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Citations for Agilent :
901 - 950 of 1553 citations for Mouse KIF26B shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... In order to generate the mutant expression plasmids for BMAL2 we used the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (210515, Agilent). The bHLH mutant was generated by converting an arginine to alanine (OaBMAL2_R88A ...
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bioRxiv - Genomics 2021Quote: The different Sen1 HD versions were fused to a C-terminal His8-tag coupled to Vibrio cholerae MARTX toxin cysteine protease domain (61)and expressed from the T7 promoter (see plasmids in table S6) in Escherichia coli strain BL21 (DE3) CodonPlus (Stratagene). Overexpression was induced by growth in auto-inducing medium (62 ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids with mutant YBEY alleles (pcDNA4-YBEY-R55A and pcDNA4-YBEY-H128A) were generated by QuickChange site-directed mutagenesis (Agilent) with the following pairs of primers ...
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bioRxiv - Biochemistry 2021Quote: ... 67)) plasmid as PCR template Bri2 BRICHOS D148N was obtained with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, US), and the DNA sequence was confirmed (GATC Bioteq ...
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bioRxiv - Biochemistry 2021Quote: ... All reactions were carried out using a TGradient (Biometra) thermocycler and resulting mutant plasmids transformed into electro competent BL21-Gold (DE3) cells (Agilent). The integrity of all constructs and PK mutations were confirmed by sequencing (Genewiz).
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bioRxiv - Biophysics 2020Quote: A Pol I expression vector was generated from the plasmid pXS67 (Yale Coli Genetic Stock Center, Strain CJ803) by site-directed mutagenesis using a QuikChange kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... first an AgeI cut-site was knocked into the pcDNA3.1-Myoferlin-HA plasmid immediately prior to the transmembrane domain by site-directed mutagenesis (Agilent: 210518). Second ...
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bioRxiv - Genomics 2020Quote: BG4-encoding plasmid (kindly provided by Professor Shankar Balasubramanian, University of Cambridge, UK) was transformed into BL21(DE3) competent cells (Stratagene) which were cultured in TY medium (1.6% tryptone peptone ...
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bioRxiv - Microbiology 2021Quote: ... Site-specific mutants in ftsA were constructed by site-directed mutagenesis of ftsA expression plasmids using the QuikChange II XL mutagenesis system (Agilent) and confirmed by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein mutants were obtained by PCR-based site-directed mutagenesis with the pET-M11-RRM12 (12-235) plasmid as a template according to the QuikChange protocol (Stratagene) and the primers listed in Table S1 ...
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bioRxiv - Microbiology 2021Quote: Mutations were introduced into the HA plasmid of A/Hong Kong/1/1968 using a site-directed mutagenesis kit (QuikChange; Stratagene). 2:6 recombinant IAVs containing wt and modified HA of A/Hong Kong/1/1968 ...
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bioRxiv - Genetics 2020Quote: Single variants were introduced by mutagenesis of the Tile 3-KCNH2 plasmid (Table S1) using the Quikchange Lightning Multi kit (Agilent), with 1 primer per variant ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid pAscA was constructed by amplifying the DNA encoding AscA containing PciI and BamHI sticky ends and ligating into plasmid pTrc99a (Stratagene) between the NcoI and BamHI sites ...
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bioRxiv - Biochemistry 2022Quote: Human ALDH9A1 mutants were generated from the template plasmid (EX-Z3075-M29) described above using a QuickChange XL II Site-Directed Mutagenesis Kit (Agilent). Primers were designed with the corresponding mutations as prescribed by the QuickChange Primer Design tool (https://www.agilent.com/store/primerDesignProgram.jsp ...
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bioRxiv - Genetics 2022Quote: ... the other mtr4 mutant plasmids were constructed by site-directed mutagenesis of the MTR4 HIS CEN6 plasmid (pAC4096) with the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the corresponding missense mutations ...
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bioRxiv - Microbiology 2022Quote: ... The obtained amplicons were blunt-cloned into an EcoRV-digested pBluscript KS+ plasmid and transformed into the Sure Escherichia coli strain (Stratagene), cultured at 30 °C.
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bioRxiv - Neuroscience 2023Quote: ... constructs cloned in AAV-hSyn vectors were transfected with RC/DJ and adenovirus-helper plasmid into the AAV-293 cells (#240073, Agilent) using calcium phosphate precipitation method ...
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bioRxiv - Microbiology 2022Quote: ... Mutations were made on pDUAL plasmids encoding the PR8 gene (64) using a QuikChange II site directed mutagenesis kit (Agilent) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Plasmids were transformed and expressed in the Escherichia coli strain BL-21 (DE3) RIL cells (Agilent, Santa Clara, CA, USA). For protein expressions ...
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bioRxiv - Cell Biology 2022Quote: ... The wild type form of RNAi-resistant MUS81 ORF cloned into the pEF1a-IRES-NEO plasmid was subjected to SDM (Quickchange II XL – Stratagene) to introduce the T86 or S97 mutations ...
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bioRxiv - Cell Biology 2024Quote: The fluorescence from mNG21-10/mNG211 complementation was visualized the day after transfection with the pH2B-mNG211 plasmid using a Cytation 5 microscope (Agilent) equipped with a 20x/0.45 NA phase contrast objective ...
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bioRxiv - Biochemistry 2024Quote: ... The calibrated concentrations of the cleavage product bands (electrogram peak areas) were used to calculate percent cleavage from total plasmid band intensity using TapeStation Analysis software (Agilent).
