Labshake search
Citations for Agilent :
1101 - 1150 of 1560 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
-
bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... and retrovirus expressing EGFP in alternating one-in ten sections using different anti-BrdU antibodies from different vendors (for BrdU: mouse primary at 1/100, Dako; CldU ...
-
bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were as follows: polyclonal rabbit anti-goat Immunoglobulins/HRP (P0160) and polyclonal swine anti-rabbit Immunoglobulins/HRP (P0217) were from Dako, DK ...
-
bioRxiv - Bioengineering 2021Quote: ... RNA integrity was assessed using an RNA Nano 6000 Assay Kit from the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The RNA-seq library was then generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB ...
-
bioRxiv - Developmental Biology 2021Quote: ... The catalytically dead mutants MMP28EA-pCS2 and MMP28EA-ΔSP/NLS-GFP-pCS2 were produced by point mutation of Glutamic acid226 to Alanine from MMP28wtpCS2 and Glutamic acid209 to Alanine from MMP28ΔSP/NLS-GFP-pCS2 using QuikChange II site-Directed Mutagenesis Kit (Agilent #200523) with the primers MMP28_E-A_fdw ...
-
bioRxiv - Biochemistry 2020Quote: ... The gene coding for the H349Y GlgE variant was generated from the synthesized wild-type glgE gene using a QuikChange Lightening kit (Agilent). The constructs were ligated into pET21a expression vectors (Novagen ...
-
bioRxiv - Cell Biology 2021Quote: PCR amplification of inserted sgRNAs from the genomic DNA was done with the Herculase II Fusion DNA Polymerase (Agilent Technologies) using the primers oMCB-1562 and oMCB-1563 ...
-
bioRxiv - Cancer Biology 2020Quote: ... mononuclear cells from BM of non-metastasized breast cancer patients were subjected to immunofluorescent staining for EpCAM (Ber-EP4-FITC, Agilent or HEA-125-PE ...
-
bioRxiv - Microbiology 2020Quote: The PB-S-G614-ΔC-HA vector is obtained from the PB-S-D614-ΔC-HA vector using the Quickchange XL Site-directed Mutagenesis (Agilent) and the primers CoV-35 and CoV-36 ...
-
bioRxiv - Microbiology 2020Quote: ... and pCMVTag 4 expression control plasmid containing the firefly luciferase gene fused with the FLAG tag (luc-FLAG) were obtained from Stratagene. The plasmid 15cxCAT contained the interleukin-2 (IL-2 ...
-
bioRxiv - Plant Biology 2020Quote: ... desorption of the VOCs from the fiber coating was carried out in the injection port of the GC apparatus (Model 7890B; Agilent) at 250°C for 5 min in splitless mode ...
-
bioRxiv - Microbiology 2020Quote: Proteins were detected using the following primary antibodies: anti-enterovirus VP1 clone 5-D8/1 antibody purchased from Dako (Denmark); Mouse anti-Ub ...
-
bioRxiv - Plant Biology 2021Quote: ... together with mass and retention time alignment (0.1 min and 5 ppm respectively) were done in Profinder B.07 software (from Agilent Technologies). Annotation was done based on the ‘find-by-formula’ algorithm ...
-
bioRxiv - Neuroscience 2020Quote: ... The internal lock masses (purine and HP-0921) and tune mix for calibrating the Q-TOF-MS (ESI-low concentration tuning mix) were purchased from Agilent Technologies (USA).
-
bioRxiv - Neuroscience 2020Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured from the plated astrocytes using the Seahorse XFe96 Cell Metabolism Analyzer (Agilent). Astrocytes were dissociated to single cells and plated at a density of 1×104 per well ...
-
bioRxiv - Microbiology 2021Quote: ... MDA-amplified environmental DNA isolated from the Lower Culex Basin at Yellowstone National Park (USA)17 was then amplified with Herculase polymerase (Agilent). Amplification of control and gap regions was then performed following the parameters shown in Suppl ...
-
bioRxiv - Systems Biology 2020Quote: Block 3-6s for the motif-rich core regions of our synthetic promoter constructs were amplified from a library synthesized by Agilent Technologies (LeProust et al. ...
