Labshake search
Citations for Becton, Dickinson and Company :
2001 - 2050 of 2202 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... pyogenes M1T1 5448 strain (56) were grown aerobically at 37°C in Todd-Hewitt Broth supplemented with 2% yeast extract (THY, BD, France). Liquid and solid cultures of L ...
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bioRxiv - Immunology 2023Quote: ... 0.25X and 4X concentration of CD4 T cell-depleting antibody for 30 minutes at 37°C before surface staining with CD3 (clone SP34-2, BD Pharmingen), CD4 (Clone L200 ...
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bioRxiv - Microbiology 2023Quote: Giardia lamblia trophozoites (strain WBC6, ATCC 50803) were grown at 37°C without shaking in 16-ml screw-cap plastic tubes (BD Falcon) containing 12 ml modified TYI-S-33 medium supplemented with bovine bile ...
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bioRxiv - Cancer Biology 2023Quote: ... per well of U-bottom plate and coincubated for 4h at 37°C in the presence of NH4Cl and 1 µl Golgi PLUG (BD Biosciences). After that time cells were washed three times with 200 µl PBS and centrifuged (5 min ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae strains were derived from CEN.PK113-7D and grown at 30°C in Yeast Extract Phytone Dextrose media (YEPhD, 2% Difco™ phytone peptone (Becton-Dickinson (BD), Franklin Lakes ...
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bioRxiv - Bioengineering 2024Quote: ... Cells incubated with IL-10 at 37 °C were fixed and permeabilized for 20 min on ice with Cytofix/Cytoperm (BD Biosciences). Cells were washed twice in permeabilization buffer and further stained with anti-mouse IL-10 in permeabilization buffer for 30 min at on ice ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with staining buffer, fixed, and permeabilized (20 min, 4°C, dark) using Fixation and Permeabilization Solution (BD Biosciences). Cells were then stained with S100A9 (Clone ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with conjugated antibodies for 1 hour on ice: mouse anti-C-peptide antibody conjugated with Alexa Fluor 647 (BD Biosciences) and mouse anti-glucagon antibody conjugated with Bv421 (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... of anaerobic incubation at 37°C in a GasPak jar with an EZ Anaerobe Container System Sachets with Indicator (BD BBL), multiple colonies from each plate were inoculated into two (three ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... admixed with an equal number of HDFs expressing either S301D mutant or wildtype lamin A/C were re-suspended in 70 µl of Matrigel solution (BD Bioscience) and injected intradermally into the left and right side of the mouse back ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Microbiology 2023Quote: ... and Sharpe (MRS) plates supplemented with 1.5% agar at 37°C in anaerobic conditions (BD GasPak™ EZ Anaerobe Container System) overnight ...
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bioRxiv - Microbiology 2023Quote: ... Mtb strain H37Rv with plasmid pSMT3[Phsp60/DsRed] [9] was cultured at 37°C in complete Difco Middlebrook 7H9 Broth (BD Biosciences), supplemented with 10% ADC (BD Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for 15 min at 4 °C with a purified mouse anti-rat CD32 FcγII (clone D34-485, Rat BD Fc Block) to block unspecific binding ...
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bioRxiv - Immunology 2023Quote: ... The cells were cultured at 37°C and 5% CO2 for 72 hours and then the dye dilution was analyzed using a FACSVerse flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).
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bioRxiv - Developmental Biology 2023Quote: Hematopoietic cells were stained with the antibodies listed in Supp Table S1 for 20-30 min at 4°C and analyzed on a custom BD LSR Fortessa (BD Biosciences). For sorting ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 300 µg/mL DNase for 1 hour at 37°C to expose incorporated brdU and then stained with an APC anti-brdU antibody (1:50, BD Biosciences). 1X BD Perm/Wash in ddH2O was used as a staining and washing buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at 37°C and the single cell suspensions were filtered through a 70 µm cell strainer (BD Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for 30 minutes at 37°C washed and resuspended in ALDEFLUOR™ assay buffer and rapidly sorted on a FACSAria III cell sorting system (BD). 5×104 freshly sorted ALDHhi cells were seeded in seahorse XF24 plate in growth media and incubated overnight before seahorse analysis as described in analysis of extracellular flux section.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then fixed (eBioscience #00-8222-49) at 4 °C for 20 minutes and permeabilization (eBioscience #00-8333-56) before staining for intracellular IFNγ (BV650, BD #563854) and TNFα (BV421 ...
