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Citations for Becton, Dickinson and Company :
2051 - 2100 of 2202 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: Cytokine levels in clinical samples were assessed using the following commercially available kits: i) human Th1/Th2/Th17 Cytokine Kit (BD Bioscience, San Jose, CA, USA) based on the principle of cytometric bead array (CBA ...
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bioRxiv - Immunology 2022Quote: ... and the cell suspensions purity was higher than 90% as assessed by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... IgA and IgM antibodies were quantified in the CVL specimens using the human immunoglobulin cytokine bead array (CBA) flex set assay (BD Biosciences, San Jose, CA, USA). The Ig CBA assay was performed as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The following mouse and human monoclonal antibodies were utilized for virion capture assays: anti-PSGL-1 and anti-mouse IgG (BD Biosciences, Cat# 556053 and 557273) and anti-gp120s (clones 2G12 and PG9 acquired from the NIH ARP ...
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bioRxiv - Cancer Biology 2023Quote: ... to isolate GFP+ and GFP-populations from primary tumors after DAPI staining for live cells and from mice metastatic organs after labeling with human CD298-PE (BD, Cat#749741, clone P-3E10) and DAPI for human melanoma and live cells ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed twice with PBS as described above and the cell pellet was resuspended into 25 µl of FACS buffer (1% FBS in PBS) containing human FC block (BD Bioscience, # 564219; 1:200 dilution) and either 200 nM MLi-2 (synthesised by Natalia Shpiro ...
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bioRxiv - Immunology 2021Quote: ... Slides were treated for 10 minutes in H2O2 and after blocking in 10% goat serum in 0.1% Tween20 for 30 minutes and incubated overnight at 4°C with specific antibody diluted in PBS 0,1%Tween20: anti-mouse Ly6G (1A8, BD Pharmingen, cat 551459), or anti-mouse Ki67 (D3B5 ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and incubated overnight at 4° C with primary antibodies against Syn1 (mouse, 1:1000, BD Biosciences, #610786), pSer129 (rabbit ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were incubated with primary antibody solutions (using 5% milk or BSA in TBST) overnight at 4°C at the respective dilutions: E-Cadherin (BD, #610181,1:5000), Vimentin (Abcam ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed once with wash buffer 1 and incubated for two days at 4°C with mouse anti-SR antibody (1:1,000; BD Biosciences; cat #612052). Sections were washed with wash buffer 2 (2% BSA ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Bioengineering 2020Quote: ... was applied for 30 minutes at 4°C and stained cells were resuspended in FACS buffer before flow analysis on a BD FACSCanto™ II (BD Biosciences). Gating and flow data were processed on FlowJo (FlowJo LLC).
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were labeled with murine antibodies against CD3 (BV650) ...
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bioRxiv - Cancer Biology 2020Quote: ... then probed with the following primary antibodies in 5% milk/TBS-T overnight at 4 °C: SREBP-2 (1:250; BD Biosciences, 557037), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2022Quote: ... washed three times with RPMI and incubated for 30 mins at 37°C in RPMI containing Dispase (BD Cornig, 1:10 dilution) and 30 μg/μl DNase I with continuous and gentle agitation on a rotating shaker ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were also stained with mouse anti-ssDNA (DSHB University of Iowa) for 75 minutes at 37°C and detected with goat anti-mouse BV480 (BD Biosciences, 564877) for 45 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... (CGSC#7636) (Baba et al., 2006; Datsenko and Wanner, 2000) were grown at 37 °C in Luria-Bertani (LB) broth (BD, Sparks, MD). E ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies (Claudin-5, Abcam Ab131259, 1:1000; Collagen IV, Abcam Ab6586, 1:500; Hemoglobin, R&D Systems G-134-C, 1:300; P62, BD Biosciences 610833 ...
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bioRxiv - Microbiology 2019Quote: ... Pure bacterial cultures were revived from −80°C in tryptic soy broth plus 0.6% yeast extract (TSBYE, Bacto™, BD, Spark, USA). Cultures were plated onto TSAYE agar (TSBYE plus 1.5% agar (Bacto ...
