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Citations for Agilent :
2401 - 2450 of 2610 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Viral particles of AAV2 were packaged in transfected 293T cells with other two plasmids: pAAV-RC and pAAV-Helper (Agilent Genomics). After harvest ...
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bioRxiv - Bioengineering 2021Quote: ... were measured in hypoxic hCMEC/D3 monolayers (20,000 cells/well cultured for four days) in a Seahorse XF96 plate by XF analysis (XF24, Agilent Seahorse Technologies) as previously described [43] ...
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bioRxiv - Bioengineering 2020Quote: ... Pelleted cells by the centrifugation for 3 min at 300 rcf are stained with FITC-conjugated anti-lysozyme antibody (Dako, F0372) and APC-conjugated anti-CD24 antibody (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... or IRBITKO cells was subjected to PCR amplification (Herculase II Fusion DNA Polymerase and 5X Herculase II PCR Buffer and dNTP (Agilent Techologies) using primers flanking the region targeted by the gRNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1.5 μL of the mutagenesis reaction was transformed into SoloPack Gold supercompetent cells following the manufacturer’s protocol (Product Number 230350, Agilent, Wilmington, DE). After a 1 hour recovery ...
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bioRxiv - Microbiology 2021Quote: ... Cell extracts were each resuspended in 50 μl methanol and 10 μl applied to high-performance liquid chromatograph (HPLC) (Agilent Technologies). AzoSM was detected at 365 nm on a C-8 column with a flow rate of 0.5 ml/min in methanol/water 90:10 ratio buffered with 1mM ammonium formate and 0.2% formic acid (Figure S8) ...
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bioRxiv - Microbiology 2021Quote: ... using a Cary WinUV UV-visible spectrophotometer equipped with a Cary Dual Cell Peltier accessory (Agilent Technologies, Santa Clara, CA, USA). Reactions were carried out at 25 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: A pET-24B plasmid containing Marseillevirus marseillevirus doublet histones (Hδ-Hγ with a C-terminal 6×His tag and Hβ-Hα) (Valencia-Sanchez et al., 2021) was transformed into Escherichia coli BL2-codon plus(DE3)-RIL competent cells (Agilent Technologies) and cultured at 37°C in 2xYT-Kanamycin medium ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells were quantified based on the number of GFP+ objects identified per scanned area using BioTek Gen5 Imager Software (Agilent Technologies). To assess T cell activation based on cytokine release ...
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bioRxiv - Cell Biology 2022Quote: ... Specially designed polystyrene tissue culture-treated 96-well microplates with a clear flat bottom (Seahorse XF96 V3 PS Cell Culture Microplates; Agilent Technologies) were then seeded with 80 μl of cell suspension (4.4×105 cells/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... ECAR (mpH/min) and OCR (pmol/min) values were obtained using Seahorse XFp Cell Energy Phenotype Test Kit (Seahorse Bioscience Inc.). Experiments were performed in triplicate and repeated at least three times.
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bioRxiv - Neuroscience 2022Quote: ... CAS No 1582-09-8) was accessed using the Seahorse XF 96 Extracellular Flux Analyzer and the XF Cell Mito stress test kit (Agilent Technologies) following Agilent Technologies guidelines ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for mycoplasma at the beginning of the studies (tested negative with MycoSensor PCR Assay Kit, Agilent, #302109) and have never exhibited contamination symptoms after initial testing.
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bioRxiv - Cancer Biology 2022Quote: ... Plates were read at 450 nm and 560 nm wavelengths using a BioTek Cytation 3 Cell Imaging Multi-Mode Microplate Reader (Agilent Technologies). Plasma samples isolated from retroorbital bleeds were used for ProcartaPlex immunoassays (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: Mitochondrial function was evaluated to assess typical bio-energetic profile of cells by directly measuring Oxygen consumption rate (OCR) in differentiated groups using Seahorse XFP (Seahorse Bioscience, Santa Clara ...
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bioRxiv - Cell Biology 2021Quote: Primary SVF cells from BAT and sWAT were isolated and cultured for 4 days before being plated in XF cell culture microplates (Seahorse Bioscience). SVF cells (10,000 cells ...