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bioRxiv - Microbiology 2023Quote: Stained cells were stored for up to 2 days at 4°C in the dark (TRAIL-R1) and flow cytometry was performed on a BD FACSCanto II (BD Biosciences). Raw data were exported to FlowJo v10.8.1 for analysis and plotting.
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
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bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were incubated overnight at 4°C in the dark and sample analysis performed the following day using an LSR FortessaTM X-20 device (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: Bacteria used in this work are listed in Supplementary Table 7 and were grown at 37 °C in brain and heart infusion (BHI) broth (with agitation) and agar media (BD Difco). Cell lines of human origin used in this work included JEG-3 trophoblasts (ATCC HTB-36) ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer instructions and stored at 4°C protected from light before acquisition on a FACSCalibur cytometer equipped with CellQuest Pro software (BD Biosciences) within 1 week of fixation ...
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bioRxiv - Genetics 2023Quote: ... 1.106 cells were incubated 20 min at 37°C in 100 μL solution BD cytofix/ cytoperm (BD PharmingenTM BrdU Flow Kit, 552598, BD Bioscience), washed with PBS and re-suspended in 700 µl of staining buffer (PBS with 3% SVF) ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 ΔPSA44 (lacks PSA biosynthesis locus) maintained in 25% glycerol at −80 °C were thawed on Brain Heart Infusion agar plates (BHI, BD BBLTM) supplemented with 5 µg/ml hemin (Alfa Aesar ...
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bioRxiv - Immunology 2023Quote: Groups: non-pregnant and pregnant E6-E8 BALB/c background Foxp3IRES-GFP females were used to isolate by FACS (BD FACSAria) several cell populations ...
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bioRxiv - Microbiology 2024Quote: ... Efficient induction of definitive endoderm was confirmed by flow cytometry analysis of c-Kit and CXCR4 co-expression using a FACS Symphony A5 instrument (BD Biosciences). NKX2.1+ distal lung progenitor cells were isolated via fluorescently-activated cell sorting (FACS ...
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bioRxiv - Biophysics 2024Quote: ... for three days at 20°C prior to being screened for Cy3 or Cy5 binding on a FACSVantage SE flow cytometer with FACSDiva Option (BD biosciences). For selection of scFvs against Cy3 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained with Fixable Viability Dye (20 min, 4°C, Fixable LiveDead, eBioscience) and then with a mix containing a brilliant stain buffer (BD Biosciences) and the surface markers for CD4+ T cells detection (CD3 and CD4 ...
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bioRxiv - Bioengineering 2024Quote: ... washed and stored at 4 °C for analysis the next day on a on a flow cytometry instrument (LSR Fortessa, BD Biosciences). Flow cytometry buffer was used to prepare Fc block and antibody solutions ...
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bioRxiv - Immunology 2024Quote: ... The samples were then stained with the fluorophore-conjugated antibodies or isotype controls in a flow cytometry buffer for 30 minutes at 4 °C in the dark and analyzed with the FACS symphony A1 (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... the cells were fixed with 2% (w/v) paraformaldehyde and stored at 4°C before analysis with FACS Celesta Cell Analyzer (BD Biosciences). Data were analyzed using FlowJo software version X.0.7 (Tree Star ...
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bioRxiv - Immunology 2024Quote: ... fluorescens J4 clinical isolates were grown for 1 day at 30°C in YM broth (BD Biosciences, San Diego, CA, USA). After gavages ...
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bioRxiv - Genomics 2024Quote: ... RoCKseq modified beads and unmodified beads used for controls were incubated with fluorescent oligos for 30 minutes at 46° C in BD Rhapsody lysis buffer (part number 650000064, BD RhapsodyTM Enhanced Cartridge Reagent Kit, BD 664887) with 1 M DTT (part number 650000063,BD RhapsodyTM Enhanced Cartridge Reagent Kit ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with a mouse pan α-synuclein primary antibody (Syn1, BD Biosciences, NJ, USA; #610786) diluted 1:5,000 in blocking solution ...
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bioRxiv - Microbiology 2024Quote: ... in BHI medium and grown at 37°C either with aeration for 8 h or overnight anaerobically (GasPak™ EZ Systems, BD). The cells were then removed from the supernatant by centrifugation (9,000 x g ...