Labshake search
Citations for Agilent :
2201 - 2250 of 2440 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... the corresponding pAAV plasmid construct along with AAV helper plasmids encoding the structural elements were transfected using polyethylenimine (PEI) into the AAV-293 cell line (CVCL_6871, Agilent, USA). The day before transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... then plated at 3mL cell suspension/well into 44mm 24-deep-well round-bottom culture plates (Agilent Technologies #202061-300) using a Multidrop Combi (Thermo Fisher).
-
bioRxiv - Cell Biology 2024Quote: Mitochondrial bioenergetics of MDMs and DCs were measured using the Seahorse XF Cell Mito Stress Test Kit and the XF24 Extracellular Flux Analyzer (Agilent), as previously described (100) ...
-
bioRxiv - Microbiology 2023Quote: ... Membrane fluidity was determined by using an excitation wavelength of 350 nm and measuring fluorescence at 460 nm and 500 nm emission wavelengths on a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The generalised polarisation (GP ...
-
bioRxiv - Microbiology 2023Quote: ... 200 μl were aliquoted into black 96 well plates in triplicate and fluorescence was measured using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The excitation wavelength used was 488 nm and emission wavelength used was 530 nm.
-
bioRxiv - Molecular Biology 2023Quote: ... The DNA obtained from cell cultures transfected with the RNP complex was amplified by nested PCR using Herculase II Fusion DNA Polymerase (Agilent). The first amplification was performed with primers IG36 and IG37 as follows ...
-
bioRxiv - Microbiology 2023Quote: ... 500 or 750 μM DIP prior to transfer of 200 μL to the wells of a Seahorse XF96 V3 PS cell culture microplate (Agilent). The seahorse XF sensor cartridge (Agilent ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were tested for oxygen consumption rate and ATP production following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). Measurements were taken both at baseline prior to mitochondrial stress and following the addition of 2 μM FCCP uncoupling agent.
-
bioRxiv - Systems Biology 2023Quote: Oxygen consumption rate was measured with the Seahorse XFe96 bioanalyzer using the Seahorse XF Cell Mito Stress Test Kit (Agilent), or using High-Resolution Respirometry (Oxygraph-2K ...
-
bioRxiv - Biochemistry 2023Quote: ... the cells were washed in PBS and exposed to UV (10 miliJules/cm2) in a Stratalinker UV crosslinker oven (Stratagene). 4 hours after the UV irradiation ...
-
bioRxiv - Bioengineering 2023Quote: ... All morphology and Cell Painting liquid handling (fixing, staining, washing) was performed using a BioTek MultiFlo FX automated liquid handler (Agilent).
-
bioRxiv - Cancer Biology 2023Quote: ... The organic samples were resuspended in 35x the packed cell volume using 2:1:1 isopropyl alcohol:acetonitrile:water and transferred to amber glass mass spectrometry vials (Agilent, 5182-0716) containing a glass insert ...
-
bioRxiv - Bioengineering 2024Quote: ... Two-channel 85 µm z-stack images were taken using a Cytation 5 V3.14 cell imaging multi-mode reader (Agilent Technologies). A total of 21 z-stacks per well and per experiment were recorded and used for post-processing to calculate the cell phenotypic response in each hydrogel model.
-
bioRxiv - Cell Biology 2023Quote: Oxygen consumption rate of U2OS WT and G3BP1/2 dKO cells was measured on an extracellular flux analyzer (Agilent Seahorse) using the XF Cell Mito Stress Test Kit following manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... After single-cell RNA-seq libraries were prepared and the library quality was confirmed with the TapeStation D1000 Screen Tape (Agilent) and a Qubit 3.0 dsDNA HS Assay Kit (Life Technologies) ...
-
bioRxiv - Microbiology 2023Quote: ... The optical density was measured at 540 nm and subtracted from readings at 450 nm using the BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Concentrations were calculated from standard curves run in parallel.
