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Citations for Agilent :
2251 - 2300 of 2610 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured from the plated astrocytes using the Seahorse XFe96 Cell Metabolism Analyzer (Agilent). Astrocytes were dissociated to single cells and plated at a density of 1×104 per well ...
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bioRxiv - Neuroscience 2020Quote: ... iPS-Mg were plated and matured on Seahorse cell culture microplates and analysed using a Seahorse XFe96 Analyser (Agilent Technologies) as previously described (Piers et al ...
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bioRxiv - Genomics 2021Quote: ... and electroporated in five separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent). After a 1 hr outgrowth in Luria Broth (LB ...
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bioRxiv - Microbiology 2020Quote: ... and extracellular acidification rate (ECAR) were measured on Seahorse XF96 analyzer using Seahorse XF Cell Mito Stress Test Kit (Agilent). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant viruses were produced in 293T cells by cotransfecting pLPCX plasmids with pVPack-GP and pVPack-VSV-G packaging plasmids (Stratagene). The pVPack-VSV-G plasmid encodes the vesicular stomatitis virus (VSV ...
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bioRxiv - Cell Biology 2020Quote: ... One hour before the analysis the cells were washed twice in phenol red and bicarbonate free DMEM Seahorse Media (SM) (part nb 103575-100, Agilent) containing 25 mM glucose (Cat nb ...
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bioRxiv - Cell Biology 2020Quote: ... One hour before the analysis the cells were washed twice in SM phenol red free and bicarbonate free DMEM (part nb 103335-100, Agilent), 25 mM glucose ...
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bioRxiv - Biophysics 2021Quote: ... cells were resuspended by 200 μL 1% (w/v) BSA in PBS for detection by a NovoCyte flow cytometry (Agilent).
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bioRxiv - Cell Biology 2021Quote: ... to exclude the dead cell population and directly analyzed using flow cytometry on a Novocyte flow cytometer (Acea Biosciences, Agilent). The population of interest was gated according to its FSC/SSC criteria ...
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bioRxiv - Cell Biology 2020Quote: ... The OCR was then measured with an XF24 Extracellular Flux Analyzer via the XF Cell Mito Stress Kit (Agilent Seahorse). Following 5 measurements of basal OCR ...
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bioRxiv - Immunology 2021Quote: ... 3×105 CD8+ T cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose and 2 mM glutamine ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated in 100 ng/mL DAPI for 10 minutes before mounting slides using the Fluorescent mounting medium (DAKO). Cells were visualized on Zeiss LSM780 confocal microscope using the ZEN software ...
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bioRxiv - Biochemistry 2022Quote: ... These L6 myoblasts were seeded 24 h before experiments at 30,000 cells per well on either XF24 tissue culture plates (Agilent-Seahorse) for extracellular flux analysis ...
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bioRxiv - Biochemistry 2022Quote: Oxygen consumption rate (OCR) and extracellular acidification (ECAR) were measured using the Seahorse XF Cell Energy Phenotype Test Kit (Agilent). 12 × 103 cells per well were reverse transfected with siRNA control or targeting SORD in a Seahorse XF24 Cell Culture Microplate (Agilent ...
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bioRxiv - Immunology 2022Quote: ... approximate ~1 ×105 TH17 cells were suspended in 50 μL assay medium (Seahorse XF RPMI Assay Medium, pH 7.4, Agilent Technologies) containing 10 mM glucose ...
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bioRxiv - Plant Biology 2022Quote: His6-MBP-PFA-DSP protein fusions or free His8-MBP were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells (Stratagene). Overnight bacterial cultures were inoculated 1:1000 into fresh 2YT medium (1.6 % tryptone ...
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bioRxiv - Pathology 2022Quote: ... Anti-CD138 antibody was used for detection of plasma cells by immunohistochemistry (MI15 clone; Agilent Dako, Santa Clara, California, USA) at 1:25-1:50 dilution range.
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bioRxiv - Genetics 2022Quote: Total RNA of each cell line was extracted 48 h after seeding and quality was assessed on electrophoretic spectra from a Fragment Analyzer (Agilent), analyzed with the PROSize software (v ...
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 cell lines ectopically expressing HyPer2-DAO-NES or HyPer2-DAO-NLS were generated by viral transduction of cells using an rAAV gene targeting system (Stratagene) and subsequent isolation of single Hyper2-fluorescent clones by cell sorting on a BD FACSAria III (BD Biosciences) ...
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bioRxiv - Developmental Biology 2022Quote: ... The pETM11-Sumo3-Tn5E54K,L372P plasmid was obtained from EMBL-Heidelberg and transformed into Escherichia coli BL21 (DE3) Gold cells (Agilent). Bacterial cultures were grown at 30◦C to a density of OD600=0.6-0.8 and protein expression was induced with 0.5 mM isopropyl-β-D-thiogalactoside (IPTG) ...
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bioRxiv - Neuroscience 2021Quote: ... RNA from cultured and transfected DRG cells was purified using the Absolutely RNA Nanoprep Kit (Agilent Tech., Santa Clara, CA). RNA quantity was measured using a NanoDrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... fixed paraffin-embedded and analysis of alpha and beta cell proliferation was performed in sections immunolabeled with anti-insulin (A5064, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: Microarray was performed on in vitro cultures of dMMR and CIN MC38 CRC cells using the SurePrint G3 Mouse Gene Expression v2 Microarray (8×60K) (Agilent) system ...
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bioRxiv - Immunology 2021Quote: ... the utility plate without cartridge was unloaded and the cell plate was loaded into the XF24 analyser (Seahorse, Agilent Technologies). After equilibration ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS and incubated with a fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200). Cell nuclei were stained with 4’ ...
