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Citations for Becton, Dickinson and Company :
1651 - 1700 of 2202 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... spleen and bone marrow were isolated and evaluated for the percentage of human CD45+ cells by flow cytometry (CD45-PerCP-Cy5.5, Biolegend Cat: 304028; BD FACS Canto II). Populations were defined and analyzed using FlowJo software (Flowjo v10.7.1 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected after 6 days of co-culture to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... a panel of four fluorescent markers was employed including fluorescein isothiocyanate (FITC) mouse anti-human CD40 (BD Biosciences; San Jose, CA, USA), phycoerythrin (PE ...
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bioRxiv - Immunology 2022Quote: Secretion of CXCL10 (IP-10) into the medium was quantified 24 h after stimulation by ELISA (Human IP-10; BD OptEIA, 550926). Briefly ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Immunology 2023Quote: ... were diluted 1:50 in PBS-10% FBS and IL-2 concentration was determined by ELISA assays using the OptEIA Human IL-2 ELISA commercial kit (BD Biosciences, USA) and revealed with TMB substrate (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cell Biology 2023Quote: ... VE-cadherin was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD144 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... A target primer panel was constructed by combining a commercial predesigned Human T Cell Expression Primer Panel (BD Biosciences, 259 target primers) with our custom-designed primer panel (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: The purity of each NK population was checked before and after cell sorting and routinely by flow cytometry with anti-human CD16-BV421 (clone 3G8, BD Pharmagen, France), anti-human CD56-PeCy7 (clone B159 ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and co-cultured with human CD8+ T cells at 1:1 E:T ratio in the presence of anti-CD107a-APC mAb (BD Biosciences, CA, USA). After 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Cancer Biology 2024Quote: ... Myelin-depleted single-cell suspensions obtained from high grade glioma samples were sequentially labeled using the BD Human Sample Multiplexing Kit (Sample-tag) (BD Biosciences, #633781), the sorting antibody CD45-APC (#560973 ...
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bioRxiv - Immunology 2021Quote: ... Single-cell suspensions were incubated for 20 min at 4°C with anti–mouse FcγRIII/II (2.4G2; BD Pharmingen) prior to staining for 1 h at 4°C with the following antibodies from BioLegend (most ...
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bioRxiv - Microbiology 2021Quote: ... creating either N- or C-terminally tagged GAL4 activation domain (AD/prey) or GAL4 DNA-binding domain (BD/bait) fusion proteins ...
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bioRxiv - Developmental Biology 2020Quote: ... Then the c-KIT positive cells were isolated by cell sorting using a BD FACSAria cell sorter (BD Bioscience) at the KUMC flow cytometry core.
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated overnight at 4°C with antibodies against E-Cadherin (CDH1) at 1:200 (BD Biosciences, 610181) and BAP1 at 1:100 (Cell Signaling ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed with −20°C 75% ethanol overnight and then stained with PI/RNase staining buffer (BD pharmingen) for 15 minutes at room temperature and analyzed with flow cytometry (Beckman Coulter ...
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bioRxiv - Bioengineering 2019Quote: ... (DSM 2641) cells were grown at 55°C in Nutrient Broth (5g/L of peptone (BD, cat no. 211677), 3g/L of beef extract (BD ...
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bioRxiv - Biochemistry 2020Quote: ... Yeast strains were cultured at 30 °C in complex yeast extract peptone dextrose (YPD, all components from BD Diagnostics) medium or synthetic defined medium (SDM ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were stained overnight at 4°C with the following primary antibodies diluted in CytoPerm/Wash buffer (BD Biosciences): recombinant rabbit anti-CEA (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... were grown shaking at 37°C in Luria-Bertani (LB) medium (25 g Difco LB broth [BD] per liter). Growth media were solidified with 1.5% agar (15 g Bacto agar (BD ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated for 15 min at 4°C and analyzed on a FACSCanto II flow cytometer (BD Biosciences) using BD FACSDiva v6.1.1 software ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated for 72 h at 4°C in mouse anti-PKCδ (1:1000, BD Biosciences, 610397), rabbit anti-Phospho-c-Fos (1:2000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... yeast strains were cultured at 28 °C in complex yeast extract peptone dextrose (YPD, all components from BD Biosciences) medium ...
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bioRxiv - Microbiology 2020Quote: ... coli strains were routinely grown aerobically at 37 °C under aerobic conditions with shaking (200 r.p.m.) in Luria-Bertani (LB) medium (BD, USA), supplemented with 25 µg mL−1 chloramphenicol (Merck ...
