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Citations for Becton, Dickinson and Company :
2151 - 2200 of 2202 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The strain was revived from a 20 % glycerol conidial suspension stored at -80°C and then maintained on potato dextrose agar media (PDA; BD Difco Laboratories, France) at 20°C in dark conditions ...
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bioRxiv - Immunology 2024Quote: ... Mtb Erdman smyc’::mCherry and Mtb Erdman hsp60::GFP have been described previously.80,81 The Mtb Erdman-Lux strain was generated by transformation with the pMV306G13+Lux plasmid.82 Mtb was grown to log phase at 37°C in MiddleBrook 7H9 broth supplemented with 10% oleic acid/albumin/dextrose/catalase (OADC Enrichment; Becton, Dickinson and Company), 0.2% glycerol ...
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bioRxiv - Biophysics 2021Quote: ... DC were fixed with 4% paraformaldehyde and kept at 4°C until acquisition using a LSRII analytical flow cytometer (BD Biosciences, San Jose, USA). FlowJo software (v10 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 37°C and dead cells were labeled using the LIVE/DEAD Fixable Aqua (405 nm) Dead Cell Stain Kit (BD Biosciences, San Jose, CA). EdU detection ...
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bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
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bioRxiv - Microbiology 2019Quote: ... ESAT6 peptide (1 μg/ml) or TB10 peptide (1 μg/ml) for 24 hours at 37°C before the addition of GolgiPlug (BD Pharmingen, San Diego, CA). The concentration of protein and peptides used were according to previous reports [19 ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 4% milk for 1 hour and incubated overnight at 4°C with shaking in the appropriate primary antibody against Nischarin (1:500, BD Biosciences, Cat No: 558262), GFP (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Systems Biology 2021Quote: ... xanthus strains were grown overnight at 32°C with vigorous shaking in CTTYE broth (1% Casein Peptone [Remel], 0.5% Bacto Yeast Extract [BD Biosciences], 10mM Tris (pH 8.0), 1mM KH(H2)PO4 ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated as described above and stained at 4°C (15 min) with fluorochrome-conjugated antibodies against surface markers of endothelial cells (CD31 (BD Biosciences, Franklin Lakes, USA) and myeloid cells (CD45 (BD Biosciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... It was retrieved from frozen glycerol stocks and cultivated for 24 h at 28 °C on tryptic soy agar (TSA, Becton, Dickinson and Company, USA), followed by culturing in 100 mL of tryptic soy broth (TSB ...
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bioRxiv - Immunology 2022Quote: ... the cells were fixed with 2% PFA and stored at 4°C until the samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada). Data analysis was performed using FlowJo v10.5.3 (Tree Star ...
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bioRxiv - Pathology 2019Quote: ... Samples were fixed with BD Biosciences stabilizing fixative and stored in the dark at 4°C until acquisition on a LSR Fortessa (BD Biosciences, San Jose, CA). Data were analyzed using FlowJo v 10.1r5 (FlowJo LLC ...
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bioRxiv - Cell Biology 2019Quote: ... Suspended cells were fixed using 70% ethanol and kept at −20°C for at least 24 hours prior to measuring DNA content using propidium iodine (PI) with RNAse (BD Biosciences, San Jose, CA). Approximately 50,000 cells were centrifuged at 1300 rpm for 5 minutes at 22°C ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) strain harboring pCOLD-1-EhPTEN1 was grown at 37°C in 50 ml of LB medium (BD Difco, New Jersey, USA) in the presence of 100 µg/ml ampicillin ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated at 90°C in a BBL GasPak™ 100 Holding Jar (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA) to avoid evaporation ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed and permeabilized for 30 min at 4 °C in the dark after extracelluar stainings using BD Cytofix/Cytoperm (Fixation and Permeabilization Solution, BD Biosciences, Cat: 51-2090KZ) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were blocked in 5% BSA and probed overnight at 4°C with the following primary antibodies and dilutions: p16 (1:200, #554079 BD Biosciences, San Jose, CA), p53 (1:200 ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... After 19h incubation at 37°C, the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity (MFI ...
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bioRxiv - Cell Biology 2022Quote: ... Cells grown on 96 well plates were fixed and permeabilized for 20 mins at 4°C with Fixation/Permeabilization solution (BD Biosciences, cat. no. 554714). Cells were washed 3 times in PBS ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Microbiology 2023Quote: ... incubated with 60 nM TMRE for 30 min at 37 °C, washed in PBS, resuspended in PBS-G (PBS, 6 mM glucose) and immediately analyzed by flow cytometry (BD FACS Canto II Instrument). In the case of oligomycin-treated cells ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4°C with primary antibodies: ALIX (BD Biosciences, 611620, 1:1000), calnexin (Santa Cruz ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min (RT) and incubated overnight (4 °C) with mouse anti-5-LOX (1:1000; # 610695; Lot: 9067817; BD Biosciences, Franklin Lakes, NJ) and rabbit anti-perilipin-2 (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin in PBS for 45 min at 37°C was followed by incubation with a primary antibody mix of anti-BrdU detecting IdU (mouse, #347580, BD, Franklin Lakes, NJ, USA) and anti-BrdU detecting CldU (rat ...
