Labshake search
Citations for NanoTemper :
251 - 300 of 1068 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Measurements were performed at 22 °C using medium MST power and 100% excitation power settings on the MO.control data collection software (NanoTemper Technologies GmbH). The data were analyzed and fit using the MO.Affinity Analysis software (NanoTemper Technologies GmbH ...
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bioRxiv - Developmental Biology 2023Quote: ... microscale thermophoresis (MST) assays were carried out by using a Monolish NT.115 (NanoTemper Technologies). His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper ...
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bioRxiv - Cell Biology 2023Quote: ... The Monolith NT.115 microscale thermophoresis instrument with premium coated capillaries (NanoTemper, Munchen, Germany) was used to measure binding curves ...
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bioRxiv - Cell Biology 2023Quote: ... Microscale thermophoresis was performed using a Monolith NT.115 (NanoTemper). Data were analyzed by MO Affinity Analysis software ...
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bioRxiv - Microbiology 2023Quote: ... thermal shift assays were performed with nanoDSF integrated in the Prometheus NT.48 (NanoTemper Technologies GmbH, Germany). Protein solutions of 75-100 µM of YopR WT ...
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bioRxiv - Biochemistry 2023Quote: ... microscale thermophoresis (MST) assays were performed on a Monolith X (Nanotemper Technologies) using standard capillaries (MO-K022 ...
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bioRxiv - Biochemistry 2023Quote: ... using a Prometheus NT.48 (NanoTemper, Germany) as previously described (33) ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 10 µL of each sample was loaded into Prometheus NT.48 Series nanoDSF high sensitivity capillaries (NanoTemper, Germany) and melting scans were performed using the Pr.ThermControl software (NanoTemper ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then briefly spun-down using a tabletop centrifuge and incubated in the dark at room temperature for one hour prior to being loaded into Monolith Standard Capillaries (NanoTemper, Germany) and microscale thermophoresis measurements were performed using the MO.Control Software with a medium laser power and fluorescence auto-detection ...
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bioRxiv - Biochemistry 2023Quote: ... Data were analyzed by MO.Control and MO.Affinity software (NanoTemper). The experiments were performed in triplicate.
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bioRxiv - Biochemistry 2023Quote: ... The measurements were performed on a Monolith NT.115 (NanoTemper) using standard capillaries at 25 °C with 80% MST power ...
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bioRxiv - Biochemistry 2023Quote: ... over 12 serial diluted Monolith NT.115 premium capillaries (NanoTemper) with 10 μl samples ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing varying concentrations of protein were loaded into glass capillaries and applied to the nanoDSF device Prometheus NT.48 (Nanotemper). The samples were heated from 20 to 90°C with a slope of 1.5°C/min and the unfolding of the proteins was observed by detecting shifts in the fluorescence at 330 and 350 nm ...
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bioRxiv - Bioengineering 2023Quote: ... and absorption at 330 and 350 nm was measured on a Prometheus NT.48 instrument (NanoTemper) in the temperature range 30-95°C with a temperature slope of 1.5°C/min ...
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bioRxiv - Immunology 2023Quote: ... Each sample was transferred into a capillary and measured from 35 °C to 95 °C using a Tycho N6T (Nanotemper). Data were analyzed in Prism (GraphPad Software).
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bioRxiv - Cell Biology 2023Quote: His-Tag labeling Kit RED-tris-NTA 2nd generation (MO-L018, Nanotemper) was used to label His-tagged Bax purchased from Creative BioMart (Catalog No ...
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bioRxiv - Biochemistry 2023Quote: Microscale thermophoresis was performed using the Monolith Pico device (Nanotemper Technologies) as described previously (33) ...
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bioRxiv - Biochemistry 2023Quote: ... with the Prometheus device (Nanotemper Technologies). Measurements were carried out from 20°C to 95°C with a heating rate of 1°C/min.
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bioRxiv - Biochemistry 2023Quote: ... Each sample was measured with two technical and three independent biological replicates in standard capillaries (Nanotemper Technologies, Munich, Germany) with the Prometheus device (Nanotemper Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... then Plex nanoDSF Grade High Sensitivity Capillaries (NanoTemper) were filled with 10-µl protein sample ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... MST experiments were performed on a NanoTemper Monolith NT.115 (NanoTemper Technologies, Germany) with red filters at 25 °C and 20% MST power.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Recombinant METTL16 MTD (residues 1-291) protein was labelled via His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH, Germany). The labelled protein was diluted in MST buffer (10 mM Na-phosphate buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the samples were loaded into Monolith Premium Capillaries (NanoTemper Technologies GmbH, Germany). Measurements were done with Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... MST data was analyzed in NT Analysis software (NanoTemper Technologies)
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and subjected to the MST measurement ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies) were added to concentrations of 80□nM and 5□nM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The MO.Affinity Analysis software v2.3 (NanoTemper Technologies GmbH, Germany) was used for Kd value calculations ...