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bioRxiv - Immunology 2019Quote: ... slides were incubated for 50 min at 25 °C with different combinations of the following anti-mouse Abs (Thermo Fisher Scientific, Biolegend, BD Biosciences): Alexa Fluor 488-labeled anti-CD3 (HM3420 ...
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bioRxiv - Immunology 2019Quote: Cells were harvested by centrifugation at 400 g for 5 minutes at 4°C followed by staining with fixable viability stain (BD Horizon FVS700) for 10 minutes on ice in PBS ...
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bioRxiv - Microbiology 2021Quote: ... All strains were grown aerobically on an orbital shaker (150 rpm) at 37 °C in Luria-Bertani (LB) broth (BD Difco, US) overnight ...
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bioRxiv - Microbiology 2021Quote: ... The cMRS agar plates were incubated at 30 °C under aerobic or anaerobic conditions (BD BBL GasPak system (BD, Franklin Lakes, NJ) for 48 h ...
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bioRxiv - Immunology 2019Quote: ... Unfractionated and lineage-depleted (Lin-) cells were labelled with phycoerythrin (PE) conjugated antibodies for CD11b and c-Kit (BD Biosciences, Mississauga, ON), and analyzed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 h of incubation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... reduced to a single-cell suspension and seeded into a 96-well plate at a density ranging between 1000 – 20 c/w in mTesRTM1 supplemented with 1 nM ROCK inhibitor (BD Biosciences, 562822). Alternatively ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed using 4% PFA for 15 minutes at 4°C and permeabilized for intracellular cytokine staining using Perm/Wash Buffer (BD Perm/WashTM). For intracellular staining ...
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bioRxiv - Cell Biology 2021Quote: ... C-peptide and Glucagon labelled cells were detected on an LSRII flow cytometer with FACSDiva™ Software (BD Biosciences, San Jose, CA) using the 405- ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for 15 min at 37°C before incubation with primary antibodies Alexa Fluor 647 Rat Anti-mouse CD31 (BD Pharmigen cat:563608) for CD31 cell labeling and Alexa Fluor 647 Rat IgG2a,k Isotype control (BD Pharmigen cat:557690) ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug™, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 monoclonal antibody (mAb ...
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bioRxiv - Immunology 2020Quote: Cryopreserved lymphocytes from BAL were thawed and stimulated with H1N1pdm09 (MOI 1) or medium as a control for 18 hours at 37°C prior GolgiPlug (BD Biosciences, UK) addition as per manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... Cells were resuspended in PBS/5%FBS then fixed in an equal volume of 1% paraformaldehyde and stored in the dark at 4°C prior to analysis on a BD LSR FortessaTM or LSR2 flow cytometer (BD Biosciences, UK). Data were analysed using FlowJo software (FlowJo LLC ...
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bioRxiv - Immunology 2020Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop™, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed at 4 °C for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 °C for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer ...
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bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...
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bioRxiv - Neuroscience 2022Quote: ... GAS 2006 was cultured under static conditions at 37°C for 17 h in THY medium (Todd-Hewitt broth (Bacto BD, Cat# 249240) containing 1.5% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... for 10 min at 37 °C and 5 % CO2 before they were stained with Fixable Viability Stain 510 (1:100, #564406, BD Horizon, USA) for 15 min at room temperature (RT ...
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bioRxiv - Immunology 2022Quote: ... Next cells were fixed using BD Cytofix Fixation buffer for 10 minutes at 37°C and permeabilized using ice-cold Perm buffer III (BD Biosciences, 558050) for 30 minutes on ice ...
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bioRxiv - Physiology 2022Quote: ... Samples were stored at 10-15°C in 2 mL K2-EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 15 to 45 min later.
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bioRxiv - Physiology 2022Quote: ... The sample was stored at 10-12 °C in 2 mL EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 0.5-2.5 h later ...
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bioRxiv - Microbiology 2024Quote: Lung cytokines were quantified from the supernatant of thawed organ homogenates (stored at - 80°C) from each experimental timepoint using the BD Mouse Th1/Th2/Th17 Cytometric Bead Array (CBA) Kit (BD Biosciences, 560485). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with the following conjugated antibodies (1/100 dilution in PBS) for 15 minutes at 4°C: CD44 (PE, BD Biosciences 555479), EpCAM (FITC ...