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bioRxiv - Biophysics 2024Quote: ... The pBAD30-ClsA (EE) G51A mutant plasmid was generated from the pBAD30- ClsA (EE) template with QuikChange II Site-Directed Mutagenesis Kit (Agilent). Two residues ...
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bioRxiv - Biochemistry 2024Quote: All of the mutations were introduced into a plasmid backbone expressing 6xHis tagged pNL4-3-derived IN by QuikChange site-directed mutagenesis kit (Agilent). The plasmids containing full-length WT and mutants were transformed into BL21 (DE3 ...
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bioRxiv - Microbiology 2024Quote: ... Primers containing the desired deletions and complement were used to amplify the entire plasmid sequence using PfuUltra II fusion HS DNA polymerase (Agilent). After PCR ...
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bioRxiv - Cell Biology 2023Quote: ... The mutations corresponding to the MARylation sites of RACK1 (D144N/E145Q/D203N) were introduced into the pCMV3-RACK1 plasmid using the QuikChange Site-Directed Mutagenesis kit (Agilent).
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bioRxiv - Microbiology 2023Quote: ... The resulting pHD1344tub(PAC)-mt-LAF3-Cterm3V5 plasmid was then used as a template for site-directed mutagenesis of D211 (QuikChange II kit; Agilent) using forward and reverse primers listed in Supplemental Table 1 ...
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bioRxiv - Biophysics 2023Quote: ... derivative encoding two cysteine residues at positions 366 and 511 [constructed from plasmid pGEMD (-Cys)46 by use of site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit; Agilent) to replace codons 366 and 511 by a codon encoding cysteine residue] ...
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bioRxiv - Microbiology 2023Quote: ... A R43A PEXEL mutation in UIS21-70-mNG was generated in this plasmid using a QuikChange Lightning Multi Site Directed Mutagenesis kit (Agilent) and the primer P9 ...
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bioRxiv - Microbiology 2023Quote: ... All the H2 mutant constructs were subsequently derived from the pEF6-H2R plasmid using a QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent), with mutation accuracy confirmed by sequencing (Genomics Inc. ...
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bioRxiv - Genetics 2023Quote: ... the Prab-3p::hpk-1 plasmid was used and mutations were performed with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All the assembled plasmids were validated by sequencing prior to microinjection ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting vectors were subjected to sequencing to ensure that no mutations were introduced into the WRN sequence in the plasmid used for transforming BL21 cells (Stratagene). Expression of GST and GST-fusion proteins were induced upon addition of 1 mM isopropyl-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... R448A and N455A were introduced in the pETNKI-his-JBP1 JDBD plasmid using a modified QuickChange site-directed mutagenesis method (Agilent). Forward and reverse mutagenesis primers containing the single point mutations were purchased from ThermoFisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... The cells were transfected with 100 ng of NF-κB firefly luciferase reporter plasmid p(NF-κB)3-Luc (Stratagene) and 10 ng of Renilla luciferase reporter plasmid pRL-RSV (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... the corresponding pAAV plasmid construct along with AAV helper plasmids encoding the structural elements were transfected using polyethylenimine (PEI) into the AAV-293 cell line (CVCL_6871, Agilent, USA). The day before transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Site-directed mutagenesis was performed based on PCR of the full-length plasmid by using Pfu Turbo DNA polymerase (Stratagene). The entire cDNA was sequenced ...
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bioRxiv - Molecular Biology 2023Quote: ... mutant plasmids encoding the different RNA exosomopathy amino acid substitutions were generated by site-directed mutagenesis of the respective wild-type plasmids (pAC3656, pAC3652, pAC3482) using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the desired missense mutations as previously described [33 ...
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bioRxiv - Microbiology 2024Quote: To detect changes in neutralisation potency single point mutations were introduced into env encoding plasmids for PV production using the QuikChange Lightning Site-Directed Mutagenesis (SDM) kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: Donor plasmid PAM sites were mutated using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The gRNA-encoding plasmids pU6-BbsI-chiRNA-RhoGEF2 and pU6-BbsI-chiRNA-Cysts were made by insertion of gRNA oligos into pU6-BbsI-chiRNA (Addgene #45946 ...
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bioRxiv - Biophysics 2024Quote: ... Point mutations (FliG-G214S or FliG-G215A) in plasmid pRO301 were introduced using the QuikChange site-directed mutagenesis method (Agilent).
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bioRxiv - Biochemistry 2024Quote: The HAdV5HVR7 vectors (“1st generation” vectors) were obtained by cloning the gene of interest into the pAdEasy-1 plasmid system from Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... is a pCEP4-based plasmid that contains the humanized enhanced green fluorescent protein (hrGFP) coding sequence from phrGFP-C (Agilent) under the control of the pCEP4 CMV promoter and SV40 polyadenylation signal (39).
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bioRxiv - Molecular Biology 2024Quote: The CtIP-S276A variant was prepared by mutating the respective wild-type pFB-2xMBP-CtIP-10xhis plasmid by QuickChange site-directed mutagenesis kit following manufacturer’s instructions (Agilent Technology). The wild-type protein ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with secondary antibody polyclonal rabbit anti-mouse HRP-conjugate (cat.P0260, Dako, Agilent Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... WES capture was done using the Sureselect Mouse All Exon 50 Mb (Agilent technologies). Paired-end sequencing was performed on an AB Hiseq 2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Molecular Biology 2021Quote: ... horseradish peroxidase (HRP)-conjugated rabbit or mouse antibodies (Dako, cat# P0399 or P0447, respectively) were used for 1 h at room temperature ...