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Citations for Agilent :
1351 - 1400 of 1560 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... total RNA isolated from jejunum was subjected to quality control analysis using an Agilent 2100 Bioanalyzer with RNA 6000 Nano Kits (Agilent, USA). After poly A enrichment the samples were fragmented and reverse-transcribed to generate complementary DNA for sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was performed in technical triplicates from at least 3 independent biological samples using the SYBR green Brilliant II fast kit (Agilent Technologies) on an Mx3005p apparatus (Agilent Technologies ...
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bioRxiv - Immunology 2023Quote: ... The Pfr2 copy number within each 20ng DNA skin sample was calculated from the standard curve using the ARIAMx qPCR software (Agilent, USA) as a proxy for estimated trypanosome load ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was isolated from total RNA and the mRNA integrity was confirmed using Agilent 1000 TapeStation (Agilent Technologies, Santa Clara, CA). Sequencing was performed on HiSeq 2500 Platform (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... The mutants in the present study were generated through use of SOE-PCR (as described earlier)19 or through use of the QuikChange® Site-Directed Mutagenesis Kit from Stratagene. Sequences of DNA primers used for PfuAmyGT and the point mutants are mentioned in Supplementary Information Table 1 (ST1).
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bioRxiv - Systems Biology 2023Quote: The kinetic data in this study are published and were acquired from xCELLigence assays using the xCELLigence Real Time Cell Analysis (RTCA) Single Plate device (Agilent Technologies) (Dankwa et al ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies). Lager scale transient transfections were purified on the Akta FPLC system using 1mL HiTrap MabSelect PrismA™ affinity chromatography resin (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... were generated by site directed mutagenesis on HA-NOD2 plasmid (a gift from Dana Philpott) using the QuickChange II Site-Directed Mutagenesis kit (Agilent Technologies) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and an aliquot from each sample was separated for RNA quality check using Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, US). The RNA samples were immediately stored at −80 °C for further analysis.
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bioRxiv - Biophysics 2024Quote: The recombinant human Cα subunit of cAMP-dependent protein kinase with the Phe to Ala mutation in position 100 (PKA-CF100A) was generated from the human PKA-Cα wild-type using Quik-Change Lightning mutagenesis kit (Agilent genomics). The key resource table lists the PCR primers used to modify the pET-28a expression vector encoding for the wild-type human PKA-Cα gene (PRKACA – uniprot P17612 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Ultrapure ammonium acetate salt was purchased from VWR Scientific (San Francisco, CA) and tuning mix for ESI-time-of-flight mass spectrometry was purchased from Agilent (Santa Clara, CA). 4’OH-diclofenac and 4’OH-5-chloro-diclofenac were a gift from Dr ...
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bioRxiv - Immunology 2024Quote: ... or IL-4 and ABS-hi using Extracellular Flux Assay kits (XFE96 Flupax mini 102601-100 kit, Seahorse XF DMEM assay medium pack 103680-100 kit, XF cell mitostress test 103015-100 kit; all from Agilent Technologies) and Seahorse XF Pro Analyzer according to manufacturer’s recommendations and previously published protocol ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from cells under the indicated experimental conditions using a Qiagen miRNAeasy kit (Valencia) and measured using an Agilent Bioanalyzer (Agilent Technologies). Illumina Novaseq 6000 libraries were prepared and sequenced by Novogene (CA ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 libraries were generated from murine tissue samples and libraries were quantified using the Agilent DNA 1000 kit (Agilent Technologies, USA).
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bioRxiv - Evolutionary Biology 2023Quote: The total RNA extracted from the floral meristem of species listed in Supplementary Table 1 were checked for quality using RNA ScreenTape (Agilent Technologies) and Qubit 2.0 fluorometer was used for determining the concentration ...
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bioRxiv - Genomics 2023Quote: ... and vas deferens total RNA with an RNA integrity number (RIN) inferred from a Bioanalyzer RNA 600 Nano assay (Agilent Technologies). For subsequent analyses ...
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bioRxiv - Neuroscience 2024Quote: RNA from sorted cells were analyzed on the Agilent 2100 Bioanalyser system using the Eukaryote Total RNA Pico kit (Agilent Technologies) and the mean RIN number was 9.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Site-specific mutagenesis was performed according to standard procedures of the QuickChange Site-Directed Mutagenesis Kit purchased from Stratagene (GE Healthcare) or Mut Express II (Vazyme Biotech) ...
