Labshake search
Citations for Agilent :
1401 - 1450 of 1560 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Peaks were identified based on known glycans from the Glyko InstantAB biantennary and high-mannose partitioned library standards (Prozyme, Hayward, CA, USA), with the total percent of each glycoform reported as the integrated area under the identified peak divided by the sum of the AUC (area under the curve ...
-
bioRxiv - Cancer Biology 2022Quote: Extracellular acidification rate (measured in mpH/min) was monitored using an XFe24 extracellular flux analyzer from Seahorse Bioscience (Agilent, Santa Clara, CA) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... cGAMP was purified from the reaction mixture using a PLRP-S polymeric reversed phase preparatory column (100 Å, 8 μm, 300 × 25 mm; Agilent Technologies) on a preparatory HPLC (1260 Infinity LC system ...
-
bioRxiv - Bioengineering 2021Quote: ... pCS2-GemininΔ27-GFP was generated by deleting amino acids 1-27 from Geminin-GFP [27] using QuikChange® Lightning mutagenesis kit (Agilent, 210513). The plasmids pCS2-Flag-FA-Geminin-GFP w.t and Δ27 variant were generated by cloning Geminin-GFP into pCS2-Flag-FA vector sing FseI and AscI restriction sites ...
-
bioRxiv - Genomics 2021Quote: ... Cy3-labeled cRNA was generated from 200 ng input total RNA using Agilent’s Low Input Quick Amp Labeling Kit (Agilent Technologies, Tokyo, Japan). cDNA was generated with the primer containing a T7 polymerase promoter ...
-
Differences in orthodenticle expression promote ommatidial size variation between Drosophila speciesbioRxiv - Evolutionary Biology 2021Quote: ... The size range of final cDNA libraries was determined by applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). cDNA libraries were amplified and sequenced using cBot and HiSeq 2000 (Illumina) ...
-
bioRxiv - Cancer Biology 2020Quote: ... to extract total RNA cells from the various experimental conditions and tested for RNA quality using a Bioanalyzer (Agilent, Santa Clara, CA). Contaminating genomic DNA was removed using Turbo DNA free from Ambion ...
-
bioRxiv - Bioengineering 2021Quote: ... mRNA was isolated from high quality total RNA samples with RIN > 9 and 28S:18S > 1 (measured with an Agilent 2100 Bioanalyzer) using poly-T oligonucleotide beads ...
-
bioRxiv - Cell Biology 2021Quote: ... Libraries were then analyzed by qPCR generating CT values using NEBNEXT Library Quant Kit (#E7630) and by tape station with D1000 screen tape from Agilent (#5067-5582) to calculate fragment base pair size ...
-
bioRxiv - Plant Biology 2020Quote: ... Lock mass calibration was performed for the profiling of the stable lines using signal m/z 622.0289 (molecular formula C12H19F12N3O6P3) from the ESI Tuning Mix (Agilent Technologies, http://www.agilent.com). MS/MS experiments were performed using AutoMS/MS runs at various CID voltages from 12.5 to 22.5 eV for ammonium adducts ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... a 2uL aliquot from each plated sample was diluted 1:9 on a Bravo liquid handling platform (Agilent, Santa Clara, CA, USA). A 5 μL aliquot from each diluted sample was arrayed into a 384 well plate for input into the mNGS library prep ...
-
bioRxiv - Microbiology 2021Quote: ... Sections labelled for F4 were incubated with secondary antibody from kit polymer-HRP anti-rabbit (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark) while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories ...
-
bioRxiv - Cell Biology 2020Quote: His-tagged Ran in the pET28 vector (a gift from Dr. Günter Blobel) was transformed into BL21 (DE3) RIL-competent cells (Stratagene, St. Louis, MO). Expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
-
bioRxiv - Cancer Biology 2022Quote: The cellular oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) data were obtained using a Seahorse™ XF96 Flux Analyzer from Seahorse Bioscience (Agilent Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... The quality of RNA from each stage was analyzed using Agilent Bioanalyzer’s RNA 600 Nano assay class (Agilent Tech., Sta. Clara, CA) before pooling equal amounts to be used for cDNA synthesis ...
-
bioRxiv - Cancer Biology 2022Quote: ... by polymerase chain reaction (PCR). Ki-67 (Catalog no. M7240) and CD4 (Catalog no. M731029-2) antibodies were purchased from Dako (CA, USA). FOXO3a (Catalog no ...
-
bioRxiv - Microbiology 2021Quote: ... depleted serum were eluted from a high-capacity nano-HPLC Chip (160 nL, 150 mm × 75 μm ID, Agilent, Santa Clara, CA) with a 30 min gradient of 3-40% acetonitrile as described previously 28,29 ...