-
bioRxiv - Immunology 2023Quote: DNA whole exome libraries of B16-F10 cell line and C57BL/6JOlaHsd germline sample were prepared with Agilent Mouse All Exon kit (Agilent) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were stored at - 80°C until crosslinking (still frozen) with 400 mJ/cm2 UVC using a Stratalinker 2400 (Stratagene).
-
bioRxiv - Cell Biology 2023Quote: ... Successful differentiation into cholangiocyte-like and hepatocyte-like cells was tested validated by immunostaining against albumin (Agilent, Cat. Nr. A0001). Primary human hepatocytes (PHHs ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Pu.1 (1:200; 2258, Cell Signalling), hamster anti-CD3 (1:500; MCA2690, AbD Serotec) or rabbit anti-GFAP (1:2400, Z0334, Dako) diluted in the blocking solution overnight at 4°C plus one additional hour at room temperature (RT) ...
-
bioRxiv - Molecular Biology 2024Quote: Data analysis for cell culture EVs was done using the Spectrum Mill MS Proteomics Workbench software package v 4.2 beta (Agilent Technologies). All extracted spectra were searched against a UniProt database containing human reference proteome sequences ...
-
bioRxiv - Physiology 2024Quote: ... MLO-Y4 cells with Pparγ silenced with siRNA (γY4KD) and their scrambled control (Scrl) were plated 6 hrs before the assay onto Seahorse cell culture microplates (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: Primary mouse AT2s were isolated and plated as described above with measurement of mitochondrial respiration performed using the Seahorse XF Cell Mito Stress Test Kit (Agilent) or the Seahorse XF Palmitate Oxidation Stress Test Kit (Agilent ...
-
bioRxiv - Biochemistry 2023Quote: ... of C2C12 cells expressing UCP1 under the control of the doxycycline-inducible promoter (TRE3G) were measured with Seahorse XFe24 analyzer (Agilent). Cells were seeded at 4 x 104 cells per well in XFe24 cell culture microplates (Agilent ...
-
bioRxiv - Cell Biology 2023Quote: ... medium was removed and cells were washed according to manufacturer’s protocol with Seahorse assay medium consisting of DMEM (103575-100, Agilent, USA) with 10 mM glucose ...
-
bioRxiv - Biophysics 2024Quote: ... using a 3 × 3 mm black walls quartz cell at 25 °C on an Agilent Cary Eclipse spectrofluorometer (Agilent Technologies) equipped with a thermostatted cell holder attached to an Agilent PCB 1500 water Peltier system ...
-
bioRxiv - Bioengineering 2024Quote: The OCR and ECAR of hMSCs was measured using the Seahorse Cell Mito Stress Test with the extracellular flux Seahorse XFp analyzer (Agilent), in order to assess the mitochondrial respiration and glycolysis ...
-
bioRxiv - Cell Biology 2024Quote: ... Concentration and insert size distribution for both the gene expression library and the cell multiplexing library were determined with a BioAnalyzer High Sensitivity DNA Assay (Agilent). Sequencing was performed on a NovaSeq 6000 on multiple flowcells with a paired-end 150 bp configuration ...
-
bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
-
bioRxiv - Cancer Biology 2024Quote: Cellular oxygen consumption rate (OCR) was measured using a Seahorse XF Cell Mito Stress Test in a XFe24 Bioanalyzer (Agilent). Cells were plated at a concentration of 30,000 (HF2303 ...
-
bioRxiv - Cell Biology 2024Quote: ... Mitochondrial energetics was measured using a Seahorse XFe96 extracellular flux analyzer and the XF Cell Mito Stress kit (Seahorse Bioscience) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... data was exported to a Seahorse XF Cell Mito Stress Test Report Generator using the Seahorse Wave Desktop Software (Agilent). Hoechst fluorescent intensity was used to normalize for cell number.