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bioRxiv - Plant Biology 2022Quote: ... The concentration of copper in processed yeast cells was analyzed by inductively coupled plasma mass spectroscopy (ICP-MS; Agilent 7500).
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bioRxiv - Immunology 2019Quote: ... maximal and reserve capacities and non-mitochondrial respiration were assessed using the Seahorse XFp Cell Mito Stress Test Kit (Agilent).
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bioRxiv - Immunology 2019Quote: ... 5×105 CD8+ T-cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... the cell media was replaced with the assay media and ran using the Seahorse XF Cell Mito Stress Test and ATP Rate Assay on a Seahorse XFe96/XF96 analyzer (Agilent). Hoechst 33342 staining (ThermoFisher ...
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bioRxiv - Physiology 2019Quote: The oxygen consumption rate (OCR) of HL-1 cardiomyocytes was measured using the XFp Cell Mito Stress Test by Agilent Seahorse24 ...
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bioRxiv - Physiology 2021Quote: ... cells were subjected to a Seahorse XF Mito Stress Test using the manufacturer’s instructions for timing (Agilent, Santa Clara, CA), as described (Lassiter et al. ...
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bioRxiv - Systems Biology 2020Quote: ... lymphatic tissue was cut into 1-mm pieces with a sterile scalpel and disaggregated mechanically into a single-cell suspension using the Medimachine (DAKO) equipped with sterile cartridges holding rotating knives (Medicons ...
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bioRxiv - Microbiology 2021Quote: All viral stocks were generated in Vero cells and tested negative for Mycoplasma contamination using a MycoSensor PCR Assay Kit (Agilent). Detailed passage histories of the ZIKV isolates used in this study were previously described (56) ...
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bioRxiv - Microbiology 2019Quote: ... the circular glass cover-slips containing the cell monolayers were mounted on glass slides using an anti-fade mounting solution (DakoCytomation) and visualized using an epifluorescence microscope (Olympus BX60) ...
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bioRxiv - Biochemistry 2021Quote: ... All reactions were carried out using a TGradient (Biometra) thermocycler and resulting mutant plasmids transformed into electro competent BL21-Gold (DE3) cells (Agilent). The integrity of all constructs and PK mutations were confirmed by sequencing (Genewiz).
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bioRxiv - Biochemistry 2021Quote: ... a 15-cm dish of cells was washed with PBS and UV-irradiated (120mJ/cm² at 254 nm) using a UV Stratalinker 2400 (Stratagene). Next ...
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bioRxiv - Cell Biology 2020Quote: ... including glutathione S-transferase (GST)-NEPH1 cytoplasmic domain (CD) and GST-NEPHRIN CD were expressed and purified from Escherichia coli BL21 cells (Stratagene). Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene) ...
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bioRxiv - Neuroscience 2021Quote: Flow cytometry experiments were performed on at least 200,000 cells for each sample by using Novocyte 3000 (Agilent Technologies, Inc) equipped with 488 nm lasers ...
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bioRxiv - Biochemistry 2021Quote: ... The product of each mutagenesis reaction was transformed into XL1-Blue Electroporation-Competent Cells (Agilent Technologies, Santa Clara, CA, USA) and serial dilutions were plated to determine the total number of colonies ...
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bioRxiv - Genomics 2020Quote: BG4-encoding plasmid (kindly provided by Professor Shankar Balasubramanian, University of Cambridge, UK) was transformed into BL21(DE3) competent cells (Stratagene) which were cultured in TY medium (1.6% tryptone peptone ...
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bioRxiv - Cell Biology 2021Quote: The oxygen consumption rate (OCR) in MIN6 cells were monitored by an Extracellular Flux Analyzer (XFe96, Agilent Tchnologies, CA, USA). 3×104 cells (wildtype and TMEM24 KO ...
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bioRxiv - Genomics 2021Quote: ... The cfDNA concentration was determined using an Agilent 4200 TapeStation System with the Cell-free DNA ScreenTape Analysis assay (Agilent).
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bioRxiv - Cell Biology 2021Quote: ... Total RNA from cells was quantified by nanodrop and total RNA from exosomes was quantified using an RNA bioanalyzer (Agilent). Typically ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed thrice in PBS and incubated for 30 min with HRP-conjugated rabbit anti-mouse antibody (DaKo, P0161) diluted 1:5000 in blocking buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and spare respirometry capacity were calculated using the Seahorse XF Cell Mito Stress Test Report Generator via Wave Software (Agilent) normalized to total protein levels.
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bioRxiv - Cell Biology 2022Quote: Optimal seeding density and FCCP concentration without toxicity response were tested before starting the Seahorse XF Cell Mito Stress Test assay (Agilent). A cell density of 4×103 cells per well and a FCCP concentration of 2 µM was determined and used for the following assays ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from cells was quantified by nanodrop and total RNA from exosomes was quantified using an RNA bioanalyzer (Agilent). Typically ...
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bioRxiv - Genomics 2022Quote: ... The cfDNA concentration was determined using an Agilent 4200 TapeStation System with the Cell-free DNA ScreenTape Analysis assay (Agilent).
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bioRxiv - Immunology 2022Quote: All the tumor cells used in the paper tested negative for mycoplasma (MycoAlert®, Lonza and MycoSensor qPCR®, Agilent). The metastatic melanoma cells were cultured in RPMI 1640 (Corning ...
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bioRxiv - Immunology 2022Quote: ... and probed with 8ng/mL anti-calnexin antibody (Cat. No. 2433S, Cell Signalling) overnight followed by 25ng/mL goat anti-rabbit -HRP (Cat. No. P0448, Agilent) secondary antibody or with 600ng/mL anti-GAPDH directly conjugated with HRP (Cat ...