-
bioRxiv - Microbiology 2020Quote: ... tricarboxylic cycle (TCA)) were quantified with GC-MS/MS (7890A GC coupled to a 7000 Quadrupole MS/MS, both from Agilent, Santa Clara ...
-
bioRxiv - Molecular Biology 2021Quote: ... The quantification of intracellular metabolites was performed with LC-MS/MS (UHPLC device from Agilent Technologies/QTRAP 5500; AB Sciex). Methodology details are available in De Souza et al ...
-
bioRxiv - Microbiology 2020Quote: Biotinylated RNA baits for enriching KSHV sequences in the library were those designed in [61] and were obtained from Agilent, Inc ...
-
bioRxiv - Cell Biology 2021Quote: ... CENP-A/H4 was expressed from a pST39 vector in BL21 (DE3) Codon Plus RIPL Escherichia coli (Agilent Technologies, 230280), grown in 2xYT medium (20 g/liter tryptone ...
-
bioRxiv - Cell Biology 2021Quote: ... The dDia2-CA mutant was created by first PCR-amplifying the forH gene (using forH4L and forH8 oligos) from GFP-dDia2 (Schirenbeck et al., 2005) then TA cloning the product using StrataClone (Agilent) to generate dDia2-SC ...
-
bioRxiv - Cell Biology 2021Quote: Ap-proximately 1000 ng of peptides from each biological replicate before SCX fractionation were first injected onto an Agilent 1290 Infinity UHPLC system (Agilent) on a 50-cm analytical column packed with C18 beads (Dr Maisch Reprosil C18 ...
-
bioRxiv - Cancer Biology 2021Quote: Using Agilent Technologies’ custom CGH-array format we arrayed 932 out of 1092 regions from the chromatin accessibility signature (Agilent probes were not available to cover the remaining 160 regions) ...
-
bioRxiv - Pathology 2022Quote: ... appropriate HRP-coupled secondary antibodies (rabbit anti-mouse, Cat. No. P0260, and goat anti-rabbit, Cat. No. P0448, from DAKO and rabbit anti-goat ...
-
bioRxiv - Cancer Biology 2022Quote: ... All uniquely labelled wells from each plate were pooled and multiplexed libraries were purified and quality controlled using TapeStation (Agilent) and Qubit fluorometer (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (catalog no ...
-
bioRxiv - Immunology 2022Quote: ... Mutations were introduced into VRC7480 either by site-directed mutagenesis using the QuikChange Lightning Site- Directed Mutagenesis Kit from Agilent Technologies (Catalog # 210518) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 450 ng of digested RNA was spiked with 50 ng of internal standard (13C stable isotope-labeled nucleosides from S. cerevisiae) and analyzed by LC-MS (Agilent 1260 Infinity system in combination with an Agilent 6470 Triple Quadrupole mass spectrometer equipped with an electrospray ion source (ESI)) ...
-
Metabolic switching and cell wall remodelling of Mycobacterium tuberculosis during bone tuberculosisbioRxiv - Microbiology 2022Quote: ... 1.0 μg of labelled cRNA from each sample and control were used for co-hybridization using the gene expression hybridization kit (Agilent) as per the manufacturer’s protocol ...
-
Metabolic switching and cell wall remodelling of Mycobacterium tuberculosis during bone tuberculosisbioRxiv - Microbiology 2022Quote: Data were extracted from the scanned tiff image for each sample using feature extraction software and analysed using Gene Spring GX software (Agilent). For statistical analysis ...
-
bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 nucleocapsid (EY-2A, a kind gift from Prof Alain Townsend) primary antibodies and appropriate HRP-conjugated secondary antibodies (DAKO). Chemiluminescence substrate (West Dura ...
-
bioRxiv - Genetics 2022Quote: Total RNA of each cell line was extracted 48 h after seeding and quality was assessed on electrophoretic spectra from a Fragment Analyzer (Agilent), analyzed with the PROSize software (v ...
-
bioRxiv - Developmental Biology 2022Quote: ... The pETM11-Sumo3-Tn5E54K,L372P plasmid was obtained from EMBL-Heidelberg and transformed into Escherichia coli BL21 (DE3) Gold cells (Agilent). Bacterial cultures were grown at 30◦C to a density of OD600=0.6-0.8 and protein expression was induced with 0.5 mM isopropyl-β-D-thiogalactoside (IPTG) ...
