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Citations for Agilent :
2051 - 2100 of 2440 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice and changed into Seahorse XF DMEM or RPMI medium (Agilent Technologies, 103680-100, 103681-100) containing 10 mM glucose ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed and incubated for 60 min in a CO2-free incubator in seahorse assay base media (Agilent) containing 25mM glucose (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... NEBuilder HiFi DNA Assembly Master Mix was used to assemble the inserts and backbones according to the manufacturer’s protocol and transformed into XL10-Gold competent cells (Agilent). Bacteria containing the constructs were then grown overnight at 37°C and the DNA was extracted using the Zyppy Plasmid Miniprep Kit (Zymo Research ...
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bioRxiv - Cancer Biology 2023Quote: ... Thermo Fisher Scientific)-coated XF96 plates at 0.12×106 cells/well in 180 μL of XF base medium minimal DMEM media (#102353-100, Agilent) supplemented with 20 mM D-glucose (G6152 ...
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bioRxiv - Biochemistry 2023Quote: ... GST and GST-fused CBS-pair domain of murine CNNM2 were expressed in transformed BL21 (DE3) cells (Stratagene, CA). Bacterial cells were lysed by sonication in ice-cold PBS containing 1% Triton X-100 and protease inhibitor phenylmethylsulfonyl fluoride (Sigma–Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... 293FT cells were grown to ∼90% confluency in Corning Hyperflasks and co-transfected with 129 μg of pHELPER (Agilent), 238 μg of Rep-Cap plasmid encoding MG1.2 ...
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bioRxiv - Pathology 2023Quote: Mitochondrial bioenergetics was determined by measuring oxygen consumption rate (OCR) of RPE cells with a Seahorse XFe96 Analyzer (Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2023Quote: The mitochondrial function of MAFs was measured with a Seahorse XF Cell Mito Stress Test Kit (103015-100, Agilent) using a Seahorse XF96 Extracellular Flux Analyzer ...
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bioRxiv - Cell Biology 2023Quote: HAECs were seeded at a density of 5,000 cells/well per on Seahorse XF24 tissue culture plates (Seahorse Bioscience). On the day of experiment ...
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bioRxiv - Physiology 2023Quote: ... Secreted protein in cell culture media was enriched using StrataClean Resin (Agilent, 10 µl per 5 ml of media) as previously described11,24.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Raw 264.7 cells and BMDMs were seeded in Seahorse XF2 96 culture plates (102416-100, Agilent, Craven Arms, UK) 24 hours prior to treatment ...
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bioRxiv - Biophysics 2023Quote: ... The PCR product was digested overnight with DpnI at 37 °C and transformed into XL1-Blue Supercompetent cells (Agilent) and the mutation was confirmed by sequencing (Eurofins Genomics) ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture media was replaced with assay media (Seahorse XF DMEM medium pH 7.4, Cat. No. 103575-100, Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: T cell oxygen consumption rate (OCR) was measured using a Seahorse XFe96 Extracellular Flux Analyzed following established protocols (Agilent). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... Primary Cells were stained with DAPI and then imaged with a high throughput imaging system (BioTek Cytation 5, Agilent).
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed once with DMEM Assay Medium (XF DMEM [Agilent] supplemented with XF glucose [10 mM, Agilent] ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies in DAKO REALTM Anti-body diluent (Agilent, Cat#S2022). Secondary antibodies (1:500 dilution ...
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bioRxiv - Cell Biology 2024Quote: The oxygen consumption ratio (OCR) was determined using Seahorse XF Cell Mito Stress Test (Agilent, Santa Clara, CA, US). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Cellular OCR was measured using a Seahorse XF Cell Mito Stress Test and a Seahorse Bioanalyzer XFe96 (Agilent Technologies) according to the manufacturer’s standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture media was replaced with assay media (Seahorse XF DMEM medium pH 7.4, Cat. No. 103575-100, Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Following permeabilization the cells were washed three times with PBS then blocked with Protein Block (Aligent/Dako, X090930-2). Primary antibodies (ADAR1 (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: HAECs were seeded at a density of 5,000 cells/well per on Seahorse XF24 tissue culture plates (Seahorse Bioscience). On the day of experiment ...
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bioRxiv - Neuroscience 2024Quote: RNA samples of sorted Gsh2-lineage cells (eGFP+) from control and cKO embryos (E15.5) were submitted for quality control with the BioAnalyzer (Agilent) at the Genomics Core (KU Leuven) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed three times with PBS and mounted on glass - slides using fluorescent-DAKO mounting media (Agilent Technologies). Images of tubulin morphology and costainings of tubulin and Par3 were captured at the Sanford Burnham Prebys Cell Imaging Core ...
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bioRxiv - Cell Biology 2024Quote: ... To investigate mitochondrial respiration and energetic phenotypes a Seahorse XFp Cell Mito Stress Test kit (103010-100, Agilent Technologies) was used.