-
bioRxiv - Cell Biology 2024Quote: ... The resulting vectors were subjected to sequencing to ensure that no mutations were introduced into the WRN sequence and were used for transforming BL21 cells (Stratagene). Expression of GST and GST-fusion proteins were induced upon addition of 1 mM isopropyl-D-thiogalactopyranoside (IPTG ...
-
bioRxiv - Biochemistry 2024Quote: ... the cells were washed in 1X PBS and exposed to UV (10 J/m2) in a Stratalinker UV crosslinker oven (Stratagene).After UV exposure ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were analyzed by NovoCyte 3000 flow cytometer equipped with three lasers and 13 fluorescence detectors (Agilent, Santa Clara, CA). GFP and Alexa Flour 647 fluorophores were excited by the 488 and 640 nm lasers ...
-
bioRxiv - Cell Biology 2020Quote: Oxygen consumption rate (OCR) was measured during Seahorse XF Cell Mito Stress Tests as per the user guide (Agilent 103015-100) with modifications ...
-
bioRxiv - Cell Biology 2020Quote: ... The cell viability was assessed by measuring the absorbance at 570 nm wavelength using Epoch 2 microplate spectrophotometer (Agilent technologies. USA).
-
bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... we washed the cells twice with 1X PBS and incubated them with FITC-conjugated anti-mouse IgG (dilution 1:500; Dako Cytomation) for 1 hour ...
-
bioRxiv - Molecular Biology 2021Quote: ... The PGBD1 ORF was amplified from a cDNA library prepared from HeLa cells using DNA polymerase Pfu Ultra II Fusion HS (Agilent Technologies) and PGBD1 Forw (5’-ATGTATGAAGCTTTGCCAGGC-3’ ...
-
bioRxiv - Cell Biology 2022Quote: ... all cultures were diluted to seed an A600 = 0.01 cells/well into a poly-D-lysine coated Seahorse XFp cell culture miniplate in 50 μl of Seahorse XF assay media (Agilent Technologies). For background measurement ...
-
bioRxiv - Biophysics 2019Quote: ... Endothelial cell phenotype was confirmed by the typical cobblestone appearance and von Willebrand Factor expression (A0082 Dako-Agilent Santa Clara CA) (Supplemental Figure 1) ...
-
bioRxiv - Biophysics 2019Quote: ... Endothelial cell phenotype was confirmed by the typical cobblestone appearance and von Willebrand Factor expression (A0082 Dako-Agilent Santa Clara CA) (Supplemental Figure 1) ...
-
bioRxiv - Cell Biology 2019Quote: ... and FACS-isolated eYFP positive cells (FACS ARIA IIu sorter, Becton Dickinson) were collected in Lysis Buffer (400753, Absolutely RNA Nanoprep kit, Agilent Technologies).
-
bioRxiv - Developmental Biology 2019Quote: ... quality and integrity of RNA samples obtained from HUVEC cell bodies and protrusions were assessed using a 2200 TapeStation (Agilent Technologies). Next ...
-
bioRxiv - Neuroscience 2020Quote: Viral particles of AAV2 were packaged in transfected 293T cells with other two plasmids: pAAV-RC and pAAV-Helper (Agilent Genomics). After harvest ...
-
bioRxiv - Bioengineering 2021Quote: ... were measured in hypoxic hCMEC/D3 monolayers (20,000 cells/well cultured for four days) in a Seahorse XF96 plate by XF analysis (XF24, Agilent Seahorse Technologies) as previously described [43] ...
-
bioRxiv - Bioengineering 2020Quote: ... Pelleted cells by the centrifugation for 3 min at 300 rcf are stained with FITC-conjugated anti-lysozyme antibody (Dako, F0372) and APC-conjugated anti-CD24 antibody (Biolegend ...
-
bioRxiv - Cell Biology 2021Quote: ... or IRBITKO cells was subjected to PCR amplification (Herculase II Fusion DNA Polymerase and 5X Herculase II PCR Buffer and dNTP (Agilent Techologies) using primers flanking the region targeted by the gRNA ...