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bioRxiv - Cell Biology 2021Quote: ... they were prepared and assayed for mitochondrial function assessment according to manufacturer instructions (Seahorse XF Cell Mito Stress Test Kit, Agilent Technologies). More details are found in Supplemental Methods.
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bioRxiv - Microbiology 2020Quote: ... ACE2 was detected using a rabbit polyclonal anti-hACE2 antibody (Cell Signalling, 4355S; citrate antigen-retrieval) and EnVision+ anti-rabbit HRP (Agilent, K4003). Slides were scanned with a bright field slide scanner (Leica ...
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bioRxiv - Cancer Biology 2020Quote: ... The PmeI-linearized pShuttle-vectors carrying different cDNA fragments were introduced into the replication-deficient adenoviral vector pAdEasy-1 through homologous recombination in BJ5183 cells (Agilent Technologies). Successfully recombined adenoviral vector was verified by sequencing ...
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bioRxiv - Biophysics 2021Quote: HIV-1 Pol constructs of the BH10 strain (gift from Michael Parniak) were cloned into a pET28a vector and transformed into BL21-CodonPlus (DE3)-RIL cells (Agilent Technologies) alone or co-transformed with MBP-IBD ...
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bioRxiv - Immunology 2022Quote: Life cell metabolic analyses were performed measuring extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) using Seahorse XFp Analyzer (Agilent Technologies). 4×105 cells per well were seeded and incubated for 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... were isolated by flow cytometric cell sorting into 96-well plates and preamplification-free single-cell whole genome sequencing libraries prepared using a Bravo automated liquid handling platform (Agilent Technologies) as previously described31,32 ...
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bioRxiv - Biophysics 2019Quote: All plasmids for expression of MBP-5-HT3A-ICD fusion constructs with wild-type or engineered ICD were transformed into Escherichia coli (E. coli) BL21-CodonPlus-(DE3)-RIPL cells (Agilent Technologies). Cells were grown in Terrific Broth (TB ...
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bioRxiv - Cancer Biology 2020Quote: Ribonucleic acid was isolated from cells in indicated experimental conditions using a Qiagen miRNAeasy kit (Valencia, CA) and measured on an Agilent Bioanalyzer (Agilent Technologies). Illumina Novaseq 6000 libraries were prepared and sequenced by Novogene (CA ...
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bioRxiv - Biochemistry 2019Quote: Oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) of muscle stem cells were measured using an XF24 Bio analyzer (Seahorse Bioscience), as described previously (Forni et al ...
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bioRxiv - Biochemistry 2019Quote: ... the gene coding for the kinse domain of the Elk receptor was amplified from TKB1 cells (Agilent Technologies, Santa Clara, CA) and cloned with a C-terminal HA-tag between NdeI and EcoRV restriction sites within the second open reading frame of the pCDF-Duet vector ...
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bioRxiv - Cell Biology 2019Quote: The intact cellular OCR of LonP1 stable knockdown and control H9c2 cells was measured using a Seahorse XF-96 Extracellular Flux Analyzer (Seahorse Bioscience) as described previously (40) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated at 37°C and the medium was replaced with 180 μl XF assay medium (#102365-100, Seahorse Bioscience) containing 10 mM glucose ...
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bioRxiv - Cell Biology 2020Quote: ... parental HCT116 cells and HCT116 cells with SMYD5 overexpression (OE) or knockout (KO) were seeded in 96-well plate (in triplicates) (#101085-004, Seahorse Bioscience) one day before measurement ...
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bioRxiv - Physiology 2020Quote: ... Rod shaped adult cardiomyocytes were collected by settling down of cells and plated in 24 well plates for XF24e Extracellular Flux Analyzer analysis (Seahorse Bioscience) or on glass coverslips for confocal imaging.
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bioRxiv - Neuroscience 2020Quote: ... the original plasmid was digested with DpnI restriction enzyme and the remaining mutagenized plasmid was transformed into XL10-Gold® ultracompetent cells (Agilent). Integrity of the insert sequence and introduction of the point mutation were confirmed by Sanger sequencing of the isolated plasmid DNA.
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bioRxiv - Synthetic Biology 2021Quote: ... The molarity of the single cell libraries was calculated based on their library sizes as measured using a bioanalyzer (Agilent Technologies) and using the KAPA qPCR quantification (KAPA ...
