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Citations for Becton, Dickinson and Company :
1851 - 1900 of 2202 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... CECs were incubated overnight at 4°C with primary antibodies mouse monoclonal anti-CD166 [3A6] (1:200 dilution, BD Biosciences), rabbit polyclonal anti-ZO1 (1:100 dilution ...
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bioRxiv - Pathology 2023Quote: ... Cells were then incubated for 20 minutes at 4°C with the following antibodies (from BD Biosciences unless stated otherwise): BV605 rat anti-mouse CD19 (clone 1D3) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated overnight at 4 °C with primary antibodies anti-E-cadherin (mouse clone 36 BD610182, BD, Heidelberg, Germany), anti-paxillin (mouse clone 5H11 AHO0492 ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 3 hours at 37°C with 5% CO2 in R10 buffer supplemented with 0.33 μl/ml GolgiStop (BD Biosciences) and 15 μg/ml DNase I ...
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bioRxiv - Microbiology 2023Quote: ... 1x105 CFU bacteria were incubated for 48h at 37°C in 0.2 mL cation-adjusted Mueller-Hinton broth (CAMHB) (BD Biosciences) containing increasing concentrations of antibiotic (0.25 mg/L to 256 mg/L for Nafcillin and Oxacillin ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated at 37°C for 4 hours after which they were fixed and permeabilized with the Transcription Factor Buffer Set from BD. γH2AX was detected with PerCP/Cyanine5.5 anti-H2A.X-Phosphorylated (Ser139 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in 3% BSA/PBST (0.1% Triton X-100 in PBS) and incubated with the samples at 37°C for 1 h: mouse anti-BrdU (BD Biosciences 347580 ...
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bioRxiv - Microbiology 2023Quote: ... Individual colonies were picked for overnight growth at 37◦C in 1.6mL LB (Miller) broth (BD, Franklin Lakes,NJ, USA) each ...
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bioRxiv - Microbiology 2023Quote: ... aureus and Corynebacterium strains were cultured overnight at 37°C with shaking in brain-heart infusion broth (BHI; BD Biosciences) unless otherwise noted ...
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bioRxiv - Biophysics 2024Quote: Bacterial strains stored at −80 °C were plated on Mueller–Hinton agar (MHA, BD Difco, BD, Franklin Lakes, NJ USA) and incubated at 37 °C overnight prior to use ...
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bioRxiv - Biophysics 2024Quote: Bacterial strains stored at −80 °C were plated on Mueller–Hinton agar (MHA, BD Difco, BD, Franklin Lakes, NJ USA) and incubated at 37 °C overnight prior to use ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and resuspendend in cRPMI and kept at 4°C for sorting on a FACSAria III (BD Bioscience). γδ T cells were sorted based on scatter ...
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bioRxiv - Immunology 2023Quote: ... at 37 °C for 10 min immediately after the harvest and permeabilized with BD PhosflowTM Perm Buffer III (BD Biosciences) on ice for 30 min ...
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bioRxiv - Physiology 2024Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
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bioRxiv - Immunology 2024Quote: ... stained with 2.5 μM cisplatin for 5 min at 25°C and fixed using transcription factor buffer set (BD Biosciences), followed by intracellular staining for 60 min at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... RoCKseq modified beads and unmodified beads used for controls were incubated with fluorescent oligos for 30 minutes at 46° C in BD Rhapsody lysis buffer (part number 650000064, BD RhapsodyTM Enhanced Cartridge Reagent Kit ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and stained for 20 min at 4°C with mAbs CD4-APC-Cy7 (BD, clone RPA-T4), CD8-BV605 (clone RPA-T8) ...
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bioRxiv - Molecular Biology 2024Quote: ... and labeled overnight at 4°C in 1% BSA in PBST containing mouse anti-G3BP1 (BD Biosciences, 1:1000 dilution) and rabbit anti-Caprin-1 (Proteintech ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then incubated overnight at 4°C with a pair of mouse and rabbit antibodies: a mouse antibody recognizing L1 (BD Biosciences ...
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Inhibition of CELA1 Improves Septation in the Mouse Hyperoxia Model of Impaired Alveolar DevelopmentbioRxiv - Developmental Biology 2024Quote: One million cells from each lung were incubated (4°C, 30 minutes) with anti–mouse CD16/CD32 (BD Biosciences, 553142) to block Fc receptors ...
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bioRxiv - Microbiology 2024Quote: ... All ESKAPE strains were grown at 30-37°C using Luria broth/agar (BD Difco, Fisher Scientific #DF0145-17-0) for 15-24 h.
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bioRxiv - Microbiology 2024Quote: ... guizhouense strain NJAU 4742 (NCBI accession PRJNA314460) were grown at 28 °C in Potato Dextrose Agar (PDA, BD Difco, USA) medium for 7 days with light ...