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bioRxiv - Bioengineering 2022Quote: The pulse power supplier was programmed to generate square monophasic waves of 1500 V to the entire setup, and the waveforms were obtained from probe P6015A (Tektronix, ltd) and oscilloscope MS0-X3204 (bandwidth 200MHz, Agilent, ltd). Then square waveform electrical pulses with pulse width of 20 μs and pulses duration of 5 s were delivered into each well at a frequency of 10 Hz with variable electric intensity ...
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bioRxiv - Immunology 2022Quote: Biotinylated MHC monomers with human β2-microglobulin specific for H-2Ld β-galactosidase (TPHPARIGL) were obtained from the NIH Tetramer Core Facility and tetramerized with streptavidin-PE (Prozyme). Decoy reagent was made according to published protocols (20) ...
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bioRxiv - Developmental Biology 2022Quote: ... Deletion of putative TF binding sites from those enhancers were performed using QuickChange II XL site-directed mutagenesis kit (Agilent Technologies). All constructs were confirmed by Sanger Sequencing (Quintara Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... the gentamicin resistance marker (PflaB-aaC1) was amplified from pMH105 (20) by PCR and then TA-cloned into pSC-A-amp/kan (Agilent Technologies). Following confirmation of the TA clones ...
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bioRxiv - Neuroscience 2022Quote: ... The contents of each well after the ThT-based assay were centrifuged at 21k x g for 20 minutes and the supernatant from each well was added to individual aliquots in the autosampler of the Agilent 1260 Infinity HPLC system (Agilent Technologies). 20 μL of each sample was injected onto an Advance Bio SEC column ...
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bioRxiv - Immunology 2023Quote: ... 1.5 µM FCCP and 1 µM Rotenone/1 µM Antimycin A from Seahorse XF Cell Mito Stress Test (103015-100, Agilent Technologies), and read using Seahorse XFe96 Analyzer (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: PTEN monoubiquitination mutants PTEN-K13R and PTEN-K289R were generated from GFP-PTEN by site directed mutagenesis using QuickChange XL Site Directed Mutagenesis Kit (Agilent technologies). Table S1 includes the primer sequences.
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bioRxiv - Genomics 2023Quote: ... PCR amplified libraries were purified from 8% PAGE gels and subsequently analysed with the Agilent High Sensitivity DNA assay (Agilent, Bioanalyzer).
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bioRxiv - Biochemistry 2022Quote: The following materials were obtained from the cited sources and used in this study: Pfu Ultra II Fusion HS DNA Polymerase (Agilent Technologies); restriction enzymes and DNA ligation kit ver.2 (Takara Bio) ...
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bioRxiv - Cancer Biology 2022Quote: ... The DNA from the released chromatin in the supernatant was purified and then quantified using Qubit dsDNA HS assay kit (Agilent Technologies). CUT&RUN libraries were constructed using NEBNext Ultra II DNA library preparation kit as described previously (Skene et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... were constructed from pcDNA3.1+-C-(K)-DYK-EBP plasmids using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, #210519) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... PHA was extracted from lyophilized sludge using acidified methanol (3% sulfuric acid) containing chloroform and analyzed using a gas chromatography-mass spectrometry (Agilent, USA). Glycogen in sludge was extracted in a similar way but using 0.9M HCl and analyzed using a liquid chromatography-mass spectrometer (Thermo Scientific ...
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bioRxiv - Physiology 2023Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All the fusion constructs were made from the appropriate backbone vector with the QuikChange Lightning Multi Site Directed Mutagenesis kit (Agilent Technologies). Reporters were sequenced from Genewiz before transfecting the parasites ...
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bioRxiv - Cell Biology 2023Quote: ... Samples from the primary human monolayer experiment were fixed and immunostained for insulin with guinea pig anti-insulin primary antibody (Dako A0564) and Alexa-Fluor 568 goat anti-guinea pig secondary antibody (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted as mentioned above from P10 control and microglia depleted retinas and the RNA integrity was examined using by Bioanalyzer (Agilent 2100). The RIN values of RNA used for cDNA library construction were >9.0 ...
