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Citations for Agilent :
751 - 800 of 1488 citations for Mouse KIF26B shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Molecular Biology 2023Quote: ... megaprimers were synthesized and cloned into the TFAM-mScarlet_pWPXL plasmid following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of pcDNA3.1-NS3-mCherry-HIS plasmid was done by using PfuUltra HotStart DNA Polymerase (Agilent, Cat #: 600390) and forward and reverse primers (table 2 ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid containing an IDE E111Q mutant was constructed using a QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) and the pProEx-IDE-wt plasmid ...
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bioRxiv - Physiology 2023Quote: ... SIK3-Flag-S884A and S884D plasmids were generated by introducing site-directed mutations into SIK3-Flag plasmid using the QuikChange II XL site-directed mutagenesis kit according to manufacturer’s instruction (Agilent). Lenti-Puro-SIK3-Flag plasmid was constructed by subcloning the “mSIK3-3xFlag” coding sequences (CDS ...
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bioRxiv - Neuroscience 2023Quote: ... nucleotides 214–990) was amplified by PCR and cloned into the pBluscript II KS (−) plasmid (Stratagene, La Jolla, CA). Antisense RNAs were synthesized from linearized plasmids using T3 RNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The pHD1344tub(PAC)-Nterm3V5-A3 plasmid was mutagenized using the QuickChange Lightning site-directed mutagenesis kit (Agilent, product #210518) and primers listed in Table S2 ...
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bioRxiv - Biochemistry 2023Quote: ... COS1 cells were transfected with pAdTrack-CMV expression plasmids (Table S1) using the GeneJammer transfection reagent (#204130, Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The PAM sequences within each donor plasmid were mutated using site directed mutagenesis with Pfu Turbo (Agilent, Cat# 600250) to avoid targeting of the donor plasmid by Cas9 nuclease ...
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bioRxiv - Immunology 2023Quote: ... SAMD9L-F886Lfs*11-E198A/D243A were generated from pMAX:mTFP1-SAMD9L plasmid using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... a codon-optimized gene block was cloned into the 6xHis_1B LIC plasmid (gift from Scott Gradia, UC Berkeley) and transformed into Arctic Express bacteria (Agilent). Expression was induced with 0.3 mM IPTG for 16 hr at 20°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reference plasmids containing PA tag sequence were constructed in pBluescript II SK (+) vector (Agilent, Palo Alto, CA, USA). One μg of guide RNA inserted vector and 1.0 μg of reference plasmid were co-transfected into EGRG01 embryonic stem (ES ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids for His6-ZZ-TEV-MZT2 and GST-GCP2-NHD were co-transformed into BL21-CodonPlus (DE3)-RIL (Stratagene) cells ...
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bioRxiv - Neuroscience 2023Quote: AAV plasmids carrying cDNA for hIL-6 downstream of a CMV promoter were co-transfected with pAAV-RC (Stratagene) encoding the AAV genes rep and cap ...
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bioRxiv - Genetics 2024Quote: ... the E171Q variant was introduced into an SCN5A expression plasmid (pCDNA3.1) using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent). HEK293T cells were grown at pH 7.4 in filtered sterile DMEM nutrient medium (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... R80A and H71R) or pLRGatewayIRESeYFP-Vpr plasmids were synthesised using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions and primers listed in Table 2 ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids coding for hSSB1 variants were generated from the Y85C substitution-corrected pET29a-hSSB1 vector using the QuikChange (Agilent) mutagenesis kit ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were incubated with FITC-conjugated goat F(ab’)2 anti-mouse (Dako), diluted 1/100 in RPMI-H.
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bioRxiv - Cell Biology 2020Quote: ... BrdU was immunofluorescently labeled with a mouse anti-BrdU antibody (DAKO, clone Bu20a) and a fluorescein-conjugated donkey anti-mouse antibody (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... and the rabbit anti-mouse-IgG conjugated to horseradish peroxidase (1:10’000, Dako).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and secondary HRP-conjugated goat anti-mouse Ab (Dako, Santa Clara, CA, USA), as described previously 16–18 ...
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bioRxiv - Immunology 2019Quote: ... An isotype control for IL-18 staining was performed using mouse IgG (Dako). Samples were washed thrice with blocking buffer and incubated for 2 hr with anti-rat Alexa Fluor® 488- and anti-rabbit Alexa Fluor® 594-conjugated secondary antibodies (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-human CD68 (Dako, clone PG-M1, 1:750, pH 6 retrieval), mouse anti-human CD20 (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-HLA-DR (clone TAL.1B5, Dako Agilent, Santa Clara, CA, USA); rabbit anti-IBA1 (Polyclonal IgG ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-HLA-DR (clone TAL.1B5, Dako Agilent, Santa Clara, CA, USA); rabbit anti-IBA1 (Polyclonal IgG ...
