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Citations for Agilent :
2351 - 2400 of 2610 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The sensor plate was calibrated prior to loading cells and running a mitochondrial stress test using a Seahorse XFe96 (Agilent). Following assays ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell Mito Stress Test was further combined with substrate pathway-specific inhibitors (etomoxir, UK5099, BPTES) (Agilent Technologies, Santa Clara, CA) to interrogate which of the three primary substrates (long chain fatty acids ...
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bioRxiv - Biochemistry 2023Quote: ... Successful mutation of isolated clones was verified by Sanger sequencing and clones were retransformed into BL21-CodonPlus (DE3)-RP competent cells (Agilent) for protein expression.
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bioRxiv - Immunology 2023Quote: ... non-targeted BMDCs or HIF-1α-deficient BMDCs were plated in Seahorse XF96 Cell Culture Microplates (Agilent Technologies, #101085-004) and stimulated with 10 mM of L-LA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Measurements of real-time of oxygen consumption rates (OCR) were determined in whole cells using a Seahorse XF96 extracellular flux analyser (Agilent) as described previously (78) ...
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bioRxiv - Molecular Biology 2023Quote: RNA was extracted from HEK293T cells as described above and RIN determined to be 10 for all samples (Bioanalyzer 6000, Agilent). Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina ...
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bioRxiv - Plant Biology 2023Quote: PHDsuper-FN3VRN5 from Zm for XL-MS analysis was inserted into pEC-LIC-His-3C containing a hexa-histidine tag and 3C protease cleavage site at the N terminus and was expressed in BL21 CodonPlus (DE3)-RIL cells (Agilent) in LB medium ...
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bioRxiv - Developmental Biology 2023Quote: Single-cell 3′ RNA-seq and single-cell ATAC-seq libraries were quantified on an Agilent Bioanalyzer with a high-sensitivity chip (Agilent), and Kapa DNA quantification kit for Illumina platforms (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... and 5.47*105 cells/cm2 were plated in each well of a poly-D-lysine-coated Seahorse XFe24 V7 PS Cell Culture Microplate (Agilent) or a 24-well tissue culture plate ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-incubated for 1 h and treated in Agilent Seahorse XF Base Medium (Agilent Technologies, cat#103334-100), which is optimized for extracellular flux assays and contains thiamine at 8.2 μM concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Several metabolic parameters were assessed in sorted cDC1 using the XF Cell Mito Stress Test and Seahorse XFe96 Analyzer (Agilent). cDC1s were labeled with PE-XCR1 antibody for positive selection using magnet after pan-DC enrichment (Miltenyi) ...
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bioRxiv - Neuroscience 2023Quote: Double immunofluorescence for activated pro- and anti-inflammatory microglial cells was performed as above with rat anti-CD86 (M1; 1:1600 dilution, Dako) and goat anti-CD206 (M2 ...
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bioRxiv - Genomics 2023Quote: ... and mock-edited control cells (CTRL) were plated on poly-D-lysine coated 96-well Seahorse assay plates (XF96, Agilent) in DMEM (Gibco A144300 ...
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bioRxiv - Cell Biology 2023Quote: ... Vectors encoding the recombinant proteins were constructed as described in the previous section and introduced into the BL21-CondonPlus (DE3)-RIL competent cells (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The optical density was measured at 540 nm and subtracted from readings at 450 nm using the BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Concentrations were calculated from standard curves run in parallel.
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bioRxiv - Immunology 2023Quote: DNA whole exome libraries of B16-F10 cell line and C57BL/6JOlaHsd germline sample were prepared with Agilent Mouse All Exon kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... then plated at 3mL cell suspension/well into 44mm 24-deep-well round-bottom culture plates (Agilent Technologies #202061-300) using a Multidrop Combi (Thermo Fisher).
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bioRxiv - Physiology 2023Quote: ... The cells were transfected with 100 ng of NF-κB firefly luciferase reporter plasmid p(NF-κB)3-Luc (Stratagene) and 10 ng of Renilla luciferase reporter plasmid pRL-RSV (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.5 × 105 Bmal1::luciferase MAFs or Per2::luciferase PDA cells were seeded per well in 96-well black-sided imaging plates (101085-004, Agilent) pre-coated with 0.2% gelatin for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were tested for oxygen consumption rate and ATP production following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). Measurements were taken both at baseline prior to mitochondrial stress and following the addition of 2 μM FCCP uncoupling agent.
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bioRxiv - Neuroscience 2023Quote: ... the corresponding pAAV plasmid construct along with AAV helper plasmids encoding the structural elements were transfected using polyethylenimine (PEI) into the AAV-293 cell line (CVCL_6871, Agilent, USA). The day before transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA obtained from cell cultures transfected with the RNP complex was amplified by nested PCR using Herculase II Fusion DNA Polymerase (Agilent). The first amplification was performed with primers IG36 and IG37 as follows ...
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bioRxiv - Microbiology 2023Quote: ... Membrane fluidity was determined by using an excitation wavelength of 350 nm and measuring fluorescence at 460 nm and 500 nm emission wavelengths on a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The generalised polarisation (GP ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl were aliquoted into black 96 well plates in triplicate and fluorescence was measured using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The excitation wavelength used was 488 nm and emission wavelength used was 530 nm.