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bioRxiv - Neuroscience 2019Quote: ... astrocytes (5×104 cells/well) were plated in a Seahorse XFe 24 microplate (Seahorse Biosciences/Agilent Technologies, Santa Clara, CA, USA) coated with 0.05mg/ml PDL and treated with 1 μM cisplatin or vehicle for 24h ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed four times with PBS containing Ca2+ and Mg2+ and mounted in a fluorescent mounting medium (DAKO, CA, USA). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time oxygen consumption rate (OCR) of these cells was measured using the Seahorse Extracellular Flux (XFe-96) analyzer (Agilent Technologies). Protein concentration of cells in each well was determined ...
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bioRxiv - Cell Biology 2021Quote: The bioenergetic profile of BMDMs were assessed by measuring the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using a XF cell Mito Stress Test kit and XF glycolysis stress kit (Agilent Technologies) as per manufacturer’s instructions using a Seahorse XF extracellular flux analyzer (Agilent technologies ...
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bioRxiv - Physiology 2020Quote: ... and centrifuged for 5 min at 1000 rpm and the pellet was re-suspended and plated on XF96 cell culture microplates (Cat# 101085-004, Agilent Technologies) coated with poly-D-lysine (Cat# P6407 ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... Total RNA from all other cell lines was used to prepare cDNA sequencing libraries using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies) after the polyA selection step ...
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bioRxiv - Physiology 2021Quote: ... Glutathione S-transferase (GST) hERG1a N-terminal domains or GST-only negative controls constructs were grown in BL21 (DE3) competent cells (Agilent Technologies) until they reached exponential growth ...
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bioRxiv - Cancer Biology 2020Quote: ... OVCAR5 shctrl and shFZD7 cell lines were seeded in Seahorse 96-well microplate (Agilent, Cat#102416-100, Santa Clara, CA, USA) at a density of 10-80K per well.
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bioRxiv - Developmental Biology 2021Quote: PSM cells were dissociated on day 2 of differentiation and reseeded onto fibronectin-coated Seahorse plates (Agilent cat. no. 101085-004) at a density of 7 x 105 cells per cm2 in Seahorse XF DMEM (Agilent cat ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA extracted from FACS isolated cells was quantified and assessed for quality using the RNA 6000 Pico Kit (Agilent – 5067-1513) and an Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured in B cells using a Seahorse XF24 Extracellular Flux Analyzer (Agilent Technologies) with kits from Agilent (Santa Clara ...
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bioRxiv - Cancer Biology 2021Quote: ... we next prepared the sorted nuclei for pre-amplification-free mini-bulk/single-cell whole genome sequencing using a Bravo liquid handler platform (Agilent Technologies) as previously described (56) ...
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bioRxiv - Cancer Biology 2021Quote: Whole RNA was extracted from three replicates for each of the cell-lines followed by a quality assessment using Agilent Bioanalyser-2100 with Agilent nano Kit (Agilent Technologies). For each sample ...
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bioRxiv - Immunology 2022Quote: ... The assay was performed for the next 7 days and each day absorbance was read at 570 nm using Cytation 5 Cell Imaging Multi-Mode Reader (Agilent, BioTek). The assay was performed in triplicate.
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bioRxiv - Neuroscience 2022Quote: ... the sections or cell containing coverslips were mounted on gelatin coated slides with Fluorescent Mounting Medium (S3023, Dako Cytomation, Glostrup, Denmark). The following primary antibodies were used ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting mixture was digested with DpnI and 5 µL of the mutagenesis reaction was transformed in XL10-Gold® Ultracompetent Cells (Agilent) and selected on amplicillin-containing LB-agar plates ...
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bioRxiv - Bioengineering 2022Quote: ... Two-channel 54.8 μm z-stack images were taken every 3 hours for 45 hours after co-culture was initiated using a Cytation 5 cell imaging multimode reader (Agilent Technologies). Images were processed with NIH Fiji-ImageJ and cell coverage was measured for each cell type at every time point by calculating the area within the well covered by cells and dividing it by the total well area ...
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bioRxiv - Bioengineering 2022Quote: Cells were equilibrated 45 min before metabolic characterization in initial volume 150 μL Seahorse XF base medium (103334-100, Agilent Technologies) containing additional (in mmol L−1 ...