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bioRxiv - Microbiology 2021Quote: ... bovis BCG (strain Pasteur) was grown at 37°C in Middlebrook 7H9 media (Becton Dickinson, BD, New Jersey, USA) supplemented with 0.5% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37 °C in anaerobic chambers with GasPak™ EZ Gas Generating Container System (BD Diagnostics) to maintain the anaerobic condition during the incubation for indicated time points ...
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bioRxiv - Bioengineering 2022Quote: ... and anti-hJagged2 PE) for 1 hour at 4°C and analyzed by flow cytometry (BD Accuri C6 plus); however ...
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bioRxiv - Cancer Biology 2022Quote: ... and then fixed using the BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Biosciences, 554722; 20 minutes at 4°C). Cells were then washed twice in 1x BD Perm/Wash buffer ...
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bioRxiv - Immunology 2020Quote: ... The third staining at 4 °C for 30min involved either: Streptavidin-APC (eBioscience) and/or Streptavidin-PE (BD Biosciences) to target the Strep tag of RBD ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... cells were resuspended in Fix/Perm Solution for 20min at 4°C to fix and permeabilize the cells (BD Bioscience Cytofix/Cytoperm Cat # ...
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bioRxiv - Microbiology 2022Quote: ... then washed and stored at 4°C in the dark until analysis using a FACSCelesta cell analyzer (BD Biosciences) configured with 405 nm ...
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bioRxiv - Microbiology 2022Quote: ... They were cultivated on PDA plates at 25°C for growth and in potato dextrose broth (BD Difco™) culture for genomic DNA extraction as previously reported41 ...
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bioRxiv - Immunology 2022Quote: ... washed with PBS and incubated for 30 minutes at 4°C in the dark with Brilliant Stain Buffer (BD) and the fluorescently-conjugated antibodies ...
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... Bacterial cells were grown at 37°C with reciprocal shaking at 180 rpm in LB Miller medium (BD Biosciences) or MOPS minimal medium (Neidhardt et al ...
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bioRxiv - Immunology 2019Quote: ... FCS in PBS and were stained for 1 hour at 37°C with Vγ5 TCR-FITC (clone 536; BD), MHCII-AF647 (I-A/IE ...
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bioRxiv - Genetics 2020Quote: ... at 37°C / 5% CO2 for 15 h and subsequently incubated in the presence of brefeldin A (BD Biosciences) at 37°C with 5% CO2 for up to 4 h ...
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bioRxiv - Immunology 2019Quote: ... pylori was cultured at 37°C under microaerophilic conditions in Difco Columbia blood agar (BD Biosciences, San Jose, CA) plates supplemented with 7% of Horse laked blood (Lampire Biological Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated overnight at 37°C and then bacteria were harvested by adding 1.0 ml of Buffered Peptone Water (BPW, BD) to the surface of the plate and gently scraped using an L-shaped plate spreader ...
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bioRxiv - Synthetic Biology 2020Quote: ... Yeast strains were cultured at 30 °C in complex yeast extract peptone dextrose (YPD, all components from BD Diagnostics) medium or synthetic defined medium (SDM ...
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bioRxiv - Immunology 2020Quote: ... for 45 minutes at 4°C in FACS buffer containing 0.5 mg/mL anti-mouse CD16/CD23 (2.4G2) (BD Biosciences). Flow cytometry was performed on an LSR-II with BD FACSDIVA software (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... specific antibodies were bound with polyclonal anti-mouse Ig-FITC (1:300, 4°C, 20 min incubation; BD Biosciences), anti-mouse IgG1-APC (1:300 ...
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bioRxiv - Immunology 2020Quote: ... followed by intracellular staining (30 min at 4°C) using a cocktail of the following antibodies purchased from BD, Biolegend ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was prepared from all the isolates grown overnight at 37°C in tryptone soy broth (BD Biosciences), using bacterial DNA kits (Omega Bio-Tek) ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies for flow cytometry were: rat anti-C-peptide-Alexa Fluor 647 (1:200, 565831, BD Pharmigen) and mouse anti-Glucagon-BV421 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... for 15 min at 37 °C and counted using a BD Accurri flow cytometer (BD Bioscience, Franklin Lakes, NJ). The ratio between alive and dead cells was calculated by staining the same diluted biofilm suspension using the LIVE/DEAD® BacLight™ Bacterial Viability and Counting kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... lactis strains were grown at 30 °C in M17 liquid medium (BD - Becton, Dickinson and Company, Franklin Lakes, NJ) supplemented with 0.5 % glucose (GM17).