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bioRxiv - Microbiology 2024Quote: Bacteroides strains were grown anaerobically for 24 hours at 37 °C in 500μl of brain heart infusion broth (BD Biosciences) supplemented with 5 μg/ml hemin and 0.5 μg/ml vitamin K (BHI-S ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by stimulation for the appropriate time at 37°C with pre-warmed anti-hamster IgG1 antibody (5µg ml-1; G94-56, BD Pharmingen). Ex vivo expansion of B cells was performed using recombinant murine IL-4 (100 u ml-1 ...
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bioRxiv - Immunology 2024Quote: ... incubated at 37 °C for 30 min and analysed by flow cytometry (Fortessa X-20, BD Biosciences, San Jose, CA).
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bioRxiv - Cancer Biology 2021Quote: Cells from the cell line U3065 obtained from the Human Glioma Cell Culture (HGCC) resource [35] were suspended in stem cell medium and plated on 384 well plates (BD Falcon Optilux TC #353962) coated in laminin ...
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bioRxiv - Cancer Biology 2020Quote: ... Enumeration of human CD45+ cells in spleen and bone marrow was performed by flow cytometry after staining with BV605-conjugated human-specific CD45 antibody (clone HI30, BD Biosciences, San Jose, CA) using a BD LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions at 5 × 107 cells per ml were stained with a cocktail of fluorochrome conjugated human antibodies (BD Biosciences, San Jose, CA), anti-human IgG APC ...
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bioRxiv - Bioengineering 2020Quote: ... Coated beads were then incubated with anti-human CD63 (eBioscience, San Diego, CA) and analysed with a BD AccuriTM C6 (BD Biosciences, San Jose, CA).
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated at 1×106 cells/ml with 10 μg/mL soluble Leishmania antigen (SLA) plus 1 μg/mL purified NA/LE anti-human CD28 (clone CD28.2, BD Biosciences, San Jose, CA, USA) at 37°C / 5% CO2 for 15 h and subsequently incubated in the presence of brefeldin A (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Biochemistry 2020Quote: ... and TNF in the supernatants was determined by using Cytometric Bead Array (CBA) - Human Inflammatory Cytokines Kit (551811, BD Biosciences, San Jose, CA, USA), that allows the determination of the indicated human cytokines simultaneously ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Whole blood was collected from human volunteer donors with informed consent in 8 to 10 BD Vacutainer CPT 8 ml tubes (BD Biosciences, San Jose, CA). The blood samples were mixed immediately prior to centrifugation by gently inverting tubes 8-10 times ...
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bioRxiv - Immunology 2021Quote: ... harvested and stained for flow cytometry as described (37) using antibodies to human surface antigens: CD4-APCH7 (SK3, BD Biosciences Franklin Lakes, NJ, USA), CD8-PerCP (SK1 ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μl of each sample was analysed in duplicates using the BD™ CBA Human Soluble Protein Master Kit (#556264; BD Biosciences, CA, USA) alongside the BD™ CBA Human Flex Sets for interleukin 1ß (IL-1ß ...
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bioRxiv - Immunology 2023Quote: ... a total of 50 ml of whole human blood was collected by a trained phlebotomist from healthy donors in BD Vacutainer® EDTA tubes (BD Vacutainer®, USA). This was previously approved by the RVC’s Ethics and Welfare Committee (URN 2019 1916-3).
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bioRxiv - Molecular Biology 2023Quote: ... Evaluation of proliferation in tissues using antibodies against Ki67 or BrdU (anti-human Ki67 and anti- BrdU monoclonal antibodies; Thermo-Fisher Scientific and BD BioSciences, USA, respectively) was performed as previously described {GarciaMelendrez:GKcY05su } ...
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bioRxiv - Cancer Biology 2023Quote: Ba/F3 cells expressing human MPL protein that were either cultured normally or treated with drugs were harvested and stained for surface MPL using PE-conjugated anti-human MPL antibody (BD Pharmingen 562159,1:50 dilution) in staining buffer for 30 min at 4°C.
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bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% bovine serum antigen) and stained with either 1:20 anti-HLA-A,B,C-PE (clone W6/32, BD Biosciences), 1:100 anti-β2m-FITC (clone 2M2 ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and consecutively sorted using a SONY SH800S or a FACS Aria Cell Sorter (BD Biosciences) into NCAM1- and NCAM+ populations ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and sorted into 384-wells SORTseq plates using a FACS Aria Cell Sorter (BD Biosciences) and directly stored at −80°C until further processing.
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bioRxiv - Microbiology 2020Quote: ... washed with cold PBS by centrifugation (5 min, 550g at 4°C) and analysed with a FACS BD AccuriTM C6 (BD Biosciences) instrument ...
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bioRxiv - Microbiology 2020Quote: ... cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Pacific Blue; BD Biosciences), anti-CD4 (L200 ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were incubated for 30 min in the dark at 4°C after adding relevant antibodies (BD biosciences, Franklin Lakes, NJ). A FACSCalibur system (BD biosciences ...
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
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bioRxiv - Cell Biology 2022Quote: Cells harvested at different time points of reprogramming were stained in FACS buffer for 30 min at 4°C and washed with FACS buffer prior to acquisition with LSR Fortessa (BD Biosciences) cytometer ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...