with K562-A2 cells at a ratio of 1:2 (Effector:Target) in the RPMI media and supplemented with 1 µg/ml of anti-CD28/CD49d antibodies (BD Biosciences, 347690) and 1 µg/ml of cognate peptides ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were stimulated with the indicated agonists and labelled with a PS detecting antibody (CyTM 5 Annexin V, #559934, BD Biosciences) for 15 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
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bioRxiv - Developmental Biology 2023Quote: ... and then resuspended in 100 μL Annexin V binding buffer with either (i) FITC-Annexin V conjugated antibody (1:33 dilution in TBST) and BD propidium iodide staining solution (BD Pharmingen) or (ii ...
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bioRxiv - Immunology 2023Quote: ... BM aspirate samples from heathy donors using a Ficoll density gradient and stained with the following anti-human antibodies: IgD-Brilliant Violet 480 (Cat. No. 566138; BD Biosciences), CD3-BUV737 (Cat ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents: PE-conjugated rat anti-mouse Siglec-F (E50-2440; BD Biosciences), PE-TxR or PE-Cy5-conjugated rat anti-mouse CD11b (M1/70.15 ...
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bioRxiv - Cancer Biology 2023Quote: ... mice bone marrow mononuclear cells were first stained with lineage (Lin) cocktail containing biotinylated antibodies against Ly-6G/Ly-6C (Gr-1) Biotin RB6-8C5 (BD Bioscience) (1:100) ...
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bioRxiv - Cancer Biology 2023Quote: ... coverslips were incubated with primary antibodies (mouse anti-HA 1:200, Cell Signaling Technology, 2367; mouse anti-CtBP2, 1:250; BD Biosciences Cat# 612044 ...
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bioRxiv - Physiology 2024Quote: Isolated ileal and colonic lamina propria cells were blocked using anti-CD16/32 antibody (clone 2.4G2; BD Biosciences, Mississauga, ON, Canada) for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... and subsequentenly with primary antibodies (1/30 Ki-67 Cat. 550609 and 1/200 Anti BrdU Cat. 555627, both in 1% BSA, BD Pharmingen) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with a mouse pan α-synuclein primary antibody (Syn1, BD Biosciences, NJ, USA; #610786) diluted 1:5,000 in blocking solution ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... and stained for 30 min at 4 °C with the following antibody mixture: PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... CD34+ cells were labeled with APC or APC-Cy7 conjugated anti-CD34 antibodies (clone 561; BD Pharmingen, San Jose, CA, USA) at a concentration of 1:20 dilution and anti-APC MicroBeads at a concentration of 1ul/1×107 cells ...
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bioRxiv - Microbiology 2024Quote: ... washed with PBS-T and kept overnight in 1% BSA solution in PBS-T containing a 1:500 dilution of anti-rabbit-Vi-polysaccharide antibody (BD BioSciences) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... cultured pMacs in 24-well plates were incubated in primary anti-MHC-II monoclonal antibody (mAB; BD Biosciences, see Table 5) at a final concentration of 5μg/mL for 20 minutes at 4°C in FACS buffer containing 10% normal goat serum ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated on ice for 5 min with rat anti-CD16/CD32 antibody (BD Biosciences, clone 2.4G2, 5 µg/ml) and Fixable Viability Dye eFluor 660 or UV455 (eBioscience ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cultured on anti-hamster IgG- coated plates in the presence of hamster anti-CD3 epsilon and anti-CD28 antibodies (BD Biosciences), neutralizing anti-IL-4 antibody (eBioscience) ...
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bioRxiv - Immunology 2024Quote: ... cultured pMacs in 24-well plates were incubated in primary anti-MHC-II monoclonal antibody (mAB; BD Biosciences, see Table 5) at a final concentration of 5μg/mL for 1 hour at 37°C in FACS buffer to allow for the antibody-binding and subsequent uptake of MHC-II complexes ...
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bioRxiv - Immunology 2024Quote: ... 1 million cells per condition were incubated with the following antibodies: CD11c PE Cy7 (BD, CAT# 558079, clone HL3, 1:500), SiglecF PE (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... was determined using flow cytometry after incubation with a PE-conjugated anti-human CD25 antibody (cat. 555432, BD Biosciences, BD PharMingen).
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bioRxiv - Bioengineering 2024Quote: ... was determined using flow cytometry after incubation with a PE-conjugated anti-human CD25 antibody (cat. 555432, BD Biosciences, BD PharMingen).
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bioRxiv - Bioengineering 2024Quote: ... the cells were incubated with PBS/1% BSA for 30 min at 4 °C for blocking unspecific binding and washed with PBS prior to the incubation with PE-conjugated anti-human CD69 antibody (cat. 555531, BD Biosciences, BD PharMingen) for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... with fluorescently-conjugated antibodies specific for mouse CD45 (PE-Cy7 30-F11; Thermo Fisher Scientific or BUV395 30-F11; BD Biosciences) and human CD45 (V500 HI30) ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed with the kit washing buffer and stained with the intracellular mix containing the following anti-human antibodies: Ki67 (BUV395, 1/160, BD Biosciences) and Foxp3 (AF488 ...
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bioRxiv - Immunology 2024Quote: ... with regular verification of the depletion step by staining the incubated cells with an anti-human IgG fluorescent antibody and running these cells on the LSRII flow cytometer (BD Biosciences) (Figure 1A) ...
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bioRxiv - Immunology 2024Quote: ... and then stained with the following antibodies to validate B cell depletion: CD45 BUV395 (BD Biosciences clone 30-F11; 1:200), CD19 BV737 (BD clone 1D3 ...