-
bioRxiv - Microbiology 2020Quote: ... A 515 bp homology region containing the mutation in PfDHODH (C276F) or the wildtype sequence from either SB1-A6 or D6 was cloned into StrataClone (Agilent) and then inserted using the NotI site of pDC2-U6A-hDHFR ...
-
bioRxiv - Biochemistry 2021Quote: The fluorophore-labeled single-stranded DNA oligonucleotide was separated from unlabeled oligonucleotide and unincorporated free fluorophore by C18 reverse-phase HPLC chromatography (Poroshell 120 EC-C18, Agilent). Labeled fractions were pooled and ethanol precipitated as described above and resuspended in aqueous solution (10mM Tris-HCl pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... The first reaction resulted in a plasmid containing 2,000 bp-long homologous arms (amplified from the genomic DNA of V. dahliae isolate Ls.17 using the Herculase II Fusion DNA polymerase; Agilent) flanking the neo cassette (conferring resistance to geneticin ...
-
bioRxiv - Neuroscience 2021Quote: ... RNA from cultured and transfected DRG cells was purified using the Absolutely RNA Nanoprep Kit (Agilent Tech., Santa Clara, CA). RNA quantity was measured using a NanoDrop 1000 (Thermo Fisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Secondary antibodies used were goat anti-rabbit BrightVision Horse Radish Peroxidase (Klinipath, Duiven, The Netherlands) and goat anti-mouse (#A32723, dilution 1:5000) obtained from Dako products (CA ...
-
bioRxiv - Cell Biology 2021Quote: ... The quantity and quality of RNA samples were analyzed using Agilent RNA 6000 Nano kit with the bioanalyzer from Agilent. RNA samples with RNA Integrity Number (RIN ...
-
bioRxiv - Cell Biology 2020Quote: ... All mutant constructs used in this study were generated from this construct either by a QuickChange II XL kit (Stratagene) or a Q5® Site-directed mutagenesis kit (New England Biolabs ...
-
bioRxiv - Neuroscience 2021Quote: ... Endogenous peroxidase and phosphatase activity in the tissues was quenched with a dual enzyme block from Dako (S2003, Agilent, UK). Sections were washed in 0.1% PBS-T and blocked with the relevant serum (goat ...
-
bioRxiv - Neuroscience 2021Quote: ... TDP-43N-Del was generated by deletion of the first 81 amino acids from the N-terminus of TDP-43WT using the QuickChange Site-Directed Mutagenesis Kit (Agilent).
-
bioRxiv - Evolutionary Biology 2022Quote: We isolated the plasmids containing the three segregating lacZ promoter variants from the MG1655 strain using StrataPrep Plasmid Miniprep Kit (Agilent). We also isolated the promoter- less vector pMV001 the same way ...
-
bioRxiv - Immunology 2022Quote: ... Total and free cholesterol content was determined by Gas Chromatography - Mass Spectrometry (7890B GS system, 5973 MS system, and 7693A automatic liquid sampler from Agilent; positive chemical ionization mode with 5% ammonia in methane as reaction gas) ...
-
bioRxiv - Cancer Biology 2022Quote: ... sgRNA sequence libraries were prepared from genomic DNA by two rounds of PCR using the Herculase II Fusion DNA Polymerase (Agilent). sgRNA sequences were amplified by the primers oMCB1562 and oMCB1563 and then indexed using the Illumina TruSeq LT adaptor sequences (AD002 ...
-
bioRxiv - Microbiology 2022Quote: The cyclohexane extracts of WAFs were analyzed by GC-MS/MS using a 7890A GC coupled with a 5975C triple-quadrupole-MS from Agilent Technologies.the 1 µ L of sample was injected into the GC inlet and heated according to the following oven temperature program ...
-
bioRxiv - Cancer Biology 2022Quote: ... V37W) from pLOC-NT5C2 plasmid by site directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent Technologies) according to manufacturer’s guidelines.
-
bioRxiv - Cell Biology 2020Quote: ... 2xmyc-RAB10-T73A and 2xmyc-RAB35-T72A were made by directed mutagenesis from the WT vectors using the QuickChange Lightning kit (Agilent). EGFP-RAB10 (Addgene#49472)(52) ...