-
bioRxiv - Cell Biology 2020Quote: ... DMPI’s library is built upon the Fiehn GC/MS Metabolomics RTL Library (a gift from Agilent, their part number G1676-90000; (92)) ...
-
bioRxiv - Cell Biology 2020Quote: ... All STIM1 variants were generated from the wild-type (WT) construct by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene, Massy, France). All resulting plasmids were checked by Sanger sequencing.
-
bioRxiv - Developmental Biology 2020Quote: ... one μg of total RNA from LV was used to synthetize cDNAs using oligo(dT) primers and affinity script reverse transcriptase (Agilent technologies France). Real-time quantitative PCR analyses were performed using the Light Cycler LC 1.5 (Roche ...
-
bioRxiv - Cell Biology 2020Quote: ... coli-optimized synthetic gene encoding the E2 ORF from HPV-16 (GenBank: AAD33255.1) was cloned into pCAL-N-FLAG (Agilent Technologies, CA, USA), which contains a vector-encoded N-terminal calmodulin bind site (CBS) ...
-
bioRxiv - Immunology 2022Quote: ... The XF Mito Stress Test report generator and Agilent Seahorse analytics were used to calculate parameters from Wave software (Agilent, Version 2.6.1.53).
-
bioRxiv - Genetics 2022Quote: ... a sample from each library was used to assess library fragment size distribution by electrophoresis using BioAnalyzer High Sensitivity DNA Assay (Agilent Technologies, CA) and to assess library concentration by qPCR using KAPA library quantification kit (KAPA Biosystems ...
-
bioRxiv - Genomics 2022Quote: ... we indexed the blank controls with the maximum number of cycles from our samples even when there were non-detectable DNA quantities on a TapeStation instrument (Agilent Technologies, Germany).
-
bioRxiv - Neuroscience 2022Quote: ... were used for each replicate and placed into 100 μL of lysis buffer + β-mercaptoethanol from the Absolutely RNA Microprep kit (Agilent Technologies). Each genotype was prepared in quadruplicate ...
-
bioRxiv - Neuroscience 2022Quote: ... as previously described46 and alignment of sequence reads and variant calling from NGS were assessed by SureCall software (Agilent, Santa Clara, CA). Identified mutations and variants of interest in GBA1 and LRRK2 were confirmed by Sanger sequencing or TaqMan assay (ThermoFisher Scientific).
-
bioRxiv - Developmental Biology 2022Quote: ... Mutation of the highly cross-species conserved miR-483-3p seed target sequence from AGGAGUG to AGGAACG (mouse) was performed using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies – 200518). The HEK-293 cells were used for miR-483-3p expression studies ...
-
bioRxiv - Molecular Biology 2024Quote: ... sgRNA-encoding genomic loci were amplified from up to 400 µg gDNA via PCR using a Herculase II Fusion Polymerase PCR kit (Agilent, Cat. #600677). Pooled reaction products from each treatment group were uniquely barcoded in a subsequent PCR reaction followed by electrophoresis on 2% TBE-agarose gel at 120 V ...
-
bioRxiv - Neuroscience 2024Quote: ... 400 mW) modulated by a 10 kHz square wave with varying duty cycles output from a function generator (Agilent, 33220A, 20 MHz) and gated by the specific positions of the arena pattern such that either the higher bar quadrant or the lower bar quadrant was accompanied by the laser punishment aimed at the back of the fly ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... ten respective individuals of workers and alate queens from each of the ten colonies were pooled and extracted in a 1.5 ml glass vial (Agilent Technologies, Waldbronn, Germany) with 100 μl of MS pure n-hexane (Merck KGaA ...
-
bioRxiv - Genetics 2023Quote: Exome enrichment was applied to the cDNA purified from the previous step by using probe kit SSELXT Human All Exon V8 (Agilent, 5191-6879) for human samples ...
-
bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared according to the SureSelect XT RNA Target Enrichment for Illumina Multiplexed Sequencing protocol from Agilent (Ref: 5190-4393). Libraries were analyzed using a BioAnalyzer and were quantified using qPCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... the A3 sequence specifying amino acids 21-393 was amplified from pHD1344tub(PAC)-Nterm3V5-A3 by error prone PCR using the GeneMorphII Random Mutagenesis kit (Agilent, Cat # 200523) and primers that add AttB sites (Table S2 ...
-
bioRxiv - Immunology 2023Quote: ... The XF Mito Stress Test report generator and Agilent Seahorse analytics were used to calculate parameters from Wave software (Agilent, Version 2.6.1.53).