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bioRxiv - Molecular Biology 2023Quote: ... Each binding experiment was performed in three independent runs and resulting MST data loaded to MO.Analysis (NanoTemper) software to estimate the Kd for MRE11 dimerization ...
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bioRxiv - Plant Biology 2023Quote: ... and mixed with different concentrations of barley total RNA (Phenol/Chloroform) before loading onto Monolith NT.115 (NanoTemper Technologies). Data were treated by the KD Fit function of the Nano Temper Analysis Software (version 1.5.3).
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bioRxiv - Plant Biology 2023Quote: The fluorescent dye NT-647 (MO-L001, NanoTemper Technologies) was used to label effector proteins GST or BSA ...
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bioRxiv - Biochemistry 2023Quote: ... The measured data were fitted to the Hill equation using NT analysis software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2023Quote: ... Measurements were taken by using a Monolith NT.115 Instrument (NanoTemper Technologies) at 25 °C according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... using a His-tag Labeling Kit (NanoTemper Technologies, cat. no. MO-L018). For measurements using CBP30 ...
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bioRxiv - Genomics 2023Quote: The NanoTemper Prometheus (NanoTemper Technologies) is a nano-DSF that can determine Thermal unfolding temperatures using the intrinsic fluorescence of the protein over a temperature gradient ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The MST assay was carried out on a Monolith NT.115 (NanoTemper Technologies). CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies ...
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bioRxiv - Biophysics 2023Quote: All samples were run on a Prometheus NT.48 nanoDSF instrument (NanoTemper Technologies). Samples were set up in triplicate at a final concentration of 1 mg/mL (10 μL/capillary) ...
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bioRxiv - Biophysics 2023Quote: ... Experiments were analyzed with MO.Control and MO.Affinity analysis software (NanoTemper Technologies).
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bioRxiv - Biophysics 2023Quote: The recombinant RAD52 FL protein was labeled with the Monolith labeling kit RED-NHS (ammine dye NT-647-NHS) according to manufacturer indications (NanoTemper Technologies). Before MST experiments ...
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bioRxiv - Biophysics 2023Quote: MST measurements were performed using Monolith NT.115pico instrument (NanoTemper Technologies, Munich, Germany). Assays were conducted at 5%-10% (RED dye ...
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bioRxiv - Molecular Biology 2023Quote: ... Excitation power and MST power of Microscale Thermophoresis instrument (Monolith NT.115, NanoTemper Technologies) was set to 50% and 20% ...
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bioRxiv - Biochemistry 2023Quote: ... Data analysis was performed with NTAffinityAnalysis software (NanoTemper Technologies) in which the binding isotherms were derived from the raw fluorescence data and then fitted with both NanoTemper software and GraphPad Prism to determine the Kd using a nonlinear regression method ...
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bioRxiv - Biochemistry 2023Quote: The nanoDSF measurements were performed on a Prometheus NT.48 from NanoTemper using the corresponding software for measurement and analysis ...
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bioRxiv - Molecular Biology 2023Quote: The melting temperature Tm of a TadA protein was determined using nano differential scanning fluorimetry (nanoDSF,Nanotemper Prometheus NT.48 system) [40] ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated at room temperature for 120 min and subsequently filled into capillaries (NanoTemper Technologies) for the measurements ...
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bioRxiv - Molecular Biology 2023Quote: ... MST experiments were performed on a Monolith NT.115Pico system (NanoTemper). Purified MRE11 was fluorescently labelled with an amine-reactive AlexaFluor-647 dye ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 (NanoTemper Technologies). Spike ectodomains were diluted to approximately 0.15LJmg/mL ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Measurements were done with Monolith NT.115 instrument (NanoTemper Technologies GmbH, Germany) using red LED source ...
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bioRxiv - Bioengineering 2023Quote: ... The samples (10 µL at 0.2 mg/mL in HBS) were loaded into standard treated NT.115 capillaries (NanoTemper), and absorption at 330 and 350 nm was measured on a Prometheus NT.48 instrument (NanoTemper ...
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bioRxiv - Biochemistry 2023Quote: In order to assess protein stability of all enzymes used in this study a thermal stability assay was performed using nanoDSF (Prometheus NT.48, NanoTemper Technologies GmbH). In this assay the enzymes were diluted to final concentrations of 0.5 mg/ml (USP1/UAF1 ...