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bioRxiv - Cancer Biology 2023Quote: ... The organic samples were resuspended in 35x the packed cell volume using 2:1:1 isopropyl alcohol:acetonitrile:water and transferred to amber glass mass spectrometry vials (Agilent, 5182-0716) containing a glass insert ...
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bioRxiv - Cell Biology 2023Quote: ... Successful differentiation into cholangiocyte-like and hepatocyte-like cells was tested validated by immunostaining against albumin (Agilent, Cat. Nr. A0001). Primary human hepatocytes (PHHs ...
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bioRxiv - Bioengineering 2023Quote: ... All morphology and Cell Painting liquid handling (fixing, staining, washing) was performed using a BioTek MultiFlo FX automated liquid handler (Agilent).
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bioRxiv - Microbiology 2023Quote: OCR and ECAR were measured in XF96 cell culture microplates using a Seahorse XFe96 Extracellular Flux Analyzer (Seahorse, Agilent Technologies). ISE6 cells were seeded at densities of 120 – 150,000 cells per well in complete L15C300 media41 and incubated at 34°C for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... 500 or 750 μM DIP prior to transfer of 200 μL to the wells of a Seahorse XF96 V3 PS cell culture microplate (Agilent). The seahorse XF sensor cartridge (Agilent ...
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bioRxiv - Microbiology 2024Quote: ... cover slips containing cells were mounted using VITROCULD mounting media (Deltalab) and viewed using Lionheart LX Automated Microscope (Agilent BioTek) at 40X magnification ...
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bioRxiv - Biochemistry 2024Quote: ... The plates were then shaken for 2 minutes and luminescence was measured using the BioTek Cytation 5 cell imaging multimode reader (Agilent).
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bioRxiv - Cell Biology 2024Quote: ... SC/KD-miRNA-transduced MIN6 cells were cultured in 2 mM glucose and treated with a total RNA isolation mini kit (Agilent) and 1st strand synthesis kit (Origine) ...
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bioRxiv - Biophysics 2024Quote: Absorption spectra of whole cells and isolated photosystems were measured between 400 and 800 nm with a Cary 50 UV-Vis spectrophotometer (Agilent). To minimise light scattering ...
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bioRxiv - Cell Biology 2024Quote: Measurements of oxygen consumption were conducted with the Seahorse Bioscience XFe96 bioanalyzer using the Seahorse XF Cell Mito Stress Test Kit (Cat# 103015-100, Agilent). hiPSC and progenitors were seeded on XF96 cell culture microplates (Cat# 102416-100 ...
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bioRxiv - Cancer Biology 2024Quote: Mitochondrial oxygen consumption rates (OCR) were measured using the Agilent Seahorse XF Cell Mito Stress Test Kit and Seahorse XFe96 FluxPak (Agilent Technologies ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 15 worms per well in M9 buffer (200 microliter) were transferred to a Seahorse XF96 V3 PS Cell Culture Microplate (Agilent) with 10 technical replicates per treatment ...
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bioRxiv - Physiology 2024Quote: ... the cells were washed twice in phenol red and bicarbonate free DMEM Seahorse Media (SM) (part. no.: 103575-100, Agilent) containing 25 mM glucose (Cat no ...
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bioRxiv - Microbiology 2024Quote: ... viruses were titrated in Vero cells by using anti-nucleoprotein (anti-NP) mouse monoclonal antibody and horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (Dako). Monolayered Vero cells were then infected with virus at a multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2020Quote: Oxygen consumption rate (OCR) was measured during Seahorse XF Cell Mito Stress Tests as per the user guide (Agilent 103015-100) with modifications ...
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bioRxiv - Cell Biology 2020Quote: ... The cell viability was assessed by measuring the absorbance at 570 nm wavelength using Epoch 2 microplate spectrophotometer (Agilent technologies. USA).
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bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... we washed the cells twice with 1X PBS and incubated them with FITC-conjugated anti-mouse IgG (dilution 1:500; Dako Cytomation) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The PGBD1 ORF was amplified from a cDNA library prepared from HeLa cells using DNA polymerase Pfu Ultra II Fusion HS (Agilent Technologies) and PGBD1 Forw (5’-ATGTATGAAGCTTTGCCAGGC-3’ ...
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bioRxiv - Cell Biology 2022Quote: ... all cultures were diluted to seed an A600 = 0.01 cells/well into a poly-D-lysine coated Seahorse XFp cell culture miniplate in 50 μl of Seahorse XF assay media (Agilent Technologies). For background measurement ...
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bioRxiv - Biophysics 2019Quote: ... Endothelial cell phenotype was confirmed by the typical cobblestone appearance and von Willebrand Factor expression (A0082 Dako-Agilent Santa Clara CA) (Supplemental Figure 1) ...
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bioRxiv - Biophysics 2019Quote: ... Endothelial cell phenotype was confirmed by the typical cobblestone appearance and von Willebrand Factor expression (A0082 Dako-Agilent Santa Clara CA) (Supplemental Figure 1) ...
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bioRxiv - Cell Biology 2019Quote: ... and FACS-isolated eYFP positive cells (FACS ARIA IIu sorter, Becton Dickinson) were collected in Lysis Buffer (400753, Absolutely RNA Nanoprep kit, Agilent Technologies).
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bioRxiv - Developmental Biology 2019Quote: ... quality and integrity of RNA samples obtained from HUVEC cell bodies and protrusions were assessed using a 2200 TapeStation (Agilent Technologies). Next ...