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bioRxiv - Cell Biology 2024Quote: Oxygen Consumption Rates (OCR) were measured using the Seahorse XF Cell Mito Stress Test Kit (Agilent, Cat. 103708-100) with a Seahorse XFe96 analyzer (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1.5×105 for UACC-257 and RPE) in a Seahorse XF96 Cell Culture Microplate (Agilent Seahorse XFe96 FluxPak: 102416-100) and were allowed to adhere for 24 h in a 37 ⍰C humidified incubator with 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: Mitochondrial oxygen consumption rate (OCR) was assessed using the generated cell lines in an XF96e Extracellular Flux Analyzer (Seahorse Bioscience), as described previously [63] ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with anti-tubulin (Thermo PA1-41331; dilution 1:250) and K9JA (Dako A0024; dilution 1:500) antibody ...
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bioRxiv - Cell Biology 2020Quote: ... fetal and adult CFs were seeded onto dopa-coated Seahorse XF96-well cell culture microplates (V3 PS, Agilent, 101085-004) at a density of 123k/cm2 ...
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bioRxiv - Pathology 2022Quote: ... Anti-CD138 antibody was used for detection of plasma cells by immunohistochemistry (MI15 clone; Agilent Dako, Santa Clara, California, USA) at 1:25-1:50 dilution range.
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bioRxiv - Microbiology 2022Quote: ... the cells were resuspended in 500 µl PBSF buffer (PBS+2% FBS) and analyzed using ACEA NovoCyte TM (Agilent Biosciences); non-transfected 293T cells served as a negative control.
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bioRxiv - Neuroscience 2020Quote: ... Extracellular acidification rates (ECAR) and oxygen consumption rates (OCR) were measured via the Cell Mito Stress Test (Agilent, 103015-100) with a Seahorse XFe96Analyzer (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... was fused with a six histidine tag (6xHis) and an AviTag (pET-28a-AviTag-GluN1C0) and expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). Expression of His-AviTag-GluN1(834-863 ...
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bioRxiv - Cell Biology 2022Quote: ... of hPASMC were measured using Seahorse XF96 metabolic analyzer and Seahorse XF Cell Mito Stress Test kit (103015-100, Seahorse XF Technology, Agilent), following manufacturer’s recommendations ...
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bioRxiv - Biophysics 2019Quote: Human CAMSAP1 residues 1474-1613 encompassing the CKK domain (HsCKK) were cloned into pET28a vector and expressed in BL21(DE3) cells (Stratagene). Following purification via immobilized metal-affinity chromatography (IMAC ...
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bioRxiv - Physiology 2019Quote: Oxygen consumption rates in C2C12 cells were measured with a Seahorse Flux Analyzer XF24 or XFe96 (Seahorse Bioscience, Billerica, MA). The cells were plated at ∼20,000-40,000 cells/well and then differentiated into myotubes ...
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bioRxiv - Immunology 2019Quote: ... BMDMs were seeded at 5×104 cells/well and analyzed the following day on a Seahorse XF 96well Analyzer (Agilent).
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bioRxiv - Developmental Biology 2019Quote: ... were dissociated and 106 cells were used to extract total RNA following manufacturer’s instructions of the Absolutely RNA Miniprep Kit (#400800, Agilent Technologies). cDNA was synthesised with 200 ng-1 μg of total RNA using M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA isolated from these cells was checked for integrity using the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA) and quantified using a Qubit fluorometric assay (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: The Mitochondrial Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in MDA-MB-231 cells were assessed by Agilent Seahorse XF Cell Mito Stress Test Kit and Agilent Seahorse XF Cell Energy Phenotype Test Kit (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... cells were treated at a final concentration of 10μg/ml Poly(I:C) or sonicated salmon sperm DNA (Agilent, Stockport, UK) for 72hrs ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in quadruplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in triplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... For bacterial expression of mEGFP::HCF in E.coli BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent Technologies, Santa Clara, CA, USA), the cDNA of the fusion protein was cloned in pET21a (Merck KGaA ...
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bioRxiv - Biochemistry 2021Quote: Tpm isoforms Tpm1.6 and Tpm3.1 (with and without Met-Ala-Ser at the N-terminus) were expressed in ArcticExpress(DE3) RIL cells (Agilent Technologies), grown in Terrific Broth (TB ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed three times with PBS and then mounted in Roti® Mount FluorCare Dapi mounting medium (#HP20.1, Dako). Cells were visualised on a Zeiss LSM700 confocal microscope using ZEN software ...
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bioRxiv - Cancer Biology 2021Quote: Multiparameter metabolic analysis of intact cells was performed in the Seahorse XF24 extracellular flux analyzer (Seahorse Bioscience, Billerica, MA, USA) as previously described (12) ...
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bioRxiv - Cancer Biology 2020Quote: ... mononuclear cells from BM of non-metastasized breast cancer patients were subjected to immunofluorescent staining for EpCAM (Ber-EP4-FITC, Agilent or HEA-125-PE ...
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bioRxiv - Cancer Biology 2020Quote: Simultaneous multiparameter metabolic analysis of live cells was performed in the Seahorse XF24®extracellular flux analyzer (Seahorse Bioscience, USA). ACC-489 and CCL-136 cells were seeded in XF24 V7 multi-well plates (15,000 cells per well ...