-
bioRxiv - Plant Biology 2023Quote: The aqueous fractions recovered from the fractionation described under 2.3.2 were analysed with an ICP-OES 5110 (Agilent; Santa Clara, CA, USA) for 20 elements ...
-
bioRxiv - Cancer Biology 2023Quote: The rates of ATP generation from glycolysis and mitochondria were measured via the Agilent Seahorse XFe96 Analyzer (Agilent Technologies, Santa Clara, CA) as described 80 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The dried labelled peptide plexed sample was then resuspended in 400uL of 1 M TEAB and fractionated using an Agilent 1100 HPLC system and high pH reversed phase chromatography on a Zorbax Eclipse XDB-C18 column (4.6 x 150mm, 5-micron) from Agilent (Santa Clara, CA). Sixty fractions were collected at 0.5 mL/min over 105 min using a gradient of 0-5% solvent B in 10 min ...
-
bioRxiv - Cell Biology 2023Quote: For confocal laser scanning microscopy all images were taken from fixed specimens embedded in Dako fluorescent mounting medium (Dako Inc, Carpinteria, USA) on a LEICA SP5 confocal microscope equipped with a 63.0x/1.40 NA oil HCX PL APO CS UV objective and analyzed using LAS AF Lite software ...
-
bioRxiv - Immunology 2023Quote: ... Whole-exome sequencing (WES) libraries were prepared using the SureSelect Human All Exon V6 kit from Agilent (Agilent Technologies, Santa Clara (CA), U.S.A. ...
-
bioRxiv - Cell Biology 2023Quote: Analyte areas were obtained from integrating chromatograms that corresponded to a lipid of interest using the Masshunter Quantitative analysis software (Agilent; version B9.00). Lipid concentrations were determined using the following formula:
-
bioRxiv - Neuroscience 2023Quote: ... were used for each replicate and placed into 100 µL of lysis buffer + β-mercaptoethanol from the Absolutely RNA Microprep kit (Agilent Technologies). Each genotype was prepared in quadruplicate ...
-
bioRxiv - Cancer Biology 2023Quote: Mitochondrial DNA copy number was quantified by qPCR on total DNA from gastrocnemius muscle using MxPro Mx3000P real-time PCR Analyzer (Agilent technologies / Stratagene) and determining the mtDNA/nDNA ratio ...
-
bioRxiv - Genetics 2023Quote: ... 15 PCR-2 reactions were completed using Oligo 45 paired with Oligos 47-61 to amplify from YCp50-WT_PKR plasmid using Herculase II polymerase (Agilent Technologies Cat#600677) using cycling conditions for vector targets >10 kilo-base pairs ...
-
bioRxiv - Genomics 2023Quote: ... The eluted sized RNA was separated from the gel slices by short centrifugation using cellulose acetate spin filters (Agilent cat# 5185-5990). The 5’-triphosphates on these RNAs were converted to monophosphates by treating the short nuclear RNA 5’ Polyphosphatase ...
-
bioRxiv - Cell Biology 2023Quote: ... coli cells were transformed with the plasmid for GST-SSH1 (869–1049) and then infected with a λ phage cDNA library from neonatal mouse brain (λ Uni-ZAP XR, Stratagene). ExAssist interference-resistant helper phages were coinfected with λ phage library to induce in vivo excision of cDNA into pBluescript phagemid in E ...
-
bioRxiv - Bioengineering 2023Quote: ... We drive each transducer with an AC signal from wave-form generators: two single output waveform generators (Agilent 33220A, RS components, Austria) to drive side-transducers S1 and S2 ...
-
bioRxiv - Neuroscience 2023Quote: ... the four 130 bp α-unit DNA fragments were amplified from each α-unit plasmid using the Herculase II Fusion DNA polymerase (Agilent) and oJS2581 and oJS2582 primers77 ...
-
bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from the beads and purified according to the manufacturer’s instructions using an Absolutely RNA nanoprep kit (Stratagene, La Jolla, CA) with in column DNA digestion and frozen prior to gene expression profiling being performed ...
-
bioRxiv - Immunology 2024Quote: ... PBMC from control SIV naïve and SIV-infected+ART treated macaques were plated into XF24 polystyrene cell culture plates (Seahorse Bioscience, North Billerica) at 20,000/well ...
-
bioRxiv - Cell Biology 2024Quote: ... and HP-0921 from Agilent (m/z 922.0098) in positive mode and 4-NBA (m/z 166.0146) and HP-0921 from Agilent (m/z 966.0007, formate adduct) in negative mode.