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bioRxiv - Cancer Biology 2024Quote: Oxygen consumption rate (OCR) and Extra-Cellular Acidification rate (ECAR) experiments were performed using the XF96pro apparatus from Seahorse Bioscience (Agilent). Cells were seeded to ∼90% confluence for each condition in RPMI ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2uL aliquots from each plated sample were diluted 1:9 on a Bravo liquid handling platform (Agilent, Santa Clara, CA, USA). 5uL aliquots from each diluted sample were pipetted into 384-well plates for mNGS library preparation ...
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bioRxiv - Cell Biology 2024Quote: hiPSC-CMs from micropatterned or non-patterned conditions (day 42) were seeded into VN-coated Seahorse 96 assay plates (Seahorse Bioscience, Agilent Technologies France ...
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bioRxiv - Microbiology 2024Quote: ... Inactivating mutations of the HP1α-interacting chromatin protein mimicry in JC virus Agnoprotein were introduced using the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent (#200521) and the appropriate primers (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: pB-T-PAF-VLDLR(1-797)Δ(751-779)-C-tag was obtained by PCR amplification of VLDLR from human placenta cDNA (Edge Biosystems) using Herculase-II Fusion DNA polymerase (Agilent Technologies), followed by Gibson assembly with pB-T-PAF using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Microbiology 2024Quote: ... Cyanine 3 (Cy3)-labeled complementary RNA was synthesized from 50 ng of total RNA using Low Input Quick Amp WT Labeling Kit (Agilent Technologies) and subjected to microarray hybridization ...
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bioRxiv - Biochemistry 2024Quote: ... pT7-7 α-syn FL plasmid (a gift from Hilal Lashuel, Addgene66) was transformed into BL21-Gold (DE3) competent Escherichia coli (E. coli) cells (Agilent Technologies) according to the manufacturer’s instructions and previous methodology44 ...
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bioRxiv - Biochemistry 2024Quote: ... coupled to quadruple time of flight mass spectrometry (1290 UHPLC / 6550 iFunnel QTOF-MS from Agilent Technologies, Santa Clara, CA, USA) as previously reported by Salehi et al. ...
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bioRxiv - Plant Biology 2024Quote: ... The absorption spectrum (from 750 to 600 nm) of the supernatant was measured using a Cary 100 UV-Vis spectrophotometer (Agilent Technologies). The chlorophylls concentration of the pure protein extract ...
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bioRxiv - Neuroscience 2024Quote: The mutant PDI-Ala256 protein was prepared from the full-length wild-type human PDI cDNA using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). For protein purification ...
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bioRxiv - Molecular Biology 2024Quote: ... Signal intensity values were extracted from the resulting single channel TIFF images using Agilent feature extraction software (Agilent Feature Extraction 11.0.1.1) with default parameters (AgilentG3_HiSen_GX_1color) ...
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bioRxiv - Microbiology 2024Quote: ... A mean concentration of the cDNA in each well was calculated using measurements from the Bioanalyzer 2100 (Agilent Technologies, Waldbronn, Germany) and cDNA concentrations were normalised to 1.4 ng/μl with nuclease-free water using the automated liquid handler Mosquito ...
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bioRxiv - Biochemistry 2024Quote: ... The NDec1-98 mutant was prepared using purified N-His6 Dec plasmid DNA from a DH5α bacterial culture that was obtained using a StrataPrep plasmid miniprep kit (Agilent Technologies). Replacing codon 99 with a stop codon was accomplished by site-directed mutagenesis ...
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bioRxiv - Synthetic Biology 2024Quote: ... The targeted region was amplified from collected genomic DNA or reference plasmid using high fidelity Herculase II Fusion DNA Polymerase (Agilent, 600675). The input template for PCR was 100 ng of genomic DNA or 1 ng of reference plasmid in a final reaction volume of 50 μl ...
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bioRxiv - Biochemistry 2024Quote: ... site-directed mutagenesis of the pET15b-NtcA plasmid was carried out using the Quickchange II system (from Stratagene, La Jolla, CA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the DV200 quality metric of RNA from FFPE pancreas tissue was first verified using RNA 6000 Nano Kit (Agilent #5067-1511) according to manufacturer’s instructions ...