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bioRxiv - Immunology 2020Quote: Multiscreen ninety-six well plates were coated overnight with 100 μl per well of 5 μg/ml anti-human interferon-γ (IFN-γ) (B27; Becton, Dickinson and Company, Franklin Lakes, NJ) in endotoxin-free Dulbecco’s-PBS (D-PBS) ...
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bioRxiv - Cell Biology 2019Quote: ... were cultured under shaking conditions at 37°C with 5% CO2 atmosphere in THY-medium (Todd Hewitt Broth; Bacto; BD, complemented with 0.2% (w/v) yeast ...
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bioRxiv - Microbiology 2022Quote: ... All Bifidobacterium isolates were streaked for purity and all strains were grown at 37°C in RCM and de Man Rogosa and Sharpe (MRS) (BD Difco™, BD Biosciences, USA) media in an anaerobic chamber (Whitley DG250 ...
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bioRxiv - Neuroscience 2020Quote: ... After a second digestion of the retina-RPE complex in papain (20 U/ml) for ten minutes at 37°C the RPE cells were carefully peeled off and plated on Matrigel (BD Biosciences; 1:80 in DMEM)-coated glass coverslips in ‘Miller’ medium [DMEM supplemented with 5 or 20% fetal bovine serum (v/v) ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...
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bioRxiv - Immunology 2019Quote: ... Cells were stained for 30 min at 4°C under darkened conditions with the following fluorochrome labeled monoclonal antibodies: anti-CD4-AmCyan (Clone SK3, BD Biosciences, Franklin Lakes, Nj, USA), anti-CD25-APCCy7 (Clone M-A251 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Bacteria were cultured at room temperature (~25°C) in BBL Trypticase Soy Broth (TSB) or on Trypticase Soy Agar (TSA) plates (Becton, Dickinson and Company, MD, USA). For liquid cultures ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed with PBS and incubated for 20 hours at 4°C in primary antibody solution 1:3000 mouse anti-Ki67 (BD Biosciences, San Jose, CA, USA), 2% normal horse serum ...
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bioRxiv - Microbiology 2022Quote: ... Cells were grown in liquid MRS medium (De Man, Rogosa and Sharpe, DIFCO) at 37°C in anaerobic conditions using GasPak anaerobic jar (BD GasPak™ EZ container systems), without agitation ...
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bioRxiv - Systems Biology 2023Quote: ... All strains were reconstituted and grown anaerobically (6% H, 20% CO2, 74% N2) at 37°C in liquid Brain Heart Infusion medium (BHI; BD Bacto Ref:237500 Lot:1194923) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... purified gametocytes from DFsc7 or DFsc7;ΔRbpm1 lines were suspended in GMB at 4°C and sorted using a BD FACS AriaIII instrument (BD Biosciences, San Jose, CA, USA) based on their GFP and mCherry fluorescence intensity for male and female gametocytes ...
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bioRxiv - Neuroscience 2023Quote: ... Microdissected pieces were immunostained with antibodies to the following: (1) mouse anti-C-terminal binding protein 2 (pre-synaptic-marker, BD Biosciences, 1:200, Cat # 612044), (2 ...
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An oscillating MinD protein determines the cellular positioning of the motility machinery in archaeabioRxiv - Microbiology 2020Quote: ... the cells were grown at 45 °C or 42 °C in either rich YPC medium with Bacto™ yeast extract, peptone (Oxoid, UK) and Bacto™ Casamino acids (BD Biosciences, UK or Oxoid) or in selective CA medium containing only Bacto™ casamino acids 46,69 ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were incubated with phycoerythrin (PE)-conjugated Enpep antibody at 4°C for 60 min and then sorted by FACScan flow cytometry (Becton, Dickinson and company, Franklin Lakes, NJ).
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bioRxiv - Immunology 2020Quote: ... Cells were then surface stained for 30 min in the dark at 4°C with viability reagent (BD Horizon™ Fixable Viability Stain, BD Biosciences, San Diego, CA, USA) and a 29-color cocktail of monoclonal antibodies (mAbs ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were grown at 37°C with aeration (orbital shaking 220 rpm) in Lennox Broth (LB: 10 g/L BD Tryptone, 5 g/L BD Yeast Extract, 5 g/L NaCl), LBO 10 g/L BD Tryptone ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibody solutions for 5 days at 4°C: mouse monoclonal antiNMDAR1 (GluN1) (1:200; 556308, PharMingen, Becton, Dickinson and Company, Frankline Lakes, USA) or rabbit polyclonal antiNMDAa1 (GluN2A ...
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bioRxiv - Developmental Biology 2021Quote: ... a small number of images threw errors in MATLAB related to reading the image files (e.g. nuclei imaged too close to edge of frame) and were also removed (5 C mut, 5 BD mut, 3 CD mut, 1 sygl-1(ø)) ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by staining for 30 min at 4°C with the appropriate antibodies: PE-Cy7 rat anti-mouse CD45 (cat # 561868, BD Biosciences, clone 30-F11, 2 ng/μl), BV421 rat anti-mouse CD11b (cat # 562605 ...