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Citations for Becton, Dickinson and Company :
2101 - 2150 of 2202 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C in a TC-7 roller drum (NB, Canada) at 240 rpm or on Lysogeny Broth (LB; BD Difco, Canada) agar plates ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cells were washed once in FACS buffer before fixation for 20 min at 4°C using the BD Cytofix/Cytoperm solution and permeabilization using BD Perm/Wash™ buffer (BD Biosciences). Permeabilized cells were incubated for 30 min with antibodies (diluted 1:200 in BD Perm/Wash ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... on ice and enzymatically digested with agitation for 30 minutes at 37°C using the BD TuDoR™ dissociation reagent (BD Biosciences). The obtained single-cell solution was sieved through a 70 µM cell strainer (Corning ...
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bioRxiv - Immunology 2023Quote: The plate was incubated 6 hours at 37 °C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 hours of culture ...
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bioRxiv - Cancer Biology 2023Quote: ... treatment was performed at 100μg/ml concentration in dark at 4°C for an hour and analyzed by FACS Calibur flow cytometer and processed using Cell Quest software (BD Biosciences, USA).
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Genomics 2023Quote: Trypsinized or MACS sorted cells were incubated at 4°C for 20 min with APC-conjugated anti-CD56 antibodies (1:40, BD Pharmingen #555518) or isotype control (1:40 ...
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bioRxiv - Genomics 2023Quote: ... then washed three times with DPBS before permeabilization in ice cold Phosflow Perm Buffer III for 10mins at -20°C (BD Bioscience #558050). Before incubation with primary antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... murinus KD6 was incubated aerobically at 37°C overnight in 10 mL MRS media (Difco Lactobacilli MRS agar, BD Biosciences, cat# 288210). Cultures were centrifuged 10 min at 2500 rpm ...
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bioRxiv - Microbiology 2024Quote: ... The urine samples were obtained in sterile BD Vacutainer® urine C&S Preservative Tubes (BD, 1 Becton Drive, Franklin Lakes. NJ). Specifically ...
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bioRxiv - Immunology 2024Quote: ... Surface staining was carried out for 30 minutes at 4°C with the following fluorochrome-labeled anti-mouse Abs: APC/Cy7-CD45 (BD Bioscience, #557659), PE/Cy5-B220 (eBioscience ...
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bioRxiv - Neuroscience 2024Quote: ... cells were blocked with Superblock blocking buffer in PBS/ 0.1% Triton-X for 1 h and then incubated with the following primary antibodies overnight at 4 °C: α-CD11b (1:100; BD Biosciences) and α-Iba1 (1:200 ...
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bioRxiv - Biophysics 2024Quote: ... transferred in a V-bottom 96-well plates and co-cultured with K562 cells (ATCC) at 1:1 effector-to-target ratio in the presence of mouse anti-human CD107a Alexa Fluor 647 (BD Bioscience, clone H4A3, 2 uL/well) for 6 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: Low-passage virulent Leptospira interrogans serovar Pomona was cultured at 28°C in Ellinghausen–McCullough–Johnson–Harris (EMJH) medium (BD Difco™, USA) supplemented with 10% bovine serum albumin (BSA) ...
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bioRxiv - Bioengineering 2019Quote: ... and Anoxybacillus flavithermus strain WK1 (DSMZ, Germany) cells were grown at 55°C in Caso Broth (15g/L of peptone from casein (BD, cat no. 211921), 5g/L of peptone from soybean (Sigma-Aldrich 70178-100G) ...
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bioRxiv - Cell Biology 2019Quote: ... for 2 hours at room temperature followed by overnight incubation at 4°C with anti-PECAM-1 (MEC13.3, BD Bioscience #550274, 1:250) diluted in blocking buffer ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...
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bioRxiv - Microbiology 2022Quote: ... All Bifidobacterium isolates were streaked for purity and all strains were grown at 37°C in RCM and de Man Rogosa and Sharpe (MRS) (BD Difco™, BD Biosciences, USA) media in an anaerobic chamber (Whitley DG250 ...
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bioRxiv - Microbiology 2022Quote: Strains were streaked from -80°C freezer stocks (frozen in Brain Heart Infusion [BHI] broth with 15% glycerol) onto Brain Heart Infusion agar (BHIA; BD, Franklin Lakes, NJ), followed by incubation at 37°C for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Mice recovered at 32°C (Warm Air System, Safety Cabinet Version red, VetTech, UK) until spontaneous movement and received 0.8mg/kg subcutaneous (BD Microlance 27G 13mm needle) atipamezole to reverse the ɑ-agonist into the neck scruff.
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bioRxiv - Immunology 2020Quote: ... Pellets were then stained with a panel of antibodies for 30 minutes (4°C) followed by washes in PBS before analysis by flow cytometry (BD, Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated for 30 min at 4 °C with a saturating concentration of anti-CD24a monoclonal antibody (eBioscience, clone M1/69) and anti-CD56 monoclonal antibody (BD Biociences, clone 809220) in the presence of anti-CD16/CD32 (eBioscience) ...
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bioRxiv - Cell Biology 2020Quote: ... cerevisiae cells were grown at 25°C in YPD liquid medium containing 1% (w/v) Bacto yeast extract (BD Biosciences, San Jose, CA), 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cultures were routinely grown in lysogeny broth (LB) broth at 37°C with aeration or on LB solidified with 1.5% agar (BD Biosciences, San Jose, CA). When stated ...
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bioRxiv - Immunology 2020Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... mice were euthanized and tumors were quickly harvested and fixed for 14-16 hr at 4&C in BD Cytofix/Cytoperm (BD Bioscience, Cat #: 554722) diluted 1:4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... In-between incubation steps, cells were washed (300 x g, 10 min, 4°C) with 40 mL of BD Cell Wash (BD Biosciences, Allschwil, Switzerland). In order to block Fc receptors ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the samples were fixated and permeabilized for 20 min and 60 min at 4 °C in the dark using BD Cytofix/Cytoperm™ solution and BD Perm/Wash™ solution (BD Biosciences), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were incubated in the 9-well plates with 100 μL per plate overnight at 4°C: CtBP2 mouse IgG1 (BD Biosciences 612044; RRID:AB_399431), GluA2 mouse IgG2a (Millipore MAB397 ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... cells were restimulated ex vivo for 4-5h at 37 °C in complete RPMI medium (RPMI supplemented with 2mM L-Glutamine and 10% fetal calf serum (FCS)) with GolgiPlug (BD, 1:1,000 dilution), phorbol 12-myristate 13-acetate (50ng/ml ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... overnight and then stimulated with 1 nM N4 in the presence of vehicle or Compound 182 (1 μM) for 4 h at 37°C in complete T cell media supplemented with BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were then stained with fluorophore-conjugated antibodies to cell surface CD8 and intracellular IFNγ ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Immunology 2023Quote: ... pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL, clone: 145-2C11, BD Biosciences, San Diego, CA, USA) and supplemented with soluble anti-CD28 antibodies (2ug/mL clone ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Genomics 2023Quote: ... and half of the remaining chromatin was rotated overnight at 4°C with 4.5 µg of anti-p53 (BD Biosciences #554293, Lot #9046678). The other half of the remaining chromatin was also rotated overnight as a no-antibody control ...