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bioRxiv - Biochemistry 2020Quote: ... Horseradish peroxidase-conjugated secondary anti-mouse and anti-rabbit antibodies (Dako, Glostrup, Denmark) were used at concentrations of 1:5000 and 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies used were HRP-conjugated anti-mouse or anti-rabbit (Dako). Signals were imaged using the Fujifilm LAS-3000 system and quantified with ImageJ software.
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bioRxiv - Immunology 2022Quote: ... a mouse anti-CD20 antibody (M0755, clone L26, Dako, Santa Clara CA, USA), or for macrophages 1 hour at room temperature with a mixture of mouse anti-CD68 (CM033B ...
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bioRxiv - Physiology 2022Quote: ... or goat anti-mouse Ig HRP-conjugated (1:5000, catalog no. P0447, Dako) or goat anti-rat IgG HRP-conjugated (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... microglial lysosomes were stained using CD68 (mouse anti-human monoclonal primary antibody, Dako M0876,1:100 ...
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bioRxiv - Immunology 2021Quote: ... isotype mouse IgG1 (Clone #DAK-GO1, Catalog Number: X0931, Dako Cytomation, Glostrup, Denmark) for CXCL12 and BRCA2 stainings ...
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bioRxiv - Microbiology 2020Quote: ... we used a peroxidase-coupled goat anti-mouse antibody (Dako, P0447, 1:5000).
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bioRxiv - Cancer Biology 2020Quote: ... cytotoxic T lymphocytes (anti-CD8 mouse IgG1κ, 1:250, clone C8/144B, Dako), and B lymphocytes (anti-CD20 mouse IgG2a ...
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bioRxiv - Microbiology 2020Quote: ... and 1:20000 dilution of HRP-labelled secondary rabbit anti-mouse antibody (DakoCytomation). The membrane was incubated with ECL substrate (WesternBright Quantum ...
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bioRxiv - Microbiology 2020Quote: ... and using Cytokeratin clone AE1/AE3 mouse monoclonal antibody (Dako/Agilent cat #M3515) at a 1:100 dilution to identify epithelial cells ...
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bioRxiv - Microbiology 2020Quote: ... and using Cytokeratin clone AE1/AE3 mouse monoclonal antibody (Dako/Agilent cat #M3515) at a 1:100 dilution to identify epithelial cells ...
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bioRxiv - Microbiology 2021Quote: ... EnVision+ system-HRP-labeled polymer anti-mouse secondary antibody (Dako, Carpinteria, CA, USA) was used ...
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bioRxiv - Cancer Biology 2021Quote: ... and CD163 (mouse monoclonal, clone 10D6, BioSB) on a Autostainer Link48 platform (Dako) using the EnVision FLEX® system (Dako ...
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bioRxiv - Cancer Biology 2019Quote: ... and monoclonal mouse anti-Ki-67 antibody (M7240, Agilent, Santa Clara, CA, USA). Hematoxylin (Vector Laboratories ...
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bioRxiv - Genomics 2019Quote: ... and SureSelect XT Mouse All Exon v.1 probe (Agilent Technologies, 5190-4642).
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bioRxiv - Developmental Biology 2019Quote: ... were subjected to a SurePrint G3 Mouse GE v2 8×60K Microarray (Agilent). The microarray data obtained for this study are deposited in the Gene Expression Omnibus database (GEO ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish Peroxidase (HRP) linked secondary anti-mouse/rabbit secondary antibodies (Dako, Aglient, UK) were used at 1:1500 for 2h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and α-CD19 (clone HD37; Dako [α-human]; clone SJ25-C1 [α-mouse]) were added to 5 × 108 cells for 5 min at 37° ...
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bioRxiv - Genomics 2019Quote: ... and mouse monoclonal anti-human CD8 (DAKO, clone C8/144B, dilution 1:25) at 1 hour RT ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated with appropriate secondary antibodies (Polyclonal goat anti-mouse (Dako, P0447) and polyclonal goat anti-rabbit (Dako ...
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bioRxiv - Physiology 2021Quote: ... Anti-human CD68 (mouse monoclonal IgG3, clone PG-M1, Dako, dilution 1/100) and anti-human IL-1β (rabbit polyclonal antibody ...
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bioRxiv - Microbiology 2021Quote: ... mouse monoclonal anti-vimentin antibody (Cat# GA630, RRID:AB_2827759, Agilent (St. Clara, CA, USA)) and rabbit monoclonal antibody against GAPDH (D16H11 ...
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bioRxiv - Molecular Biology 2021Quote: ... Alexa Fluor 488 goat anti-mouse IgG (rabbit polyclonal, P0449: Dako, Glostrup, Denmark), and Alexa Fluor 594 goat anti-rabbit IgG (Dako).
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bioRxiv - Molecular Biology 2020Quote: ... and pan-cytokeratin AE1/AE3 anti-mouse (1:200, DAKO Cytomation, Catalog#: M3515) using the following diluent ...